scholarly journals Impact of Alkaline Pretreatment Condition on Enzymatic Hydrolysis of Sugarcane Bagasse and Pretreatment Cost

2021 ◽  
Author(s):  
Chaojun Wang ◽  
Wei Qi ◽  
Cuiyi Liang ◽  
Qiong Wang ◽  
Wen Wang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Linear Trend ◽  
Alkaline Pretreatment ◽  
Practical Application ◽  
Severity Factor ◽  
Pretreatment Condition ◽  
Maximum Value ◽  
Combined Severity Factor ◽  
Hydrolysis Of ◽  
Temperature Time

Abstract A combined severity factor (RCSF) which is usually used to evaluate the effectiveness of hydrothermal pretreatment at above 100 ºC had been developed to assess the influence of temperature, time and alkali loading on pretreatment and enzymatic hydrolysis of lignocellulose. It is not suitable for evaluating alkaline pretreatment effectiveness at lower than 100 ºC. According to the reported deducing process, this study modified the expression of RCSF = log[CnOH- x t x e(Tr-Tb)/14.75] as RCSF = log{COH- x t x e[-13700/(Tr+273)+36.2]} which is easier and more reasonable to assess the effectiveness of alkaline pretreatment. It showed that RCSF exhibited linear trend with lignin removal, and quadratic curve relation with enzymatic hydrolysis efficiency (EHE) at the same temperature. The EHE of alkali-treated SCB could attain the maximum value at lower RCSF, which indicated that it was not necessary to continuously enhance strength of alkaline pretreatment for improving EHE. Within a certain temperature range, the alkali loading was more important than temperature and time to influence pretreatment effectiveness and EHE. Furthermore, the contribution of temperature, time and alkali loading to pretreatment cost which was seldom concerned was investigated in this work. The alkali loading contributed more than 70% to the pretreatment cost. This study laid the foundation of further optimizing alkaline pretreatment to reduce cost for its practical application.

Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Fokko Schütt ◽  
Jürgen Puls ◽  
Bodo Saake
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
Steam Pretreatment ◽  
Alkaline Extraction ◽  
Poplar Wood ◽  
Steam Refining ◽  
Severity Factor ◽  
Short Rotation ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

Abstract Steam refining was investigated as a pretreatment for enzymatic hydrolysis of poplar wood from a short rotation plantation. The experiments were carried out without debarking to use an economically realistic raw material. Steam refining conditions were varied in the range of 3–30 min and 170–220°C, according to a factorial design created with the software JMP from SAS Institute Inc., Cary, NC, USA. Predicted steaming conditions for highest glucose and xylose yields after enzymatic hydrolysis were at 210°C and 15 min. Control tests under the optimized conditions verified the predicted results. Further pretreatments without bark showed that the enzymes were not significantly inhibited by the bark. The yield of glucose and xylose was 61.9% of theoretical for the experiments with the whole raw material, whereas the yield for the experiments without bark was 63.6%. Alkaline extraction of lignin from the fibers before enzymatic hydrolysis resulted in an increase of glucose yields from mild pretreated fibers and a decrease for severe pretreated fibers. The extracted lignin had a high content of xylose of up to 14% after very mild pretreatments. On the other hand, molecular weights of the extracted lignin increased substantially after pretreatments with a severity factor above 4. Hence, alkaline extraction of the lignin seems only attractive in a narrow range of steaming conditions.

Enhanced enzymatic hydrolysis of spruce by alkaline pretreatment at low temperature

2008 ◽  
Vol 99 (6) ◽  
pp. 1320-1328 ◽  
Author(s):  
Yulin Zhao ◽  
Ying Wang ◽  
J.Y. Zhu ◽  
Art Ragauskas ◽  
Yulin Deng
Keyword(s):  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Alkaline Pretreatment ◽  
Hydrolysis Of

scholarly journals Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass

Energies ◽  
2021 ◽  
Vol 14 (23) ◽  
pp. 8053
Author(s):  
Eun-Young Park ◽  
Jung-Kyu Park
Keyword(s):  
Enzymatic Hydrolysis ◽  
Saccharina Japonica ◽  
Reducing Sugar ◽  
Single Treatment ◽  
Acid Pretreatment ◽  
Experimental Procedure ◽  
Independent Variables ◽  
Hcl Concentration ◽  
Hydrolysis Of ◽  
Temperature Time

This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RSy). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/wbiomass Celluclast® 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl concentration). Using this experimental procedure, the yields of hydrothermal acid pretreatment, enzymatic hydrolysis, and sequential hydrolysis were 115.6 ± 0.4 mg/g, 117.7 ± 0.3 mg/g, and 183.5 ± 0.6 mg/g, respectively. Our results suggested that sequential hydrolysis of hydrothermal acid pretreatment and enzymatic hydrolysis was more efficient than their single treatment.

scholarly journals Phenomenological Modeling of Formic Acid Fractionation of Sugarcane Bagasse by Integration of Operation Parameters as an Extended Combined Severity Factor

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2753
Author(s):  
Xiaogang Chang ◽  
Jingzhi Zhang ◽  
Ruchun Wu ◽  
Xuebing Zhao
Keyword(s):  
Formic Acid ◽  
Sugarcane Bagasse ◽  
Severity Factor ◽  
Logistic Equations ◽  
Inflection Points ◽  
Combined Severity Factor ◽  
Cellulose Digestibility ◽  
Biomass Components ◽  
Operation Parameters ◽  
Hydrolysis Of

In order to more conveniently simulate and optimize the solubilization of sugarcane bagasse components during formic acid (FA) fractionation, an extended combined severity factor (CSFext) was defined to integrate various operation parameters as a single factor. Two phenomenological models based on Arrhenius and Logistic equations were further used to describe the phenomenological kinetics. Different data-processing methods were compared to fit the severity parameters and model constants. Both Arrhenius-based and Logistic-based models show satisfying fitting results, though the values of Arrhenius-based CSFext (A-CSFext) and Logistic-based CSFext (L-CSFext) were somewhat different under the same fractionation condition. The solubilization of biomass components increased with CSFext, but two distinct stages could be observed with inflection points at A-CSFext of 42 or L-CSFext of 43, corresponding to bulk and residual solubilization stages, respectively. For the enzymatic hydrolysis of cellulosic solids, the highest initial enzymatic glucan conversion (EGC@6h) was obtained at A-CSFext of 39–40 or A-CSFext of 40–41; however, for a long hydrolysis period (72 h), relatively high glucan conversion (EGC@72h) was observed at A-CSFext of 42–43 or A-CSFext of 43–44. Post-treatment for deformylation with a small amount of lime could help to recover the cellulose digestibility.

scholarly journals Process simulation for xylitol production from brewer’s spent grain in a Colombian biorefinery. Part 1: Xylose production from arabinoxilans extracted by the alkaline pretreatment of BSG

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Andrés Alfonso Gil Montenegro ◽  
Juan Sebastian Arocha Morales ◽  
Lilia Carolina Rojas Pérez ◽  
Paulo César Narváez Rincón
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dry Weight ◽  
Alkaline Pretreatment ◽  
Raw Material ◽  
Brewing Industry ◽  
Brewer’S Spent Grain ◽  
Spent Grain ◽  
Two Stages ◽  
Xylose Production ◽  
Hydrolysis Of

This work presents the simulation in Aspen Plusr of a process to obtain arabinoxylans (AX) from Brewer’s Spent Grain (BSG), which is the major byproduct of the brewing industry. The process is divided into two stages: alkaline pretreatment and enzymatic hydrolysis. These stages cover the extraction of proteins and AX from BSG using an alkaline pretreatment and enzymatic hydrolysis of the AX separated from the liquid stream to obtain xylose, i.e. the substrate required for the fermentation to xylitol. Simulation results show that xylose obtained corresponds to 8,5% of the dry weight of the raw material, obtaining a yield of 58%. Several streams of byproducts were obtained, such as proteins, polypeptides, amino acids, phenolic compounds and lignocellulosic residues that can be valorized in other processes. Simulation was performed in the context of a biorefinery in Colombia.

scholarly journals Enzymatic Hydrolysis of Lignocellulose for Bioethanol Production

2017 ◽  
Vol 71 (4) ◽  
pp. 275-279
Author(s):  
Linda Rozenfelde ◽  
Māris Puķe ◽  
Irēna Krūma ◽  
Ieva Poppele ◽  
Nataļja Matjuškova ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Effect Of Temperature ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Different Temperatures ◽  
C 50 ◽  
Hydrolysis Of ◽  
Temperature Time ◽  
Furfural Production

Abstract The effect of temperature, time and amount of enzyme on hydrolysis of wheat straw lignocellulose remaining after furfural production was studied. The residual substrate was subjected to enzymatic hydrolysis at different temperatures — 45 °C, 50 °C and 55 °C. Hydrolysis time was 72 hours, and samples were taken every 24 hours. The maximum glucose yield (76.5% of the theoretically possible) was reached when hydrolysis temperature 50 °C was used. The production rate of glucose increased with a hydrolysis period of time. The yield of glucose significantly depended on the ratio of enzyme to substrate.

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