scholarly journals Biosynthesis of Zinc Oxide (ZnO) Using Culture Biomass of Aspergillus Niger : The Influence of pH On Textile Morphology And Antimicrobial Activity

2021 ◽  
Author(s):  
Yetria Rilda ◽  
Rifki Rinaldi ◽  
Syukri Syukri ◽  
Armaini Armaini ◽  
Refinel Refinel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Aspergillus Niger ◽  
Sol Gel ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Hydroxyl Groups ◽  
Cell Biomass ◽  
A Value ◽  
Size And Morphology

Abstract The biosynthesis of zinc oxide (ZnO) is performed through enzymatic mechanisms, by controlling the particle size and morphology of the compound using the sol-gel method. Furthermore, this method utilizes stabilizer obtained from the cell biomass of Aspergillus niger bacteria, in order to yield homogeneous and consistent products, which corresponds with the XRD outcome that exhibits good crystallinity. The evaluation of functional, morphological, and antibacterial activities was carried out at pH 6.0-13.0, as the prepared samples were characterized by FT-IR. The analysis showed the interaction of hydroxyl groups, aromatic rings, as well as N-H and O-Zn-O compounds at a wavenumber of 401-584 cm−1. The XRD and SEM characterizations showed that the structure and crystal phase of ZnO were hexagonal wurtzite at dimensions of 36.2 - 45.4 nm. The differences in pH also influenced the dimensions, morphology, and antimicrobial activity. ZnO with pH of 8.0 was characterized by FESEM-EDAX, based on the analysis of morphological uniformity. This characterization obtained rod and cube structures, with atomic ratios of Zn = 61.5% and O = 38.5%. The UV-DRS spectrum showed that the optical band gap was not significantly influenced by the pH of the reactant solution, with a value of Eg = 3.00–3.11 eV. The differences in morphology further distinguished anti-bacterial properties on textile fibres, through the use of the Gram-negative and positive bacteria (Pseudomonas aeruginosa and Staphylococcus aureus), with inhibition zones of 17-21 mm and 21-25 mm, respectively. Therefore, ZnO is classified as a very strong antibacterial material compared to amoxicillin, with an inhibition zone of 13.6 mm.

scholarly journals Biosynthesis of Zinc Oxide (ZnO) Using Culture Biomass of Aspergillus Niger :The Influence of pH On Textile Morphology And Antimicrobial Activity

2021 ◽  
Author(s):  
Yetria Rilda ◽  
Rifki Rinaldi ◽  
Syukri Syukri ◽  
Armaini Armaini ◽  
Refinel Refinel ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Aspergillus Niger ◽  
Sol Gel ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Hydroxyl Groups ◽  
Cell Biomass ◽  
A Value ◽  
Size And Morphology

Abstract The biosynthesis of zinc oxide (ZnO) is performed through enzymatic mechanisms, by controlling the particle size and morphology of the compound using the sol-gel method. Furthermore, this method utilizes stabilizer obtained from the cell biomass of Aspergillus niger bacteria, in order to yield homogeneous and consistent products, which corresponds with the XRD outcome that exhibits good crystallinity. The evaluation of functional, morphological, and antibacterial activities was carried out at pH 6.0-13.0, as the prepared samples were characterized by FT-IR. The analysis showed the interaction of hydroxyl groups, aromatic rings, as well as N-H and O-Zn-O compounds at a wavenumber of 401-584 cm−1. The XRD and SEM characterizations showed that the structure and crystal phase of ZnO were hexagonal wurtzite at dimensions of 36.2 - 45.4 nm. The differences in pH also influenced the dimensions, morphology, and antimicrobial activity. ZnO with pH of 8.0 was characterized by FESEM-EDAX, based on the analysis of morphological uniformity. This characterization obtained rod and cube structures, with atomic ratios of Zn = 61.5% and O = 38.5%. The UV-DRS spectrum showed that the optical band gap was not significantly influenced by the pH of the reactant solution, with a value of Eg = 3.00–3.11 eV. The differences in morphology further distinguished anti-bacterial properties on textile fibres, through the use of the Gram-negative and positive bacteria (Pseudomonas aeruginosa and Staphylococcus aureus), with inhibition zones of 17-21 mm and 21-25 mm, respectively. Therefore, ZnO is classified as a very strong antibacterial material compared to amoxicillin, with an inhibition zone of 13.6 mm.

Antibacterial Activities of Zinc Oxide Nanostructures with Different Structures

2019 ◽  
Vol 294 ◽  
pp. 36-41
Author(s):  
Rolen Brian P. Rivera ◽  
Melchor J. Potestas ◽  
Ma. Reina Suzette B. Madamba ◽  
Rey Y. Capangpangan ◽  
Bernabe L. Linog ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Sea Urchin ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
The Other ◽  
Zno Nanostructures ◽  
Zinc Oxide Nanostructures ◽  
Sem Images ◽  
Structural Morphology ◽  
Oxide Nanostructures

We report on antibacterial activities of Zinc oxide (ZnO) with different structures. Fast furrier transform infrared spectroscopy ZnO nanostructures showed peaks in the range between 450–600 cm-1 indicating the successful growth through the presence of Zn-O stretching. On the other hand, impurities such as zinc complexes might be present due to the appearance of peaks at 1110 cm-1, 1390 cm-1 and 1506 cm-1. Furthermore, SEM images revealed that nanorods and sea-urchin like nanostructures are present in the produced ZnO nanostructures. Nanorods exhibit a better antibacterial response than the sea-urchin like structure. The change in structural morphology along with its purity has greatly influenced the area of bacterial inhibition zone during antibacterial testing.

Antifungal and Antibacterial Activities of Pentanema divaricatum and Its Active Constituent

2008 ◽  
Vol 63 (9-10) ◽  
pp. 649-652 ◽  
Author(s):  
Nastaran Momen-Roknabadi ◽  
Ahamd R. Gohari ◽  
Hamid R. Monsef-Esfehani ◽  
Farideh Attar ◽  
Reza Hajiaghaee ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Preparative Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Active Constituent ◽  
Disk Diffusion Method ◽  
Bioassay Guided Fractionation ◽  
Layer Chromatography ◽  
And Staphylococcus Aureus

The antimicrobial activity of ethanol and chloroform extracts of Pentanema divaricatum Cass. was studied using the conventional disk diffusion method. The extracts’ highest antimicrobial activity was observed against Aspergillus niger. Bioassay-guided fractionation of the crude extract by preparative thin layer chromatography (PTLC) showed one antimicrobial fraction which was especially effective against Aspergillus niger. By conventional spectroscopy the active fraction was identified as 4α,5α-epoxy-10α,14H-1-epi-inuviscolide. This compound represented the most potent antimicrobial candidate, with MIC values of < 25 μg/disk against A. niger strains and 200 μg/disk against Bacillus cereus and Staphylococcus aureus.

scholarly journals Effect of Cationic Surfactants on Properties of Zinc Oxide Nanoparticles Synthesized through Sol-gel Technique

2019 ◽  
Vol 3 (2) ◽  
pp. 93
Author(s):  
Hwda Ghafur Rauf ◽  
Hamid Rashidi Nodeh ◽  
Madzlan Aziz ◽  
Sattar Ibrahim Kareem
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cationic Surfactants ◽  
Near Infrared ◽  
Sol Gel ◽  
Hexagonal Wurtzite Structure ◽  
Band Gap Energy ◽  
Chemical Production ◽  
X Ray ◽  
Size And Morphology

Zinc oxide (ZnO) is an important metal oxide which participates in lots of applications, including gas sensors, catalysis, and optoelectronic devices. For nanostructured materials, agglomeration is problematic in the chemical production processes. In surfactant-free chemical reactions, aggregation occurs immediately as particles are generated. Therefore, the synthesis of controllable size nanoparticles is of great fundamental and technological interest. In the present study, the effect of cationic surfactants such as cetyltrimethylammonium bromide and benzalkonium chloride concentrations, on the optical properties, size, and morphology of ZnO nanoparticles synthesized through sol-gel method were studied. The characterizing tools were X-ray diffraction, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and ultraviolet-visible (UV-Vis-near-infrared) spectrophotometer. X-ray diffractogram confirmed the hexagonal (wurtzite structure) of ZnO. FTIR analyzed the presence of functional groups. The shape and size of the ZnO nanostructures were revealed by SEM and TEM. The band gap energy of the prepared ZnO samples was determined by UV-Vis spectrophotometer.

scholarly journals Application of Response Surface Methodology for Optimizing the Therapeutic Activity of ZnO Nanoparticles Biosynthesized from Aspergillus niger

Biomimetics ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 34
Author(s):  
Ali Es-haghi ◽  
Mohammad Ehsan Taghavizadeh Yazdi ◽  
Mohammad Sharifalhoseini ◽  
Mohsen Baghani ◽  
Ehsan Yousefi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Response Surface Methodology ◽  
Aspergillus Niger ◽  
Response Surface ◽  
Inhibition Zone ◽  
Diffraction Field ◽  
Contour Plot ◽  
Scale Production ◽  
Zno Nps

In this study, the biosynthesis of zinc oxide nanoparticles using Aspergillus niger (A/ZnO-NPs) is described. These particles have been characterized by UV–Vis spectrum analysis, x-ray powder diffraction, field emission scanning electron microscopy, and transmission electron microscopy. To use this biosynthesized nanoparticle as an antiproliferative and antimicrobial agent, the IC50 value against the breast cancer cell line and inhibition zone against Escherichia coli were used to optimize the effect of two processing factors including dose of filtrate fungi cell and temperature. The biosynthesized A/ZnO-NPs had an absorbance band at 320 nm and spherical shapes. The mean particles size was 35 nm. RSM (response surface methodology) was utilized to investigate the outcome responses. The Model F-value of 12.21 and 7.29 implies that the model was significant for both responses. The contour plot against inhibition zone for temperature and dose showed that if the dose increases from 3.8 to 17.2 µg/mL, the inhibition zone increases up to 35 mm. As an alternative to chemical and/or physical methods, biosynthesizing zinc oxide NPs through fungi extracts can serve as a more facile and eco-friendly strategy. Additionally, for optimization of the processes, the outcome responses in the biomedical available test can be used in the synthesis of ZnO-NPs that are utilized for large-scale production in various medical applications.

scholarly journals SCREENING OF ANTIMICROBIAL ACTIVITY IN PENTATROPIS MICROPHYLLA (ROTH) WIGHT (APOCYNACEAE)

2017 ◽  
Vol 4 (2) ◽  
pp. 127-129
Author(s):  
Prema R ◽  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method

The stem bark extracts of the medicinal plant species, Pentatropis microphylla Roth Wight by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against four human pathogenic bacteria viz., Bacillus subtilis, B. thuriengensis, Klebsiella pneumoniae and Escherichia coli and fourhuman pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii and Fusarium oxysporum for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (17.23 mm) against the bacterium, Klebsiella pneumoniae and the fungus, Aspergillus niger (19.63 mm). Therefore the result strengthens the existing traditional usage of the plant for the therapeutic use.

scholarly journals Effects of Different TiO2 Nanoparticles Concentrations on the Physical and Antibacterial Activities of Chitosan-Based Coating Film

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1365 ◽  
Author(s):  
Yage Xing ◽  
Xuanlin Li ◽  
Xunlian Guo ◽  
Wenxiu Li ◽  
Jianwen Chen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Tio2 Nanoparticles ◽  
Fruits And Vegetables ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Coating Film ◽  
Coating Films ◽  
Tio2 Nps ◽  
Chitosan Matrix

In this investigation, the effect of different concentrations of titanium dioxide (TiO2) nanoparticles (NPs) on the structure and antimicrobial activity of chitosan-based coating films was examined. Analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) revealed that the modified TiO2 NPs were successfully dispersed into the chitosan matrix, and that the roughness of the chitosan-TiO2 nanocomposites were significantly reduced. Moreover, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses indicated that the chitosan interacted with TiO2 NPs and possessed good compatibility, while a thermogravimetric analysis (TGA) of the thermal properties showed that the chitosan-TiO2 nanocomposites with 0.05% TiO2 NPs concentration had the best thermal stability. The chitosan-TiO2 nanocomposite exhibited an inhibitory effect on the growth of Escherichia coli and Staphylococcus aureus. This antimicrobial activity of the chitosan-TiO2 nanocomposites had an inhibition zone ranging from 9.86 ± 0.90 to 13.55 ± 0.35 (mm). These results, therefore, indicate that chitosan-based coating films incorporated with TiO2 NPs might become a potential packaging system for prolonging the shelf-life of fruits and vegetables.

scholarly journals Antimicrobial activity of root canal sealers

2006 ◽  
Vol 53 (2) ◽  
pp. 104-112 ◽  
Author(s):  
Mirjana Vujaskovic ◽  
Branka Radosavljevic
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Epoxy Resin ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Glass Ionomer ◽  
Zinc Oxide Eugenol ◽  
Root Canal Sealers

All root canal sealers should have antimicrobial activity so that they could act against any remaining bacteria after root canal obturation. The aim of this study was to evaluate the antimicrobial activity of five root canal sealers: AH26, a resin based paste; Apexit, calcium hydroxide based paste; Endomethasone and Tubliseal, zinc oxide eugenol based materials and Ketac Endo Aplicap, glass ionomer based sealer. Matherials And Methods: Antimicrobial activity was tested against S. mutans 70C and L.casei ATCC 27773 using ADT (agar diffusion inhibitory test) on TYC SB, blood and MRS agars. Freshly prepared paste or cement was placed in grooves of 4 mm in diameter in agar plates. Prepared agar plates were incubated at 37?C in GAS PAC system for 24hours. The antimicrobial effect was determined according to the diameter of growth inhibition zone around tested materials. Three measurements were done for each material and values were recorded in millimeters. Statistical analysis was done using Mann Whitney test. The results confirmed that epoxy resin and zinc oxide eugenol based sealers had the greatest antimicrobial effect. Calcium hydroxide and glass ionomer based sealers showed significantly lower antimicrobial activity compared to AH26, Endomethasone and Tubliseal. Conclusion: The greatest antimicrobial activity was found for epoxy resin based sealer (AH26) for both tested microorganisms.

In Situ Growth of Zinc Oxide Nanoparticles on Cotton Fabric Using Sonochemical Method

2013 ◽  
Vol 856 ◽  
pp. 53-59 ◽  
Author(s):  
Leila Zarei ◽  
Sheila Shahidi ◽  
Seyed Mohammad Elahi ◽  
Arash Boochani
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Woven Fabric ◽  
Semiconductor Materials ◽  
Sonochemical Method ◽  
Morphological Transformation ◽  
Zno Nps ◽  
Size And Morphology

Researches show that properties of semiconductor materials such as electrical, optical and antibacterial properties can be improved in the nanoscale. Among the semiconductor materials , zinc oxide with excellent electronic properties has been considered extremely. Application and properties of Zno nanoparticles depends on the size and morphology. In the present work, Zno Nanoparticles (NPs) were prepared by sonochemical method at a constant temperature and were applied on to the 100% cotton woven fabric using Insitu method. The results show that nanoparticles with average sizes of 20-100 nm with different morphologies were created on the surface of samples. Synthesis of ZnO-NPs were varied in the morphological transformation by changes in zinc acetate concentration. Characterizations were carried out, Scanning electron microscopy (SEM) and Spectrophotometery. The antibacterial activities of the fabrics were assessed semi-quantitatively by the coloneis count method. The results show that the finished fabric demonstrated significant antibacterial activity against S.aureus in antibacterial test.

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