scholarly journals Establishment of a Standard Micropropagation Protocol for the Production of Uniform Planting Material in Musa Laterita (Section: Rhodochlamys).

2021 ◽  
Author(s):  
Saraswathi. M.S. ◽  
J Mahendran ◽  
Chinnadurai Karthi ◽  
Murugesan Umabharathi ◽  
Arumugam Palanivelu Salini ◽  
...  
Keyword(s):  
Fusarium Wilt ◽  
Minimum Duration ◽  
Planting Material ◽  
Moisture Deficit ◽  
Micro Propagation ◽  
Banana Industry ◽  
Musa Species ◽  
Shoot Tip Explants ◽  
Moisture Deficit Stress

Abstract Musa laterita is one of the dwarf statured, ornamental Musa species, which belongs to the section Rhodochlamys. It is immune to Sigatoka leaf spot and Fusarium wilt diseases and also exhibits tolerance to moisture deficit stress. Among the fungal diseases, fusarium wilt especially race TR4 is highly threatening the banana industry worldwide and hence the TR4 resistant gene should be identified on priority for use in banana improvement programmes. Though, it is a wild seeded type which can be easily propagated through seeds, the off springs are not genetically uniform making it unfit for any molecular analysis. In vitro micro propagation of ornamental banana including Musa laterita is limited due to poor multiplication rates compared to commercial cultivars. Therefore, the present study was undertaken to establish an in vitro protocol for plant regeneration from shoot tip explants. Efforts were made to enhance the multiplication efficiency using MS media fortified with BAP, TDZ, NAA, IAA and IBA in different combinations so as to obtain maximum numbers of plantlets in minimum duration. The plantlets produced would be used to study the molecular mechanism behind Fusarium wilt resistance (TR4), through transcriptome analysis.

scholarly journals Effect of different growth regulators on in vitro micro-propagation of Kufri Frysona

2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Basal Medium ◽  
Potato Cultivar ◽  
Shoot Tips ◽  
Scale Production ◽  
Survival Percentage ◽  
Planting Material ◽  
Micro Propagation

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.

scholarly journals ​Effect of Different Media on In vitro Rooting in Different Cultivar of Lilium longiflorum Cv. Elite, Brunello, Cordelia

2021 ◽  
Author(s):  
Manoj Kundu ◽  
Suresh Kumar ◽  
Rajesh Lathar ◽  
Sakshi .
Keyword(s):  
Tissue Culture ◽  
Cost Effective ◽  
Lilium Longiflorum ◽  
Global Market ◽  
Root Regeneration ◽  
Planting Material ◽  
Micro Propagation ◽  
The Media ◽  
Increasing Demand

Background: Lilium (Lilium longiflorum Thunb.) belongs to the family Liliaceae and is a native of Northern Hemisphere (up to South Canada and Siberia). Conventionally Lilium can easily be propagated by sexual and asexual methods of propagation but these prevalent methods are not capable of meeting the increasing demand in domestic and global market. Generally, Lilium is propagated through bulbs but, limited number of bulbs per plant, long dormancy period of bulbs which again results into non-availability of planting material throughout the year. Keeping in view the above facts, the present study was undertaken with the following objective: “To standardize the cost effective protocol for micro propagation of lilium to produce disease free and true to type plants at a faster rate”. Methods: The present investigation was carried out in the Tissue Culture Laboratory of the Centre for Research and Application in Plant Tissue Culture. The experiment was laid out in a C.R.D. (Factorial) with three replications. In vitro raised bulblets were separated out and were transferred on to the root regeneration media. Different levels of NAA were used in MS media for the rooting of in vitro raised bulblets and percent rooting of plantlet is recorded. Result: It was interesting to note that the media LR-3 (MS + NAA 1.0 mg/l) is most efficient for rooting in all type of cultivars. All the three cultivars used responded very poor on media LR-1 (MS basal).

In vitro propagation of Althaea rosea - a valuable medicinal plant of Kashmir Himalaya

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Sumira Tyub ◽  
Azra N. Kamili ◽  
Mohammad Mansoor Bhat
Keyword(s):  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Food Industry ◽  
Shoot Tip ◽  
Kashmir Himalaya ◽  
Plant Product ◽  
Shoot Number ◽  
Micro Propagation ◽  
Shoot Tip Explants

Althaea rosea, commonly known as Hollyhock is an ornamental and medicinal plant. A. rosea has been reported to contain highest amount of tannins, carbohydrates, cyanide and mucilage. The purpose of this study was to optimize conditions for multiplication of Althaea rosea using shoottip explants. Varying concentrations solo and in combinations of auxins and cytokinins were experimented. The development of micro-propagation protocol for this valuable medicinal plant can ensure continuous supply of the plant product for pharmaceutical and food industry. In this regard an efficient and reproducible micro-propagation protocol has been developed using Murashige and Skoog’s (MS) (1962) medium augmented with different phytohormonal combinations like BAP, NAA, IBA and IAA. Both direct and indirect multiplication was obtained using in vitro raised shoot tip explants. Maximum shoot number was obtained using combination of BAP and NAA and rooting of isolated shoots was obtained using IBA. 60% of the transplanted plantlets survived under laboratory conditions.

scholarly journals Technologies of Reproduction Terry Varieties of Clematis

2020 ◽  
Vol 12 (3) ◽  
pp. 214
Author(s):  
Khanbabaeva Olga Evgenievna ◽  
Matsneva Anna Evgenievna ◽  
Kovaleva Irina Sergeevna ◽  
Tazina Svetlana Vitalevna ◽  
Tazin Ivan Ivanovich
Keyword(s):  
In Vitro Propagation ◽  
Agricultural Technology ◽  
High Yield ◽  
Innovative Technology ◽  
Flower Buds ◽  
Planting Material ◽  
Breeding Technology ◽  
Other Substances ◽  
Micro Propagation

The paper presents issues of optimizing the technology of growing popular terry varieties of clematis (Clematis L.) in Russia. Trimming groups, bookmark features of flower buds are described. The issues of reproduction and agricultural technology when laying uterine plantations are considered. A comparative analysis of traditional breeding technology with innovative technology (in vitro). The optimal nutrient medium for the stages of micro propagation, cultivation and rooting, enriched with vitamins and other substances. In experiments on green cuttings, 8 terry varieties were used. Estimated rooting of green cuttings by standard propagation technology. Spring cuttings are recommended for the varieties Bellof Woking (83%), Empress (81%), Blue Light (79%) and give a high yield of planting material from one mother plant. Two varieties with low rooting were selected—Purpurea Plena Elegans, Multi Blue. In vitro breeding technology has been developed for them. Accounting and observation of the development of two terry clematis varieties in the tissue culture: Purpurea Plena Elegans, Multi Blue. The aim of our study is to develop a technology for clonally propagation for these varieties, as well as the adaptation of micro plants of these varieties to non-sterile conditions. Therefore, in vitro propagation is recommended for these two varieties. An optimal substrate composition has been developed for the adaptation of clematis plants propagated in vitro, as well as for cuttings. The article provides recommendations for planting, pruning and caring for uterine plantings of clematis with double flowers in sheltered ground.

scholarly journals Ipomoea batatas (L.) Lam. cultivation in the conditions of light culture in vitro and ex vitro

2021 ◽  
pp. 22-29
Author(s):  
E. A. Kalashnikova ◽  
R. N. Kirakosyan ◽  
A. V. Gushchin ◽  
K. G. Abubakarov ◽  
N. N. Sleptsov ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Human Life ◽  
Red Light ◽  
The Body ◽  
Ex Vitro ◽  
High Quality ◽  
Planting Material ◽  
Micro Propagation

Relevance. Currently, food products that include prebiotics, in particular, inulin, are particularly popular. Interest in this substance is justified by its valuable properties – it is a good immunomodulator, cleanses the body of toxins, radionuclides, "bad" cholesterol, promotes the assimilation of useful trace elements necessary for human life. Inulin is contained in plants such as jerusalem artichoke, chicory, as well as in sweet potatoes, the popularity of which is increasing every year. However, sweet potato plants are afraid of cold and frost-resistant. Therefore, the creation of new varieties and hybrids that are resistant to low temperatures is an urgent problem. Cellular biotechnology is aimed at solving this problem using methods of clonal microreproduction, cell selection, somatic hybridization, etc. For rapid reproduction and obtaining high-quality planting material, biotechnology methods are used, in particular, clonal micro-propagation. However, in this technology there are difficulties associated with poor adaptation of microclones to ex vitro conditions. This fact introduces an additional requirement for the selection of optimal rooting modes in vitro and ex vitro adaptation of microclones.Material and methodology. The aim of the work was to study the influence of cultivation conditions on in vitro rooting and ex vitro adaptation of I. batatas (L.) microclones. The object of the study was sweet potato microgears propagated in vitro. I. batatas micro-gears were cultured in vitro on a Murashige-Skug medium, differing by the type of auxins. The influence of red (R) and far red (FR) light on the shoots rooting in vitro and the adaptation of microclones ex vitro was studied.Results. It has been experimentally established that the cultivation of micro-gears on a medium containing indolyl butyric acid at a concentration of 0.5-1 mg/l and under conditions of illumination by LED lamps of red and far red light in equal amounts leads to the production of microclones with a well-developed root system and vegetative biomass. The use of an aeroponic installation at the last stage of clonal micro-propagation makes it possible to obtain high-quality planting material that can adapt well to open ground conditions.

In vitro screening of Vigna mungo genotypes for PEG induced moisture deficit stress

2013 ◽  
Vol 18 (1) ◽  
pp. 55-60
Author(s):  
S. K. Yadav ◽  
N. Jyothi Lakshmi ◽  
Vikram Singh ◽  
Amol Patil ◽  
Yogesh Kumar Tiwari ◽  
...  
Keyword(s):  
Vigna Mungo ◽  
In Vitro Screening ◽  
Moisture Deficit ◽  
Moisture Deficit Stress

scholarly journals In vitro selection for Fusarium oxysporum f. sp. conglutinans resistance in brassica vegetables

2019 ◽  
Vol 5 (2) ◽  
pp. 35
Author(s):  
Oushadee A. J. Abeyawardana ◽  
Martin Koudela
Keyword(s):  
Fusarium Wilt ◽  
Culture Filtrate ◽  
Shoot Tip ◽  
Chemical Mutagenesis ◽  
Induction Medium ◽  
Shoot Induction ◽  
Development Of Resistance ◽  
Shoot Induction Medium ◽  
Shoot Tip Explants

<p class="abstract"><strong>Background:</strong> Fusarium wilt is an issue of concern in economically and nutritionally important Brassica vegetable cultivation. Thus, it deserves measures against the adverse production impact caused by Fusarium wilt.</p><p class="abstract"><strong>Methods:</strong> In this study, development of resistance to <em>F. oxysporum</em> f. sp. <em>conglutinans</em> in six white head cabbage cultivars, by in vitro chemical mutagenesis and selection, through direct and indirect organogenesis was examined. 6 day and 10 day old hypocotyl, shoot tip and calli, from 6 day old hypocotyl explants, were subjected to chemical mutagenesis treatment (DMSO (4% v/v) + EMS (0.3% v/v) for two hours at 28±2°C) were incubated in MS shoot induction medium (MS+ NAA (0.2 mg/l), BAP (3 mg/l), GA3 (0.01 mg/L) and AgNO3 (0.5 mg/l)). Shoots developed from hypocotyl and shoot tip explants (in the MS shoot induction medium and then in MS + NAA (0.2 mg/l), BAP (3 mg/l), GA3 (0.01 mg/l) for shoot development) and calli following mutagenesis treatment were screened for Fusarium resistance subjecting to 15% and 20% <em>Fusarium</em> culture filtrate for 30 and 60 day selection periods for each strength respectively.  </p><p class="abstract"><strong>Results:</strong> Developed plantlets from all six cultivars tested, showed resistance to<em> </em>Fusarium culture filtrate in the in vitro conditions with different survival frequencies ranging between 12.5% to 84.0% from hypocotyl and 0.0% to 86.7% from shoot tip explants among cultivars indicating development of resistance to <em>Fusarium</em> by in vitro chemical mutagenesis.</p><p class="abstract"><strong>Conclusions:</strong> Direct organogenesis, and 10 day old hypocotyl and 6 day old shoot tips are potential explants for successful application of in vitro chemical mutagenesis for Fusarrium resistance development in in cabbage.</p><p class="abstract"> </p>

scholarly journals Garden Strawberry Plants: From Test-Tubes to Plantations

2019 ◽  
Author(s):  
A A Batukaev ◽  
S A Kornatskiy
Keyword(s):  
Crucial Role ◽  
In Vitro Propagation ◽  
Production Efficiency ◽  
Soil Substrate ◽  
Planting Material ◽  
Micro Propagation ◽  
Technical Solutions

The article discusses the role of strawberry in providing people with vitamin products. It emphasizes the direct dependence of productivity of strawberry plants on the quality of the original planting material. A crucial role of clonal micro-propagation was justified. Features of the in vitro propagation stages were identified and new technical solutions for improving production efficiency were offered. The efficiency of auxin-containing paste for adaptation to the peat-based soil substrate was proved. The use of a 2 % Diazinon solution killed sciaride larvae damaging the roots. Development of an alternative method for adapting microplants to hydroponics made it possible to abandon the use of any substrate and ensure the viability of plants at the level of 95–100 %.

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