scholarly journals Comparative Physiological and Transcriptomic Analysis Reveal MdWRKY75 Associated With Sucrose Accumulation in Postharvest Apples With Bitter Pits

2021 ◽  
Author(s):  
Chen Sun ◽  
Weiwei Zhang ◽  
Haiyong Qu ◽  
Longfei Yan ◽  
Lixia Li ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Principal Component ◽  
Calcium Deficiency ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Sucrose Accumulation ◽  
Ca Deficiency ◽  
Transient Overexpression ◽  
Underlying Mechanisms

Abstract Background: Calcium (Ca) deficiency can cause apple bitter pits, reduce the quality and shelf life. WRKY Transcription factors play essential role in plant response to multiple diseases. However, the underlying mechanisms causing bitter pits in apple fruit due to Ca deficiency during storage is extremely limited. Results: In the present study, the nutritional metabolites and reactive oxygen species (ROS) were compared in Ca-deficient and healthy apple fruit (CK) during storage. Results showed that Ca-deficient apples sustained significantly higher production of ROS, PPO activity, flavonoids, total phenol, total soluble solids (TSS), and sucrose contents, but the contents of Ca, H2O2, titratable acids (TA), glucose and fructose were significantly lower than those of CK during storage. Principal component analysis (PCA) showed that TSS, •O2−­, PPO, MDA and Ca were the main factors, and TSS had a positive correlation with sucrose. Furthermore, transcriptome analysis revealed that WRKYs were co-expressed with sucrose metabolism-related enzymes (SWEETs, SS, SPS). RT-qPCR and correlation analysis indicated that MdWRKY75 were significantly positively correlated with MdSWEET1. Moreover, transient overexpression of MdWRKY75 could significantly increase the sucrose content and promote the expression of MdSWEET1 in apple fruit.Conclusions: Calcium deficiency could decrease antioxidant capacity, accelerate nutritional metabolism and up-regulate the expression of WRKYs in apple with bitter pits. Overexpression of MdWRKY75 significantly increased sucrose accumulation and the expression of MdSWEET1. These findings further strengthened knowledge of the basic molecular mechanisms in calcium-deficient apple flesh and contributed to improving the nutritional quality of apple fruit.

scholarly journals Silicon attenuates calcium deficiency by increasing ascorbic acid content, growth and quality of cabbage leaves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dalila Lopes da Silva ◽  
Renato de Mello Prado ◽  
Luis Felipe Lata Tenesaca ◽  
José Lucas Farias da Silva ◽  
Ben-Hur Mattiuz
Keyword(s):  
Ascorbic Acid ◽  
Nutrient Solution ◽  
Acid Content ◽  
Dry Matter ◽  
Ascorbic Acid Content ◽  
Calcium Deficiency ◽  
Ca Deficiency ◽  
Leaf Water Loss ◽  
Nutrient Solutions

AbstractCalcium (Ca) deficiency in cabbage plants induces oxidative damage, hampering growth and decreasing quality, however, it is hypothesized that silicon (Si) added to the nutrient solution may alleviate crop losses. Therefore, this study aims at evaluating whether silicon supplied in the nutrient solution reduces, in fact, the calcium deficiency effects on cabbage plants. In a greenhouse, cabbage plants were grown using nutrient solutions with Ca sufficiency and Ca deficiency (5 mM) without and with added silicon (2.5 mM), arranged as a 2 × 2 factorial in randomized blocks, with five replications. At 91 days after transplanting, the plants were harvested for biological evaluations. In the treatment without added Si, Ca deficiency promoted oxidative stress, low antioxidant content, decreased dry matter, and lower quality leaf. On the other hand, added Si attenuated Ca deficiency in cabbage by decreasing cell extravasation while increasing both ascorbic acid content and fresh and dry matter, providing firmer leaves due to diminished leaf water loss after harvesting. We highlighted the agronomic importance of Si added to the nutrient solution, especially in crops at risk of Ca deficiency.

scholarly journals Influence of plant immunity inducers on the quality of apple fruit in Abkhazia

2020 ◽  
Vol 21 ◽  
pp. 00026
Author(s):  
Oksana Belous ◽  
Georgiy Pantiya ◽  
Yelena Mikhailova ◽  
Natalia Karpun ◽  
Liosik Ayba
Keyword(s):  
Plant Immunity ◽  
Soluble Sugars ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Tree Protection ◽  
Urgent Task ◽  
Apple Cultivars ◽  
The Republic ◽  
Selection Of

The quality of fruit depends largely on the growing area of the crop, the intensity of disease development, damage by pests, as well as on the treatment of plants with compounds of various chemical natures. When introducing the immunity inducers Albit® (poly-beta-hydroxybutyric acid), Immunocytophyte® (arachidonic acid ethyl ether) and Ecogel® (chitosan lactate) into apple tree protection systems, an urgent task is to study their influence on the quality of fruits. Research was conducted on apple trees (cultivars Idared and Golden Rangers) in the conditions of the Republic of Abkhazia (Gulripsh district). When treated with immunity inducers, the genotypic features of the cultivars were clearly revealed. For the susceptible Idared cultivar, it is more significant to use Ecogel®, which causes the active synthesis of soluble solids, pectin, soluble sugars, ascorbic acid, and as a result, an increase in the sugar-acid index. At the same time, a relatively resistant cultivar Golden Rangers has a similar effect when treated with Immunocytophyte®. Treatment with Albit® has the same effect on the synthesis of soluble organic acids in the fruits of the studied apple cultivars, reducing their amount. The results obtained indicate the need for a cultivar-specific approach in the application and selection of plant immunity inducers.

scholarly journals Responses of Apples to Postharvest Jasmonate Treatments

1998 ◽  
Vol 123 (3) ◽  
pp. 421-425 ◽  
Author(s):  
Xuetong Fan ◽  
James P. Mattheis ◽  
John K. Fellman
Keyword(s):  
Color Change ◽  
Titratable Acidity ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Maximum Effect ◽  
Minimum Concentration ◽  
Color Changes ◽  
Promotive Effect ◽  
Solids Content

The effect of exogenous methyl jasmonate (MJ) and jasmonic acid (JA) compared with the effect of ethephon on surface color and quality of `Golden Delicious' and `Fuji' apples (Malus ×domestica Borkh.) was studied. Treatments were applied by dipping fruit in water solutions of JA, MJ, or ethephon or by exposing fruit to MJ vapors. Response to MJ vapor treatment depended on fruit developmental stage, with the maximum effect occurring as fruit began to produce ethylene. MJ promoted color changes more effectively than JA. The promotive effect of JA increased with JA concentration. A minimum concentration of 0.1 mmol·L-1 JA was needed to promote significant color change within 15 d at 20 °C. JA at 1 or 10 mmol·L-1 promoted color change more effectively than 0.35 or 3.5 mmol·L-1 ethephon. The magnitude of JA-promoted responses decreased at lower temperatures. Treatments with 10 mmol·L-1 JA or 3.5 mmol·L-1 ethephon were phytotoxic. Treatments using JA at 1 or 10 mmol·L-1 in water promoted loss of fruit titratable acidity compared to controls. Firmness and soluble solids content were relatively unresponsive to JA treatments. Based on these results, using JA and MJ to promote degreening of apple fruit with minimal loss of other quality attributes appears feasible.

scholarly journals Volatile Emissions and Quality of Bagged `Fuji' Apples

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 638f-639
Author(s):  
J.P. Mattheis ◽  
D.A. Buchanan ◽  
J.K. Fellman
Keyword(s):  
Fungal Pathogens ◽  
Titratable Acidity ◽  
Apple Fruit ◽  
Washington State ◽  
Soluble Solids ◽  
Refrigerated Storage ◽  
Volatile Emissions ◽  
Fruit Maturity ◽  
Solids Content

Enclosing apple fruit in bags during development is widely practiced in Japan. Bags create a barrier that reduces damage from insects and fungal pathogens as well as treatments to control these problems. Bags also reduce the incidence of sunburn and change fruit appearance by altering peel pigmentation composition, two features that have prompted northwestern United States producers to bag `Fuji' apples. Fruit maturity and quality of bagged and nonbagged Fuji apples grown in Washington state were evaluated at harvest and after refrigerated storage in air or controlled atmosphere. Bagged fruit had less watercore and lower ethylene production at harvest compared to non-bagged fruit with similar starch ratings. Bagged fruit had lower soluble solids content, titratable acidity and firmness at harvest and during storage. Emission of ester and alcohol volatiles was consistently lower for bagged fruit. Postharvest volatile emissions were negatively correlated with bagging duration during development. Bagged fruit had no incidence of a peel disorder with similarity to delayed sunscald.

scholarly journals Assessment of Water Quality for Aquaculture in Hau River, Mekong Delta, Vietnam Using Multivariate Statistical Analysis

Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3307
Author(s):  
Fridah Gacheri Mutea ◽  
Howard Kasigwa Nelson ◽  
Hoa Van Au ◽  
Truong Giang Huynh ◽  
Ut Ngoc Vu
Keyword(s):  
Water Quality ◽  
Principal Component ◽  
Quality Parameters ◽  
Point Sources ◽  
Water Quality Parameters ◽  
Soluble Solids ◽  
Accumulation Factor ◽  
Multivariate Statistical ◽  
Aquaculture Production

The deterioration signs of water quality in the Hau River are apparent. The present study analyzed the surface water quality of the Hau River using multivariate statistical techniques, including principal component analysis (PCA) and Cluster Analysis (CA). Eleven water quality parameters were analyzed at 19 different sites in An Giang and Can Tho Provinces for 12 months from January to December 2019. The findings show high levels of Biological Oxygen Demand (BOD), Total Soluble Solids (TSS), and total coliform, all year round. The PCA revealed that all the water quality parameters influenced the water quality of the Hau River, hence the relevance for water sample scrutiny. The dendrogram of similarity between sampling sites showed a maximum similarity of 95.6%. The Accumulation Factor (AF) trend showed that the concentrations/values of TSS, BOD, and phosphate (PO43−) in the downstream were 1.29, 1.53, and 1.52 times, respectively, greater than the upstream levels. Despite most of the parameters analyzed supporting aquaculture production, caution is needed in the regulation of pollution point sources to undertake sustainable aquaculture production.

scholarly journals Effect of gamma radiation and pasteurization on the shelf-life of juçara pulp (Euterpe edulis)

2018 ◽  
Vol 67 (4) ◽  
pp. 471-478
Author(s):  
Paula Porrelli Moreira da Silva ◽  
José Guilherme Prado Martin ◽  
Marta Helena Fillet Spoto
Keyword(s):  
Gamma Radiation ◽  
Shelf Life ◽  
Sensory Quality ◽  
Principal Component ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Acceptance Test ◽  
Palm Fruit ◽  
Euterpe Edulis

Several studies about juçara palm fruit (Euterpe edulis) have emphasized its high antioxidant capacity.  However, there is a lack of studies comparing the effects of conservation technologies on the physicochemical and sensory quality of juçara pulp.  This work aimed at evaluating the effects of gamma radiation (60Co) and pasteurization process on the physicochemical and sensory quality of juçara pulp.  The irradiated (2.5 ± 0.06 kGy) and pasteurized (80°C/5 minutes) pulps were stored at 6°C, 90% RH, for 30 days (3 periods of analysis) and were analyzed for their microbiological, physicochemical (titratable acidity, soluble solids content, color, phenolic compounds, anthocyanin content, polyphenol oxidase and peroxidase activity) and sensory parameters (acceptance test.  The physicochemical results were submitted to ANOVA for the ‘F’ test and the statistical difference of averages (P < 0.05) was determined by the Tukey’s test.  The results of the sensory analysis were submitted to Principal Component and Cluster Analysis.  The gamma radiation did not show satisfactory results in the preservation of juçara pulp since there was a significant decrease in total phenolics and anthocyanin content.  The pasteurized pulp maintained its physicochemical and sensory characteristics during storage.  Therefore, we recommend the pasteurization as a good conservation technique to this kind of product, which presented a shelf-life of around 15 days

scholarly journals Maturity and Quality of ‘Liberty’ Apple Fruit Under Integrated and Organic Fruit Production Systems Are Similar

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1382-1389 ◽  
Author(s):  
Gregory M. Peck ◽  
Ian A. Merwin ◽  
Christopher B. Watkins ◽  
Kathryn W. Chapman ◽  
Olga I. Padilla-Zakour
Keyword(s):  
Transition Period ◽  
Fruit Production ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Internal Quality ◽  
Total Phenolic ◽  
Double Blind ◽  
Ethylene Concentration ◽  
Organic Fruit

Maturity and quality of fruit harvested from an orchard of disease-resistant ‘Liberty’ apple (Malus ×domestica Borkh.) trees was investigated during and after the transition from conventional to integrated (IFP) and organic fruit production (OFP) systems. Over 4 years, internal ethylene concentration, starch pattern index, flesh firmness, soluble solids concentration (SSC), titratable acidity (TA), and percent of surface blush of fruit at harvest were not consistently different between fruit from IFP and OFP systems. Total phenolic content and antioxidant capacity of the fruit were also similar between treatments. IFP-grown fruit contained more potassium during the first 2 years and more calcium in all years than OFP-grown fruit. After fruit were stored in air at 0.5 °C for 9 weeks in 2007, OFP-grown apples were firmer and had higher SSC, TA, and SSC:TA ratios. In double-blind triangle taste tests, consumer panelists were able to discriminate between the fruit from each treatment, but in double-blind hedonic and intensity tests, panelists did not consistently rate one treatment more highly than the other. Overall, consumer panelists favorably rated internal quality of fruit grown under both IFP and OFP systems. In 2006, when weather and disease caused a high percentage of OFP-grown fruit to have cosmetic defects, the panelists rated the appearance of OFP-grown apples as less acceptable than the cleaner-looking IFP-grown apples. Our study of ‘Liberty’ apple fruit maturity and quality during a 4-year transition period from conventional to IFP and OFP systems showed that differences were small if present, whereas internal fruit quality was rarely different between systems.

scholarly journals ClusterMap: Comparing analyses across multiple Single Cell RNA-Seq profiles

2018 ◽  
Author(s):  
Xin Gao ◽  
Deqing Hu ◽  
Madelaine Gogol ◽  
Hua Li
Keyword(s):  
Single Cell ◽  
Molecular Mechanisms ◽  
Population Level ◽  
Marker Genes ◽  
Rna Seq ◽  
Matching Problem ◽  
Cut Method ◽  
Underlying Mechanisms

AbstractSingle cell RNA-Seq facilitates the characterization of cell type heterogeneity and developmental processes. Further study of single cell profiles across different conditions enables the understanding of biological processes and underlying mechanisms at the sub-population level. However, developing proper methodology to compare multiple scRNA-Seq datasets remains challenging. We have developed ClusterMap, a systematic method and workflow to facilitate the comparison of scRNA profiles across distinct biological contexts. Using hierarchical clustering of the marker genes of each sub-group, ClusterMap matches the sub-types of cells across different samples and provides “similarity” as a metric to quantify the quality of the match. We introduce a purity tree cut method designed specifically for this matching problem. We use Circos plot and regrouping method to visualize the results concisely. Furthermore, we propose a new metric “separability” to summarize sub-population changes among all sample pairs. In three case studies, we demonstrate that ClusterMap has the ability to offer us further insight into the different molecular mechanisms of cellular sub-populations across different conditions. ClusterMap is implemented in R and available at https://github.com/xgaoo/ClusterMap.

scholarly journals Sprayable 1-MCP Effects on Maturity and Quality of Apple Fruit at Harvest and After Storage: The New York Experience

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 978A-978 ◽  
Author(s):  
Chris B. Watkins ◽  
Jacqueline F. Nock ◽  
Tarek Wardeh
Keyword(s):  
New York ◽  
Ethylene Production ◽  
Storage Period ◽  
Apple Fruit ◽  
Soluble Solids ◽  
Quality Factors ◽  
Solids Concentration ◽  
Harvest Dates ◽  
Spray Timing

A sprayable formulation of 1-MCP (250 μL·L-1) and 1% oil adjuvant was applied to `McIntosh' and `Empire' apple trees 24, 14, and 7 days prior to anticipated optimum harvest dates (early, mid-, and late-spray timings, respectively), and fruit harvested sequentially over 2 to 3 weeks from this date. At harvest, internal ethylene concentrations (IEC), percentage of blush, starch indices, firmness, and soluble solids concentration (SSC) were measured, as well as ethylene production of fruit maintained for 7 days at 20 °C. Additional fruit were stored in air (0.5 °C) with or without postharvest 1-MCP treatment. Preharvest drop of `McIntosh' apples was also measured. Quality of these fruit was assessed at intervals for up to 4.5 months (`McIntosh') or 6 months (`Empire'). All spray timing resulted in marked delays of preharvest drop. For both cultivars, increases of IEC were inhibited or delayed by sprayable 1-MCP treatment, but effects on other maturity and quality factors were small. Ethylene production of treated fruit was lower than that of untreated fruit. The effects of sprayable 1-MCP on IEC and firmness were maintained during storage, but the longetivity of these effects was affected by cultivar, spray timing, and storage period. Postharvest application of 1-MCP further inhibited IEC and maintained firmness of the fruit during storage. These experiments show that sprayable 1-MCP may be a valuable tool to manipulate both pre- and postharvest responses of apple fruit. However, with the formulation used in these experiments, phytotoxicity, primarily as damage around lenticel areas, was observed at harvest indicating that further development of the formulation is necessary for industry use.

scholarly journals Effect of Vector Control and Foliar Nutrition on Quality of Orange Juice Affected by Huanglongbing: Chemical Analysis

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1100-1106 ◽  
Author(s):  
Elizabeth Baldwin ◽  
Jinhe Bai ◽  
Anne Plotto ◽  
John Manthey ◽  
Smita Raithore ◽  
...  
Keyword(s):  
Principal Component ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Good Separation ◽  
Foliar Nutrition ◽  
Valencia Orange ◽  
Liberibacter Asiaticus ◽  
Solids Content ◽  
Orange Trees

‘Valencia’ orange trees from groves with 90% infection by Candidatus liberibacter asiaticus (CLas), the presumed pathogen for citrus greening or huanglongbing (HLB) disease, were treated with insecticide (I), a nutritional spray (N), and insecticide plus nutritional spray (I + N). Controls (C) were not treated. Fruit were harvested in March to April, 2013, 2014, and 2015, juiced, and the juice was frozen for later chemical analyses. Titratable acidity (TA), soluble solids content (SSC), SSC/TA ratio, many volatiles, flavonoids, and limonoids showed differences because of season, whereas SSC, several volatiles (ethanol, cis-3 hexenol, α-terpinene, ethyl acetate, and acetone), flavonoids (narirutin, vicenin-2, diosmin, nobiletin, heptamethoxy flavone), and limonoids (nomilin and nomilinic acid glucoside) showed differences because of treatment. However, consistent patterns for chemical differences among seasons were not detected. TA tended to be higher in N and C the first two seasons and SSC/TA higher in I and I + N for all seasons (not significant for 2014). Bitter limonoids tended to be higher in I, N or I + N over the seasons. Principal Component Analysis showed that there was a good separation by season overall and for treatment in 2013. In 2014 and 2015, the insecticide treatments (either I or I + N) had the highest sugar and SSC/TA levels and lowest TA levels, although not always significant, as well as higher juice CLas cycle threshold (Ct) levels, indicating lower levels of the pathogen.

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