scholarly journals In Vitro Propagation of the Rare and Endangered Grevillea scapigera (Proteaceae)

HortScience ◽  
1992 ◽  
Vol 27 (3) ◽  
pp. 261-262 ◽  
Author(s):  
Eric Bunn ◽  
Kingsley W. Dixon
Keyword(s):  
Shoot Growth ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Zeatin Riboside ◽  
Multiplication Rate ◽  
Rare And Endangered Species ◽  
Isopentenyl Adenine ◽  
Rare And Endangered

Micropropagation, including adventitious shoot growth from leaf sections, was achieved for Grevillea scapigera (Proteaceae), a rare and endangered species from Western Australia. Shoot tips were initiated on filter paper supports with liquid WPM (Woody Plant Medium) and supplemented with 20 μm zeatin riboside and 2 μm GA3. Shoots were then incubated on WPM solidified with agar and supplemented with 5 μm kinetin and 0.5 μm BA, which produced an approximate 6-fold multiplication rate per month. Up to three adventitious shoots were induced from 0.7-cm2 leaf sections after 6 to 7 weeks on solid 1/2 MS (Murashige and Skoog) medium supplemented with 10 μm BA and 0.5 μm IBA. Shoots, 30 to 50 mm long, were rooted in vivo in a fogged glasshouse under 70% shade using a commerical rooting powder [IBA, 0.1% (w/w)] applied to the base of the shoots. Most (67%) of the shoots treated in this way rooted after 5 weeks. Established, rooted plants have been grown on under glasshouse conditions. Chemical names used: N6-[2-isopentenyl] adenine riboside (zeatin riboside); gibberellic acid (GA3); 6-furfurylaminopurine (kinetin); N-(phenylmethyl)-1H-purine-6-amine (BA); 1-H-indole-3-butyric acid (IBA).

scholarly journals Combined In Vitro and In Vivo Propagation for Rapid Multiplication of Grapevine cv. Arka Neelamani

HortScience ◽  
2001 ◽  
Vol 36 (6) ◽  
pp. 1107-1110 ◽  
Author(s):  
Pious Thomas ◽  
John W. Schiefelbein
Keyword(s):  
Shoot Growth ◽  
Multiplication Rate ◽  
Normal Pattern ◽  
Single Node ◽  
Rapid Multiplication ◽  
Root And Shoot Growth ◽  
Subsequent Propagation ◽  
Sprout Growth

A novel combination of in vitro and in vivo approaches was employed to generate sufficient stock of an introduced grape (Vitis vinifera L.) cv. Arka Neelamani which significantly accelerated the multiplication rate. The in vitro part included induction of root and shoot growth in shoot tip and nodal microcuttings in MS medium containing 1 μm IAA, sequential pruning of shoots at 1, 1.5, and 2 months, leaving the basal one to two nodes, resulting in fresh sprouts on the stump, and use of remaining stumps for in vivo establishment. The in vivo part included acclimatization of in vitro rooted plantlets and stumps, use of single node cuttings from 1.5- to 2-month-old in vivo shoots for the subsequent propagation, and utilizing the fresh sprout growth from these cuttings and in vivo stumps for further propagation. Employing both in vitro and in vivo approaches, we achieved a multiplication rate unparalleled to the general micropropagation or conventional propagation and significant stock was obtained within 6 months of introducing the material. The in vivo plants exhibited adult characters like distichous phyllotaxy, three lobed leaves and normal pattern of tendril development within 2 months from planting.

scholarly journals Influence of Light Conditions and Medium Composition on Morphophysiological Characteristics of Stevia rebaudiana Bertoni In Vitro and In Vivo

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 195
Author(s):  
Alla A. Shulgina ◽  
Elena A. Kalashnikova ◽  
Ivan G. Tarakanov ◽  
Rima N. Kirakosyan ◽  
Mikhail Yu. Cherednichenko ◽  
...  
Keyword(s):  
Culture Media ◽  
Stevia Rebaudiana ◽  
Adventitious Shoot ◽  
Optimal Growth ◽  
Medium Composition ◽  
Light Spectra ◽  
Stevia Rebaudiana Bertoni ◽  
Multiplication Coefficient

We investigated the influence of different conditions (light composition and plant growth regulators (PGRs) in culture media) on the morphophysiological parameters of Stevia rebaudiana Bertoni in vitro and in vivo. Both PGRs and the light spectra applied were found to significantly affect plant morphogenesis. During the micropropagation stage of S. rebaudiana, optimal growth, with a multiplication coefficient of 15, was obtained in an MS culture medium containing 2,4-epibrassinolide (Epin) and indole-3-acetic acid (IAA) at concentrations of 0.1 and 0.5 mg L−1, respectively. During the rooting stage, we found that the addition of 0.5 mg L−1 hydroxycinnamic acid (Zircon) to the MS medium led to an optimal root formation frequency of 85% and resulted in the formation of strong plants with well-developed leaf blades. Cultivation on media containing 0.1 mg L−1 Epin and 0.5 mg L−1 IAA and receiving coherent light irradiation on a weekly basis resulted in a 100% increase in the multiplication coefficient, better adventitious shoot growth, and a 33% increase in the number of leaves. S. rebaudiana microshoots, cultured on MS media containing 1.0 mg L−1 6-benzylaminopurine (BAP) and 0.5 mg L−1 IAA with red monochrome light treatments, increased the multiplication coefficient by 30% compared with controls (white light, media without PGRs).

scholarly journals Adventitious Shoot Regeneration from In Vitro Leaf Explants of the Peach Rootstock Hansen 536

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 755
Author(s):  
Angela Ricci ◽  
Luca Capriotti ◽  
Bruno Mezzetti ◽  
Oriano Navacchi ◽  
Silvia Sabbadini
Keyword(s):  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Cultures ◽  
Efficient System ◽  
Regeneration Rate ◽  
Factors Affecting

In the present study, an efficient system for the in vitro regeneration of adventitious shoots from the peach rootstock Hansen 536 leaves has been established. Twenty regeneration media containing McCown Woody Plant Medium (WPM) as a basal salt supplemented with different concentrations and combinations of plant growth regulators (PGRs) were tested. Expanded leaves along with their petiole from 3-week-old elongated in vitro shoot cultures were used as starting explants. The highest regeneration rate (up to 53%) was obtained on WPM basal medium enriched with 15.5 μM N6-benzylaminopurine (BAP). The influences on leaf regeneration of the ethylene inhibitor silver thiosulphate (STS) and of different combinations of antibiotics added to the optimized regeneration medium were also investigated. The use of 10 μM STS or carbenicillin (238 μM) combined with cefotaxime (210 μM) significantly increased the average number of regenerating shoots per leaf compared to the control. In vitro shoots were finally elongated, rooted and successfully acclimatized in the greenhouse. The results achieved in this study advances the knowledge on factors affecting leaf organogenesis in Prunus spp., and the regeneration protocol described looks promising for the optimization of new genetic transformation procedures in Hansen 536 and other peach rootstocks and cultivars.

In vitro studies of adventitious shoot formation in Pinus contorta

1981 ◽  
Vol 59 (5) ◽  
pp. 870-874 ◽  
Author(s):  
Sara Von Arnold ◽  
Tage Eriksson
Keyword(s):  
Pinus Contorta ◽  
Stem Elongation ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Adventitious Buds ◽  
Adventitious Shoot Formation ◽  
Bud Induction ◽  
Further Development

Isolated embryos of Pinus contorta Dougl. ex Loud, were induced to form adventitious buds on a cytokinin-supplemented medium. Further development of the buds required transfer to a cytokininless medium. Both bud induction and development were stimulated by a dilution of the basal culture medium and best growth was obtained if the buds were isolated from the original tissue when stem elongation had started. The growth of these isolated adventitious shoots was further stimulated by adding activated charcoal to the diluted medium. A small percentage of the shoots have been rooted. The capacity for bud formation varied among seeds collected from different regions of British Columbia. This method for induction of adventitious buds on embryos was also applicable to explants of young seedlings.

scholarly journals 883 PB 279 REGENERATING ADVENTITIOUS PLANTS FROM IN VITRO CULTURE OF LIATRIS SPICATA (L.) WILLD. COTYLEDONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560d-560
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Adventitious Shoot Formation ◽  
Murashige Skoog ◽  
Micropropagated Plants

Cotyledons from developing embryos 6 to 8 weeks old of Liatris spicata (blazing star) were cultured on Murashige-Skoog (MS) medium containing 0, 0.4, 4.4, and 44.4 μ M benzyladenine (BA) or 0, 0.2, 2.2, and 22.2 μ M thidiazuron (TDZ) to induce adventitious shoot formation. The highest percent of cotyledons forming shoots with highest shoot counts was on medium containing 2.2 μ M TDZ. Vitreous shoots formed on medium with 22.2 μ M TDZ. Callus derived from cotyledons and cultured on medium containing 4.44 μ M BA or 2.2 μ M TDZ formed adventitious shoots with highest shoot counts on 4.44 μ M BA. Adventitious shoots derived from cotyledons and callus were rooted on MS medium with 5.0 μ Mindole-3-butyric acid, acclimatized and grown ex vitro. All micropropagated plants appeared similar to each other.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

scholarly journals Adventitious Shoot Formation and Plant Regeneration from Bell Pepper (Capsicum annuum L.) Cultivars and Dihaploid Lines

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 471B-471
Author(s):  
Agustin Huerta ◽  
Ramon Dolcet-Sanjuan
Keyword(s):  
Capsicum Annuum ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Bell Pepper ◽  
Induction Phase ◽  
Adventitious Shoot Formation ◽  
Shoot Primordia

Adventitious shoots and viable plants were regenerated from bell pepper (Capsicum annuum L.) cultivars and dihaploid lines (DHLs) obtained from F1 hybrids via androgenesis (Dolcet-Sanjuan et al., in press). Hypocotil and cotyledon sections from in vitro-germinated seeds were used as explants. A modified MS medium (Murashige and Skoog, 1962) supplemented with IAA (0 to 3.2 μM) and BAP (0 to 100 μM) was used in a 3-week-long shoot primordia induction phase. Shoot elongation was best performed in the same basal medium, but supplemented with silver thiosulfate and GA3. Shoots were regenerated from eight selected DHLs (`C213', `C215', `C218', `C2123', `C2125', `C3111', `C3113', and `P493') and two cultivars (`Padrón' and `Yolo Wonder'). The percentage of cotyledon sections with shoot primordia after the induction phase was not genotype-dependent and always higher than with hypocotil sections (93.4% and 17.9%, respectively). The number of shoot primordia per responsive cotyledon section was also higher than with hypocotil sections (3.3 and 1.7, respectively). The genotype had a significant effect on the number of shoots regenerated per responsive cotyledon (1.1 to 5.5) or hypocotil (0.5 to 3.5) section. All adventitiously regenerated plants were fertile. This adventitious shoot regeneration protocol is being used to obtain transgenic plants from sweet bell pepper genotypes.

scholarly journals Interaction between Explant Size and Cultivar Affects Shoot Organogenic Competence of Watermelon Cotyledons

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 749-750 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Shoot Regeneration ◽  
Significant Interaction ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Proximal Region ◽  
Specific Region ◽  
Adventitious Shoot Regeneration ◽  
Cotyledon Explants

Organic competence of different explant sizes and locations on watermelon seedlings was determined by calculating the percentage of cotyledon explants that produced adventitious shoots. About 52% (214/412) of explants prepared from the proximal region of cotyledons formed shoots, whereas only ≈6% (24/411) of distal explants did so. Shoot formation was limited to the proximal end of basal explants but was not restricted to any specific region on distal ones. The percentage of explants that produced harvestable shoots was greater from basal halves than basal quarters in `Sweet Gem', `Crimson Sweet', and `Minilee', but explant size did not affect adventitious shoot regeneration of `Yellow Doll', resulting in significant interaction between cultivar and explant size. This study indicates that cultivars that respond poorly to in vitro procedures may have fewer cells competent for shoot regeneration, requiring special care during explant preparation.

scholarly journals Histological changes during the adventitious shoot formation in seedling explants of pepper (Capsicum annuum L.) cultured in vitro

2014 ◽  
Vol 71 (4) ◽  
pp. 269-274
Author(s):  
Andrzej Gatz
Keyword(s):  
Capsicum Annuum ◽  
Adventitious Shoots ◽  
Cotyledonary Node ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Explant Culture ◽  
Mesophyll Cells ◽  
Adventitious Shoot Formation ◽  
Seedling Explants

Adventitious shoots differentiated directly from explant tissue without intermediate callus on all types of examined explants (shoot tip, cotyledonary node, cotyledon and hypocotyl) of <em>Capsicum annuum</em> L. cv. Bryza. First cell divisions took place as early as after 3 days of explant culture within epidermal and subepidermal layers of explants, and in the case of cotyledon also within mesophyll cells located near epidermis. Mitotic activity in these layers led to the formation of meristemoids (meristematic centres). In all types of studied explants, meristematic centres appeared approximately at the same time (after about 7 days of culture). In the second week bud primordia began to differentiate from meristematic centres. Subsequently some of shoot primordia developed into leaves and leaf-like structures (mainly on cotyledon explants), and also into adventitious buds with well developed apical meristem and leaf primordia.

Sign in / Sign up

Export Citation Format

Share Document