scholarly journals In Vitro Germination of Immature Prunus lusitanica Seed

HortScience ◽  
2017 ◽  
Vol 52 (8) ◽  
pp. 1122-1124 ◽  
Author(s):  
Justin A. Schulze ◽  
Jason D. Lattier ◽  
Ryan N. Contreras
Keyword(s):  
Tissue Culture ◽  
Gibberellic Acid ◽  
Cold Stratification ◽  
In Vitro Germination ◽  
Seed Conditioning ◽  
Shoot Emergence ◽  
Culture Protocol

A tissue culture protocol was developed to germinate immature Prunus lusitanica seeds in vitro. The study was conducted by first identifying the best media for germination, followed by investigating effects of seed conditioning. In Expt. I, seeds were collected 12 weeks after pollination (WAP) ± 1 week and placed on media after removing the pericarp. Eight different MS media (Murashige and Skoog, 1962) were tested (M1–M8) containing two concentrations each of 6-benzylaminopurine (BA), gibberellic acid (GA3), and sucrose. The longest shoots resulted from M4 (1.45 µm GA3, 6 µm BA, and 30 g·L−1 sucrose), followed by M1 (0 µm GA3, 3 µm BA, and 30 g·L−1 sucrose). Radicle and shoot emergence was greater than or equal to 90% for M1, M3, and M4 after a stratification treatment. In Expt. II, M1 was used to test root and shoot emergence at 6, 9, and 12 WAP, with and without cold stratification. Little success was seen 6 and 9 WAP, with only callus development in 6 WAP, nonstratified seed. Cold stratification increased shoot emergence in the 12 WAP group from 4% to 28%, appearing to be critical for shoot emergence. If the cotyledons are retained on the seed, future efforts to expedite breeding of P. lusitanica using in vitro germination should not be collected before 12 WAP and will benefit from cold stratification before germinating on M1 or M4. Chemical names: 6-benzylaminopurine (BA), gibberellic acid (GA3).

scholarly journals Effect of stratification and gibberellic acid on epicotyl dormancy release in seeds of Yunnanopilia longistaminea (Opiliaceae)

2016 ◽  
Author(s):  
Guan-song Yang ◽  
Liu Yang ◽  
Yue-hua Wang ◽  
Shi-kang Shen
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Economic Value ◽  
Cold Stratification ◽  
Dormancy Release ◽  
Spring Temperature ◽  
Edible Plant ◽  
Seed Moisture Content ◽  
Radicle Emergence ◽  
Shoot Emergence

Yunnanopilia longistaminea is an endangered monotypic species belonging to Opiliaceae. This edible plant is an important germplasm source with a high economic value in China. The seed dormancy and germination of Opiliaceae species have been rarely investigated. This study examined the effects of scarification, soaking in gibberellic acid, and dehydration on the seed germination of Y. longistaminea. Results indicated that the seed germination of this species involves two stages: radicle emergence and epicotyls (shoot) emergence. During radicle emergence, the optimum temperatures were 28 °C and 28 °C/20 °C. Seed moisture content and viability decreased as dehydration occurred. Thus, the seeds may be recalcitrant. The optimum GA3 solution for the seeds undergoing shoot emergence was 100 mg·L−1. The percentages of shoot emergence in 7 and 14 days of stratification at 5 °C were slightly higher than those in other groups. This study is the first to describe epicotyl dormancy in Y. longistaminea seeds. From the seed grow to the seedling of Y. longistaminea subjected to a autumn→winter→spring temperature process in nature conditions. Warm and cold stratification can alleviate radicle and epicotyl dormancy, respectively. The duration of cold stratification also significantly affects the epicotyl dormancy release of Y. longistaminea. The researches on the seeds breaking methods: warm(28°C/20°C)→cold(5°C)→GA3(100mg·L−1)→warm(28°C/20°C).

scholarly journals Micropropagation, Micromorphological Studies, andIn VitroFlowering inRungia pectinataL.

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Developmental Changes ◽  
Bud Break ◽  
Ms Medium ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Culture Protocol ◽  
Important Medicinal Plant

A tissue culture protocol was developed for an important medicinal plantRungia pectinataL. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2solution. Murashige and Skoog (MS) medium was used to establish the cultures ofR. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L−16-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded. The shoots were further multiplied using MS medium augmented with 0.5 mg L−1each of BAP and kinetin (Kin) + 0.1 mg L−1indole-3 acetic acid (IAA). Maximum 13.2 shoots per explant with 5.2 cm length were observed. All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1indole-3 butyric acid (IBA).In vitroflowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod. The plantlets were hardened in the greenhouse for two months and finally transferred to the field. The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots underin vitroconditions.

scholarly journals Disinfection procedures for in vitro propagation of Anthurium

2015 ◽  
Vol 27 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Jaime A. Teixeira da Silva ◽  
Budi Winarto ◽  
Judit Dobránszki ◽  
Songjun Zeng
Keyword(s):  
Tissue Culture ◽  
Plant Material ◽  
In Vitro Propagation ◽  
Ex Vitro ◽  
Microbial Contaminants ◽  
Culture Protocol

Abstract Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial contaminants from the surface and interior of plant material, thus giving the explant a fighting chance at survival in vitro. Initial cultures of Anthurium species and cultivars, which are usually established from ex vitro material grown in a greenhouse, pots or in the field, easily contaminate the in vitro milieu. This review highlights the differences in disinfection protocols that exist for different species or cultivars of Anthurium. The protocol needs to be adjusted based on the material used: spadices, spathes, seeds, leaves, or roots. Regrettably, most of the currently published protocols, derived from a literature that spans over 100 published papers, have numerous weaknesses and flaws in the information provided pertaining to disinfection and infection levels. Advice for future Anthurium researchers should thus be followed cautiously.

Morphophysiological dormancy in seeds ofConvallaria keiskeiand a proposal to recognize two types of double dormancy in seed dormancy classification

2015 ◽  
Vol 25 (2) ◽  
pp. 210-220 ◽  
Author(s):  
Tetsuya Kondo ◽  
Mizuki Narita ◽  
Shyam S. Phartyal ◽  
Siti N. Hidayati ◽  
Jeffrey L. Walck ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Seed Dispersal ◽  
Cold Stratification ◽  
Negative Effects ◽  
Standard Definition ◽  
Morphophysiological Dormancy ◽  
Cotyledonary Petiole ◽  
Effects Of Temperature ◽  
Definition Of ◽  
Shoot Emergence

AbstractConvallariamajalishas double dormancy and hypogeal germination, but no information is available on embryo growth or on the effects of light and gibberellic acid (GA3) on germination in this genus. Therefore, we investigated embryo growth and other germination features in seeds ofC. keiskeiand compared the data with those ofTrillium camschatcensein another study. Until now, in seeds with double dormancy, embryo growth and germination (epigeal) have been studied in detail only for seeds ofT. camschatcense. Phenology of embryo growth and emergence of cotyledonary petiole/root (hereafter root) and shoot in seeds ofC. keiskeiwere monitored outdoors. Effects of temperature, light and GA3on embryo growth and root and shoot emergence were tested under laboratory conditions. Roots emerged the first spring following seed dispersal in autumn. The embryo grew soon after root emergence, and germination was hypogeal. Seeds with an emerged root formed buds from which a shoot (leaf) emerged above ground during the second spring. Alternating temperatures and light had negative effects on root emergence, and GA3did not substitute for cold stratification in root emergence. Seeds ofC. keiskeihave double dormancy, but it differs from that inT. camschatcense. Based on differences in embryo growth before (T. camschatcense) versus after (C. keiskei) root emergence, and on epigeal (T. camschatcense) versus hypogeal (C. keiskei) germination, we suggest that two types of deep simple double morphophysiological dormancy (MPD) be recognized. Since embryo growth inC. keiskeidoes not fit the standard definition of MPD, we propose to expand this definition.

scholarly journals In vitro germination and acclimatization of cambui tree type seedlings

2014 ◽  
Vol 44 (8) ◽  
pp. 1355-1359
Author(s):  
Ana da Silva Lédo ◽  
Luciana Borin Barin ◽  
Ana Veruska Cruz da Silva ◽  
Francielen Paola de Sá ◽  
Caroline de Araújo Machado
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Culture Media ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Coconut Husk ◽  
Yellow Type ◽  
Plastic Containers ◽  
Tree Type

There are few reports in literature on the in vitro behavior of cambui tree (Myrciaria tenella O. Berg) and acclimatization conditions. The aim of this study was to evaluate the effect of culture media on in vitro germination and the effect of different substrates on the acclimatization of two Myrciaria tenella types. The study was carried out at the Embrapa Tabuleiros Costeiros Laboratory of Plant Tissue Culture, Aracaju, SE. Seeds were extracted from fruits of two Myrciaria tenella types: Orange and Purple Types. The seeds were inoculated in the following culture media: T1 - MS medium + 30g L -1 sucrose, T2 - 1/2 MS medium + 15g L -1 sucrose and T3 - control without MS salts. To study the effect of substrates on acclimatization, seedlings were transferred to plastic containers with capacity of 300cm 3 containing the following sterilized substrates: S1 - soil and powdered coconut husk - SPC (1:1 by volume); S2 - soil, washed sand and powdered coconut husk - SAPC (1:1:1 by volume) and S3 - Biomix (r) commercial substrate - SC. The medium without MS salts promoted 100% in vitro germination and 1/2 MS medium greater development of seedlings. All substrates studied are suitable for acclimatization of seedlings germinated in vitro. Myrciaria tenella of yellow type showed greater vigor during acclimatization.

scholarly journals Micropropagation of Satureja obovata Lag.

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1278-1280 ◽  
Author(s):  
M.L. Arrebola ◽  
O. Socorro ◽  
A. Barceló-Muñoz ◽  
E. Simón-Pérez ◽  
Fernando Pliego-Alfaro
Keyword(s):  
Gibberellic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
In Vitro Germination ◽  
Free Medium ◽  
Phase Chemical

A micropropagation procedure for juvenile and adult savory (Satureja obovata Lag.) explants is described. Pretreatment of the nutlets with gibberellic acid (0.57 mm) did not improve in vitro germination. Optimum shoot proliferation of juvenile and adult material was obtained on medium containing 2.22 μm N6-benzyladenine. Rooting and acclimatization of juvenile shoots were accomplished in vivo, while adult shoots were rooted in vitro after 3 days of exposure to 4.92 μm indole-3-butyric acid followed by subsequent transfer to auxin-free medium. More than 95% survival of adult rooted plants was obtained during the acclimatization phase. Chemical names used: gibberellic acid (GA3); N6-benzyladenine (BA); indole-3-butyric acid (IBA); isopentenyladenine (2iP).

scholarly journals Effect of stratification and gibberellic acid on epicotyl dormancy release in seeds of Yunnanopilia longistaminea (Opiliaceae)

2016 ◽  
Author(s):  
Guan-song Yang ◽  
Liu Yang ◽  
Yue-hua Wang ◽  
Shi-kang Shen
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Economic Value ◽  
Cold Stratification ◽  
Dormancy Release ◽  
Spring Temperature ◽  
Edible Plant ◽  
Seed Moisture Content ◽  
Radicle Emergence ◽  
Shoot Emergence

Yunnanopilia longistaminea is an endangered monotypic species belonging to Opiliaceae. This edible plant is an important germplasm source with a high economic value in China. The seed dormancy and germination of Opiliaceae species have been rarely investigated. This study examined the effects of scarification, soaking in gibberellic acid, and dehydration on the seed germination of Y. longistaminea. Results indicated that the seed germination of this species involves two stages: radicle emergence and epicotyls (shoot) emergence. During radicle emergence, the optimum temperatures were 28 °C and 28 °C/20 °C. Seed moisture content and viability decreased as dehydration occurred. Thus, the seeds may be recalcitrant. The optimum GA3 solution for the seeds undergoing shoot emergence was 100 mg·L−1. The percentages of shoot emergence in 7 and 14 days of stratification at 5 °C were slightly higher than those in other groups. This study is the first to describe epicotyl dormancy in Y. longistaminea seeds. From the seed grow to the seedling of Y. longistaminea subjected to a autumn→winter→spring temperature process in nature conditions. Warm and cold stratification can alleviate radicle and epicotyl dormancy, respectively. The duration of cold stratification also significantly affects the epicotyl dormancy release of Y. longistaminea. The researches on the seeds breaking methods: warm(28°C/20°C)→cold(5°C)→GA3(100mg·L−1)→warm(28°C/20°C).

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