scholarly journals Storage Temperature, Controlled Atmosphere, and 1-Methylcyclopropene Effects on α-Farnesene, Conjugated Trienols, and Peroxidation in Relation with Superficial Scald, Pithy Brown Core, and Fruit Quality of ‘d’Anjou’ Pears during Long-term Storage

2016 ◽  
Vol 141 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Yan Wang
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Controlled Atmosphere ◽  
Metabolic Balance ◽  
Superficial Scald ◽  
Long Term Storage ◽  
Temperature Controlled ◽  
Term Storage

Alternatives to ethoxyquin (Etq) are needed for controlling superficial scald of ‘Anjou’ european pears (Pyrus communis) during long-term storage. The current commercial standard storage conditions [Etq + −1 °C + controlled atmosphere (CA) with 1.5 kPa O2] reduced scald occurrence compared with control fruit (−1 °C + CA) during 6–8 months storage. At 1 °C in air, 1-methylcyclopropene (1-MCP) fumigation at 0.15 µL·L−1 at harvest was more efficient on reducing scald than Etq but did not prevent scald during 6–8 months storage. The 1-MCP-treated fruit at 1 °C in air developed their ripening capacity at 20 °C following 6–8 months storage but had deceased shipping ability (softening and yellowing of fruit). Although Etq inhibition of scald was associated with the inhibition of α-farnesene oxidation to conjugated trienols (CTols); 1-MCP reduced α-farnesene synthesis and thereby the availability of substrate to oxidize to CTols. CA storage at 1.5 kPa O2 totally prevented scald and retarded the loss of shipping ability without affecting the ripening capacity of 1-MCP-treated fruit at 1 °C through further decreases in the syntheses of ethylene, α-farnesene and CTols during 6–8 months storage. In addition, 1-MCP prevented a CA-induced disorder, pithy brown core (PBC), in ‘Anjou’ pears possibly through enhancing an oxidative/reductive metabolic balance during extended storage. In conclusion, the combinations of 1 °C + 1-MCP + CA is a potential commercial alternative to Etq for scald control while allowing the 1-MCP-treated ‘Anjou’ pears to recover ripening capacity during the shelf life period after 6–8 months storage.

scholarly journals Application of Dynamic Controlled Atmosphere Technologies to Reduce Incidence of Physiological Disorders and Maintain Quality of ‘Granny Smith’ Apples

Agriculture ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 491
Author(s):  
Tatenda Gift Kawhena ◽  
Olaniyi Amos Fawole ◽  
Umezuruike Linus Opara
Keyword(s):  
Chlorophyll Fluorescence ◽  
Soluble Solids ◽  
Controlled Atmosphere ◽  
Low Oxygen ◽  
Oxygen Stress ◽  
Superficial Scald ◽  
Long Term Storage ◽  
Term Storage

The efficacy of dynamic controlled atmosphere technologies; repeated low oxygen stress (RLOS) and dynamic controlled atmosphere-chlorophyll fluorescence (DCA-CF) to control superficial scald development on ‘Granny Smith’ apples during long-term storage was studied. Fruit were stored for 2, 4, 6, 8, and 10 months at 0 °C in DCA-CF (0.6% O2 and 0.8% CO2), regular atmosphere (RA)(≈21% O2 and 90–95% RH), and RLOS treatments: (1) 0.5% O2 for 10 d followed by ultra-low oxygen (ULO) (0.9% O2 and 0.8% CO2) for 21 d and 0.5% O2 for 7 d or (2) 0.5% O2 for 10 d followed by controlled atmosphere (CA) (1.5% O2 and 1% CO2) for 21 d and 0.5% O2 for 7 d. Development of superficial scald was inhibited for up to 10 months and 7 d shelf life (20 °C) under RLOS + ULO and DCA-CF treatments. Apples stored in RLOS + ULO, RLOS + CA, and DCA-CF had significantly (p < 0.05) higher flesh firmness and total soluble solids. The RLOS phases applied with CA or ULO and DCA-CF storage reduced the development of superficial scald by possibly suppressing the oxidation of volatiles implicated in superficial scald development.

Changes in the content of organic acids and inorganic anions in sugar beet during long-term storage

2016 ◽  
pp. 760-764 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Paulina Miko ◽  
Magdalena Molska ◽  
Ilona Baszczyk ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Storage Conditions ◽  
Denitrifying Bacteria ◽  
Raw Material ◽  
Storage Duration ◽  
Long Term Storage ◽  
Term Storage ◽  
Acetic Acids

Due to the prolongation of the period of the sugar campaign, it is necessary to optimize the storage conditions, so that changes in the quality of the raw material could be minimized. The aim of this study was to determine the effect of storage duration and temperature on changes in the composition of sugar beet. The study presents the changes in the content of glucose, fructose, raffinose, lactic and acetic acids, nitrates and nitrites as well as in the content of the total number of mesophilic bacteria, denitrifying bacteria and spores of denitrifying bacteria during storage under various conditions.

Combined effects of storage temperature variation and dynamic controlled atmosphere after long-term storage of ‘Maxi Gala’ apples

2022 ◽  
Vol 31 ◽  
pp. 100770
Author(s):  
Lucas Mallmann Wendt ◽  
Vagner Ludwig ◽  
Fabiane Portella Rossato ◽  
Magno Roberto Pasquetti Berghetti ◽  
Erani Eliseu Schultz ◽  
...  
Keyword(s):  
Temperature Variation ◽  
Storage Temperature ◽  
Combined Effects ◽  
Controlled Atmosphere ◽  
Long Term Storage ◽  
Term Storage

Efficacy of the Antigenicity-Retaining Ability of Fixative Solutions for Liquid-Based Cytology: Immunocytochemistry of Long-Term Storage

2021 ◽  
pp. 1-12
Author(s):  
Hiaki Sato ◽  
Yoshiaki Norimatsu ◽  
Satoshi Irino ◽  
Takeshi Nishikawa
Keyword(s):  
Cell Membrane ◽  
Room Temperature ◽  
Storage Temperature ◽  
Storage Period ◽  
Storage Conditions ◽  
Immunocytochemical Staining ◽  
Long Term Storage ◽  
Liquid Based Cytology ◽  
Term Storage

<b><i>Introduction/Objective:</i></b> Liquid-based cytology (LBC) is advantageous as multiple stained specimens can be prepared and used for additional assays such as immunocytochemical and molecular-pathological investigations. Two types of preservative-fixative solutions (fixatives) are used for nongynecologic specimens used in the BD SurePath-LBC (SP-LBC) method, and their components vary. However, few studies have evaluated the differences in antigen-retaining ability between these fixatives. Therefore, we investigated and compared the antigen-retaining ability of the fixatives in immunocytochemical staining (ICC) under long-term storage conditions. <b><i>Materials and Methods:</i></b> Sediments of cultured RAJI cells (derived from Burkitt’s lymphoma) were added to each fixative (red and blue) and stored at room temperature for a specified period (1 h; 1 week; and 1, 3, and 6 months). The specimens were then prepared using the SP-LBC method and subjected to ICC. Positivity rate was calculated using the specimens fixed at room temperature for 1 h as a control. Antibodies against Ki67 expressed in the nucleus and against CD20 and leukocyte common antigen (LCA) expressed on the cell membrane were used. <b><i>Results:</i></b> For CD20 and LCA, the positivity rate increased with time in the red fixative compared with that in the control. In the blue fixative, the positivity rate was highest at 1 h and was maintained at a high level throughout the storage period. In contrast, the Ki67 positivity rate was highest at 1 h in both red and blue fixatives and markedly decreased with time. Therefore, although refrigerated (8°C) storage was used, no improvement was noted. <b><i>Conclusions:</i></b> Long-term storage is possible for cell membrane antigens at room temperature; however, it is unsuitable for intranuclear antigens. Therefore, we conclude that suitable fixative type and storage temperature differ based on antigen location. Further investigation is warranted.

scholarly journals Kinetic Analysis of Stabilizing C-Phycocyanin in the Spirulina platensis Extracts from Ultrasonic Process Associated with Effects of Light and Temperature

2018 ◽  
Vol 8 (9) ◽  
pp. 1662 ◽  
Author(s):  
Woon Yong Choi ◽  
Hyeon Yong Lee
Keyword(s):  
Storage Temperature ◽  
Extraction Process ◽  
Storage Conditions ◽  
Second Order ◽  
Light Illumination ◽  
First Order ◽  
Long Term Storage ◽  
Ultrasonic Process ◽  
Term Storage

A kinetic model was developed to reflect the stability of C-Phycocyanin (C-PC) from ultrasonic extraction process under different storage conditions. The decrease of C-PC contents was most accelerated at a high temperature of 40 °C along with light illumination, resulting in ca. 60% drop of an initial concentration for two months of storage. However, ca. 93% of the initial contents remained at 4 °C without light, which would be a most favorable condition for long-term storage. It was first shown that the decrease of the residual concentrations followed second-order kinetics under light illumination. However, without light, the decrease of the C-PC contents showed first order reaction kinetics, which implies initial C-PC concentrations are important. On the contrary, initial storage temperature seemed to be more influential under light illumination. It was also first revealed that the rate of degrading the C-PC was faster with light than without light even at the same temperature, having 0.0108 (1/h) and 0.0138 (1/h) of rate constants of first order (no light) and second order kinetics (with light) at 40 °C, respectively. Moreover, the cleavage of C-PC was also found to follow the Arrhenius relationship. Therefore, this work could provide desirable storage conditions of C-PC from non-thermal ultrasonic process for long-term storage.

The Effect of Storage Temperature and Physical State on the Performance of Antisera in Radioimmunoassay after Long-Term Storage

1988 ◽  
Vol 25 (1) ◽  
pp. 89-95
Author(s):  
B A Middleton ◽  
L M Morgan ◽  
G W Aherne ◽  
V Marks
Keyword(s):  
Room Temperature ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Liquid Form ◽  
Long Term Storage ◽  
Freeze Dried ◽  
Control Serum ◽  
Term Storage ◽  
Temperature Storage

The performance in radioimmunoassay of four antisera after storage at temperatures ranging from −40°C to room temperature, in three physical states (frozen, liquid or freeze dried) was investigated over a 3-year period. No deterioration in antiserum performance in terms of precision and accuracy of quality control serum measurement or recovery of ligand was apparent under any of the storage conditions studied. Some lowering of titre became apparent in two of the antisera over the study period. Deterioration was most marked when antiserum was stored lyophilised at room temperature. Storage of antiserum frozen confers no advantage over storage at 4°C provided precautions are taken to minimise bacterial contamination when storing antiserum in liquid form.

The effect of storage temperature and duration on northern red oak acorn viability and vigour

2013 ◽  
Vol 89 (06) ◽  
pp. 769-776 ◽  
Author(s):  
Thomas L. Noland ◽  
Andrée E. Morneault ◽  
Daniel C. Dey ◽  
Dave Deugo
Keyword(s):  
Storage Temperature ◽  
Storage Conditions ◽  
Red Oak ◽  
Northern Red Oak ◽  
Root Regrowth ◽  
Long Term Storage ◽  
High Germination ◽  
Percent Germination ◽  
Term Storage

Three separate collections of Ontario sources of northern red oak (Quercus rubra L.) acorns were made to determine the effects of long-term cold storage at +2°C, −1°C, and −2°C on their viability and vigour. We measured acorn moisture content, percent germination during storage, speed of germination and total germination values, root regrowth of seeds that germinated in storage, incidence of fungal contamination, and condition of ungerminated acorns. Viability and vigour peaked six to 12 months after acorns were placed in storage, but decreased with continued storage. After 18 months in storage, ≥60% of the acorns germinated in four of the five seedlots tested and, after 30 months in storage, ≥53% of the acorns germinated in three of the five seedlots tested. Acorn viability was only minimally affected by storage temperature; however, since temperatures above −2°C allowed acorns to germinate during storage, the preferred long-term storage temperature was −2°C. Our results suggest that, assuming proper storage conditions, most red oak seedlots will maintain relatively high germination levels when stored at −2°C for 18 months. Seedlots with particularly high initial germination and vigour may be successfully stored for up to 30 months.

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