Coaches in basketball often need to know how specific rotation line-ups perform in either offense or defense and choose the most efficient formation, according to their specific needs. In this research, a sample of 1131 ball possession phases of Greek Basket League was utilized, in order to estimate the offensive and defensive performance of each formation. Offensive and defensive ratings for each formation were calculated as a function of points scored or received, respectively, over possessions, where possessions were estimated using a multiple regression model. Furthermore, a Markov chain model was implemented to estimate the probabilities of the associated formation’s performance in the long run. The model could allow us to distinguish between overperforming and underperforming formations and revealed the probabilities over the evolution of the game, for each formation to be in a specific rating category. The results indicated that the most dominant formation, in terms of offense, is Point Guard-Point Guard-Small Forward-Power Forward-Center, while defensively schema Point Guard-Shooting Guard-Small Forward-Center-Center had the highest rating. Such results provide information, which could operate as a supplementary tool for the coach’s decisions, related to which rotation line-up patterns are mostly suitable during a basketball game.