scholarly journals Phytochemical Screening and Antioxidant Potential of Selected Nigerian Vegetables

2019 ◽  
Vol 8 (1) ◽  
pp. 12-16
Author(s):  
Yusuf Hassan ◽  
Magaji Ilu Barde
Keyword(s):  
Reducing Power ◽  
Antioxidant Potential ◽  
Phytochemical Screening ◽  
Antioxidant Capacities ◽  
The Individual

Reducing power activities of ten vegetables commonly consumed in Katsina State, Nigeria have been assessed with the view of establishing their antioxidant capacities. Preliminary, the phytochemicals of the individual plants were screened, and it was found that various constituents were present. In particular, flavonoids and anthraquinones were found in Ficus glumosa which also appeared to demonstrate the best reducing power activity (4.898 at 25 mg/L).

Turmeric and cinnamon dominate in antioxidant potential among four major spices

2017 ◽  
Vol 26 (1) ◽  
pp. 27 ◽  
Author(s):  
B Saranya ◽  
T Sulfikarali ◽  
S Chindhu ◽  
A M Muneeb ◽  
N K Leela ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Reducing Power ◽  
Black Pepper ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Power Method ◽  
Phenol Content ◽  
Dpph Assay ◽  
Synthetic Antioxidant

Antioxidant activity of sequential extracts of black pepper, ginger, turmeric and cinnamon was determined by DPPH assay, phosphomolybdate method and ferric reducing power method and compared with that of the synthetic antioxidant BHA. The results revealed that methanol extract of cinnamon has highest antioxidant potential followed by chloroform extract of turmeric. The antioxidant potential was also correlated with total phenol content.  

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

Design and Synthesis of Novel Anti-inflammatory/Anti-ulcer Hybrid Molecules with Antioxidant Activity

2020 ◽  
Vol 16 ◽  
Author(s):  
Bhim Bahadur Chaudhari ◽  
Alka Bali ◽  
Ajitesh Balaini
Keyword(s):  
Antioxidant Activity ◽  
Oral Absorption ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Inflammatory Activity ◽  
In Vivo Models ◽  
Standard Drug ◽  
Hybrid Molecules ◽  
Anti Inflammatory

Background: NSAIDs are the most widely prescribed medications worldwide for their anti-inflammatory, antipyretic, and analgesic effects However, their chronic use can lead to several adverse drug events including GI toxicity. The selective COX-2 inhibitors developed as gastro-sparing NSAIDs also suffer from serious adverse effects which limit their efficacy. Objective: Local generation of reactive oxygen species is implicated in NSAID-mediated gastric ulceration and their combination with H2 antagonists like famotidine reduces the risk of ulcers. The objective of this work was to design and synthesize novel methanesulphonamido isoxazole derivatives by hybridizing the structural features of NSAIDs with those of antiulcer drugs (ranitidine, famotidine, etc.) to utilize a dual combination of anti-inflammatory activity and reducing (antioxidant) potential. Method: The designing process utilized three dimensional similarity studies and utilized an isoxazole core having a potential for anti-inflammatory as well as radical scavenging antioxidant activity. The compounds were assayed for their antiinflammatory activity in established in vivo models. The in vitro antioxidant activity was assessed in potassium ferricyanide reducing power (PFRAP) assay employing ascorbic acid as the standard drug. Results: Compounds (5, 6, 9 and 10) showed anti-inflammatory activity comparable to the standard drugs and were also found to be non-ulcerogenic at the test doses. Compounds 6-10 exhibited good antioxidant effect in the concentration range of 1.0-50.0 µmol/ml. The test compounds were also found to comply with the Lipinski rule suggesting good oral absorption. Conclusion: A new series of isoxazole based compounds is being reported with good anti-inflammatory activity coupled with antioxidant potential as gastro-sparing anti-inflammatory agents.

PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF AEGLE MARMELOS FRUIT

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 59-64
Author(s):  
Pratibha Thaware ◽  
Pushpa Karale ◽  
Mahesh Karale ◽  
Pranati Chavan
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Pharmacological Action ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Aegle Marmelos ◽  
Pharmacological Screening ◽  
Hplc Data

The objective of this study was to evaluate the phytochemical and pharmacological screening of fruit extracts of Aegle marmelos using various in vitro methods. The antioxidant activity of the extracts was evaluated by using DPPH free radical scavenging and reducing power method, while well diffusion method was used for antimicrobial activity. Phytochemical screening indicated the presence of flavonoids, and phenolics were abundantly present in methanolic and aqueous extracts of A. marmelos. Methanolic extract showed significant antioxidant potential, which was however inferior to that of ascorbic acid. The methanolic extract showed maximum zone of inhibition against the S. aureus (18 mm) and it was higher than that of the standard tetracycline. The HPLC data predict that the presence of scopoletin, marmelosin and umbelliferone, which may contribute to their pharmacological action.

scholarly journals Evaluation of Phytochemical Content and Antioxidant Potential of Ocimum gratissimum and Telfairia occidentalis Leaves

2019 ◽  
pp. 1-11
Author(s):  
A. I. Airaodion ◽  
A. H. Ibrahim ◽  
U. Ogbuagu ◽  
E. O. Ogbuagu ◽  
O. O. Awosanya ◽  
...  
Keyword(s):  
Pharmacological Study ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Running Water ◽  
Phytochemical Content ◽  
Ocimum Gratissimum ◽  
Telfairia Occidentalis ◽  
Significant Difference ◽  
Ibadan Nigeria

Aim: The aim of this study is to compare the phytochemical content and antioxidant potential of Ocimum gratissimum and Telfairia occidentalis leaves. Study Design: This study was made to fit a one-way Analysis of Variance. Place and Duration of Study: This research was carried out in the Department of Premedical Science, Educational Advancement Centre, Ibadan and Pharmaceutical Laboratory of the University of Ibadan, Nigeria between January and June, 2018. Methods: Both plants were purchased from Bodija market in Ibadan, Nigeria. The leaves were removed from the stem and washed with running water to remove contaminants. It was oven dried at 37˚C and milled into powder and extracted with ethanol. The qualitative and quantitative analyses of the phytochemical content as well as antioxidant potential were investigated. Results: The result showed that O. gratissimum is significantly higher in flavonoids content but lower in alkaloids when compared with those of T. occidentalis at P<0.05. No significant difference was observed in the concentrations of saponin, tannin, total phenolics and phytic acid in O. gratissimum when compared with those of T. occidentalis respectively at P<0.05. Antioxidant investigation showed that O. gratissimum is higher in ferric-ion reducing power but lower in ascorbic acid when compared with T. occidentalis respectively at P<0.05. The percentage inhibition of 2,2-diphenyl-1-picryl-hydrazyl-hydrate radical scavenging potential was observed to decrease with decreasing concentration for both plants but that of O. gratissimum was lower when compared with that of T. occidentalis respectively. Conclusion: This pharmacological study is a useful tool for further drug development from the natural plant products.

Pulicaria laciniata (Coss and Kral): Effects of Different Extraction Solvents on Phytochemical Screening and Antioxidant Activity

2021 ◽  
pp. 425-430
Author(s):  
Kamilia Bireche ◽  
Hocine Dendougui ◽  
Asma Abid ◽  
Abdeldjabbar Messaoudi ◽  
Mohamed Hadjadj
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
The Other ◽  
Phytochemical Screening ◽  
Butanol Extract ◽  
Metal Chelating ◽  
Phytochemical Investigation ◽  
Ic50 Value ◽  
Extraction Solvents ◽  
Phytochemical Constituents

This study aims to investigate phytochemical constituents of Pulicaria laciniata extracts and determine their antioxidant activity using three methods; Phosphomolybdate, Reducing Power, and Metal Chelating. The phytochemical investigation showed various secondary metabolites such as Phenols, Glycosides, Flavonoids, Alkaloids, Tannins, and Terpenoids. The N-butanol extract exhibited the highest antioxidant activity comparing with the other extract in all methods (0.51 and 0.65 mg/ml as A0.5 values of Phosphomolybdate, reducing power) and (1.65mg/ml for IC50 value of metal-chelating). In contrast, all the extracts showed week activity against the metal-chelating method.

Zawartość związków fenolowych w ekstrakcie z propolisu oraz ocena jego aktywności przeciwutleniającej i cytoochronnej względem erytrocytów ludzkich w warunkach stresu oksydacyjnego in vitro

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Marta Babicka ◽  
Elżbieta Hołderna-Kędzia ◽  
...  
Keyword(s):  
Free Radicals ◽  
Phenolic Acids ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Natural Material ◽  
Propolis Extract

Introduction. Propolis (bee glue) is a natural product collected by honeybees from buds of various trees, shrubs and other plant species. Extracts of propolis possess numerous biological activities, including antioxidant, antibacterial, antifungal and anticancer. For this reason, propolis is currently used in many applications, such as preparations for cold syndrome, dermatological preparations or as a constituent of nutritional supplements and health food. The chemical composition of this natural material is very complex and depending on many factors, including method of extraction and selection of the solvent for the extraction process. Aim. The aim of the study was to determine concentration of selected phenolic compounds (flavonoids and phenolic acids) in extract of Polish propolis and estimate its antioxidant activity and effect on human red blood cells. Material and methods. In the propolis extract was determined concentration of 14 flavonoids and 9 phenolic acids using ultra-performance liquid chromatography equipped with a photodiode detector and a triple quadrupole mass spectrometer. The antioxidant potential of propolis extract was evaluated applying DPPH˙ free radical scavenging activity assay and Fe3+ reducing power assay. Moreover, the cytotoxicity and cytoprotective potential of propolis extract was estimated using human erythrocytes in vitro. Results. The propolis extract contained high concentration of pinocembrin, galangin, chrysin, apigenin, kaempferol, coumaric acid and cinnamic acid. It exhibited also high antioxidant potential. The antiradical activity of examined propolis extract was equal to 75% approx. activity of both standard antioxidants used in the study, namely Trolox and BHT. The reducing power of extract was equal to 65% approx. of Trolox and 80% of BHT, respectively. The propolis extract had no hemolytic activity, moreover, effectively protected human erythrocytes against free radicals-induced damage in vitro. Conclusions. The results of this study indicate that the propolis extract of national origin is a rich source of flavonoids and phenolic acids. Therefore, the propolis extract possesses a high antioxidant potential and can protect erythrocytes against free radicals-induced oxidative hemolysis.

scholarly journals Effect of the Solvent on Propolis Phenolic Profile and its Antifungal, Antioxidant, and In Vitro Cytoprotective Activity in Human Erythrocytes Under Oxidative Stress

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4266
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Patrycja Kwaśniewska-Sip ◽  
Agnieszka Waśkiewicz ◽  
Piotr Nowak ◽  
...  
Keyword(s):  
Free Radical ◽  
Antifungal Activity ◽  
Biological Effects ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Phenolic Profile ◽  
Cytoprotective Activity

Propolis is a natural bee product with various beneficial biological effects. The health-promoting properties of propolis depend on its chemical composition, particularly the presence of phenolic compounds. The aim of this study was to evaluate the relationship between extraction solvent (acetone 100%, ethanol 70% and 96%) and the antifungal, antioxidant, and cytoprotective activity of the extracts obtained from propolis. Concentrations of flavonoids and phenolic acids in the propolis extracts were determined using ultrahigh-performance liquid chromatography. The antioxidant potential of different extracts was assessed on the basis of 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free-radical-scavenging activity, Fe3+-reducing power, and ferrous ion (Fe2+)-chelating activity assays. The ability of the extracts to protect human red blood cell membranes against free-radical-induced damage and their antifungal activity was also determined. The results showed that the concentration of flavonoids in the propolis extracts was dependent on the solvent used in the extraction process and pinocembrin, chrysin, galangin, and coumaric acid were the most abundant phenols. All extracts exhibited high antioxidant potential and significantly protected human erythrocytes against oxidative damage. On the other hand, the antifungal activity of the propolis extracts depended on the solvent used in extraction and the fungal strains tested. It needs to be stressed that, to the best of our knowledge, there is no study relating the effect of solvent used for extraction of Polish propolis to its phenolic profile, and its antifungal, antioxidant, and cytoprotective activity.

In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum

2009 ◽  
Vol 64 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
Hari Datta Bhattarai ◽  
Babita Paudel ◽  
Hong Kum Lee ◽  
Hyuncheol Oh ◽  
Joung Han Yim
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Commercial Application ◽  
Moss Species ◽  
Antioxidant Capacities ◽  
Antioxidant Agents ◽  
Antarctic Moss

Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe3+) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application.

scholarly journals GC-MS Analysis and Antioxidant Potential of Essential Oil from Endemic Sideritis rubriflora Hub.-Mor.

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 24
Author(s):  
Ceylan ◽  
Zengin ◽  
Aktümsek
Keyword(s):  
Essential Oil ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Oil Composition ◽  
Antioxidant Potential ◽  
Oil Composition ◽  
Metal Chelating ◽  
Natural Agents ◽  
Mediterranean Regions ◽  
Shed Light

The genus Sideritis, belonging to the Labiateae family, is represented by more than 150 species distributed mainly in the Mediterranean regions. Member of the genus have been used as beverages, flavorings, and medication. We aimed to shed light on the antioxidant potential of the essential oil of Sideritis rubriflora Hub.-Mor. In this study, antioxidant capacity (DPPH and ABTS radical scavenging, CUPRAC and FRAP, phosphomolibdenum assay, and metal chelating activity) of essential oil of S. rubriflora was investigated with colorimetric methods. Also, essential oil composition of the plant was determined by gas chromatography/mass spectrophotometry (GC-MS). 48 components, representing 95.4% of essential oil of S. rubriflora were identified. β-pinene (10.7%) and Germacrene D (10.7%) were the main constituents of the essential oil. Generally, essential oil of S. rubriflora has shown moderate free radical, reducing power, metal chelating. The reported results supported that the possible use of essential oil of S. rubriflora is a source of natural agents for phytopharmaceutical applications.

Sign in / Sign up

Export Citation Format

Share Document