Use of Azospirillum brasilense Sp245 to Increase the Efficacy of Clonal Micropropagation of Cretaceous Catchfly (Silene cretacea Fisch. ex Spreng)

2017 ◽  
pp. 72-79 ◽  
Author(s):  
T.A. Kritskaya ◽  
◽  
N.V. Evseeva ◽  
G.L. Burygin ◽  
A.S. Kashin ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Clonal Micropropagation

Research of carbohydrate type influence on Lonicera caerulea l. Morphogenetic potential in vitro

2020 ◽  
Vol 61 ◽  
pp. 54-60
Author(s):  
N. D. Orlova ◽  
D. A. Egorova
Keyword(s):  
Multiplication Factor ◽  
Lonicera Caerulea ◽  
Genetic Characteristics ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The clonal micropropagation technique of valuable Lonicera caerulea L. varieties (Indigo Gem, Moskovskaya 23, Diana, Jugana) has been improved. The effect of various types and concentrations of carbohydrates on microshoots regeneration of the genus Lonicera L. representatives was studied. It is shown that the concentration of carbohydrates has the greatest effect on microshoots height and the type of carbohydrate nutrition has the greatest effect on multiplication factor. The type of carbohydrate nutrition (42 %) had the greatest influence on multiplication factor. Peculiarities of varieties, genetic characteristics interaction, carbohydrate nutrition type and concentration had a lesser effect (20 %, 14 % and 11 % respectively). The variety Moskovskaya 23 was characterized by the highest microshoots number (49.6 mm). The highest multiplication factor in the studied varieties, regardless of the type of carbohydrate nutrition, was observed at concentration of 40 g/l (11.57).

Снижение эффективности формирования биопленок и их устойчивости к окислительному стрессу и высушиванию у ahpC мутанта Azospirillum Brasilense

2021 ◽  
Vol 90 (1) ◽  
pp. 64-75
Author(s):  
А. В. Шелудько ◽  
Д. И. Мокеев ◽  
С. С. Евстигнеева ◽  
Ю. А. Филипьечева ◽  
А. М. Буров ◽  
...  
Keyword(s):  
Azospirillum Brasilense

scholarly journals SOYBEAN CULTIVATION IN A CROP-LIVESTOCK SYSTEM WITH Azospirillum brasilense INOCULATION

2018 ◽  
Vol 31 (1) ◽  
pp. 1-8
Author(s):  
MARCOS DA SILVA BRUM ◽  
THOMAS NEWTON MARTIN ◽  
VINÍCIUS SANTOS DA CUNHA ◽  
LUIZ FERNANDO TELEKEN GRANDO ◽  
ALEX TAGLIAPIETRA SCHONELL
Keyword(s):  
Azospirillum Brasilense ◽  
Block Design ◽  
Experimental Period ◽  
Height Control ◽  
Grain Productivity ◽  
Randomized Complete Block Design ◽  
Grazing Systems ◽  
Soybean Plants ◽  
Santa Maria ◽  
Livestock System

ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.

scholarly journals Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
José Francisco Cruz-Pérez ◽  
Roxana Lara-Oueilhe ◽  
Cynthia Marcos-Jiménez ◽  
Ricardo Cuatlayotl-Olarte ◽  
María Luisa Xiqui-Vázquez ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Type Strain ◽  
Wild Type Strain ◽  
Soil Conditions ◽  
Wild Type ◽  
Gene Encoding ◽  
Wheat Roots ◽  
Genes Encoding ◽  
In The Wild ◽  
Plant Growth Promoting

AbstractThe plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO3 or NH4Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.

GLUTAMATE SYNTHASE FROM AZOSPIRILLUM BRASILENSE: ROLE OF FLAVINS AND IRON SULFUR CENTERS

1991 ◽  
pp. 749-754
Author(s):  
Maria A. Vanoni ◽  
Giuliana Zanetti ◽  
Bruno Curti ◽  
Dale E. Edmondson
Keyword(s):  
Azospirillum Brasilense ◽  
Glutamate Synthase ◽  
Iron Sulfur

Zinc use efficiency of maize-wheat cropping after inoculation with Azospirillum brasilense

2021 ◽  
Author(s):  
Fernando Shintate Galindo ◽  
João Leonardo Miranda Bellotte ◽  
José Mateus Kondo Santini ◽  
Salatiér Buzetti ◽  
Poliana Aparecida Leonel Rosa ◽  
...  
Keyword(s):  
Azospirillum Brasilense ◽  
Use Efficiency
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