scholarly journals Inter-Specific Variation in SDS-PAGE Electrophograms of Total Leaf Proteins in Some Species of Subtribe Cassiinae

2019 ◽  
Vol 10 (1) ◽  
pp. 1-11
Author(s):  
Kolawole S. ◽  
Abdulrahaman A.
Keyword(s):  
Leaf Proteins ◽  
Total Leaf ◽  
Sds Page ◽  
Specific Variation

Proteomics Variation in Nigella Sativa L. (Rananculaceae) Germplasm

2021 ◽  
Vol 50 (2) ◽  
pp. 289-294
Author(s):  
Muhammad Sajjad Iqbal ◽  
Abdul Ghafoor
Keyword(s):  
Euclidean Distance ◽  
Nigella Sativa ◽  
Crop Improvement ◽  
Band Formation ◽  
First Report ◽  
Sds Page ◽  
Genetic Pattern ◽  
Specific Variation ◽  
Improvement Programs ◽  
Single Seeds

Study revealed a first report of proteomics variation in Nigella sativa L. based on analyzing 32 accessions through SDS-PAGE. Three prominent regions along eight subunits were identified. Intra specific variation was observed low whereas the sharpness of bands was high between first and second regions. It was noted that in second region there was no clear evidence of band formation in N. sativa. Prominent and sharp protein peptide bands were recorded in four accessions, namely PK-020561, PK-020609, PK-020620 and PK-020646. Further investigation of single seeds showed almost similar genetic pattern within the single accession. Five clusters were formed on the basis of Euclidean distance. Cluster-I & II contain 1, 1 accession each, likewise Cluster-III and C-IV contain 2, 2 accessions whereas Cluster-V was found diversified as consisted of 26 accessions. Two accessions PK-020878 and PK-020877 were recommended for polymorphism and crop improvement programs. Bangladesh J. Bot. 50(2): 289-294, 2021 (June)

scholarly journals ntra-species profiling of Cleome viscosa growing in Swat district (Pakistan)

2018 ◽  
Vol 26 (1) ◽  
pp. 52-55
Author(s):  
N. Muhammad ◽  
S. F. Wadood ◽  
W. Khan ◽  
N. Ali ◽  
M. Nisar
Keyword(s):  
Allelic Variation ◽  
Lower Surface ◽  
Protein Profiling ◽  
Yellow Colour ◽  
As Species ◽  
Sds Page ◽  
Specific Variation ◽  
Species Specific ◽  
High Level ◽  
Cleome Viscosa

Intra-specific genetic variation was studied in 28 genotypes of Cleome viscosa L. growing in Swat district, Khyber Pakhtunkhwa, Pakistan. It was found that genotypes showed the utmost allelic variation for leaf upper and lower surface with emerald green (75%), and yellow green (75%) respectively, other leaves lower and upper surfaces were (25%) green and yellow green (26%) respectively. The majority of C. viscosa genotypes were (50%) yellow flowers while others were with (29%) white yellow colour and (21%) dull yellow. Most of the seeds were with black (46%). The protein profiling was carried out on 12% gel electrophoresis; seven reproducible bands with molecular weight ranges from 180 to 10 KDa were detected in C. viscosa, the locus contribution toward genetic disagreement (LCTGD) of C. viscosa was 57%. Notably, L-3, L-4 L-5, was monomorphic in C. viscosa and was treated as species specific. L-1, L-2, L-7 were polymorphic. These bands showed 79%, 4%, 14% and 79% variation respectively. In the current investigation the intra-specific variation was observed limited and alone SDS-PAGE did not determine the high level of intra-specific variation; however, diverse germplasm were suggested to be acquired from various sources.

scholarly journals SDS-PAGE Characterisation of Crude Seed and Leaf Proteins in Corchorus Species

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Christiana Adeyinka AJALA ◽  
Joseph Akintade MORAKINYO
Keyword(s):  
Leaf Proteins ◽  
Sds Page

scholarly journals SDS-PAGE Characterisation of Crude Seed and Leaf Proteins in Corchorus Species

2015 ◽  
Vol 7 (2) ◽  
pp. 177-183
Author(s):  
Christiana Adeyinka AJALA ◽  
Joseph Akintade MORAKINYO
Keyword(s):  
Protein Separation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Electrophoretic Study ◽  
Single Linkage ◽  
Banding Patterns ◽  
Leaf Proteins ◽  
Sds Page ◽  
Single Linkage Cluster ◽  
Linkage Cluster

Crude protein separation was carried out for Corchorus incisifolious, Corchorus aestuans, Corchorus tridens and Corchorus olitorious using Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Plants were collected both from wild and cultivated sites and samples included leaves and seeds for the electrophoretic study. Distinct polymorphism in electrophoretic banding patterns of seed and leaf proteins following SDS- PAGE was observed in the four Corchorus species studied. Forty- two polypeptide bands were observed in the seed and a total of eleven polypeptide bands were observed in the leaves of the Corchorus species studied. The electrophoretic study revealed protein bands with various intensities ranging from high, to low and faint. The results showed that there was variation in both the seed and leaf proteins of the Corchorus species studied. A dendrogram constructed based on the Single Linkage Cluster Analysis (SLCA) clustering method revealed three major clusters for seeds. Cluster I consisted of C. incisifolious and C. aestuans, cluster II consisted of C. tridens, while cluster III consisted of C. olitorious. The leaf protein extracts were grouped into two clusters, cluster one containing C. incisifolious and C. aestuans, while the other contained C. tridens and C. olitorious.

Inter and Intra-Specific Variation in SDS-PAGE of Total Seed Protein in Rice (Oryza sativa L.) Germplasm

2004 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Rehana Asghar ◽  
Rabia Siddique . ◽  
Muhammad Afzal . ◽  
Shamim Akhtar .
Keyword(s):  
Oryza Sativa ◽  
Seed Protein ◽  
Oryza Sativa L ◽  
Sds Page ◽  
Specific Variation ◽  
Total Seed

3-D organization of fibrinogen receptors using computer reconstruction and whole-mount microscopy

1988 ◽  
Vol 46 ◽  
pp. 30-31
Author(s):  
E. T. O'Toole ◽  
R. R. Hantgan ◽  
J. C. Lewis
Keyword(s):  
Whole Blood ◽  
Colloidal Gold ◽  
Blood Platelets ◽  
Cell Contact ◽  
Computer Assisted ◽  
Adherent Cells ◽  
Differential Centrifugation ◽  
Computer Reconstruction ◽  
Sds Page ◽  
Whole Mount

Thrombocytes (TC), the avian equivalent of blood platelets, support hemostasis by aggregating at sites of injury. Studies in our lab suggested that fibrinogen (fib) is a requisite cofactor for TC aggregation but operates by an undefined mechanism. To study the interaction of fib with TC and to identify fib receptors on cells, fib was purified from pigeon plasma, conjugated to colloidal gold and used both to facilitate aggregation and as a receptor probe. Described is the application of computer assisted reconstruction and stereo whole mount microscopy to visualize the 3-D organization of fib receptors at sites of cell contact in TC aggregates and on adherent cells.Pigeon TC were obtained from citrated whole blood by differential centrifugation, washed with Ca++ free Hank's balanced salts containing 0.3% EDTA (pH 6.5) and resuspended in Ca++ free Hank's. Pigeon fib was isolated by precipitation with PEG-1000 and the purity assessed by SDS-PAGE. Fib was conjugated to 25nm colloidal gold by vortexing and the conjugates used as the ligand to identify fib receptors.

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