Possible Short-Term Biological Effects of Kefir Beverage: Ⅰ: Effect of Kefir Beverage on The Cell Biological, Histochemical, Histopathological and Biochemical Changes in Pancreas of High Fat-Fed STZ- Induced Diabetic Male Wistar Rat

Chronic high fat feeding is correlated with diabetes and kidney disease. However, the impact of short-term high fat diets (HFD) is not well-understood. Six weeks of HFD result in indices of metabolic syndrome (increased adiposity, hyperglycemia, hyperinsulinemia, hyperlipidemia, hyperleptinemia, and impaired endothelium-dependent vasodilation) compared to rats fed on standard chow. The hypothesis was that short-term HFD would induce early signs of renal disease. Young male Sprague-Dawley rats were fed either HFD (60% fat) or standard chow (5% fat) for six weeks. Morphology was determined by measuring changes in renal mass and microstructure. Kidney function was measured by analyzing urinary protein, creatinine, and hydrogen peroxide (H2O2) concentrations, as well as plasma cystatin C concentrations. Renal damage was measured through assessment of urinary oxDNA/RNA concentrations as well as renal lipid peroxidation, tumor necrosis factor alpha (TNFα), and interleukin 6 (IL-6). Despite HFD significantly increasing adiposity and renal mass, there was no evidence of early stage kidney disease as measured by changes in urinary and plasma biomarkers as well as histology. These findings suggest that moderate hyperglycemia and inflammation produced by short-term HFD are not sufficient to damage kidneys or that the ketogenic HFD may have protective effects within the kidneys.

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In adipose tissue, increased mitochondrial emission of reactive oxygen species is important for short-term high-fat diet-induced insulin resistance in mice

Diabetologia ◽

10.1007/s00125-015-3531-x ◽

2015 ◽

Vol 58 (5) ◽

pp. 1071-1080 ◽

Cited By ~ 50

Author(s):

Sabina Paglialunga ◽

Alison Ludzki ◽

Jared Root-McCaig ◽

Graham P. Holloway

Keyword(s):

Insulin Resistance ◽

Reactive Oxygen Species ◽

Adipose Tissue ◽

High Fat Diet ◽

Reactive Oxygen ◽

Oxygen Species ◽

High Fat ◽

Short Term

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Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skeletal muscle of healthy young men

Diabetologia ◽

10.1007/s00125-012-2717-8 ◽

2012 ◽

Vol 55 (12) ◽

pp. 3341-3349 ◽

Cited By ~ 124

Author(s):

S. C. Jacobsen ◽

C. Brøns ◽

J. Bork-Jensen ◽

R. Ribel-Madsen ◽

B. Yang ◽

...

Keyword(s):

Skeletal Muscle ◽

Dna Methylation ◽

Young Men ◽

High Fat ◽

Short Term ◽

Genome Wide

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Short-term high-fat feeding exacerbates degeneration in retinitis pigmentosa by promoting retinal oxidative stress and inflammation

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.2100566118 ◽

2021 ◽

Vol 118 (43) ◽

pp. e2100566118

Author(s):

Oksana Kutsyr ◽

Agustina Noailles ◽

Natalia Martínez-Gil ◽

Lucía Maestre-Carballa ◽

Manuel Martinez-Garcia ◽

...

Keyword(s):

Oxidative Stress ◽

Retinitis Pigmentosa ◽

Retinal Degeneration ◽

Gut Microbiome ◽

Cell Activation ◽

High Fat ◽

Short Term ◽

Oxidative Markers ◽

Degenerative Disorders ◽

Inflammatory State

A high-fat diet (HFD) can induce hyperglycemia and metabolic syndromes that, in turn, can trigger visual impairment. To evaluate the acute effects of HFD feeding on retinal degeneration, we assessed retinal function and morphology, inflammatory state, oxidative stress, and gut microbiome in dystrophic retinal degeneration 10 (rd10) mice, a model of retinitis pigmentosa, fed an HFD for 2 to 3 wk. Short-term HFD feeding impaired retinal responsiveness and visual acuity and enhanced photoreceptor degeneration, microglial cell activation, and Müller cell gliosis. HFD consumption also triggered the expression of inflammatory and oxidative markers in rd10 retinas. Finally, an HFD caused gut microbiome dysbiosis, increasing the abundance of potentially proinflammatory bacteria. Thus, HFD feeding drives the pathological processes of retinal degeneration by promoting oxidative stress and activating inflammatory-related pathways. Our findings suggest that consumption of an HFD could accelerate the progression of the disease in patients with retinal degenerative disorders.

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