Anti-Inflammatory Activity of Human Lens Crystallin Derived Peptide

2021 ◽  
Vol 18 ◽  
Author(s):  
Anuraag Muralidharan ◽  
Tenzin Tender ◽  
Pallavi K Shetty ◽  
Srinivas Mutalik ◽  
Krishna Sharma K ◽  
...  
Keyword(s):  
Free Radical ◽  
Skin Permeation ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Inflammatory Activity ◽  
Human Lens ◽  
Permeation Studies ◽  
Anti Inflammatory

Background: Inflammation has become the culmination point for several chronic diseases like skin diseases, asthma, neurological disorders, cancer and cardiovascular disorders. Mini αA-crystallin peptide, identified from a highly conserved region of human lens protein αA-crystallin, is known to have a chaperone-like function, hence has generated interest in exploring the anti-inflammatory potential of the peptide. Objective: The objective of the study was to evaluate anti-inflammatory potential of mini αA chaperone using in vitro, ex-vivo and in vivo models. Methods: The peptide was tested for its phosphodiestarase4 B inhibition, anti-inflammatory and free radical scavenging abilities in HaCat cells. Carbopol gel formulations with varying concentrations of mini αA-crystallin peptide and diclofenac sodium were prepared and optimized. Skin permeation studies of prepared formulations were carried out on excised abdominal skin of Wistar rat using a vertical type diffusion cell. Carrageenan induced rat paw oedema model was used for determining the anti-inflammatory potential of the peptide in prepared gel formulation with or without diclofenac sodium. Results: The peptide exhibited appreciable free radical scavenging and weak PDE4B inhibition. Gel formulation with 1% Tween-80, 1% carbopol and 10% ethanol showed better permeation compared to other formulations. The in vitro skin permeation studies revealed good improvement in permeation characteristics of diclofenac and peptide from the gels. The peptide was retained within the skin tissue, which is an ideal requirement for the delivery of anti-inflammatory topical formulation. In preclinical anti-inflammatory studies, gel formulation containing mini αA-peptide and diclofenac sodium showed a significant decrease in paw volume compared to other combinations tested. Conclusion: The study revealed the additive effect in anti-inflammatory activity by combining mini-αA peptide and diclofenac sodium which effectively reduced the inflammation.

scholarly journals Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6251
Author(s):  
Ravi Sahukari ◽  
Jyothi Punabaka ◽  
Shanmugam Bhasha ◽  
Venkata Subbaiah Ganjikunta ◽  
Shanmugam Kondeti Ramudu ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
In Vivo Studies ◽  
Root Extract ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Acalypha Indica

In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and fenofibrate (0.711%) are the predominant phytomolecules. Evidence from in vitro studies revealed that AIRME scavenges DPPH and hydroxyl radicals in a concentration dependent manner (10–50 μg/mL). Similarly, hydrogen peroxide and lipid peroxidation were also remarkably inhibited by AIRME as concentration increases (20–100 μg/mL). In vitro antioxidant activity of AIRME was comparable to ascorbic acid treatment. For in vivo studies, carrageenan (1%, sub-plantar) was injected to rats to induce localized inflammation. Acute inflammation was represented by paw-edema, and significantly elevated (p < 0.05) WBC, platelets and C-reactive protein (CRP). However, AIRME pretreatment (150/300 mg/kg bodyweight) significantly (p < 0.05) decreased edema volume. This was accompanied by a significant (p < 0.05) reduction of WBC, platelets and CRP with both doses of AIRME. The decreased activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in paw tissue were restored (p < 0.05 / p < 0.01) with AIRME in a dose-dependent manner. Furthermore, AIRME attenuated carrageenan-induced neutrophil infiltrations and vascular dilation in paw tissue. For the first time, our findings demonstrated the potent antioxidant and anti-inflammatory properties of AIRME, which could be considered to develop novel anti-inflammatory drugs.

scholarly journals In vitro screening of antioxidant, free radical scavenging and antimicrobial potential of Micrococca mercurialis whole plant extracts

2019 ◽  
Vol 10 (4) ◽  
pp. 3251-3262
Author(s):  
Thendral Hepsibha B ◽  
Gayathri Devi R ◽  
Lijitha L
Keyword(s):  
Free Radical ◽  
Plant Extracts ◽  
Skin Diseases ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Whole Plant ◽  
Anti Inflammatory ◽  
Scientific Validation

Micrococca mercurialis is widely used for the treatment of sores, skin diseases, cold, fever, rheumatic pain, and infections. The present study aimed to study the antioxidant, free radical scavenging and antibacterial potential different whole plant extracts (aqueous, ethanol, petroleum ether) of Micrococca mercurialis by in vitro methods. The amount of phenols, tannins, flavonoids and Vitamin C were estimated by conventional methods. Free radical scavenging potential was assessed by DPPH and FRAP assays. HRBC membrane-stabilizing study was done to determine its anti-inflammatory property by in vitro method. Activity against Staphylococcus aureus and Bacillus subtilis were determined in Micrococca mercurialis extracts. The phytoconstituents in the extracts of Micrococca mercurialis were explored by GC-MS analysis. The extracts of Micrococca mercurialis exhibited varied in vitro antioxidant, free radical scavenging, antibacterial and anti-inflammatory activities. The GC–MS results confirmed the presence of twenty-nine phytoconstituents totally responsible for the biological activities of Micrococca mercurialis. This pilot study has provided a scientific validation for the folkloric use of Micrococca mercurialis against many infections and diseases.

Novel saponin and benzofuran isoflavonoid with in vitro anti-inflammatory and free radical scavenging activities from the stem bark of Pterocarpus erinaceus (Poir)

2018 ◽  
Vol 28 ◽  
pp. 69-75 ◽  
Author(s):  
Paul Djouonzo Toukam ◽  
Maurice Fotsing Tagatsing ◽  
Lauve Rachel Tchokouaha Yamthe ◽  
Gakul Baishya ◽  
Nabin Chandra Barua ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Pterocarpus Erinaceus ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Activities
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