Qualitative and Quantitative Analysis of Phosphoproteomic Experimental Workflow Based on Phosphoprotein Enrichment Strategy and Two- Dimensional Difference Gel Electrophoresis (2D-DIGE) Techniques

2015 ◽  
Vol 11 (4) ◽  
pp. 252-263
Author(s):  
Zhongmin Tian ◽  
Yu Wang ◽  
Chenyang Zhao ◽  
Tianshu Wang ◽  
Entai Hou ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Qualitative And Quantitative Analysis ◽  
2D Dige ◽  
Qualitative And Quantitative ◽  
Difference Gel Electrophoresis ◽  
Phosphoprotein Enrichment ◽  
Enrichment Strategy

scholarly journals Proteomic analysis of ovomucoid hypersensitivity in mice by two-dimensional difference gel electrophoresis (2D-DIGE)

2007 ◽  
Vol 45 (12) ◽  
pp. 2372-2380 ◽  
Author(s):  
D.J. Hobson ◽  
P. Rupa ◽  
G.J. Diaz ◽  
H. Zhang ◽  
M. Yang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
2D Dige ◽  
Difference Gel Electrophoresis

scholarly journals Calreticulin as A Novel Potential Metastasis-Associated Protein in Myxoid Liposarcoma, as Revealed by Two-Dimensional Difference Gel Electrophoresis

Proteomes ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 13
Author(s):  
Takashi Tajima ◽  
Fusako Kito ◽  
Akihiko Yoshida ◽  
Akira Kawai ◽  
Tadashi Kondo
Keyword(s):  
Gel Electrophoresis ◽  
Expression Profiles ◽  
Myxoid Liposarcoma ◽  
Clinical Applications ◽  
Two Dimensional ◽  
2D Dige ◽  
Tumor Tissues ◽  
Difference Gel Electrophoresis ◽  
Protein Expression Profiles

Myxoid liposarcoma (MLS) is a mesenchymal malignancy. To identify innovate seeds for clinical applications, we examined the proteomes of primary tumor tissues from 10 patients with MLS with different statuses of postoperative metastasis. The protein expression profiles of tumor tissues were created, and proteins with differential expression associated with postoperative metastasis were identified by two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry. The validation was performed using specific antibodies and in vitro analyses. Using 2D-DIGE, we observed 1726 protein species and identified proteins with unique expression levels in metastatic MLS. We focused on the overexpression of calreticulin in metastatic MLS. The higher expression of calreticulin was confirmed by Western blotting, and gene silencing assays demonstrated that reduced expression of calreticulin inhibited cell growth and invasion. Our findings suggested the important roles of calreticulin in MLS metastasis and supported its potential utility as a prognostic biomarker in MLS. Further investigations of the functional properties of calreticulin and other proteins identified in this study will improve our understanding of the biology of MLS and facilitate novel clinical applications.

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