Antifungal features and properties of chitosan/sandalwood oil Pickering emulsion coating stabilized by appropriate cellulose nanofiber dosage for fresh fruit application

A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% sandalwood oil (SEO). Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion. We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006–0.21% CNFs); (3) stable emulsion (0.24–0.31% CNFs); and (4) regular emulsion with the addition of surfactant. Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology. Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating stabilized with CNFs (CS-SEOpick) and CS or CS-SEO was tested. The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes). The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength. Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation. Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.

The Assessment of Quality of Products Called Sandalwood Oil Based on the Information Provided by Manufacturer of the Oil on Polish, German, and English Websites

Background. Sandalwood oil is one of the most valuable raw materials worldwide. As a highly valued product, it has its own regulations based on the ISO 3518 standard, which clearly informs producers, distributors, and consumers of the requirements to be met. The aim of this study was to assess the quality of products called sandalwood oil based on the information provided by the manufacturer of the oils on Polish, German, and English websites. Methods. A Google search was utilized to collect data on sandalwood oil offered by producers and distributors in Polish and foreign markets. Information from 50 websites in each of the aforementioned languages, including the description of sandalwood oil properties on websites, method for using it, safety limitations, and presence of a product description consistent with the INCI recommendations, was gathered using Microsoft Excel software and was analyzed. The information that enabled us to estimate the quality of the oils was the botanical name of the oil-bearing plant and the price. Good-quality oils were considered to be oils with the botanical name Santalum album in the description and with a price not considerably less than the price of white sandalwood oils sold by reliable distributors who control the quality of the oils by chromatography. Ultimately, the lower price limit for one milliliter of the oil was established as PLN 21. Results and Conclusions. Good-quality sandalwood oils derived from the Santalum album plant at a price equal to or greater than the chromatographically tested items amounted to a negligible percentage of products sold online. Without knowing the botanical name of the essential oil plant and the price range of unadulterated sandalwood oil, the likelihood of buying a reliable product is low on all of the analyzed websites, with the lowest probability being observed on the Polish websites.

Antifungal Features and Properties of Chitosan/Sandalwood Oil Pickering Emulsion Coating Stabilized by Appropriate Cellulose Nanofiber Dosage for Fresh Fruit Application

A novel composite edible coating film was developed from 0.8% chitosan (CS) and 0.5% n sandalwood oil (SEO). Cellulose nanofibers (CNFs) were used as a stabilizer agent of oil-in-water Pickering emulsion. We found four typical groups of CNF level-dependent emulsion stabilization, including (1) unstable emulsion in the absence of CNFs; (2) unstable emulsion (0.006–0.21% CNFs); (3) stable emulsion (0.24–0.31% CNFs); and (4) regular emulsion with the addition of surfactant. Confocal laser scanning microscopy was performed to reveal the characteristics of droplet diameter and morphology. Antifungal tests against Botrytis cinerea and Penicillium digitatum, between emulsion coating stabilized with CNFs (CS-SEOpick) and CS or CS-SEO was tested. The effective concentration of CNFs (0.24%) may improve the performance of CS coating and maintain CS-SEO antifungal activity synergistically confirmed with a series of assays (in vitro, in vivo, and membrane integrity changes). The incorporation of CNFs contributed to improve the functional properties of CS and SEO-loaded CS including light transmission at UV and visible light wavelengths and tensile strength. Atomic force microscopy and scanning electron microscopy were employed to characterize the biocompatibility of each coating film formulation. Emulsion-CNF stabilized coating may have potential applications for active coating for fresh fruit commodities.

Antioxidant and Anti-Aging Potential of Indian Sandalwood Oil against Environmental Stressors In Vitro and Ex Vivo

Distilled from the heartwood of Santalum album, Indian sandalwood oil is an essential oil that historically has been used as a natural active ingredient in cosmetics to condition and brighten the skin. It has been documented to exhibit antioxidant, anti-inflammatory, and anti-proliferative activities. Here, we investigated the protective and anti-aging effects of Indian sandalwood oil in scavenging reactive oxygen species (ROS) in HaCaT cells and in human skin explants after exposure to oxidative stress. Using a probe DCFH-DA, the antioxidant capacity of Indian sandalwood oil was monitored following exposure to blue light at 412 nm and 450 nm or cigarette smoke. The anti-aging effect of sandalwood oil was also explored in human skin explants via the assessment of collagenase level (MMP-1). We reported that Indian sandalwood oil possessed antioxidant potential that can scavenge the ROS generated by a free radical generating compound (AAPH). Subsequent exposure to environmental stressors revealed that Indian sandalwood oil possessed superior antioxidant activity in comparison to vitamin E (alpha tocopherol). Using human skin explants, this study demonstrated that Indian sandalwood oil can also inhibit the pollutant-induced level of MMP-1. The findings indicated that Indian sandalwood oil can potentially serve as a protective and anti-aging active ingredient in cosmetics and dermatology against environmental stressors.

The Extraction THE EXTRACTION OF SANDALWOOD OIL USING THE MICROWAVE HYDRODISTILLATION METHOD WITH VARIATION OF MASS AND MATERIAL TREATMENT

The extraction of sandalwood oil using the microwave hydrodistillation method can be another alternative in the process of extracting sandalwood to obtain and improve the quality of the sandalwood oil produced. This research aims to determine the appropriate formula from the mass variation of the material and the treatment of sandalwood powder in obtaining high yield and good oil quality. The method of making sandalwood oil used is the Microwave Hydrodistillation method. From the results of this reseacrh, the best yield calculation results in the variation of the mass of 200 grams of material with dry wind treatment. Based on the density test, the result is 0.98 and the acid number is 0.6732.

A Comparison of the Composition of Selected Commercial Sandalwood Oils with the International Standard

Sandalwood oils are highly desired but expensive, and hence many counterfeit oils are sold in high street shops. The study aimed to determine the content of oils sold under the name sandalwood oil and then compare their chromatographic profile and α- and β santalol content with the requirements of ISO 3518:2002. Gas chromatography with mass spectrometry analysis found that none of the six tested “sandalwood” oils met the ISO standard, especially in terms of α-santalol content. Only one sample was found to contain both α- and β-santalol, characteristic of Santalum album. In three samples, valerianol, elemol, eudesmol isomers, and caryophyllene dominated, indicating the presence of Amyris balsamifera oil. Another two oil samples were found to be synthetic mixtures: benzyl benzoate predominating in one, and synthetic alcohols, such as javanol, polysantol and ebanol, in the other. The product label only gave correct information in three cases: one sample containing Santalum album oil and two samples containing Amyris balsamifera oil. The synthetic samples described as 100% natural essential oil from sandalwood are particularly dangerous and misleading to the consumer. Moreover, the toxicological properties of javanol, polysantol and ebanol, for example, are unknown.