scholarly journals Correlation of population SARS-CoV-2 cycle threshold values to local disease dynamics: An exploratory observational study (Preprint)

10.2196/28265 ◽  
2021 ◽  
Author(s):  
Chak Foon Tso ◽  
Anurag Garikipati ◽  
Abigail Green-Saxena ◽  
Qingqing Mao ◽  
Ritankar Das
Keyword(s):  
Observational Study ◽  
Disease Dynamics ◽  
Threshold Values ◽  
Cycle Threshold ◽  
Local Disease

scholarly journals SARS-CoV-2 Viral Load, IFNλ Polymorphisms and the Course of COVID-19: An Observational Study

2020 ◽  
Vol 9 (10) ◽  
pp. 3315
Author(s):  
Emanuele Amodio ◽  
Rosaria Maria Pipitone ◽  
Stefania Grimaudo ◽  
Palmira Immordino ◽  
Carmelo Massimo Maida ◽  
...  
Keyword(s):  
Viral Load ◽  
Observational Study ◽  
Lower Risk ◽  
Nucleotide Polymorphisms ◽  
Threshold Values ◽  
Single Nucleotide ◽  
Cycle Threshold ◽  
Viral Loads ◽  
The Relationship ◽  
Multiorgan Disease

The course of SARS-CoV-2 infection ranges from asymptomatic to a multiorgan disease. In this observational study, we investigated SARS-CoV-2 infected subjects with defined outcomes, evaluating the relationship between viral load and single nucleotide polymorphisms of genes codifying for IFNλs (interferon). The study enrolled 381 patients with laboratory-confirmed SARS-CoV-2 infection. For each patient, a standardized form was filled including sociodemographic variables and clinical outcomes. The host’s gene polymorphisms (IFNL3 rs1297860 C/T and INFL4 rs368234815 TT/ΔG) and RtReal-Time PCR cycle threshold (PCR Ct) value on SARS-CoV-2 were assessed on nasal, pharyngeal or nasopharyngeal swabs. Higher viral loads were found in patients aged > 74 years and homozygous mutant polymorphisms DG in IFNL4 (adj-OR = 1.16, 95% CI = 1.01–1.34 and adj-OR = 1.24, 95% CI = 1.09–1.40, respectively). After adjusting for age and sex, a statistically significantly lower risk of hospitalization was observed in subjects with higher RtReal-Time PCR cycle threshold values (adj-OR = 0.95, 95% CI = 0.91, 0.99; p = 0.028). Our data support the correlation between SARS-CoV-2 load and disease severity, and suggest that IFNλ polymorphisms could affect the ability of the host to modulate viral infection without a clear impact on the outcome of COVID-19.

scholarly journals Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?

ACS Omega ◽  
2021 ◽  
Author(s):  
Ilka Engelmann ◽  
Enagnon Kazali Alidjinou ◽  
Judith Ogiez ◽  
Quentin Pagneux ◽  
Sana Miloudi ◽  
...  
Keyword(s):  
Real Time ◽  
Test Results ◽  
Threshold Values ◽  
Rt Pcr ◽  
Cycle Threshold

scholarly journals KI and WU Polyomavirus in Respiratory Samples of SARS-CoV-2 Infected Patients

2021 ◽  
Vol 9 (6) ◽  
pp. 1259
Author(s):  
Carla Prezioso ◽  
Ugo Moens ◽  
Giuseppe Oliveto ◽  
Gabriele Brazzini ◽  
Francesca Piacentini ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Healthcare Workers ◽  
Respiratory Pathogens ◽  
Threshold Values ◽  
Cycle Threshold ◽  
Viral Interference ◽  
Disease Outcomes ◽  
Global Pandemic ◽  
Wu Polyomavirus ◽  
Washington University

Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) has been declared a global pandemic. Our goal was to determine whether co-infections with respiratory polyomaviruses, such as Karolinska Institutet polyomavirus (KIPyV) and Washington University polyomavirus (WUPyV) occur in SARS-CoV-2 infected patients. Oropharyngeal swabs from 150 individuals, 112 symptomatic COVID-19 patients and 38 healthcare workers not infected by SARS-CoV-2, were collected from March 2020 through May 2020 and tested for KIPyV and WUPyV DNA presence. Of the 112 SARS-CoV-2 positive patients, 27 (24.1%) were co-infected with KIPyV, 5 (4.5%) were positive for WUPyV, and 3 (2.7%) were infected simultaneously by KIPyV and WUPyV. Neither KIPyV nor WUPyV DNA was detected in samples of healthcare workers. Significant correlations were found in patients co-infected with SARS-CoV-2 and KIPyV (p < 0.05) and between SARS-CoV-2 cycle threshold values and KIPyV, WUPyV and KIPyV and WUPyV concurrently detected (p < 0.05). These results suggest that KIPyV and WUPyV may behave as opportunistic respiratory pathogens. Additional investigations are needed to understand the epidemiology and the prevalence of respiratory polyomavirus in COVID-19 patients and whether KIPyV and WUPyV could potentially drive viral interference or influence disease outcomes by upregulating SARS-CoV-2 replicative potential.

scholarly journals Cycle Threshold Values in the Context of Multiple RT-PCR Testing for SARS-CoV-2

2021 ◽  
Vol Volume 14 ◽  
pp. 1311-1317
Author(s):  
Daniel Romero-Alvarez ◽  
Daniel Garzon-Chavez ◽  
Franklin Espinosa ◽  
Edison Ligña ◽  
Enrique Teran ◽  
...  
Keyword(s):  
Threshold Values ◽  
Rt Pcr ◽  
Cycle Threshold

scholarly journals Association between Xpert MTB/RIF cycle threshold values and sputum smear microscopy in patients with pulmonary tuberculosis

2021 ◽  
pp. e20200549
Author(s):  
Gabriela Carpin Pagano1 ◽  
Giovana Rodrigues Pereira1,2 ◽  
Karen Gomes D'Ávila3 ◽  
Luciana Rott Monaiar3 ◽  
Denise Rossato Silva1,3,4
Keyword(s):  
Pulmonary Tuberculosis ◽  
Sputum Smear ◽  
Smear Microscopy ◽  
Threshold Values ◽  
Sputum Smear Microscopy ◽  
Cycle Threshold

scholarly journals DO XPERT MTB/RIF CYCLE THRESHOLD VALUES PROVIDE INFORMATION ABOUT PATIENT DELAYS FOR TUBERCULOSIS DIAGNOSIS?

2017 ◽  
Vol 2 (Suppl 2) ◽  
pp. A45.2-A45
Author(s):  
Willy Ssengooba ◽  
Durval Respeito ◽  
Edson Mambuque ◽  
Silvia Blanco ◽  
Inacio Mandomando ◽  
...  
Keyword(s):  
Threshold Values ◽  
Cycle Threshold ◽  
Tuberculosis Diagnosis
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