scholarly journals Morphofunctional characterization of rat thymus mast cells after administration of magnesium orotate mechanically activated forms

2021 ◽  
Vol 25 (3) ◽  
pp. 248-255
Author(s):  
Natalya N. Chuchkova ◽  
Marina V. Smetanina ◽  
Alexei E. Shklyaev ◽  
Ksenia A. Pazinenko ◽  
Natalya V. Kormilina ◽  
...  
Keyword(s):  
Magnesium Deficiency ◽  
Argon Plasma ◽  
Atomic Emission ◽  
Immunomodulatory Activity ◽  
Drug Induced ◽  
Inductively Coupled ◽  
The People ◽  
Thymus Tissue ◽  
Magnesium Orotate

Relevance. The topicality of the work is determined by the wide spread of hypomagnesemia among the people, which makes it necessary to correct it. The aim of the work is to elucidate the cell-mediated response of the thymus mastocytic link to magnesium deficiency and its correction by the mechanoactivated form of magnesium orotate. Materials and Methods . Animals with drug-induced magnesium deficiency (administration of furosemide 30 mg/kg for 14 days) were administered either the initial preparation Magnerot (Magnerot, Vervag Pharma, Germany), or its mechanoactivated form. The level of magnesium in the blood was determined by test systems ARKREY (Japan). The concentration of magnesium in the thymus tissue was determined by the method of emission spectroscopy with inductively coupled (argon) plasma on an atomic emission spectrometer. Density of mastocytes and the indices of degranulation and granulolosis were calculated on paraffin sections of the thymus after coloration with toluidine blue. Results and Discussion . It was shown that furosemide administration the amount of magnesium decreased in the blood (from 1,750,08 to 0,9020,18 mmol/l, p0,05), but increased in the thymus (from 1,60,6 in the control to 3,71,2 mg/l); in the gland tissue, the number of mastocytes of morphotype A decreased and the number of mastocytes of morphotype D, after active degranulation, increased (by 7,1 times, p0,05). The type of mastocyte secretion in hypomagnesemia is represented by the merocrine variant. The administration of the initial magnesium orotate led to an increase in the concentration of magnesium in the blood to 1,150,25 mmol/l, which is 65,7% of the initial level, the amount of magnesium in the thymus remained elevated (3,41,1 mg/l), the number of actively degranulating cells (morphotype D) was increased. Mechanoactivated magnesium orotate restored the concentration of Mg2+ in the blood to 89,1% (1,560,18 mmol/l, p0,05) and decreased in the thymus (to 2,30,7 mg/l), restored the subpopulation of mastocytes saturated with heparin (type A), reduced the number of mastocytes of morphotype D. Conclusion . The mechanoactivated form of magnesium orotate has a normalizing effect on the population of thymic mastocytes, shows pronounced immunomodulatory activity, which allows us to consider it as a potential therapeutic agent for clinical testing in the complex therapy of hypomagnesemia and associated immunodeficiency.

scholarly journals THE RESULTS OF THE MONITORING OF THE MERCURY CONTAMINATION WITHIN A MEGAPOLIS

2018 ◽  
Vol 97 (12) ◽  
pp. 1189-1194 ◽  
Author(s):  
A. M. Malov ◽  
L. V. Lukovnikova ◽  
Liliya A. Alikbayeva ◽  
I. S. Iakubova ◽  
D. K. Shchegolikhin
Keyword(s):  
Soil Layer ◽  
Argon Plasma ◽  
Atomic Emission ◽  
Mercury Content ◽  
Mercury Contamination ◽  
Human Organism ◽  
Professional Activities ◽  
Inductively Coupled ◽  
Mercury Compounds ◽  
Environmental Objects

Introduction. Macromycetes include mercury compounds in their metabolism processes. The method for assessing the contamination of the soil layer (topsoil) by mercury by estimating the mercury content in the mushrooms is accessible and sufficiently informative. Mercury is a persistent inorganic ecotoxicant, it enters the human body from the environment. This process requires constant monitoring of the content of this hazardous metal in the environment and the in biomaterials of the human organism. Material and methods. The object of the study was selected bracket macromycetes growing on open soil areas: lawns, boulevards, parks, squares, etc. There were studied representatives from the fungal families as follows: Agaricacea, Boletaceae, Russu-laceae, Coprinaceae. Mushrooms were collected in the stage of sporulation, in the spring-summer-autumn period from 2002 to 2017. The determination of mercury in fungi was performed by atomic absorption method on specialized mercury analyzers of the “Julia” series (detection limit of 1 ng/g, the error of the method is not more than 15%). Mercury content in hair was determined in 1153 St. Petersburg residents aged from 0 to 80 years using atomic emission and mass spectrometry methods with inductively coupled argon plasma on devices Elan 9000 (Perkin Elmer, USA) and Optima 2000 V (Perkin Elmer, USA). Results. The results of the analysis of mushrooms collected in 2017 on one of the green streets of the industrial district of Saint-Petersburg demonstrate a high degree of mercury contamination of the megapolis. Mushrooms (Cinereus comatus) were collected at three locations of Alameda, separated by approximately 100 m, and were analyzed for the content of mercury in them. The following values of mercury in mushrooms were got is a 0.61 mg/kg, 0.83 mg/kg and 0.35 mg/kg. The hair of 1153 inhabitants of St.-Petersburg has been analyzed for mercury content. The highest concentrations of mercury have been established to be set at working population aged 18-64 years. This fact can be explained by the professional activities associated with the contact with mercury and active way of life, particularly with greater frequency of the use in the diet of mushrooms and other products, being potential sources of mercury. Discussion. The performed studies show the mercury concentration to be not only generalized but also stable. The pollution of the territory of St. Petersburg is little different from the pollution of the territories of other cities, where the mercury content in mushrooms is also found to be high in comparison with the permissible level. Conclusion. The obtained data indicate a high stable contamination of the territory of St. Petersburg and its nearest suburbs with mercury. Mercury and its compounds in environmental objects (mushrooms) can present the real danger to the health of the population, therefore there is a need for non-invasive monitoring of the content of mercury in biomaterials of the human organism and environmental objects.

Cold-Vapor Generation for Inductively Coupled Argon Plasma/Atomic Emission Spectrometric Analysis. Part 3. Mercury

1994 ◽  
Vol 77 (2) ◽  
pp. 473-480 ◽  
Author(s):  
Kim A Anderson ◽  
Brandon Isaacs ◽  
Mark Tracy ◽  
Gregory Möller
Keyword(s):  
Detection Limit ◽  
Argon Plasma ◽  
Atomic Emission ◽  
Plasma Atomic Emission ◽  
Vapor Generation ◽  
Test Portion ◽  
Cold Vapor ◽  
Inductively Coupled ◽  
Drinking Water Standard ◽  
Cold Vapor Generation

Abstract A method is presented for the determination of total mercury in environmental samples by inductively coupled argon plasma/atomic emission spectroscopy (ICP/AES) using cold-vapor generation. A 1.0 g or 10 mL test portion is digested 6–12 h in a 16 × 150 mm, 10 mL volumetric test tube at room temperature and is subsequently digested on a programmed heating block at 95°C with trace-metal grade nitric acid. The digested samples are next treated with potassium permanganate and heated between 70 and 95°C; thereafter, oxalic acid is added to reach the final endpoint. The mercury(II) is reduced by sodium borohydride in a simplified continuous manifold. A standard pneumatic nebulizer effects the gas–liquid separation of mercury. Mercury is quantitated by ICP/AES at 194.232 nm. The instrument detection limit for the method is 0.2 μg/L. Water samples are analyzed directly. The detection limit is below the current National Primary Drinking Water Standard Maximum Concentration Limit of 2 μg/L for mercury. For a 10:1 dilution of a nominal 1.0 g solid test portion, the detection limit is 2.0 μg/L. This concentration is also less than the regulatory limit of 200 μg/L for the U.S. Environmental Protection Agency’s Toxicity Characteristic Leaching Procedure. The quantitation is linear up to 300 μg/kg. The method demonstrated statistical control for samples of biological and environmental interest.

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