Daerah penelitian terletak di desa Muaro Limun, Kecamatan Limun Kabupaten Sarolangun Provinsi Jambi. Sungai limun, salah satu sungai besar di daerah kabupaten sarolangun yang dimanfaatkan oleh mayarakat sekitarnya sebagai sumber penghidupan. Penelitian bertujuan untuk mengetahui pengaruh kegiatan penambangan terhadap kualitas air sungai Batang Limun, dan perubahan sifat fisik dan kimia yang diakibatkan kegiatan penambangan.Metode yang digunakan adalah metode grab sampel, serta stream sedimen untuk dianalis di laboratorium. Sejumlah sampel diambil di beberapa lokasi Penambangan Emas berdasarkan Aliran Sub-DAS dan dibandingkan dengan beberapa sampel lain yang diambil pada lokasi yang belum terkontaminasi oleh kegiatan penambangan. Analisis kualitas air mengacu pada SMEWWke 22 tahun 2012 dan standar baku mutu air kelas II dalam PP No 82 yang dikeluarkan oleh Menteri Kesehatan No. 492/Menkes/Per/IV/2010. Diketahui sungai Batang Limun telah mengalami perubahan karakteristik fisika dan kimia. Dari grafik kosentrasi kekeruhan, pH, TSS, TDS Cu, Pb, Zn, Mn, Hg terlihat bahwa penambang emas tanpa izin (PETI) dengan cara amalgamasi yang menyebabkan terjadinya penurunan kualitas air sungai. Sejak tahun 2009 sampai tahun 2015 sungai Limun dan sekitarnya terus mengalami penurunan kualitas air. Penurunan kualitas yang cukup tinggi terjadi yaitu peningkatan nilai Rata-rata konsentrasi merkuri pada sungai Batang Limun dari 0,18ppb (0,00018 mg/l) menjadi 0,3ppb (0,0003 mg/l), peningkatan tersebut dipengaruhi oleh proses kegiatan penambangan dan nilai tersebut masih dibawah standar baku mutu air kelas II pp nomor 82 tahun 2010.Kata kunci : Kualitas Air, Sungai Limun,TSS, Merkuri, PETI Limun river is one of the major rivers in the area of Sarolangun, which utilized by the society as a source of livelihood. The aim of study to analyze the effect of mining activities on the water quality of Batang Limun River, and the changes of physical and chemical properties of water. The method used are grab and stream samples to sediment analyzed in the laboratory. A number of samples were taken at several locations based Flow Gold Mining Sub-watershed and compared to some other samples taken at the location that has not been contaminated by mining activities. Water quality analysis referring to SMEWW, 22nd edition 2012 and refers to Regulation No 82 that issued by Minister of Health No. 492 / Menkes / Per / IV / 2010.The results showed that the Limun river has undergone chemical changes in physical characteristics. These symptoms can be seen from the discoloration of clear water in the river before the mine becomes brownish after mining, based on graphic of muddiness concentration: pH, TSS, TDS Cu, Pb, Zn, Mn, Hg have seen that the illegal miner which used amalgamation caused deterioration in water quality, data from 2009 to 2015 Limun river and surrounding areas continue to experience a decrease in water quality. The decreasing of water quality showed in the TSS parameter which found in the area is to high based on the standard of water quality class II pp number 82 of 2010. An increase in the value of average concentrations of mercury in the Batang Limun river before mine 0,18ppb (0.00018 mg / l) into 0,3ppb (0.0003 mg / l) on the river after the mine. The increase was affected by the mining activities and the value is still below the air quality standard Grade II pp numbers 82 years 2010, although the value is still below with the standards quality standard, the mercury levels in water should still be a major concern because if it accumulates continuously in the water levels will increase and will be bad for health. In contrast to the concentration of mercury in sediments that have a higher value is 153 ppb (0,513ppm ) .Key Words : Water Quality, Limun River, Mercury, Illegal gold mining