In order to complete the high-precision calibration of the planetary rover navigation camera using limited initial data in-orbit, we proposed a joint adjustment model with additional multiple constraints. Specifically, a base model was first established based on the bundle adjustment model, second-order radial and tangential distortion parameters. Then, combining the constraints of collinearity, coplanarity, known distance and relative pose invariance, a joint adjustment model was constructed to realize the in orbit self-calibration of the navigation camera. Given the problem of directionality in line extraction of the solar panel due to large differences in the gradient amplitude, an adaptive brightness-weighted line extraction method was proposed. Lastly, the Levenberg-Marquardt algorithm for nonlinear least squares was used to obtain the optimal results. To verify the proposed method, field experiments and in-orbit experiments were carried out. The results suggested that the proposed method was more accurate than the self-calibration bundle adjustment method, CAHVOR method (a camera model used in machine vision for three-dimensional measurements), and vanishing points method. The average error for the flag of China and the optical solar reflector was only 1 mm and 0.7 mm, respectively. In addition, the proposed method has been implemented in China’s deep space exploration missions.