scholarly journals Evidences reveal that cattle and buffalo evolutionary derived from the same ancestor based on cytogenetic and molecular markers

2006 ◽  
Vol 22 (3-4) ◽  
pp. 1-9 ◽  
Author(s):  
S.M. Abdel-Rahman
Keyword(s):  
Cytochrome B ◽  
Fragment Length ◽  
Restriction Analysis ◽  
Pcr Amplification ◽  
Gene Encoding ◽  
Ssr Analysis ◽  
Mitochondrial Cytochrome B ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Species Specific

Muscle-DNA from cattle and buffalo was extracted to amplify the mitochondrial DNA segment (cytochrome b gene) and the gene encoding species-specific repeat (SSR) region. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and SSR techniques were used to identify of species origin. Restriction analysis of PCR-RFLP of the mitochondrial cytochrome b segment and SSR analysis showed no differences between cattle and buffalo. Where, the fragment length (bp) generated by AluI PCR-RFLP were 190, 169 and PCR amplification size of the gene encoding SSR region was 603 bp in both cattle and buffalo. Consequently, finding from this study could be revealed that cattle and buffalo are evolutionary derived from the same ancestor.

scholarly journals Identification and differentiation among chicken’s, duck’s, quail’s, rabbit’s and turkey's meat using PCR-RFLP technique

2015 ◽  
Vol 31 (1) ◽  
pp. 101-108 ◽  
Author(s):  
S.M. Abdel-Rahman ◽  
A.M. Elmaghraby ◽  
A.S. Haggag
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome B ◽  
Fragment Length ◽  
Restriction Analysis ◽  
Restriction Enzymes ◽  
Pcr Product ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Species Specific ◽  
Rflp Assay

PCR-RFLP technique was developed for identification and differentiation among chicken?s, duck?s, quail?s, rabbit?s and turkey's meat. DNA from small amount of muscles (0.05 g) was extracted and a region of mitochondrial DNA (cytochrome-b gene) in chicken, duck, quail, rabbit and turkey was amplified by PCR. Fragment length of the PCR product was 371 bp in chicken, 374 bp in duck and rabbit and 377 bp in both quail and turkey. Six nucleotides different makes it difficult to differentiate among these five species-specific meat. For differentiation, three different restriction enzymes (DdeI, MspI and TaqI) were used to digest the PCR products. Restriction analysis showed difference among chicken?s, duck?s, quail?s, rabbit?s and turkey's meat. Where, DdeI yielded two fragments (291 and 83 bp) only in rabbit?s meat. MspI yielded three fragments (221, 85 and 65 bp) in chicken?s meat and two fragments (290 and 87 bp) in both quail?s and turkey's meat. TaqI yielded three fragments (146, 134 and 94 bp) in duck?s meat and two fragments (226 and 151 bp) in quail?s meat. The use of Cytb- PCR-RFLP assay allowed a direct and fast authentication and differentiation among chicken?s, duck?s, quail?s, rabbit?s and turkey's meat.

scholarly journals Determination of Cattle and Buffalo Skin Crackers Using Polymerase Chain Reaction Restriction Fragment Length Polymorphism

2018 ◽  
Vol 35 (2) ◽  
pp. 184
Author(s):  
Rulli Riana Dewi ◽  
Yuny Erwanto ◽  
Nanung Agus Fitriyanto
Keyword(s):  
Cytochrome B ◽  
Restriction Enzyme ◽  
Cytochrome B Gene ◽  
Universal Primer ◽  
Mitochondrial Cytochrome B ◽  
Pcr Rflp ◽  
Dna Mixture ◽  
Polymerase Chain ◽  
Mixture Samples

The aim of this study was to determine of cattle and buffalo species based on cytochrome b gene using PCR-RFLP. Cattle and buffalo hides were obtained from a slaughterhouse in Yogyakarta and Kudus Regency. To confirm the effectiveness and specificity of this fragment, there are seven of DNA mixture samples in various levels. Isolate DNA samples were amplified using universal primer of cytochrome b gene, then PCR amplicon was digested by RsaI restriction enzyme.. The result showed that mitochondrial cytochrome b gene successfully amplified fragments of 359 bp. RsaI restriction enzyme was able to cleave buffalo cytochrome b gene into two fragment  (326 and 23 bp), while the cytochrome b gene of the skin cattle DNA was uncleaved. . In conclusion, this study indicated that mixture DNA of cattle and buffalo hides could be digested by RsaI restriction enzyme  and determination of the buffalo hides in mixture samples could be detected into  10% level. Furthermore, RsaI enzyme could be used to specific identification buffalo species. PCR-RFLP technology has a potential and reliable method to identify  of the existence of r buffalo hides in the mixture with other hides.

Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis: A Simple Method for Species Identification in Food

1995 ◽  
Vol 78 (6) ◽  
pp. 1542-1551 ◽  
Author(s):  
Rolf Meyer ◽  
Christiane Höfelein ◽  
Jürg Lüthy ◽  
Urs Candrian
Keyword(s):  
Polymerase Chain Reaction ◽  
Species Identification ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Chain Reaction ◽  
Heat Treated ◽  
Mitochondrial Cytochrome B ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Abstract The polymerase chain reaction (PCR) technique was applied to meat species identification in marinated and heat-treated or fermented products and to the differentiation of closely related species. DNA was isolated from meat samples by using a DNA-binding resin and was subjected to PCR analysis. Primers used were complementary to conserved areas of the vertebrate mitochondrial cytochrome b (cytb) gene and yielded a 359 base-pair (bp) fragment, including a variable 307 bp region. Restriction endonuclease analysis based on sequence data of those fragments was used for diffferentiation among species. Restriction fragment length polymorphisms (RFLPs) were detected when pig, cattle, wild boar, buffalo, sheep, goat, horse, chicken, and turkey amplicons were cut with Alul, Rsal, Taql, and Hinfl. Analysis of sausages indicates the applicability of this approach to food products containing meat from 3 different species. The PCR–RFLP analytical method detected pork in heated meat mixtures with beef at levels below 1%, and the method was confirmed with porcine- and bovine-specific PCR assays by amplifying fragments of their growth hormone genes. Inter- and intraspecific differences of more than 22 animal species with nearly unknown cytb DNA sequences, including hoofed mammals (ungulates), and poultry were determined with PCR–RFLP typing by using 20 different endonucleases. This typing method allowed the discrimination of game meats, including stag, roe deer, chamois, moose, reindeer, kangaroo, springbok, and other antelopes in marinated and heat-treated products.

scholarly journals PCR amplification of species-specific repeat for meat DNA identification via genetic markers in cattle & sheep

2005 ◽  
Vol 21 (1-2) ◽  
pp. 1-12 ◽  
Author(s):  
M.M. Ahmed
Keyword(s):  
Dna Sequences ◽  
Pcr Amplification ◽  
Gene Encoding ◽  
Ssr Analysis ◽  
Sheep Meat ◽  
As Species ◽  
Heat Treated ◽  
Cattle Meat ◽  
Species Specific ◽  
Cattle And Sheep

The designed and evaluated four assays based upon PCR amplification of species-specific repeat (SSR) for detection, identification and authentication of cattle and sheep on the DNA level. SSR primers were applied in the polymerase chain reaction (PCR), the products has been used for the specific identification of cattle and sheep meat. PCR amplification size of the gene encoding SSR region in cattle and sheep meat was 603 bp and 374 bp respectively. The results showed that SSR analysis produced a pattern that allowed a direct identification of cattle and sheep meat in raw and heat-treated samples. Also the results showed that SSR analysis provided with a rapid and effective method to detect the meat species and it could be easily identified and authenticated. In addition, the SSR PCR methods, as species-specific, are highly sensitive and will improve the detection limits for DNA sequences derived from these species. Also indirect application is the detection of disease cow (cattle) lunacy by PCR methods for authentication of cow (cattle) meat from origin country.

scholarly journals Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

2000 ◽  
Vol 66 (10) ◽  
pp. 4340-4344 ◽  
Author(s):  
T. Deak ◽  
J. Chen ◽  
L. R. Beuchat
Keyword(s):  
Yarrowia Lipolytica ◽  
Rapd Analysis ◽  
Restriction Analysis ◽  
Pcr Amplification ◽  
Yeast Cells ◽  
Chromosomal Dna ◽  
Intergenic Sequences ◽  
Species Specific ◽  
Candida Zeylanoides ◽  
Its Pcr

ABSTRACT Yeast isolates from raw and processed poultry products were characterized using PCR amplification of the internally transcribed spacer (ITS) 5.8S ribosomal DNA region (ITS-PCR), restriction analysis of amplified products, randomly amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE). ITS-PCR resulted in single fragments of 350 and 650 bp, respectively, from eight strains of Yarrowia lipolytica and seven strains of Candida zeylanoides. Digestion of amplicons with HinfI andHaeIII produced two fragments of 200 and 150 bp fromY. lipolytica and three fragments of 350, 150, and 100 bp from C. zeylanoides, respectively. Although these fragments showed species-specific patterns and confirmed species identification, characterization did not enable intraspecies typing. Contour-clamped heterogeneous electric field PFGE separated chromosomal DNA of Y. lipolytica into three to five bands, most larger than 2 Mbp, whereas six to eight bands in the range of 750 to 2,200 bp were obtained from C. zeylanoides. Karyotypes of both yeasts showed different polymorphic patterns among strains. RAPD analysis, using enterobacterial repetitive intergenic sequences as primers, discriminated between strains within the same species. Cluster analysis of patterns formed groups that correlated with the source of isolation. For ITS-PCR, extraction of DNA by boiling yeast cells was successfully used.

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