Probiotic potential of Lactobacillus fermentum G-4 originating from the meconium of newborns

The present study was dedicated to determining probiotic potential of a human isolate G-4, originated from meconium. The isolate was identified using morphological, physiological and biochemical assays and molecular method based on 16S rRNA gene sequencing. In order to evaluate its probiotic properties in vitro tests were performed: the survival in simulated gastrointestinal conditions, adhesion to hexadecane, and antimicrobial activity. Safety aspects of the isolate were examined by testing toxicity, gastrointestinal tolerance and bacterial translocation in vivo, as well as hemolytic activity in vitro. The isolate G-4, identified as Lactobacillus fermentum, showed viability in artificial gastric and intestinal juice (low degree of cell viability reduction for 0.69 and 1.30 logCFU mL-1 units, respectively), moderate adhesion to hexadecane (39?2.1 %), and antimicrobial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serotype Abony and Clostridium sporogenes, due to production of lactic acid (9.80 g L-1). No signs of toxicity, bacterial translocation, hemolytic activity, were observed.

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Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

BMC Microbiology ◽

10.1186/s12866-021-02264-5 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Nathan das Neves Selis ◽

Hellen Braga Martins de Oliveira ◽

Hiago Ferreira Leão ◽

Yan Bento dos Anjos ◽

Beatriz Almeida Sampaio ◽

...

Keyword(s):

Antimicrobial Activity ◽

Neisseria Gonorrhoeae ◽

Clinical Importance ◽

Gardnerella Vaginalis ◽

Haemolytic Activity ◽

Probiotic Properties ◽

Vaginal Infections ◽

Probiotic Potential

Abstract Background Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. Results The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. Conclusion The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

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Safety and efficacy of human breast milk Lactobacillus fermentum CECT 5716. A mini-review of studies with infant formulae

Beneficial Microbes ◽

10.3920/bm2014.0091 ◽

2015 ◽

Vol 6 (2) ◽

pp. 219-224 ◽

Cited By ~ 10

Author(s):

E. López-Huertas

Keyword(s):

Breast Milk ◽

Probiotic Strain ◽

Lactobacillus Fermentum ◽

Controlled Trials ◽

Human Breast Milk ◽

Probiotic Properties ◽

Human Breast ◽

Safety And Efficacy

Human breast milk has been described as a source of lactic acid bacteria. Lactobacillus fermentum CECT5716 is a human breast milk strain whose probiotic properties, safety and efficacy has been demonstrated in vitro and in vivo, including controlled trials with human adults. Since the origin of this probiotic strain is human breast milk, we aimed to investigate the safety and efficacy of an infant and a follow-on formulas supplemented with this strain of L. fermentum. We carried out two randomised controlled trials: one trial with infants of 6-12 months of age (follow-on formula study) and another one with infants from 1 to 5 months of age (infant formula study). The results from the trials showed that the probiotic formulas were safe, well tolerated and might be useful for the prevention of community-acquired infections.

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Antimicrobial activities and in vitro properties of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes of high latitude water areas in Xinjiang, China

10.21203/rs.2.12860/v2 ◽

2019 ◽

Author(s):

Xiaojing Wei ◽

Yan Zhang ◽

Hong Zhou ◽

Fengwei Tian ◽

Yongqing Ni

Keyword(s):

Antimicrobial Activity ◽

Salmonella Enterica ◽

Intestinal Tract ◽

Cold Water ◽

In Vitro Tests ◽

Rrna Gene ◽

Food Preservative ◽

Cold Adapted ◽

Wide Range

Abstract Background There are still a large variety of microorganisms among aquatic animals, especially probiotic lactic acid bacteria in cold water fishes at high latitudes have not been fully developed. Hence, the present study aims to evaluate the probiotic potential of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes (Xinjiang) and select candidates to be used as new food preservative agents and/or probiotic additives in feeding of aquaculture. Results A total of 43 Lactobacillus spp. were isolated from 16 kinds of intestinal tract of cold-water fishes. They were characterized by phenotypic methods, identified using Rep-PCR and 16S rRNA gene sequencing as four species: Lactobacillus sakei (22 isolates), Lactobacillus plantarum (16 isolates), Lactobacillus casei (4 isolates) and Lactobacillus paracasei (1 isolate). The in vitro tests included survival in low pH and bile, antimicrobial activity (against Escherichia coli , Salmonella enterica subsp. enterica serovar Typhimurium , Salmonella enterica subsp. enterica , Listeria monocytogenes , and Listeria innocua ), resistance to 15 antibiotics and hemolytic tests. Among all 43 lactobacilli isolates, the 22 isolates showed a wide range of antimicrobial activity against 6 different pathogenic strains. There were twenty isolates growing at a optimal temperature ranging 16~20°C, which were initially considered to be cold-adapted strains. Two (2) Lb. sakei strains and 2 Lb. plantarum strains demonstrated the highest survivability after 4 h of exposure at pH 2.0. Most of the tested strains cannot be cultured after exposed into 0.5% bile solution for 4 h, while 2 Lb. plantarum strains (E-HLM-3, CQ-CGC-2) and 1 Lb. sakei strain M-DGM-2 survived even at 2% bile concentration. In addition, the safety assessment showed that 22 strains without any detectable hemolytic activity and resistant to glycopeptides (vancomycin, teicoplanin), levofloxacin, aztreonam, amikacin and oxacillin, while all the studied lactobacilli showed sensitivity to or semi-tolerant to other antibiotics. Conclusions Based on all the experiments, 3 strains, including E-HLM-3, CQ-CGC-2, and M-DGM-2 might be a candidate of choice for using in the food preservative agents and/or probiotic additives in feeding of aquaculture.

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Probiotic Potential and Safety Properties ofLactobacillus plantarumfrom Slovak Bryndza Cheese

BioMed Research International ◽

10.1155/2013/760298 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 23

Author(s):

Anna Belicová ◽

Mária Mikulášová ◽

Roman Dušinský

Keyword(s):

Antimicrobial Activity ◽

Lactobacillus Plantarum ◽

Bacterial Pathogens ◽

Lactobacillus Fermentum ◽

Galactosidase Activity ◽

Inhibition Activity ◽

Strong Inhibition ◽

Safety Properties ◽

Probiotic Potential

One hundred and twenty-five acid-resistant presumptive lactobacilli were isolated from Slovak Bryndza cheese and screened for their antimicrobial activity against eight bacterial pathogens using spot agar assay. Out of twenty-sixLactobacillusstrains with strong inhibition activity, twenty were identified asLactobacillus plantarumand six asLactobacillus fermentum. The most active elevenL. plantarumisolates were further characterizedin vitrofor some probiotic and safety properties. Only three isolates K10, K21, and ZS07 showed the ability to grow over 50% in the presence of 0.3% bile. Strong deconjugation efficiency was determined for CK06 and K21. The highestβ-galactosidase activity was shown in isolates ZS11, B01, CK06, and ZS07. Only three of the strains had the ability to produce tyramine: CK06, LM1, and ZS11. Strains K09, K21, ZS11, and ZS15 were susceptible to all tested antibiotics. Analysis of the results confirmed theL. plantarumisolates ZS07 and K21 as the most suitable for probiotic use, due to their desirable probiotic and safety characteristics.

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Probiotic Potential ofLactobacillusStrains with Antimicrobial Activity against Some Human Pathogenic Strains

BioMed Research International ◽

10.1155/2014/927268 ◽

2014 ◽

Vol 2014 ◽

pp. 1-16 ◽

Cited By ~ 77

Author(s):

Parisa Shokryazdan ◽

Chin Chin Sieo ◽

Ramasamy Kalavathy ◽

Juan Boo Liang ◽

Noorjahan Banu Alitheen ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Activities ◽

Rrna Gene ◽

Human Pathogens ◽

Bacterial Strains ◽

Probiotic Potential ◽

Good Ability ◽

Acid And Bile Tolerance ◽

Infant Feces

The objective of this study was to isolate, identify, and characterize some lactic acid bacterial strains from human milk, infant feces, and fermented grapes and dates, as potential probiotics with antimicrobial activity against some human pathogenic strains. One hundred and forty bacterial strains were isolated and, after initial identification and a preliminary screening for acid and bile tolerance, nine of the best isolates were selected and further identified using 16 S rRNA gene sequences. The nine selected isolates were then characterizedin vitrofor their probiotic characteristics and their antimicrobial activities against some human pathogens. Results showed that all nine isolates belonged to the genusLactobacillus. They were able to tolerate pH 3 for 3 h, 0.3% bile salts for 4 h, and 1.9 mg/mL pancreatic enzymes for 3 h. They exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics. They also showed good antimicrobial activities against the tested pathogenic strains of humans, and most of them exhibited stronger antimicrobial activity than the reference strainL. caseiShirota. Thus, the nineLactobacillusstrains could be considered as potential antimicrobial probiotic strains against human pathogens and should be further studied for their human health benefits.

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Antimicrobial activities and in vitro properties of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes of high latitude water areas in Xinjiang, China

10.21203/rs.2.12860/v1 ◽

2019 ◽

Author(s):

Xiaojing Wei ◽

Yan Zhang ◽

Hong Zhou ◽

Fengwei Tian ◽

Yongqing Ni

Keyword(s):

Antimicrobial Activity ◽

Salmonella Enterica ◽

Intestinal Tract ◽

Cold Water ◽

In Vitro Tests ◽

Rrna Gene ◽

Food Preservative ◽

Cold Adapted ◽

Wide Range

Abstract Background There are still a large variety of microorganisms among aquatic animals, especially probiotic lactic acid bacteria in cold water fishes at high latitudes have not been fully developed. Hence, the present study aims to evaluate the probiotic potential of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes (Xinjiang) and select candidates to be used as new food preservative agents and/or probiotic additives in feeding of aquaculture. Results A total of 43 Lactobacillus spp. were isolated from 16 kinds of intestinal tract of cold-water fishes. They were characterized by phenotypic methods, identified using rep-PCR and 16S rRNA gene sequencing as four species: Lactobacillus sakei (22 isolates), Lactobacillus plantarum (16 isolates), Lactobacillus casei (4 isolates) and Lactobacillus paracasei (1 isolate). The in vitro tests included survival in low pH and bile, antimicrobial activity (against Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica, Listeria monocytogenes, and Listeria innocua), resistance to 15 antibiotics and hemolytic tests. Among all 43 lactobacilli iaolates, the 22 isolates showed a wide range of antimicrobial activity against 6 different pathogenic strains. There were twenty isolates growing at a optimal temperature ranging 16~20°C, which were initially considered to be cold-adapted strains. Two (2) Lb. sakei strains and 2 Lb. plantarum strains demonstrated the highest survivability after 4 h of exposure at pH 2.0. Most of the tested strains cannot be cultrued after exposed into 0.5% bile solution for 4 h, while 2 Lb. plantarum strains (E-HLM-3, CQ-CGC-2) and 1 Lb. sakei strain M-DGM-2 survived even at 2% bile concentration. In addition, the safety assessment showed that 22 strains without any detectable hemolytic activity and resistant to glycopeptides (vancomycin, teicoplanin), levofloxacin, aztreonam, amikacin and oxacillin, while all the studied lactobacilli showed sensitivity to or semi-tolerant to other antibiotics. Conclusions In conclusion, based on all the experiments, 3 strains, including E-HLM-3, CQ-CGC-2, and M-DGM-2 might be a candidate of choice for using in the food preservative agents and/or probiotic additives in feeding of aquaculture.

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Probiotic properties of an indigenous Pediococcus pentosaceus strain on Tenebrio molitor larval growth and survival

Journal of Insects as Food and Feed ◽

10.3920/jiff2020.0156 ◽

2021 ◽

pp. 1-12

Author(s):

A. Lecocq ◽

M.E. Natsopoulou ◽

I.E. Berggreen ◽

J. Eilenberg ◽

L.-H. Lau Heckmann ◽

...

Keyword(s):

Production Systems ◽

Larval Growth ◽

Amplicon Sequencing ◽

Tenebrio Molitor ◽

Rrna Gene ◽

Probiotic Properties ◽

Pediococcus Pentosaceus ◽

Growth And Survival

Optimising the production of insects for food and feed and ensuring their health are growing concerns for producers. Insects suffer from a range of insect pathogenic microorganisms, and the management of such diseases is essential. One solution is the introduction of beneficial probiotic bacteria into the diet of the insects. Here, we show that a lactic acid bacterial strain, Pediococcus pentosaceus, isolated from the gut of the mealworm, Tenebrio molitor, was able to inhibit the growth of selected insect pathogens in vitro. Using in vivo assessments of the host’s fitness benefits conferred by the lactic bacterium we show a significant effect of P. pentosaceus on larval growth rate and survival into adulthood. Gut microbiota analysis focusing on bacterial composition based on 16S rRNA gene amplicon sequencing suggests that P. pentosaceus could have successfully colonised the guts, or altered their bacteria, of the larvae that received it. Finally, we discuss our results in the context of mass insect production systems and outline the remaining work needed to explore and secure the role of beneficial bacterial additives in the field.

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Antimicrobial activities and in vitro properties of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes of high latitude water areas in Xinjiang, China

BMC Microbiology ◽

10.1186/s12866-019-1623-3 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Xiaojing Wei ◽

Yan Zhang ◽

Hong Zhou ◽

Fengwei Tian ◽

Yongqing Ni

Keyword(s):

Antimicrobial Activity ◽

Salmonella Enterica ◽

Intestinal Tract ◽

Cold Water ◽

In Vitro Tests ◽

Rrna Gene ◽

Food Preservative ◽

Cold Adapted ◽

Wide Range

Abstract Background There are still a large variety of microorganisms among aquatic animals, especially probiotic lactic acid bacteria in cold water fishes at high latitudes have not been fully developed. Hence, the present study aims to evaluate the probiotic potential of cold-adapted Lactobacillus strains isolated from the intestinal tract of cold water fishes (Xinjiang) and select candidates to be used as new food preservative agents and/or probiotic additives in feeding of aquaculture. Results A total of 43 Lactobacillus spp. were isolated from 16 kinds of intestinal tract of cold-water fishes. They were characterized by phenotypic methods, identified using Rep-PCR and 16S rRNA gene sequencing as four species: Lactobacillus sakei (22 isolates), Lactobacillus plantarum (16 isolates), Lactobacillus casei (4 isolates) and Lactobacillus paracasei (1 isolate). The in vitro tests included survival in low pH and bile, antimicrobial activity (against Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica, Listeria monocytogenes, and Listeria innocua), resistance to 15 antibiotics and hemolytic tests. Among all 43 lactobacilli isolates, the 22 isolates showed a wide range of antimicrobial activity against 6 different pathogenic strains. There were twenty isolates growing at optimal temperature ranging 16~20 °C, which were initially considered to be cold-adapted strains. Two (2) Lb. sakei strains and 2 Lb. plantarum strains demonstrated the highest survivability after 4 h of exposure at pH 2.0. Most of the tested strains cannot be cultured after exposed into 0.5% bile solution for 4 h, while 2 Lb. plantarum strains (E-HLM-3, CQ-CGC-2) and 1 Lb. sakei strain M-DGM-2 survived even at 2% bile concentration. In addition, the safety assessment showed that 22 strains without any detectable hemolytic activity and resistant to glycopeptides (vancomycin, teicoplanin), levofloxacin, aztreonam, amikacin and oxacillin, while all the studied lactobacilli showed sensitivity to or semi-tolerant to other antibiotics. Conclusions Based on all the experiments, 3 strains, including E-HLM-3, CQ-CGC-2, and M-DGM-2 might be a candidate of choice for using in the food preservative agents and/or probiotic additives in feeding of aquaculture.

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Gardnerella vaginalis and Neisseria gonorrhoeae Are Effectively Inhibited by Lactobacilli with Probiotic Properties Isolated from Brazilian Cupuaçu (Theobroma grandiflorum) Fruit

BioMed Research International ◽

10.1155/2021/6626249 ◽

2021 ◽

Vol 2021 ◽

pp. 1-15

Author(s):

Nathan das Neves Selis ◽

Hellen Braga Martins de Oliveira ◽

Yan Bento dos Anjos ◽

Hiago Ferreira Leão ◽

Beatriz Almeida Sampaio ◽

...

Keyword(s):

Neisseria Gonorrhoeae ◽

Organic Acid ◽

Gardnerella Vaginalis ◽

In Vitro Tests ◽

Vaginal Mucosa ◽

Probiotic Properties ◽

Organic Acid Production ◽

Promising Source

In recent years, certain Lactobacillus sp. have emerged in health care as an alternative therapy for various diseases. Based on this, this study is aimed at evaluating in vitro the potential probiotics of five lactobacilli strains isolated from pulp of cupuaçu fruit fermentation against Gardnerella vaginalis and Neisseria gonorrhoeae. Our lactobacilli strains were classified as safe for use in humans, and they were tolerant to heat and pH. Our strains were biofilm producers, while hydrophobicity and autoaggregation varied from 13% to 86% and 13% to 25%, respectively. The coaggregation of lactobacilli used in this study with G. vaginalis and N. gonorrhoeae ranged from 15% to 36% and 32% to 52%, respectively. Antimicrobial activity was present in all tested Lactobacillus strains against both pathogens, and the growth of pathogens in coculture was reduced by the presence of our lactobacilli. Also, all tested lactobacilli reduced the pH of the culture, even in incubation with pathogens after 24 hours. The cell-free culture supernatants (CFCS) of all five lactobacilli demonstrated activity against the two pathogens with a halo presence and CFCS characterization assay together with gas chromatography revealed that lactic acid was the most abundant organic acid in the samples (50% to 62%). Our results demonstrated that the organic acid production profile is strain-specific. This study revealed that cupuaçu is a promising source of microorganisms with probiotic properties against genital pathogens. We demonstrated by in vitro tests that our Lactobacillus strains have probiotic properties. However, the absence of in vivo tests is a limitation of our work due to the need to evaluate the interaction of our lactobacilli with pathogens in the vaginal mucosa. We believe that these findings may be useful in developing a product containing our lactobacilli and their supernatants in order to support with vaginal health.

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Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity

Journal of Fungi ◽

10.3390/jof7100794 ◽

2021 ◽

Vol 7 (10) ◽

pp. 794

Author(s):

Camilla I. dos Santos ◽

Carmem D. L. Campos ◽

Wallace R. Nunes-Neto ◽

Monique S. do Carmo ◽

Flávio A. B. Nogueira ◽

...

Keyword(s):

Antimicrobial Activity ◽

Candida Species ◽

Genomic Analysis ◽

Probiotic Strain ◽

Gene Clusters ◽

In Vitro Assays ◽

Probiotic Properties ◽

Protein Coding ◽

Probiotic Potential

Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of L. fermentum. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from Enterococcus faecalis. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of Candida species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of L. fermentum ATCC 23271, which may provide insight into its future use given the functional benefits, especially against Candida infections.

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