scholarly journals KUALITAS SEMEN CAIR SAPI SIMENTAL MENGGUNAKAN LARUTAN ISOTONIS KOMERSIAL PADA KONSENTRASI DAN LAMA PENYIMPANAN BERBEDA

2017 ◽  
Vol 14 (2) ◽  
pp. 70
Author(s):  
Bayu Anke Mardian ◽  
Zumarni Zumarni ◽  
Anwar Effendi Harahap

Artificial insemination (AI) is a technique to insert the sperm or semen of bull thawed and processed first into the genital tract of female methods and a special tool called insemination gun, efforts to optimize the management of the cement in order to obtain the quality of cement is optimal to do with the selection of diluent cement. One alternative materials cement diluent is an isotonic solution Commercial (LIK). This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa cow simental. This research was conducted at the Regional Technical Implementation Unit of Artificial Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This study used a randomized block design (RAK) factorial design to Factor A (Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk + (40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day, days, B4 = 3 days. Each treatment consists of three replicates. Parameters measured were motility, percentage of survival, abrnormalitas, and MPU. These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours).

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety


2020 ◽  
Vol 25 (2) ◽  
pp. 68
Author(s):  
Nurul Afzan Hilda Zakiya ◽  
A H Yanti ◽  
T R Setyawati

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.


2020 ◽  
Vol 21 (2) ◽  
pp. 64
Author(s):  
Iman Sukirman ◽  
Eros Sukmawati ◽  
Siti Darojah Rasad ◽  
Nurcholidah Solihati

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.


2020 ◽  
Vol 12 (9) ◽  
pp. 173
Author(s):  
Tâmara Rebecca A. de Oliveira ◽  
Moysés Nascimento ◽  
Paulo R. Santos ◽  
Kleyton Danilo S. Costa ◽  
Thalyson V. Lima ◽  
...  

Changes in the relative performance of genotypes have made it necessary for more in-depth investigations to be carried out through reliable analyses of adaptability and stability. The present study was conducted to compare the efficiency of different informative priors in the Bayesian method of Eberhart & Russel with frequentist methods. Fifteen black-bean genotypes from the municipalities of Belém do São Francisco and Petrolina (PE, Brazil) were evaluated in 2011 and 2012 in a randomized-block design with three replicates. Eberhart & Russel’s methodology was applied using the GENES software and the Bayesian procedure using the R software through the MCMCregress function of the MCMCpack package. The quality of Bayesian analysis differed according to the a priori information entered in the model. The Bayesian approach using frequentist analysis had greater accuracy in the estimate of adaptability and stability, where model 1 which uses the a priori information, was the most suitable to obtain reliable estimates according to the BayesFactor function. The inference, using information from previous studies, showed to be imprecise and equivalent to the linear-model methodology. In addition, it was realized that the input of a priori information is important because it increases the quality of the adjustment of the model.


2018 ◽  
Vol 28 (2) ◽  
pp. 112
Author(s):  
Achmad Fadhli Aziz ◽  
Muhammad Ade Salim ◽  
Nurul Isnaini ◽  
Aulia Puspita Anugra Yekti ◽  
Trinil Susilawati

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (<em>Cocos rubescens</em>) and green (<em>Cocos viridis</em>) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-5<sup>0</sup>C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (<em>Tris</em>+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncan's multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 <span style="text-decoration: underline;">+</span> 1,1 %), viability (45,18<span style="text-decoration: underline;">+ </span>1,56 %), and abnormality (1,89<span style="text-decoration: underline;">+</span> 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 <span style="text-decoration: underline;">+</span> 1,3%) and (51<span style="text-decoration: underline;">+</span> 7,5%); Viability (67,14<span style="text-decoration: underline;">+1</span>7,99%) and (55,37<span style="text-decoration: underline;">+</span>10,66%), abnormality (1,23 <span style="text-decoration: underline;">+ </span>1,02%) dan (1,70<span style="text-decoration: underline;">+</span> 0,99%).


2020 ◽  
Vol 12 (1) ◽  
pp. 36-39
Author(s):  
Anis Dliyaul Haq ◽  
Irfan Irfan ◽  
Yanti Meldasari Lubis

Slaughtering is a method used to obtain food from farmed animals. Chicken slaughter is one of important factors affevting the halal and nutritional quality of meat. Chicken meat slaughtered is done according to islamic sharia and not according to Islamic sharia. Slaughtering is carried out according to islamic or halal sharia according LPPOM-MUI (2008). The storage time for food is a period of time that is safe and suitable for consumption in food and can be used by consumers according to their needs and can maintain the state of the food that is packed. Determine the quality of chicken meat whether or not accepted by consumers affect the physical, chemical and biological quality. The study aims to determine the effect of slaughtering methoda (according to Islamic sharia and not according to Islamic sharia) and duration of storage on the quality of chicken meat. This research uses factorial randomized block design (RBD) consisting of 2 factors. The first factor is the method of slaughtering, namely slaughtering by Islamic sharia and slaughtering not by Islamic sharia (neck slaughter broken). Slaughtering by Islamic sharia is chicken slaughtering in RPA that is halal certified, while slaughtering is not by Islamic sharia is chicken slaughtered in RPA that is certified not halal. The second factor is storage time, which is 0 hours (control), 3 hours, 6 hours and 9 hours. The result of this study showed that slaughter had a very significantly affected (P 0,01) on skin color. The treatmment duration significantly affected (P 0,05) on water content and suppleness. Research shows that the quality of chicken meat slaughtered according to Islamic sharia (P1) is better than meat slaughtered not according to Islamic sharia, especially in terms of; as higher skin color. As storage time increases, the water content


Author(s):  
M. Sai Kumar ◽  
M. Sarvana Perumal ◽  
G. Murugan ◽  
M. Vikram Sai ◽  
B. Maheswara Reddy

Background: Panchagavya is an organic formulation made from cow goods. The usage of fermented organic formulation with supportive beneficial microorganisms as foliar nourishment has come into the picture of modern agriculture for giving raise to good quality of non-residue protected food. Methods: A field experiment was conducted from June to October 2020 at Farmer’s field to study the Influence of Inorganic nutrients on Panchagavya on growth attributes of direct seeded Rice (BPT 5204). The experiment was laid out in a Factorial randomized block design in which factor A consists of Inorganic nutrients and Factor B consists of Panchagavya at different doses and time of applications. Factor A consists of viz., A1 100% RDF, A2 75% RDF and A3 50% RDF and Factor B consists of B1 No spraying, B2 Panchagavya 3% Spraying at 15, 30 and 45 DAS, B3 Panchagavya 3% Spraying at 10, 20, 30 and 45 DAS, B4 Panchagavya 4% Spraying at 15, 30 and 45 DAS B5 Panchagavya 4% Spraying at 10, 20, 30 and 45 DAS. Result: The results of the study revealed that the application of 100% Inorganic nutrients along with the foliar application of Panchagavya @ 4% at 4 times spraying showed the highest plant height, Number of effective tillers and leaf area index.


Jurnal Agro ◽  
10.15575/1205 ◽  
2017 ◽  
Vol 4 (1) ◽  
pp. 24-31
Author(s):  
Tarra Martiana Dewi ◽  
Anne Nurbaity ◽  
Pujawati Suryatmana ◽  
Emma Trinurani Sofyan

Quality of biofertilizer such as arbuscular myorrhizal fungi could be improved through the selection of appropriate sterilization and composition of the medium. The objective of the experiment was to determine the effect of sterilization and carrier composition on root colonisation, root length and weight of sorghum root. A green house experiment was conducted in randomized block design. The factors were: 1. methods of sterilization (ie. control; autoclave; NaOCl 10%; Co-60 gamma irradiation), and 2. composition of the carrier of mycorrhiza (100% zeolite; 75% zeolite + 25% rice charcoal;  50% zeolite + 50% rice charcoal). Results showed that there was no interaction effect of sterilization and composition of carrier on root colonisation, root length and weight of sorghum root. However, different technique of sterilisation gave differences on root colonisation, root length and weight of sorghum root. Sterilization using NaOCl 10% was not recommended. Gamma Co-60 irradiation sterilization did not have difference with control and autoclave, however there was  tendency  that irradiation technique gave better results to all parameters measured.


REVISTA FIMCA ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 28-31
Author(s):  
Darlan Darlan Sanches Barbosa Alves ◽  
Victor Mouzinho Spinelli ◽  
Marcos Santana Moraes ◽  
Carolina Augusto De Souza ◽  
Rodrigo da Silva Ribeiro ◽  
...  

Introdução: O estado de Rondônia se destaca como tradicional produtor de café, sendo o segundo maior produtor brasileiro de C. canephora. No melhoramento genético de C. canephora, a seleção de plantas de elevada peneira média está associada à bebida de qualidade superior. Objetivos: O objetivo desse estudo foi avaliar a variabilidade genética de clones de C. canephora para o tamanho dos grãos, mensurado a partir da avaliação da peneira média (PM). Materiais e Métodos: Para isso, foi conduzido ao longo de dois anos agrícolas experimento no campo experimental da Embrapa no município de Ouro Preto do Oeste-RO, para a avaliação da peneira média de 130 genótipos (clones) com características das variedades botânicas Conilon, Robusta e híbridos intervarietais. O delineamento experimental utilizado foi de blocos ao acaso, com quatro repetições de quatro plantas por parcela. Resultados: Não houve resultados significativos para a interação clones X anos, indicando uma maior consistência no comportamento das plantas ao longo do tempo. Porém foram observadas diferenças significativas para o tamanho dos grãos entre os genótipos avaliados, possibilitando selecionar genótipos superiores. Conclusão: Os genótipos agruparam-se em cinco classes de acordo com o teste de média, subsidiando a caracterização de um gradiente de variabilidade da característica avaliada ABSTRACTIntroduction: Coffea canephora accounts for approximately 35% of the world's coffee production. The state of Rondônia stands out as a traditional coffee producer, being the second largest Brazilian producer of C. canephora. In the classical genetic improvement of C. anephora, the selection of plants of high average sieve is associated with a drink of superior quality. Objectives: The objective of this udy was to evaluate the genetic variability of Coffea canephora clones for the agronomic medium sieve (PM). Materials and Methods: The experiment was conducted in the experimental field of Embrapa, municipality of OuroPreto do Oeste-RO, located at coordinates 10º44'53 "S and 62º12'57". One hundred thirty genotypes (clones) of botanical characteristics Conilon, Robusta and intervarietal hybrids were evaluated in the agricultural years 2013-2014 and 2014-2015. The experimental design was a randomized block design with four blocks and four plants per plot, spacing 3.5 x 1.5 meters between plants. Results: Significant difference was found for the grain size. According to the F test, at 5% probability, the genotypes were grouped into five classes according to the mean test. Conclusion: The results obtained subsidized the characterization of a variability gradient of the evaluated trait.


2020 ◽  
Vol 7 (04) ◽  
Author(s):  
A B Priyanshu ◽  
M K Singh ◽  
Mukesh Kumar ◽  
Vipin Kumar ◽  
Sunil Malik ◽  
...  

An experiment was conducted at Horticultural Research Centre, SVP University of Agriculture and Technology, Meerut (UP) during Rabi season of 2018-19 to assess the impact of different INM doses on yield and quality parameters of garlic. A total of ten treatments consisting of combinations of inorganic fertilizers, organic fertilizers and bio-fertilizers like T1- (Control), T2RDF (100:50:50 kg NPK ha-1), T3-RDF + 20 kg sulphur + FYM 20 ton ha-1, T4- RDF + 20 kg sulphur + VC 4 ton ha-1, T5- 75% RDF + 40 kg sulphur + 5 ton FYM ha-1+ PSB 5 kg ha-1, T6-75% RDF + 40 kg sulphur + 2 ton VC + Azotobacter 5 kg ha-1, T7- 75% RDF + 40 kg sulphur + FYM 3 ton + VC 1 ton+ PSB 5 kg + Azotobacter 5kg ha-1, T8- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg ha-1, T9- 50% RDF + 40 kg sulphur + FYM5 ton + VC 2 ton+ Azotobacter 5 kg ha-1and T10- 50% RDF + 40 kg sulphur + FYM 5 ton + VC2 ton + PSB 5 kg + Azotobacter 5 kg ha-1 were used in Randomized Block Design and replicated thrice. Out of these an application of T7 (75% RDF + 40 kg sulphur + FYM3 ton + VC 1 ton ha-1 + PSB 5 kg + Azotobacter 5 kg ha-1) was found to be significantly superior in term of yield and attributing parameters of garlic.


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