scholarly journals Viability of Peranakan Etawah Liquid Semen Preserved in Tris Substituted with Various Energy Sources

2020 ◽  
Vol 25 (2) ◽  
pp. 68
Author(s):  
Nurul Afzan Hilda Zakiya ◽  
A H Yanti ◽  
T R Setyawati
Keyword(s):  
Energy Source ◽  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Energy Sources ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Semen Preservation ◽  
Semen Extender ◽  
Fresh Semen

The use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days.

scholarly journals Pengaruh pengencer air kelapa tua yang berbeda varietas terhadap kualitas semen cair Kambing Boer pada penyimpanan 3-50C

2018 ◽  
Vol 28 (2) ◽  
pp. 112
Author(s):  
Achmad Fadhli Aziz ◽  
Muhammad Ade Salim ◽  
Nurul Isnaini ◽  
Aulia Puspita Anugra Yekti ◽  
Trinil Susilawati
Keyword(s):  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Materials Used ◽  
Red Variety ◽  
Fresh Semen ◽  
Range Test

Artificial Insemination using liquid semen is technology reproduction can improve goat productivity without liquid nitrogen. The Purpose of this research was to determine differences of old coconut water varieties of red (<em>Cocos rubescens</em>) and green (<em>Cocos viridis</em>) as diluent Boer goat semen, based on motility, viability and spermabnormalities during storage at 3-5<sup>0</sup>C. The materials used for this research was Boer goat fresh semen as much as 3 heads were collected 2 times a week. Experimental Design. The treatment were devided into three Treatmen, there were: P0 (<em>Tris</em>+ 20% egg yolk); P1 (old coconut water varieties red + 20% egg yolk); P2 (old coconut water varieties green + 20% egg yolk). The data were analyzed by randomized block design, if the result are different or significantly different then continued to be tested with Duncan's multiple range test. The result showed that during storage until days 4, P0 has the best result on motility (40,5 <span style="text-decoration: underline;">+</span> 1,1 %), viability (45,18<span style="text-decoration: underline;">+ </span>1,56 %), and abnormality (1,89<span style="text-decoration: underline;">+</span> 0,59 %). While on the diluent using old coconut water green variety better then red variety. On day of 2 P1 and P2 have motility (61 <span style="text-decoration: underline;">+</span> 1,3%) and (51<span style="text-decoration: underline;">+</span> 7,5%); Viability (67,14<span style="text-decoration: underline;">+1</span>7,99%) and (55,37<span style="text-decoration: underline;">+</span>10,66%), abnormality (1,23 <span style="text-decoration: underline;">+ </span>1,02%) dan (1,70<span style="text-decoration: underline;">+</span> 0,99%).

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals The Influence of Breed and Type of Extender on the Quality of Bull Semen

2020 ◽  
Vol 21 (2) ◽  
pp. 64
Author(s):  
Iman Sukirman ◽  
Eros Sukmawati ◽  
Siti Darojah Rasad ◽  
Nurcholidah Solihati
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Block Design ◽  
Egg Yolk ◽  
Spermatozoa Motility ◽  
Bull Semen ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Two Factors

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

269 THE USE OF A NEW EXTENDER FOR STORING FRESH BOVINE SEMEN FOR LONG PERIODS

2010 ◽  
Vol 22 (1) ◽  
pp. 291
Author(s):  
L. G. Frers ◽  
J. Hepburn ◽  
K. Hogan ◽  
C. Parminter ◽  
L. Mc Gowan ◽  
...  
Keyword(s):  
Ambient Temperature ◽  
Storage Period ◽  
Egg Yolk ◽  
Bovine Semen ◽  
Bull Semen ◽  
Frozen Semen ◽  
Long Life ◽  
Semen Extender ◽  
Fresh Semen

Processing bovine semen in fresh long life extender for use over 3 to 4 days after collection is a widely used technique in New Zealand (Shannon and Vishwanath 1995 Anim. Reprod. Sci. 39, 1-10). Advantages include greater use of valuable sires, transport without liquid nitrogen, and the possibility of more efficient use of sexed sorted semen. The new extender (Ext. A) also has the advantage of containing no egg yolk. This study compares this new long-life extender (Ext. A) with an existing product (Ext. B) and frozen/thawed semen. Semen from 12 different bulls was diluted to a concentration of 8 × 106 mL-1 and gradually cooled to 16°C. All samples were held at ambient temperature in the dark and motility was evaluated over a storage period of 4 days comparing the extenders. In this part of the trial Ext. A maintained motility better than Ext. B (P = 0.001) during the 4-day storage period (24 h: 90 v. 70%; 96 h: 85 v. 50%). The second part of the trial compared the conception rates (CR) in cows from the use of fresh long-term-extended semen and frozen/thawed semen. On 19 farms, 8546 cows were inseminated with fresh semen stored for 1 to 3 days and 7280 cows were inseminated with frozen semen. The overall CR at 7 to 8 weeks for the 19 farms was 73.7%. On 18 farms within the same farming group, 8498 cows received frozen semen and the CR was 71.1%. Pregnancy results were 2.6% (P = 0.001) higher CR at scanning in herds where fresh semen was used compared with the farms where only frozen/thawed semen was used (73.7 v. 71.1%). In the third part of the trial, semen from 4 different bulls were extended to 1 × 106 mL-1 in Ext. A and held at ambient temperature for 6 days prior to use for IVF. Our lab standard frozen/thawed bull semen was used as a control. Table 1 shows that semen held at ambient temperature in Ext. A for 6 days produced a similar percentage of transferable quality embryos to our IVP control frozen/thawed semen (26.9 v. 25.7%). We conclude that preserved bovine semen in fresh long-life extender for several days offers some advantages in AI and IVP programs compared with frozen semen. Table 1.Fresh semen extender (Ext.A) compared with frozen semen in IVP We appreciate the assistance of Liberty Genetics Ltd.

scholarly journals Total Bacteria of Post-Thawing Boer Buck Semen with Addition of Sweet Orange Essential Oil to Tris Yolk Extender

2020 ◽  
pp. 1-5
Author(s):  
Sukma Aditya Sitepu ◽  
Zaituni Udin ◽  
. Jaswandi ◽  
. Hendri
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Block Design ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Semen Storage ◽  
Research Procedure ◽  
Semen Extender ◽  
Total Bacteria ◽  
Orange Essential Oil

Aims: To determine the effect of the addition of sweet orange essential oil to the extender (tris yolk extender without the addition of antibiotics/antibacterial) to total bacteria Boer Buck frozen semen. Study Design: Randomized Block Design. Place and Duration of Study: Sample: Laboratory Reproduction of Loka Penelitian Kambing Potong Sei Putih Indonesia, between January and March 2019. Methodology: The research procedure starts with the preparation of semen extender, collection of fresh semen, dilution of semen, equilibration, freezing of semen, and thawing. This research obtained conducted using a Randomized Block Design consisting of 5 treatment levels and five replications. Semen storage using 3 Boer buck, which done for three days. As a treatment is the addition of sweet orange essential oil as much as (P0) 0%, (P1) 0,25%, (P2) 0,5%, (P3) 0,75% and (P4) 1% on the tris yolk extender. The observed variables was total bacteria evaluated before freezing and after freezing (Post-Thawing). Results: The results showed that the addition of sweet orange essential oil had a very significant effect (P <0.01). The results of adding sweet orange essential oil to the extender (Post-Thawing) were 78.6 x 102 CFU (P0), 76 x 102 CFU (P1), 73,2 x 102 CFU (P2), 71 x 102 CFU (P3) and 68.6 x 102 CFU (P4). Conclusion: The best values the total bacteria 68.6 x 102 CFU (P4). It can conclude that the best result of adding sweet orange essential oil to tris yolk extender is (P4) 1%.

scholarly journals KUALITAS SEMEN CAIR SAPI SIMENTAL MENGGUNAKAN LARUTAN ISOTONIS KOMERSIAL PADA KONSENTRASI DAN LAMA PENYIMPANAN BERBEDA

2017 ◽  
Vol 14 (2) ◽  
pp. 70
Author(s):  
Bayu Anke Mardian ◽  
Zumarni Zumarni ◽  
Anwar Effendi Harahap
Keyword(s):  
Artificial Insemination ◽  
Block Design ◽  
Egg Yolk ◽  
Isotonic Solution ◽  
Factor B ◽  
Randomized Block Design ◽  
Duration Of Storage ◽  
Alternative Materials ◽  
Selection Of

Artificial insemination (AI) is a technique to insert the sperm or semen of bull thawed and processed first into the genital tract of female methods and a special tool called insemination gun, efforts to optimize the management of the cement in order to obtain the quality of cement is optimal to do with the selection of diluent cement. One alternative materials cement diluent is an isotonic solution Commercial (LIK). This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa cow simental. This research was conducted at the Regional Technical Implementation Unit of Artificial Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This study used a randomized block design (RAK) factorial design to Factor A (Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk + (40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day, days, B4 = 3 days. Each treatment consists of three replicates. Parameters measured were motility, percentage of survival, abrnormalitas, and MPU. These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours).

scholarly journals Repeatability and Phenotypic Correlation Among Semen Quality Traits in Holstein Bulls

2018 ◽  
Vol 42 (4) ◽  
Author(s):  
Argi Argiris ◽  
Siswanto Imam Santoso ◽  
Yon Supri Ondho ◽  
Edy Kurnianto
Keyword(s):  
Artificial Insemination ◽  
Semen Quality ◽  
Intraclass Correlation ◽  
Correlation Method ◽  
Phenotypic Correlation ◽  
Quality Traits ◽  
Frozen Semen ◽  
Holstein Bulls ◽  
Fresh Semen ◽  
Selection Of

The purpose of this research was to analysis the value of repeatability and correlation among the traits affecting the production of frozen semen from Holstein’s bull in Indonesia. Repeatability and correlation were calculated based on the data of frozen semen production of 15.699 records from 44 Holstein bulls at Singosari Artificial Insemination Center (SAIC) and 8.935 records from 39 Holstein bulls at Lembang Artificial Insemination Center (LAIC). Repeatability for volume, motility, fresh semen concentration and frozen semen production was evaluated by intraclass correlation method. The repeatability values of LAIC for volume, motility, fresh semen concentration and frozen semen production were 0.60; 0.54; 0.37 and 0.47. The repeatability values of SAIC for volume, motility, fresh semen concentration and frozen semen production were 0.54; 0.30; 0.43 and 0.29. The linear correlation value between volume, motility and fresh semen concentration with the amount of semen produced per collections were 0.41, 0.36, and 0.58. Concentration was the most factors influencing the number of frozen semen produced. The effectiveness of the selection of Holstein's frozen semen producing could be determined by the value of repeatability and the phenotypic correlation among semen quality traits such as volume, motility, concentration and frozen semen production.

A COMPARISON OF FRESH AND FROZEN SEMEN IN THE INSEMINATION OF CONFINED SHEEP

1979 ◽  
Vol 59 (4) ◽  
pp. 685-691 ◽  
Author(s):  
G. A. LANGFORD ◽  
G. J. MARCUS ◽  
A. J. HACKETT ◽  
L. AINSWORTH ◽  
H. F. PETERS ◽  
...  
Keyword(s):  
Litter Size ◽  
Reproductive Performance ◽  
Artificial Insemination ◽  
Embryonic Mortality ◽  
Frozen Semen ◽  
Serum Gonadotropin ◽  
Early Embryonic Mortality ◽  
The Difference ◽  
Natural Service ◽  
Fresh Semen

The reproductive performance of crossbred sheep maintained in total confinement was compared after artificial insemination with fresh or frozen semen. Estrus was synchronized with progestagen-impregnated vaginal sponges and pregnant mares’ serum gonadotropin. Inseminations were performed 54 and 60 h after sponge removal. The fertility of ewes inseminated with fresh semen was significantly higher than of ewes inseminated with frozen semen. Conception rates, lambing rates and litter size were 83%, 78% and 2.2 using fresh semen and 65%, 43% and 1.8 using frozen semen. In a group of similar ewes bred by natural service, the lambing performance was comparable to that obtained with fresh semen. The difference between conception and lambing rates suggests an increase in early embryonic mortality when breeding with frozen semen and confirms the need for improved frozen semen technology.

scholarly journals Effect of Gum Arabic on Stallion Sperm Survival During Cold Storage and Post Freezing

2018 ◽  
Vol 41 (1) ◽  
pp. 21-31
Author(s):  
Mohamed Ali ◽  
Musa M. Musa ◽  
Sulaiman Alfadul ◽  
K. Al-Sobayel
Keyword(s):  
Gum Arabic ◽  
Egg Yolk ◽  
High Viscosity ◽  
Motile Sperm ◽  
Sperm Velocity ◽  
Low Viscosity ◽  
Straight Line ◽  
Frozen Semen ◽  
Sperm Survival ◽  
Semen Extender

Abstract This study is aimed at investigating effects of supplementation of stallion’ semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 μm/sec) and immotile (0-45 μm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.

scholarly journals Pemanfaatan Ampas Sagu sebagai Mulsa Organik dalam Peningkatkan Pertumbuhan Tanaman Sagu (Metroxylon sagu Rottb.) Di Lapang

Cassowary ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 112-118
Author(s):  
Mikhael Sada ◽  
Florentina Heningtyas Listyorini ◽  
Barahima Abbas
Keyword(s):  
Experimental Method ◽  
Plant Height ◽  
Leaf Growth ◽  
Block Design ◽  
Height Growth ◽  
Energy Sources ◽  
Sago Palm ◽  
Randomized Block Design ◽  
The World ◽  
Metroxylon Sagu

Sago palm need to be cultivated intensively for increasing carbohydrate resources to anticipate the deficiency of energy sources of the world. This study aims to measure the influences of sago dregs to the sag palm growth in the field. The experimental method used in this works was Randomized Block Design that consisting of five treatments sago dregs weights in the russet stages of sago palm. The composition of the treatment is follows: without sago dregs (P0), 5 kg sago dregs tree-1 (P1), 10 kg of sago dregs tree-1 (P2), 15 kg sago dregs tree-1 (P3), 20 kg sago dregs tree-1 (P4). The results showed that sago dregs had a significant effect of the spear leaves growth, but not significantly effect of the plant height growth, number of leaflets growth, and number of leaflets. Utilization of 10 kg sago dregs tree-1 as mulch was calculated influence of the spear leaf growth and utilization of 20 kg sago dregs as mulch inhibitory of sago palm growth.

Sign in / Sign up

Export Citation Format

Share Document