Temporal dynamics of change of polycrystalline component of bioctisses and blood of death from poisoning by alcohol and carbon with different time since death

Forensic-medical examination ◽

10.24061/2707-8728.2.2021.8 ◽

2021 ◽

pp. 63-73

Author(s):

Ihor Ivaskevych ◽

Oleh Vanchulyak ◽

Yurii Ushenko ◽

Artem Motrich

Keyword(s):

Adrenal Glands ◽

High Efficiency ◽

Temporal Dynamics ◽

Carbon Monoxide Poisoning ◽

Analytical Determination ◽

Linear Birefringence ◽

Internal Organs ◽

Linear Change ◽

Polarization Phase

The article contains the results of a study of the effectiveness of forensic medical determination of the prescription of death by the method of differential Müller-matrix polarization-phase tomography, which is implemented by multichannel probing of rhizopolarization with laser beams of histological sections of the brain, myocardium, adrenal glands, liver and blood of the deceased and multichannel polarization filtration series images with algorithmic reproduction (reconstruction) of coordinate distributions (tomograms) of the average linear birefringence (LD) of polycrystalline networks. Aim of the work. Efficiency study by the method of differential Müller-matrix polarization-phase tomography for determining the duration of death. Materials and methods. The object of the study was the histology of samples of human internal organs (brain, myocardium, adrenal glands, and liver) with different duration of damage from 6 hours to 48 hours (we used BT samples from those who died from alcohol poisoning and carbon monoxide poisoning). For control, we used BT samples from those who died from ischemic heart disease with different duration of damage from 6 hours to 48 hours. The studies were carried out using the method of differential Muller-matrix polarization-phase tomography. Results. The range of linear change in the magnitude of the higher-order statistical moments, and accordingly the analytical determination of the DNS, lies in the range from 20 hours to 48 hours. The accuracy of determining the DNS is also significantly improved and amounts to 0.5 hours. Conclusion. The high efficiency of Muller matrix algorithmic reproduction of maps of average values of birefringence of the optically anisotropic component of biological preparations of the deceased was revealed.

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OPTIMIZATION OF PESTICIDES RESIDUES ANALYTICAL CONTROL IN CEREAL CROPS PROTECTION SYSTEM

Medical Science of Ukraine (MSU) ◽

10.32345/2664-4738.3-4.2019.12 ◽

2019 ◽

Vol 15 (3-4) ◽

pp. 76-84

Author(s):

M.M. Korshun ◽

O.M. Korshun ◽

A.O. Lipavska ◽

T.I. Zinchenko ◽

S.V. Bilous ◽

...

Keyword(s):

Quantitative Determination ◽

High Efficiency ◽

Negative Impact ◽

Protection System ◽

Cereal Crops ◽

Analytical Determination ◽

Analytical Control ◽

Simultaneous Presence ◽

Air Samples

Relevance. The scientific substantiation of the method selection, the development of conditions for qualitative identification and quantitative determination of Axial 050 EC, Peak 75 WG, Tilt 250 EC, Magnello 350 EC, Enzhio 247 SS, Karate Zeon 050 CC pesticides (by its simultaneous presence) used in cereal crops protection system was given in the article, as well as preparation conditions for the analysis of water and air samples were described, which will allow to control the established hygienic standards and minimize pesticide negative impact on population health and the environment. Objective of the research is to develop methods for the analytical determination of 9 active substances of 7 pesticide preparations applied in the wheat and barley protection system with its simultaneous presence in water and air. Materials and methods. Chromatographic analysis was performed by Shimazu (Japan) liquid chromatograph. Chromatograph control, recording, analysis and storage of chromatographic data were performed using S/w LC solution software. The package of IBM SPSS StatisticsBase v.22 and MS Exel statistical programs was used for statistical processing of results. Results. The developed optimal conditions of sample preparation and chromatographic determination in the combined presence of 9 different chemical classes pesticides used in the cereal crops protection system will allow to control their content with the limit of quantitative determination of each compound 0,001 mg/dm3 in the water sample, and 0,05 mg/m3 in the air, thus allowing control the established hygienic standards of these compounds in the water and in the working zone air. Conclusions. The conditions for simultaneous determination of prosulfuron and pinoxaden herbicides, thiamethoxam and lambda-cyhalothrin insecticides, fludioxonil, sedaxane, tebuconazole, propiconazole, diphenoconazole fungicides in its combined presence in the water or air samples by high-efficiency liquid chromatography method were elaborated allowing the efficiency of analysis to be increased and its total price to be reduced.

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