scholarly journals Chemical Constituents of the Insecticidal Active Extract of Tinospora crispa

2019 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Nor Aziyah Bakhari ◽  
Siti Nur Amirah Diana Fadzillah ◽  
Norain Isa
Keyword(s):  
Blood Pressure ◽  
Petroleum Ether ◽  
Silica Gel ◽  
1H Nmr ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
First Time

Tinospora crispa Miers (Menispermaceae) is a climbing vine with stems rich in warts. The plant is called Akar Seruntum or Patawali in Malaysia and is widely used for treating skin complaints, malaria, bacterial abscess, high blood pressure and diabetes. In the present study, the stems of T. crispa were collected from the locality and succesively extracted with petroleum ether, followed by chloroform and ethanol. The insecticidal active extract (ethanol extract) was  subjected to column chromatography of silica gel eluted with a gradient mobile phase containing hexane, chloroform and ethanol. Among the chemical constituents isolated are n-tetracosyl trans-ferulate and n-octacosyl alcohol, along with three known aporphine alkaloids; N-formylnornuciferine, N-acetylnornuciferine and lysicamine. All compounds were identified by comparing their spectroscopic data (UV, IR, 1H NMR, MS) with data from corresponding values in the literature. Isolation of n-tetracosyl trans-ferulate and n-octacosyl alcohol is reported the first time for T. crispa.

scholarly journals CHEMICAL CONSTITUENTS AND BIOACTIVITY OF Codium amplivesiculatum SETCHELL N. L.GARDNER (CHLOROPHYTA; BRYOPSIDALES)

2013 ◽  
Vol 28 (2) ◽  
pp. 1
Author(s):  
A. Marín -Álvarez ◽  
J. I. Murrillo -Álvarez ◽  
M. Muñoz -Ochoa ◽  
G. M. Molina -Salinas
Keyword(s):  
Staphylococcus Aureus ◽  
Silica Gel ◽  
Vibrio Parahaemolyticus ◽  
Marine Alga ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Solvent Mixtures ◽  
Bioactive Substances ◽  
First Time ◽  
Tuberculosis Activity

In search of bioactive substances from Mexican marine organisms, crude ethanol-extract from the marine alga Codium amplivesiculatum was fractionated in chromatographic columns of silica gel at 60 Å (230-400 mesh) using solvent mixtures of increasing polarity. All the fractions were submitted to antibacterial assays. The major metabolite from an anti-tuberculosis fraction (MIC = 100 μg mL–1) was purified and identified as 1-octodecanol (1). The anti-tuberculosis activity was attributed to 1 with bases in previous reports. In addition, clerosterol (2) was obtained by crystallization from an active fraction against Staphylococcus aureus and Vibrio parahaemolyticus (MIC = 125 and 250 μg mL–1, respectively). Both structures were established by interpretation and comparison of infrared and 1H NMR spectroscopic data. In contrast with other studies, 2 showed a non-significant cytotoxicity against the cell line PC-3 (% GI = 21.05 ± 0.3 at 50 μg mL–1). To our knowledge, these metabolites are reported for the first time from C. amplivesiculatum, and this is one of very rare reports of saturated long-chain alcohols isolated from chlorophytes. Constituyentes químicos y bioactividad de Codium amplivesiculatum Con el propósito de descubrir sustancias bioactivas a partir de organismos marinos encontrados en México, se fraccionó el extracto crudo etanólico de Codium amplivesiculatum en columnas cromatográficas de sílica gel 60 Å (230-400 de malla) utilizando mezclas de solventes de polaridad creciente. Todas las fracciones se sometieron a ensayos antibacterianos. El principal metabolito de la fracción activa antituberculosis (MIC = 100 μg mL-1), fue purificado e identificado como 1-octodecanol (1). La actividad antituberculosis, basada en reportes previos, se atribuyó al compuesto 1. Además, se obtuvo clerosterol (2) por cristalización de una fracción activa frente a Staphylococcus aureus y Vibrio parahaemolyticus (MIC = 125 y 250 μg mL-1, respectivamente). Las dos estructuras fueron inferidas mediante interpretación y comparación de datos obtenidos por espectroscopía de IR-ATR y 1H RMN. En contraste con otros estudios, el compuesto 2 mostró una citotoxicidad no significativa contra la línea celular PC-3 (% IC = 21.05 ± 0.3 a 50 μg mL–1). Hasta donde sabemos, estos metabolitos se reportan por primera vez en C. amplivesiculatum y 1-octadecanol es un reporte muy raro de alcohol de cadena larga aislado de clorofitas.

Chemical Constituents of the Lichen Cladina macaronesica

1991 ◽  
Vol 46 (1-2) ◽  
pp. 12-18 ◽  
Author(s):  
Antonio G . González ◽  
Jaime Bermejo Barrera ◽  
Elsa Ma Rodríguez Pérez ◽  
Consuelo E. Hernández Padrón
Keyword(s):  
Natural Products ◽  
Phenolic Compounds ◽  
Silver Nitrate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Chemical Constituents ◽  
Usnic Acid ◽  
Acetone Extract ◽  
First Time

Column chromatography of the acetone extract of the lichen Cladina macaronesica (Sephadex LH-20, silica gel and silver nitrate-im pregnated silica gel) afforded eight triterpenes identified by chemical and spectral m eans. α-Amyrenone, lupenone, taraxerol, taraxerone and /so-arborinol acetate were isolated for the first time from lichens and (-)-usnic acid and five mononuclear phenolic compounds were also obtained, four for the first time as natural products. The possible transformation of perlatolic acid into these phenolic compounds is briefly outlined.

scholarly journals UJI EFEKTIVITAS DAN IDENTIFIKASI SENYAWA AKTIF EKSTRAK DAUN SIRSAK SEBAGAI PESTISIDA NABATI TERHADAP MORTALITAS KUTU DAUN PERSIK (Myzus persicae Sulz) PADA TANAMAN CABAI MERAH (Capsipcum annum L.)

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Ni Made Dwi Desiyanti ◽  
I Made Dira Swantara ◽  
I Putu Sudiarta
Keyword(s):  
Ir Spectra ◽  
Silica Gel ◽  
Column Chromatography ◽  
Myzus Persicae ◽  
Mobile Phase ◽  
Ethanol Extract ◽  
Annona Muricata ◽  
Isolation And Identification ◽  
Butanol Extract ◽  
Metabolite Extraction

The study of isolation and identification of the active compounds of soursop (Annona muricata L.) leave extract were conducted . The metabolite extraction was conducted using maceration method with 96 % ethanol. The ethanol extract was used to test the mortility of aphid (Myzus persicae S.), with LC50 of 100 ppm. The n-hexane, chloroform, and n-buthanol were used to fractionate the ethanol extract. The mortality test of those three extracts showed the LC50 of 545.12 ppm, 136.26 ppm and 117.73 ppm, respectively. The n-butanol extract was separated using silica gel column chromatography with chloroform: ethanol: water (5:4:1), as the mobile phase. The fractions resulted were FI, FII, FIII, FIV and FV. The mortality test indicated that FII was the best with LC50 of 596.48 ppm. The FII was purified using silica gel column chromatography, resulting three fractions (FII.1, FII.2 and FII.3).  The mortality test of those fractions indicated that FII.2 showed the best result with LC50 of 601.17 ppm. The UV-Vis and IR spectra showed that FII.2 fraction contained flavonoides under the flavonon family.

Identification of Biflavones in Ethyl Acetate Fraction from Ethanol Extract of Selaginella doederleinii Hieron

2012 ◽  
Vol 550-553 ◽  
pp. 1862-1865
Author(s):  
Yan Zhang ◽  
Li Yang ◽  
Lin Wang
Keyword(s):  
Physicochemical Properties ◽  
Ethyl Acetate ◽  
Dimethyl Ether ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Chemical Structures ◽  
Trimethyl Ether ◽  
First Time

The chemical constituents were isolated from the ethyl acetate fraction from ethanol extract of Selaginella doederleinii Hieron by column chromatography on silica gel and Sephadex LH-20. The chemical structures of six biflavones were elucidated on the basis of physicochemical properties and spectroscopic data as amentoflavone (1), robustaflavone 7,4',7''-o-trimethyl ether (2), heveaflavone (3), podocarpusflavone A (4), robustaflavone 4',4'''-o-dimethyl ether (5) and robustaflavone 4'-o-methyl ether (6). Biflavones 2, 4 and 5 were obtained from Selaginella doederleinii Hieron for the first time.

Chemical Constituents from the Rhizomes of Cyperus Rotundus L.

2017 ◽  
Vol 10 (1) ◽  
pp. 82-91
Author(s):  
Shahnaz Sultana ◽  
Mohammed Ali ◽  
Showkat Rasool Mir
Keyword(s):  
Petroleum Ether ◽  
Silica Gel ◽  
Chemical Constituents ◽  
Cyperus Rotundus ◽  
Fatty Esters ◽  
Aliphatic Ketones ◽  
Phytochemical Investigation ◽  
Soxhlet Apparatus ◽  
Spectral Data Analysis ◽  
First Time

Background: Cyperus rotundus L. (Cyperaceae), is a perennial sedge distributed throughout India and other parts of the world. Its tubers are used as an appetizer, febrifuge and to treat bleeding, blisters, boils, cough, diarrhea, inflammation, lacteal disorders, rheumatoid arthritis, stomach ailments, skin rashes, thirst, vomiting, worm infestation and wounds. Objective: Our study was planned to isolate chemical constituents from the rhizomes of C. rotundus and to characterized their structures. Method: The air-dried rhizome powder was exhaustively extracted with methanol in a Soxhlet apparatus. The concentrated methanol extract was adsorbed on silica gel (60-120 mesh) for the preparation of a slurry. The dried slurry was chromatographed over silica gel column packed in petroleum ether. The column was eluted with petroleum ether, chloroform and methanol, successively, in order of increasing polarity to isolate the compounds. Results: Phytochemical investigation of the tubers led to isolate a sesquiterpenone characterized as 12-methyl cyprot-3-en-2-one-13-oic acid (1), two aliphatic ketone viz. n-dotriacontan-15-one (2) and n-tetracontan-7-one (8), fatty esters n-pentadecanyl octadec-9, 12- dienoate (n-pentadecanyl linoleate, 3), n-hexadecanyl linoleate (4), n-hexadecanyl oleate (5) and n-pentacos-13ʹ-enyl octadec-9-enoate (n-pentacos-13ʹ-enyl oleate, 9), two steroidal esters stigmast-5,22–dien-3β –olyl n-dodecanoate (stigmasterol laurate, 6) and stigmast-5, 22-dien-3β-olyl n-tetradecanoate (stigmasterol myristate, 7), β-sitosterol-3β-O-glucoside (10) and a triterpenic glycosidic ester lup-12, 20 (29)-dien-3β-ol-3-α-L-arabinopyranosyl-2'-oleate (lupenyl 3β-O-arabinpyranosyl 2′-oleate, 11). The structures of these compounds were established by spectral data analysis and chemical reactions. Conclusion: A sesquiterpene identified as cyprot-3-en-2-one-14-oic acid, two aliphatic ketones, fatty esters, two steroidal esters, β-sitosterol-3β-O-glucoside and lupenyl 3β-O-arabinpyranosyl 2′-oleate were isolated for the first time from the rhizomes.

Chemical Constituents from the Root and Stem of Zanthoxylum avicennae

2014 ◽  
Vol 618 ◽  
pp. 426-430 ◽  
Author(s):  
Tao Guo ◽  
Xiao Feng Tang ◽  
Ju Bao Zhang ◽  
Jun Qing Wei ◽  
Ya Wang ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Hydroxybenzoic Acid ◽  
Etoac Extract ◽  
Data Analyses ◽  
First Time

Objective: To study the chemical constituents from the root and stem of Zanthoxylum avicennae. Method: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. Result: Fourteen compounds were isolated from the EtOAc extract and identified as Avicennin (1), Xanthoxyletin (2), Luvangetin (3), Norchelerythrine (4), Diisobutyl phthalate (5), β-amyrin (6), Lupeol (7), Chelerythrine (8), Dictamine (9), γ-fagarine (10) and Skimmianine (11), Sesamin (12) Syringaresinol (13) and P-hydroxybenzoic acid (14). Conclusion: The compounds 2, 4, 5, 6, 7, 12 and 14 were obtained from this plant for the first time.

scholarly journals Structure Elucidation of an Pentacyclic Triterpenoid and Phenolic from Steam Bark of Vitex Pubescens Vahl

2019 ◽  
Vol 1 (1) ◽  
pp. 68-74
Author(s):  
Lenny Anwar
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Betulinic Acid ◽  
Spectroscopic Data ◽  
Structure Elucidation ◽  
Stem Bark ◽  
Hydroxybenzoic Acid ◽  
Pentacyclic Triterpenoid ◽  
Reported Data ◽  
First Time

Pentacyclic triterpenoid, betulinic acid (1) and phenolic, p-hydroxybenzoic acid (2), had been isolated for the first time from the stem bark of Vitex pubescens Vahl. The structure of compounds 1 and 2 was determined based on the interpretation of spectroscopic data including UV, IR, NMR (1H-NMR, 13C-NMR, HMQC, HMBC, COSY) and MS, as well as by comparison with those reported data.

scholarly journals Chemical Constituents and Derivatization of Melodorinol from the Roots of Melodorum fruticosum

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000
Author(s):  
Siriwat Hongnak ◽  
Jongkolnee Jongaramruong ◽  
Suttira Khumkratok ◽  
Pongpun Siriphong ◽  
Santi Tip-pyang
Keyword(s):  
Cell Lines ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Chemical Investigation ◽  
Crude Extracts ◽  
First Time ◽  
Mcf 7

Chemical investigation of the CH2Cl2 and MeOH crude extracts of the roots of Melodorum fruticosum Lour. led to the isolation of 15 known compounds, of which 5, 10, and 12-15 are reported for the first time from this plant. In addition, melodorinol (7) was derivatized to afford six new (7a-7d and 7f-7g) analogues and one known compound (7e). Their structures were identified on the basic of spectroscopic data. Most of them were evaluated for their cytotoxicity against KB, HeLa, MCF-7 and HepG-2 cell lines. Compounds 4 and 7b were the most potent to all cell lines.

scholarly journals ISOLATION AND PHYTOCHEMICAL TEST OF ANTICANCER ISOLATE OF SPONGE Hyrtios erecta

2017 ◽  
Vol 1 (1) ◽  
pp. 16
Author(s):  
I Made Dira Swantara ◽  
Wiwik Susanah Rita ◽  
Rr Anisa Hernindy
Keyword(s):  
Stationary Phase ◽  
Anticancer Activity ◽  
Silica Gel ◽  
Screening Test ◽  
Mobile Phase ◽  
Room Temperature ◽  
Ethanol Extract ◽  
Toxicity Screening ◽  
Polyphenol Compounds ◽  
Thousand Islands

AbstractThis research aims to isolate and phytochemically test of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from the waters of Pari Island beach, Thousand Islands (Jakarta). Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on Bhrine Shrimp Lethality Test (BSLT). The compounds of the active isolate were performed by phytochemical test. Based on the results, it was found that the toxic isolate of Hyrtios erecta sponges has anticancer activity with IC50 of 30,497 ppm. The anticancer isolate contained alkaloid, steroid, and polyphenol compounds

Chemical Constituents from Euphorbia helioscopia

2011 ◽  
Vol 396-398 ◽  
pp. 1337-1340 ◽  
Author(s):  
Di Geng ◽  
Lian Jin Weng ◽  
Yuan Yuan Han ◽  
Xin Yang
Keyword(s):  
Silica Gel ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Nmr Spectrum ◽  
Euphorbia Helioscopia ◽  
First Time ◽  
Spectrum Data

AIM: To study the chemical constituents of Euphorbia helioscopia. METHODS: Compounds 1-10 were isolated and purified by silica gel, Sephadex LH-20 and Rp-18 chromatogarphy. Their structures were elucidated mainly by spectroscopic methods. RESULTS: Ten known compounds, helioscopinolide A(1), helioscopinolide B(2), scopoletin(3), scoparone(4), isoscopoletin(5), licochalone A(6), quercelin(7), 7, 4’-dihydroxy-5-methoxy flacanone(8), 2’, 4’-dihydroxy-6’-methoxydihydrochalcone(9) and pinocembrin(10), were isolated and structurally elucidated. CONCLUSION: Compound 3-5 and 8-10 were isolated from this plant for the first time. 2D NMR spectrum data of 2 were also reported in this paper.

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