Chemical Constituents from the Root and Stem of Zanthoxylum avicennae

2014 ◽  
Vol 618 ◽  
pp. 426-430 ◽  
Author(s):  
Tao Guo ◽  
Xiao Feng Tang ◽  
Ju Bao Zhang ◽  
Jun Qing Wei ◽  
Ya Wang ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Hydroxybenzoic Acid ◽  
Etoac Extract ◽  
Data Analyses ◽  
First Time

Objective: To study the chemical constituents from the root and stem of Zanthoxylum avicennae. Method: The constituents were separated by column chromatography, and their structures were elucidated by spectroscopic data analyses. Result: Fourteen compounds were isolated from the EtOAc extract and identified as Avicennin (1), Xanthoxyletin (2), Luvangetin (3), Norchelerythrine (4), Diisobutyl phthalate (5), β-amyrin (6), Lupeol (7), Chelerythrine (8), Dictamine (9), γ-fagarine (10) and Skimmianine (11), Sesamin (12) Syringaresinol (13) and P-hydroxybenzoic acid (14). Conclusion: The compounds 2, 4, 5, 6, 7, 12 and 14 were obtained from this plant for the first time.

scholarly journals Chemical Constituents of the Insecticidal Active Extract of Tinospora crispa

2019 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Nor Aziyah Bakhari ◽  
Siti Nur Amirah Diana Fadzillah ◽  
Norain Isa
Keyword(s):  
Blood Pressure ◽  
Petroleum Ether ◽  
Silica Gel ◽  
1H Nmr ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
First Time

Tinospora crispa Miers (Menispermaceae) is a climbing vine with stems rich in warts. The plant is called Akar Seruntum or Patawali in Malaysia and is widely used for treating skin complaints, malaria, bacterial abscess, high blood pressure and diabetes. In the present study, the stems of T. crispa were collected from the locality and succesively extracted with petroleum ether, followed by chloroform and ethanol. The insecticidal active extract (ethanol extract) was  subjected to column chromatography of silica gel eluted with a gradient mobile phase containing hexane, chloroform and ethanol. Among the chemical constituents isolated are n-tetracosyl trans-ferulate and n-octacosyl alcohol, along with three known aporphine alkaloids; N-formylnornuciferine, N-acetylnornuciferine and lysicamine. All compounds were identified by comparing their spectroscopic data (UV, IR, 1H NMR, MS) with data from corresponding values in the literature. Isolation of n-tetracosyl trans-ferulate and n-octacosyl alcohol is reported the first time for T. crispa.

scholarly journals Structure Elucidation of an Pentacyclic Triterpenoid and Phenolic from Steam Bark of Vitex Pubescens Vahl

2019 ◽  
Vol 1 (1) ◽  
pp. 68-74
Author(s):  
Lenny Anwar
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Betulinic Acid ◽  
Spectroscopic Data ◽  
Structure Elucidation ◽  
Stem Bark ◽  
Hydroxybenzoic Acid ◽  
Pentacyclic Triterpenoid ◽  
Reported Data ◽  
First Time

Pentacyclic triterpenoid, betulinic acid (1) and phenolic, p-hydroxybenzoic acid (2), had been isolated for the first time from the stem bark of Vitex pubescens Vahl. The structure of compounds 1 and 2 was determined based on the interpretation of spectroscopic data including UV, IR, NMR (1H-NMR, 13C-NMR, HMQC, HMBC, COSY) and MS, as well as by comparison with those reported data.

scholarly journals Chemical Constituents and Derivatization of Melodorinol from the Roots of Melodorum fruticosum

2015 ◽  
Vol 10 (4) ◽  
pp. 1934578X1501000
Author(s):  
Siriwat Hongnak ◽  
Jongkolnee Jongaramruong ◽  
Suttira Khumkratok ◽  
Pongpun Siriphong ◽  
Santi Tip-pyang
Keyword(s):  
Cell Lines ◽  
Spectroscopic Data ◽  
Chemical Constituents ◽  
Chemical Investigation ◽  
Crude Extracts ◽  
First Time ◽  
Mcf 7

Chemical investigation of the CH2Cl2 and MeOH crude extracts of the roots of Melodorum fruticosum Lour. led to the isolation of 15 known compounds, of which 5, 10, and 12-15 are reported for the first time from this plant. In addition, melodorinol (7) was derivatized to afford six new (7a-7d and 7f-7g) analogues and one known compound (7e). Their structures were identified on the basic of spectroscopic data. Most of them were evaluated for their cytotoxicity against KB, HeLa, MCF-7 and HepG-2 cell lines. Compounds 4 and 7b were the most potent to all cell lines.

scholarly journals Isolation of Phenolics from Rhizophora mangle by Combined Counter-current Chromatography and Gel-Filtration

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Fernanda das Neves Costa ◽  
Marcos Daniel da Silva ◽  
Ricardo Moreira Borges ◽  
Gilda Guimarães Leitão
Keyword(s):  
Phenolic Compounds ◽  
Leaf Extract ◽  
Gel Filtration ◽  
Rhizophora Mangle ◽  
Solvent System ◽  
Hydroxybenzoic Acid ◽  
Etoac Extract ◽  
Counter Current ◽  
First Time ◽  
Tof Ms

Nine phenolic compounds, quercetin, epi-catechin, catechin, 4-hydroxybenzoic acid, kaempferol 3- O-β-glucopyranoside, quercetin 3- O-β-glucopyranoside, quercetin 3- O-6″- trans-coumaroyl-β-glucoside, kaempferol 3- O-β-rutinoside and quercetin 3- O-β-rutinoside, were isolated from the EtOAc leaf extract of Rhizophora mangle (Rhizophoraceae) combining counter-current chromatography (CCC) and gel-filtration. A solvent system of n-hexane-ethyl acetate-methanol-water (1.5:6:1.5:6) was employed at the preliminary stage of EtOAc extract fractionation as it was shown to contain compounds that differed highly in their hydrophobicity. The obtained fractions were further purified by either CCC or gel-filtration depending on their complexity. The isolated compounds were analyzed by NMR spectroscopy and the proposed structures were confirmed by HRES/ESI/TOF MS. Some of these compounds were isolated and/or identified for the first time in R. mangle.

Chemical Constituents of the Lichen Cladina macaronesica

1991 ◽  
Vol 46 (1-2) ◽  
pp. 12-18 ◽  
Author(s):  
Antonio G . González ◽  
Jaime Bermejo Barrera ◽  
Elsa Ma Rodríguez Pérez ◽  
Consuelo E. Hernández Padrón
Keyword(s):  
Natural Products ◽  
Phenolic Compounds ◽  
Silver Nitrate ◽  
Silica Gel ◽  
Column Chromatography ◽  
Chemical Constituents ◽  
Usnic Acid ◽  
Acetone Extract ◽  
First Time

Column chromatography of the acetone extract of the lichen Cladina macaronesica (Sephadex LH-20, silica gel and silver nitrate-im pregnated silica gel) afforded eight triterpenes identified by chemical and spectral m eans. α-Amyrenone, lupenone, taraxerol, taraxerone and /so-arborinol acetate were isolated for the first time from lichens and (-)-usnic acid and five mononuclear phenolic compounds were also obtained, four for the first time as natural products. The possible transformation of perlatolic acid into these phenolic compounds is briefly outlined.

scholarly journals Chemical Constituents from the Fruits ofForsythia suspensaand Their Antimicrobial Activity

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ping-Chung Kuo ◽  
Guo-Feng Chen ◽  
Mei-Lin Yang ◽  
Ya-Hua Lin ◽  
Chi-Chung Peng
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Column Chromatography ◽  
Chemical Constituents ◽  
Phenylethanoid Glycosides ◽  
Natural Sources ◽  
Methanol Extracts ◽  
Lead Compounds ◽  
Forsythia Suspensa ◽  
First Time

Lignans and phenylethanoid glycosides purified fromForsythia suspensawere reported to display various bioactivities in the previous literature, including the antimicrobial activity. Therefore, the present research is aimed to purify and identify the chemical constituents of the methanol extracts of fruits ofF. suspensa. The methanol extracts of fruits ofF. suspensawere fractionated and further purified with the assistance of column chromatography to afford totally thirty-four compounds. Among these isolates, 3β-acetoxy-20α-hydroxyursan-28-oic acid (1) was reported from the natural sources for the first time. Some of the purified principles were subjected to the antimicrobial activity examinations againstEscherichia colito explore new natural lead compounds.

scholarly journals Chemical Constituents of Egyptian Withania Somnifera Leaves and Fruits and their Anticholinesterase Activity

2019 ◽  
Vol 63 (4) ◽  
Author(s):  
Rahma S. Mahrous ◽  
Hoda M. Fathy ◽  
RASHA M. ABU EL-KHAIR ◽  
ABDALLAH A. OMAR
Keyword(s):  
Spectroscopic Data ◽  
Withania Somnifera ◽  
Vaccenic Acid ◽  
Chemical Constituents ◽  
Light Petroleum ◽  
Natural Source ◽  
Petroleum Fraction ◽  
Anticholinesterase Activity ◽  
First Report ◽  
First Time

Seven compounds were isolated from the leaves and fruits of Egyptian Withania somnifera dunal, (Family: Solanaceae). The identity of the compounds based on their spectroscopic data were as follows: two withanolides; withaperuvin C (1), phyperunolideF (2) and four lipids;1,2-di-O-palmitoyl-3-O-(6´´´-sulfo-α-D-quinovopyranosyl)-glycerol (3), vaccenic acid (5), 1,3 dicaproyl,2-vaccenoyl-glycerol (6), vaccenolymonoglyceride (7) and β-sitosterol glucoside (4). All the isolated metabolites except (4) were reported for the first time from this plant. Besides, this is the first report for isolation of compounds (6, 7) in a pure form from a natural source. Different fractions of the fruits of the Egyptian plant were investigated for their anticholinesterase activities where the most potent ones found to be the aqueous, the light petroleum fraction in addition to a mixture of lipids.

Chemical Constituents of the Aerial Parts of Scutellaria lateriflora and their α-Glucosidase Inhibitory Activities

2012 ◽  
Vol 7 (4) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Minpei Kuroda ◽  
Katsura Iwabuchi ◽  
Yoshihiro Mimaki
Keyword(s):  
Methyl Ester ◽  
Chemical Constituents ◽  
Meoh Extract ◽  
Aerial Parts ◽  
Screening Experiments ◽  
Data Analyses ◽  
Nmr Data ◽  
Scutellaria Lateriflora ◽  
Inhibitory Activities ◽  
First Time

MeOH extracts of 37 herbs were tested in screening experiments for rat intestinal α-glucosidase. The MeOH extract of the aerial parts of Scutellaria lateriflora L. (Lamiaceae) significantly inhibited sucrase and maltase activities, using sucrose and maltase as the substrates. Enzyme inhibition guided-fractionation of the MeOH extract of S lateriflora resulted in the isolation of a new diterpene glucoside, deacetylajugarin-IV 18- O-β-D-glucopyranoside (1), along with 20 known phenolics (2-21). The structures of 1-21 were elucidated on the basis of MS and NMR data analyses. Baicalein (4) and baicalin (10), a glycoside of 4, showed moderate sucrase inhibitory activities at IC50 values of 14.9 and 36.3 μM, respectively, whereas luteolin (3), acteoside (16), leucosceptoside A (18), and isoacteoside (20) showed weak inhibitory activities at IC50 values of 811, 522, 727, and 443 μM, respectively. This is the first report on mammalian α-glucosidase inhibitory activities of S lateriflora extract and identification of the constituents responsible for the activities. Apigenin (2), luteolin (3), 6-methoxyluteolin 4'-methyl ether (6), isoscutellarin 8- O-β-D-glucuronide (7), luteolin 7- O-β-D-glucuronide (9), wogonin 7- O-β-D-glucuronide methyl ester (12), eriodictyol (13), naringenin (14), naringenin 7- O-β-D-glucuronide (15), jionoside D (17), leucosceptoside A (18), and (+)-syringaresinol 4'- O-β-D-glucopyranoside (21) were isolated from this plant for the first time. The inhibitory properties of S lateriflora extract against α-glucosidase provide a prospect for its antidiabetic usage.

scholarly journals (4S)-4,8-dihydroxy-1-tetralone and other chemical constituents from Pestalotiopsis sp. EJC07, endophytic fromBauhinia guianensis

2016 ◽  
Vol 88 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Eleane M.C. de Souza ◽  
Ellon L. Da Silva ◽  
Andrey M.R. Marinho ◽  
Patrícia S.B. Marinho
Keyword(s):  
Spectral Methods ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Hydroxybenzoic Acid ◽  
Ergosterol Peroxide ◽  
First Time

The present work reports the isolation of eight compounds fromPestalotiopsis sp. EJC07 isolated as endophytic fromBauhinia guianensis, a tipical plant of the Amazon. The compounds (4S)-4,8-dihydroxy-1-tetralone (1), uracil (2), uridin (3), p-hydroxybenzoic acid (4), ergosterol (5), ergosterol peroxide (6), cerevisterol (7) and ducitol (8) were isolated by chromatographic procedures and identified by spectral methods of 1D and 2D NMR and MS. The compound 1 is being reported for the first time in the genusPestalotiopsis.

Antioxidant Constituents from the Stem and Leaf of Helicteres hirsuta Loureiro

2020 ◽  
Vol 10 ◽  
Author(s):  
Nguyen Thanh Tra ◽  
Ba Thi Cham ◽  
Nguyen Thi Thu Ha ◽  
Le Thi Tu Anh ◽  
Nguyen Van Tuyen ◽  
...  
Keyword(s):  
Rat Liver ◽  
Chemical Constituents ◽  
Positive Control ◽  
Liver Cells ◽  
Phytochemical Analysis ◽  
Etoac Extract ◽  
Mtt Method ◽  
Rat Liver Cells ◽  
First Time

Background: Helicteres hirsuta has been used traditionally as a useful agent for hepatoprotective treatment. The aim of the current study is to isolate chemical constituents from the EtOAc extract of Helicteres hirsuta stem and evaluate the capacity of the isolated compounds against hydroperoxide damaged rat liver cells Methods: Column chromatography was used for phytochemical isolation whereas MTT method was applied for intracellular antioxidative assay Results: Phytochemical analysis of the EtOAc extract of Helicteres hirsuta stem led to the isolation and determination of four triterpenoids betulin (1), bentulinic acid (2), alphitolic acid (3), and oleanolic acid (4), together with two steroids stigmast-4-ene-6β-ol-3-one (5), and β-sitostenone (6), whereas EtOAc extract of its leaf composed of three steroids cucurbitacin D (11), cucurbitacin I (12), and simiarenol (13), four flavonoids tiliroside (14), potengriffioside A (15), kaempferide (16), and isokaempferide (17), along with two carotenoids lutelin (18), and β-carotene (19). Isolated compounds 3, 5, 6, 16, and 17 were found in genus Helicteres for the first time, while carotenoids 18, and 19 were never isolated from family Sterculiaceae before. Phytochemicals derived H. hirsuta species are also useful agents for antioxidative drugs, e.g, flavonol 17 induced the significant EC50 value of 22.24 ± 0.14 μg/mL, as compared with that of positive control curcumin (EC50 19.33 ± 0.77 μg/mL), against hydroperoxide damaged rat liver cells Conclusion: Antioxidative activity of the EtOAc extract of Helicteres hirsuta stem against hydroperoxide is mostly based on the role of flavonoids.

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