scholarly journals Genetic Characterization of the Yugoslavian Shepherd Dog – Sharplanina, a Livestock Guard Dog from the Western Balkans

2020 ◽  
Vol 70 (3) ◽  
pp. 329-345
Author(s):  
Vladimir Dimitrijević ◽  
Mila Savić ◽  
Elmin Tarić ◽  
Ljubodrag Stanišić ◽  
Zoran Stanimirović ◽  
...  
Keyword(s):  
Gene Pool ◽  
Military Training ◽  
Sampling Site ◽  
Gene Pools ◽  
Western Balkans ◽  
Training Centre ◽  
Registry System ◽  
Principal Coordinates ◽  
Scaling Analyses ◽  
Foundation Stock

AbstractYugoslavian Shepherd Dog - Sharplanina (YSD) is a livestock guard dog from the Western Balkans present in this region over a long time, but recognized by the Fédération Cynologique Internationale as a distinct breed as late as 1957. However, the information regarding the origin and the size of the breed’s foundation stock is still lacking. In order to contribute towards better understanding of the genetic make-up of the YSD and its foundation stock, we re-analyzed previously generated genetic profiles of 94 registered YSD dogs assessed with nine nuclear microsatellites. Studied individuals comprised 90 unrelated dogs and two pairs of full-sibs, sampled at four sampling sites: three dog shows and at a military training centre for dogs in Serbia. We supported earlier findings on high levels of genetic diversity in YSD (HE=0.728±0.027) and lack of inbreeding, and revealed substructure of the breed because we found two distinct gene pools in the Bayesian clustering analysis, indicated also by the excess of homozygotes (i.e., Wahlund effect) and outcomes of other analyses: linkage disequilibrium tests, Neighbour-Joining tree, principal coordinates and two-dimensional scaling analyses. The two gene pools were almost equally represented at each sampling site. One gene pool was composed of individuals with high genetic integrity, while the other gene pool was characterized with admixed ancestry, developed possibly via hybridization with native breeding stock outside the registry system, other breeds, such as the Caucasian Shepherd, and/or individuals admixed with wolves. Thus, we demonstrate rather complex and diverse ancestry implying a genetically heterogeneous foundation stock of the YSD.

scholarly journals Examination of Genetic Diversity of Common Bean from the Western Balkans

2015 ◽  
Vol 140 (4) ◽  
pp. 308-316 ◽  
Author(s):  
Marko Maras ◽  
Barbara Pipan ◽  
Jelka Šuštar-Vozlič ◽  
Vida Todorović ◽  
Gordana Đurić ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Common Bean ◽  
Gene Pool ◽  
Molecular Data ◽  
Gene Pools ◽  
Western Balkans ◽  
Mesoamerican Gene Pool ◽  
Information Index ◽  
Large Clusters

In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon’s information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article.

scholarly journals Genetic diversity of common bean (Phaseolus vulgaris L.) breeding collection in Serbia

Genetika ◽  
2019 ◽  
Vol 51 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Aleksandra Savic ◽  
Milka Brdar-Jokanovic ◽  
Miodrag Dimitrijevic ◽  
Sofija Petrovic ◽  
Milan Zdravkovic ◽  
...  
Keyword(s):  
Common Bean ◽  
Gene Pool ◽  
Gene Diversity ◽  
Principal Component ◽  
Molecular Data ◽  
Phenotypic Traits ◽  
Gene Pools ◽  
Vegetable Crops ◽  
Novi Sad

The characterization of 41 common bean cultivars and landraces from breeding collection of Institute of Field and Vegetable Crops, Novi Sad, Serbia, was done based on phenotypic traits and microsatellite markers. Phenotypic traits were chosen from Bioversity International descriptor list. In addition, main yield components were investigated. Analysis of phaseolin type revealed affiliation of cultivars and landraces to Mesoamerican or Andean gene pool. Cultivars and landraces demonstrated significant diversity level with regard to studied phenotypic traits. Identified variation showed high potential for developing new cultivars with desirable combination of traits. Principal component analysis based on phenotypic traits separated bean cultivars and landraces in two groups, which corresponded to Mesoamerican and Andean determined according to phaseolin type. Putative hybrids, with combination of traits between gene pools were also identified. Analysis of microsatellite data, using twenty-two SSR primer pairs, showed medium gene diversity in studied material. Microsatellite-based cluster analysis separated genotypes in two discrete clusters and several subclusters. No clear separation according to gene pool was found between the clusters, however grouping according to gene pool and patterns of phenotypic variation, following these gene pools, were observed within subclusters. Knowledge on detailed relationships of cultivars and landraces based on phenotypic and molecular data would facilitate identification of candidates for future breeding.

The genetic make-up of the European landraces of the common bean

2011 ◽  
Vol 9 (2) ◽  
pp. 197-201 ◽  
Author(s):  
S. A. Angioi ◽  
D. Rau ◽  
L. Nanni ◽  
E. Bellucci ◽  
R. Papa ◽  
...  
Keyword(s):  
Common Bean ◽  
Gene Pool ◽  
Chloroplast Microsatellites ◽  
Gene Pools ◽  
Mesoamerican Gene Pool ◽  
Hybridization Event ◽  
South Eastern Europe ◽  
Andean Type ◽  
The Common ◽  
African Sample

Here, we present a brief overview of the main studies conducted on the common bean (Phaseolus vulgaris L.) in Europe and other countries outside its centres of origin. We focus on the proportions of the Andean and Mesoamerican gene pools, and on the inter-gene pool hybridization events. In Europe, for chloroplast microsatellites, 67% of European germplasm is of Andean origin. Within Europe, interesting trends have been seen; indeed, the majority of the Andean type is found in the three macro-areas of the Iberian Peninsula, Italy and central-northern Europe, while, in eastern and south-eastern Europe, the proportion of the Mesoamerican type increased. On a local scale, the contribution of the Mesoamerican type is always low. On other continents, various situations are seen using different markers: in China and Brazil, the Mesoamerican gene pool prevails, while in an African sample, overall, both gene pools are equally represented, with differences in individual countries. The frequency of European bean genotypes deriving from at least one hybridization event was 44% with an uneven distribution. Interestingly, hybrids tend to have intermediate seed size in comparison with ‘pure’ Andean or Mesoamerican types. On other continents, very few hybrids are found, probably because of the different marker systems used.

Vitamin C supplementation reduces the odds of developing a common cold in Republic of Korea Army recruits: randomised controlled trial

2020 ◽  
pp. bmjmilitary-2019-001384 ◽  
Author(s):  
Tae Kyung Kim ◽  
H R Lim ◽  
J S Byun
Keyword(s):  
Placebo Group ◽  
Vitamin C ◽  
Common Cold ◽  
Respiratory Diseases ◽  
Military Training ◽  
Multiple Logistic Regression Analysis ◽  
Republic Of Korea ◽  
Training Centre ◽  
Vitamin C Supplementation ◽  
Army Soldiers

IntroductionThe Republic of Korea (ROK) military has a high incidence of respiratory diseases at training centres. Vitamin C has been reported to reduce the incidence of colds. For the purpose of preventing soldiers' respiratory diseases, this study aimed to investigate whether vitamin C intake can prevent common colds in the ROK Army soldiers.MethodsThis was a randomised, placebo-controlled, and double-blind trial of soldiers who enlisted in the Korea Army Training Centre for 30 days from 12 February to 13 March 2018. The study participants were divided into groups (vitamin C vs placebo). The military medical records were searched to determine whether the participants had a common cold. Multiple logistic regression analysis was performed to identify the association between vitamin C intake and diagnosis of common colds. In addition, subgroup analysis on the relationship between vitamin C intake and common cold according to smoking status, training camp and physical rank was conducted.ResultsA total of 1444 participants were included in our study. Of these participants, 695 received vitamin C (6000 mg/day, vitamin C group), while 749 participants received placebo (0 mg/day, placebo group). The vitamin C group had a 0.80-fold lower risk of getting a common cold than did the placebo group. Subgroup analyses showed that this effect was stronger among subjects in camp A, among never smokers and among those in physical rank 3.ConclusionVitamin C intake provides evidence to suggest that reducing the common colds in Korean Army soldiers. Our results may serve as a basis for introducing military healthcare policies that can provide vitamin C supplementation for military personnel in basic military training.

Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites

2011 ◽  
Vol 9 (01) ◽  
pp. 86-96 ◽  
Author(s):  
Lucy M. Díaz ◽  
Héctor F. Buendía ◽  
Myriam C. Duque ◽  
Matthew W. Blair
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Common Bean ◽  
Gene Pool ◽  
Major Gene ◽  
Gene Pools ◽  
Silver Stain ◽  
Fluorescent Marker ◽  
Fluorescent Markers

Colombia, situated at the northern end of the Andes mountains of South America and in proximity to Central America, is an important centre of diversity for common bean (Phaseolus vulgarisL.) that has a mix of cultivated germplasm from both major gene pools (Andean and Mesoamerican) for the species. Microsatellites are a useful marker system for analyzing genetic diversity of this crop and can be analyzed with manual (silver-stain) or automated (ABI) detection systems and using unlabelled or fluorescently labelled markers, respectively. The objectives of this research were to evaluate the genetic diversity of 92 Colombian landraces and gene pool controls with 36 fluorescent and 30 non-fluorescent microsatellite markers and to determine the extent of introgression between the Andean and Mesoamerican gene pools for this germplasm. A comparison of fluorescentversusnon-fluorescent marker systems was performed with 14 loci, which were evaluated with both methods; the fluorescent markers were found to be more precise than the non-fluorescent markers in determining population structure. A combined analysis of 52 microsatellites using the 36 fluorescent markers and 16 non-overlapping, silver-stained markers produced an accurate population structure for the Andean gene pool that separated race Nueva Granada and race Peru genotypes and clearly identified introgression between these races and the gene pools. The results of this research are important for the application of microsatellite markers to diversity analysis in common bean and for the conservation of landraces in Colombia and neighbouring countries of Latin America, where similar germplasm exists and where gene pool or race mixtures also occur.

Attentional and mnemonic deficits associated with infectious disease in humans

1999 ◽  
Vol 29 (2) ◽  
pp. 291-297 ◽  
Author(s):  
L. CAPURON ◽  
D. LAMARQUE ◽  
R. DANTZER ◽  
G. GOODALL
Keyword(s):  
Infectious Disease ◽  
Human Subjects ◽  
Military Training ◽  
Cognitive Test ◽  
Necessary Condition ◽  
Cross Sectional ◽  
Everyday Memory ◽  
Training Centre ◽  
Cognitive Disturbances ◽  
Cross Sectional Design

Background. Infectious diseases are accompanied by behavioural and psychological changes that suggest the implication of the central nervous system. Among them, cognitive alterations have been reported, but their specificity and implication in everyday life are still largely unclear. The purpose of the present study was to evaluate and specify the everyday memory disturbances in sick human subjects and to determinate the role of fever in the appearance of these alterations.Methods. The study was carried out in a military training centre for naval recruits. Ninety-one volunteer subjects, healthy (N=30) or suffering from flu-like syndrome, with (N=29) or without fever (N=32), participated in this experiment and were administered a cognitive test (the ERBMT) according to a cross-sectional design for assessing various aspects of everyday memory.Results. Sick subjects were specifically impaired in daily memory tasks that require the temporary management of a large amount of information. This impairment was similar for the feverish and apyretic sick subjects who both differed from the controls.Conclusion. These findings suggest that infectious disease disturbs the complex cognitive processes that might be associated with attentional functions. Moreover, these results show that fever is not a necessary condition for the appearance of these cognitive disturbances.

RFLP diversity of common bean (Phaseolus vulgaris) in its centres of origin

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Viviana L. Becerra Velasquez ◽  
Paul Gepts
Keyword(s):  
Genetic Diversity ◽  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Gene Pool ◽  
Geographical Area ◽  
Rflp Markers ◽  
Gene Pools ◽  
Restriction Fragment Length

Eighty-five wild and cultivated accessions of common bean (Phaseolus vulgaris L.), representing a wide geographic area in the centres of domestication were tested for restriction fragment length polymorphisms (RFLPs). Genomic DNA was digested with one of three restriction enzymes (EcoRI, EcoRV, and HindIII) and hybridized to 12 probes distributed throughout the common bean genome. Accessions could be classified into two major groups with a distinct geographical distribution in Middle America and the Andes. Within each gene pool, cultivated accessions clustered together with wild forms from the same geographical area supporting the multiple domestications hypothesis for this crop. Estimates of Nei's genetic distances among the cultivated races from the two different gene pools varied from 0.12 to 0.56 and among races from the same gene pool from 0.04 to 0.12, suggesting that the divergence in Phaseolus vulgaris has reached the subspecies level. The level of genetic diversity (Ht = 0.38) was twice the value obtained with isozyme analysis. Genetic diversity within races (Hs = 0.27) was four to five times higher compared with isozymes, but genetic diversity between races (Dst = 0.11) was similar for both categories of markers. These results corroborate previous studies on the characterization of genetic diversity in common bean that clearly showed two distinct gene pools, Middle American and Andean. Moreover, RFLP markers are superior to isozymes because they provide better coverage of the genome and reveal higher level of polymorphisms.Key words: common bean, restriction fragment length polymorphism, domestication, genetic diversity.

scholarly journals The five Urochola spp. used in development of tropical forage cultivars originate from defined subpopulations with differentiated gene pools

2021 ◽  
Author(s):  
Janet Higgins ◽  
Paulina Tomaszewska ◽  
Till K Pellny ◽  
Valheria Castiblanco ◽  
Jacobo Arango ◽  
...  
Keyword(s):  
Gene Pool ◽  
Geographical Origin ◽  
Snp Markers ◽  
Gene Pools ◽  
Rna Seq ◽  
Genome Composition ◽  
Ploidy Levels ◽  
Centre Of Origin ◽  
Tropical Forage ◽  
The Tropics

Background and Aims: Urochola (syn. Brachiaria, and including some Panicum and Megathyrus) is a genus of tropical and subtropical grasses widely sown as forage to feed ruminants in the tropics. A better understanding of the diversity among Urochola spp. allow us to leverage its varying ploidy levels and genome composition to accelerate its improvement, following the example from other crop genera. Methods: We explored the genetic make-up and population structure in 111 accessions, which comprise the five Urochola species used for the development of commercial cultivars. These accessions are conserved from wild materials from collection sites at their centre of origin in Africa. We used RNA-seq, averaging 40M reads per accession, to generate 1,167,542 stringently selected SNP markers that tentatively encompassed the complete Urochola gene pool used in breeding. Key Results: We identified ten subpopulations, which had no relation with geographical origin and represented ten independent gene pools, and two groups of admixed accessions. Our results support a division in U. decumbens by ploidy, with a diploid subpopulation closely related to U. ruziziensis, and a tetraploid subpopulation closely related to U. brizantha. We observed highly differentiated gene pools in U. brizantha, which were not related with origin or ploidy. Particularly, one U. brizantha subpopulation clustered distant from the other U. brizantha and U. decumbens subpopulations, so likely containing unexplored alleles. We also identified a well-supported subpopulation containing both polyploid U. decumbens and U. brizantha accessions; this was the only group containing more than one species and tentatively constitutes an independent "mixed" gene pool for both species. We observed two gene pools in U. humidicola. One subpopulation, "humidicola-2", was much less common but likely includes the only known sexual accession in the species. Conclusions: Our results offered a definitive picture of the available diversity in Urochola to inform breeding and resolve questions raised by previous studies. It also allowed us identifying prospective founders to enrich the breeding gene pool and to develop genotyping and genotype-phenotype association mapping experiments.

scholarly journals INCREASING RAPE SEED YIELDS BY THE WAY OF HETEROSIS SELECTION

2021 ◽  
Author(s):  
Yu. D. Syrova ◽  
Keyword(s):  
Gene Pool ◽  
Estimation Methods ◽  
Gene Pools ◽  
Rape Seed ◽  
Heterosis Effect ◽  
Winter Rapeseed ◽  
Seed Yields ◽  
The Way

Usage of heterosis effect in rapeseed became possible after plenty experiments which proved existence of the significant heterosis. A level of heterosis in spring rapeseed hybrids varied from 20 to 50 %, in winter rapeseed hybrids – from 20 to 80 %. A meaning of heterosis in rapeseed depended on the estimation methods, environments, gene pool diversity. Scientists do their best to create alternative gene pools using in part method of rapeseed resynthesis.

scholarly journals Genetic structure of ural populations of Larix sibirica Ledeb. on the base of analysis of nucleotide polymorphism

2020 ◽  
Vol 69 (1) ◽  
pp. 20-28 ◽  
Author(s):  
Yu. S. Vasilyeva ◽  
A. A. Zhulanov ◽  
S. V. Boronnikova ◽  
Yu. A. Yanbaev
Keyword(s):  
Genetic Structure ◽  
Gene Pool ◽  
Large Population ◽  
Haplotype Diversity ◽  
Larix Sibirica ◽  
Fixation Index ◽  
Gene Pools ◽  
Nucleotide Polymorphism ◽  
Term Survival ◽  
Key Species

AbstractResearch on the state and dynamics of the gene pool (an important natural resource that determines the potential fitness of living organisms and, ultimately, their long-term survival) becomes an important problem in the context of increased anthropogenic environmental impact. They are especially important for key species of ecosystems of a global scale importance. Larix sibirica Ledeb., which spreads from the Western Siberia to the Russian North-West, is one of such forest tree species. We identified patterns of genetic structure of populations on the example of the species’ Western race on the Middle and Northern Urals. The analysis of nucleotide polymorphism of genes of ABA-inducible protein, MADS-box-transcription factor and of 4-kumarat: CoA ligase (a part of the gene) was used as a method. Evidences were obtained that a part of the populations previously formed a single large population. At the same time, populations with different gene pools were found. As a result, differences between populations within the region were more pronounced (fixation index FST = -0.021 – 0.260, total haplotype diversity Hd = 0.636 – 0.911; nucleotide diversity π = 0.005 – 0.009; number of mutations θW = 0.005 – 0.012) than in other parts of the race. Causes of this phenomenon are discussed. It was concluded that the larch forests with a unique gene pool and/or high genetic diversity should be objects of population-oriented forestry and conservation.

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