scholarly journals Co-Infection of Bovine Papular Stomatitis Virus, Rotavirus and Cryptosporidium Spp. In a Calf

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Eroksuz Yesari ◽  
Timurkan Mehmet Özkan ◽  
Abayli Hasan ◽  
Incili Canan Akdeniz ◽  
Karabulut Burak ◽  
...  
Keyword(s):  
Foot And Mouth Disease ◽  
Polymerase Chain Reaction Analysis ◽  
Virus Infections ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Viral Diarrhea ◽  
Cryptosporidium Spp ◽  
Bovine Papular Stomatitis Virus ◽  
Polymerase Chain ◽  
First Time

AbstractConcurrent occurence of bovine papular stomatitis, rotavirus infection and cryptosporidiosis was diagnosed postmortem in a 7-days-old calf from a farm containing 65 calves of different ages. Multifocal papular stomatitis and rumenitis were present on necropsy. While polymerase chain reaction analysis revealed rotavirus and papular stomatitis virus infections; bovine viral diarrhea, foot and mouth disease, bovine papilloma virus and coronavirus could not be detected. Overall; concurrent co-infection with bovine papular stomatitis virus, rotavirus and cryptosporidium spp. was reported for the first time.

scholarly journals Central Nervous System Infection Diagnosis by Next-Generation Sequencing: A Glimpse Into the Future?

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Nguyen Thi Hoang Mai ◽  
Nguyen Hoan Phu ◽  
Le Nguyen Truc Nhu ◽  
Nguyen Thi Thu Hong ◽  
Nguyen Ho Hong Hanh ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Deep Sequencing ◽  
Central Nervous System Infection ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Life Threatening ◽  
Polymerase Chain ◽  
First Time ◽  
Generation Sequencing

Abstract Japanese encephalitis virus was detected by deep sequencing for the first time in urine of a 16-year-old boy with encephalitis. Seroconversion and polymerase chain reaction analysis confirmed the metagenomics finding. Urine is useful for diagnosis of flaviviral encephalitis, whereas deep sequencing can be a panpathogen assay for the diagnosis of life-threatening infectious diseases.

scholarly journals A New Disease in Limonium latifolium Hybrids. II. Investigating Insect Vectors

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 1060-1061 ◽  
Author(s):  
Phyllis G. Weintraub ◽  
Shimon Pivonia ◽  
Arieh Rosner ◽  
Abed Gera
Keyword(s):  
Polymerase Chain Reaction Analysis ◽  
New Disease ◽  
Chain Reaction ◽  
Large Numbers ◽  
Circulifer Haematoceps ◽  
Laboratory Colony ◽  
Phytoplasma Infection ◽  
Polymerase Chain ◽  
Phytoplasma Vector ◽  
First Time

Symptoms typical of a phytoplasma infection were observed on a large number of Limonium hybrids in Israel in October 2000. Phytoplasma infection was confirmed by electron microscopy and polymerase chain reaction analysis. To identify the agent of pathogen spread surveys were made of candidate leafhoppers vectors in Limonium hybrid crops: one known phytoplasma vector (Orosius orientalis (Matsumura)) was present in very large numbers, ≈10-fold greater than any other species; three other known vectors were present in low to moderate numbers (Circulifer haematoceps complex (Mulsant et Rey), C. tenellus complex (Baker) and Exitianus capicola Stål); individual specimens of other vectors were occasionally found. Field collected specimens of these four species were shown to vector phytoplasma to healthy Limonium hybrid seedlings; this is the first time E. capicola has been shown to be a phytoplasma vector. A laboratory colony of O. orientalis was additionally shown to be able to acquire the phytoplasma from infected Limonium hybrids and subsequently vector it to healthy seedlings.

scholarly journals First isolation of dengue 3 in Brazil from an imported case

2001 ◽  
Vol 43 (1) ◽  
pp. 55-57 ◽  
Author(s):  
Iray Maria ROCCO ◽  
Berenice Bustamanti KAVAKAMA ◽  
Cecília Luiza Simões SANTOS
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Isolation And Identification ◽  
Susceptible Population ◽  
Imported Case ◽  
Orbital Pain ◽  
Polymerase Chain ◽  
And Control ◽  
First Time

The authors report the isolation of dengue 3 virus for the first time in Brazil. The patient, resident in Limeira-SP, traveled to Nicaragua on May 16th, 1998, where he stayed for two months. Starting on August 14th he had fever, headache, myalgia, arthralgia, retro-orbital pain and diarrhea. He returned to Brazil on August 16th and was hospitalized in the next day. The patient had full recovery and was discharged on August 20th. The virus was isolated in C6/36 cell culture inoculated with serum collected on the 6th day after the onset of the symptoms. The serotype 3 was identified by indirect immunofluorescence assays performed with type-specific monoclonal antibodies. This serotype was further confirmed by polymerase chain reaction analysis. The introduction of a new dengue serotype in a susceptible population is a real threat for the occurrence of severe forms of the disease. The isolation and identification of dengue virus are important in order to monitoring the serotypes circulating in Brazil and to take the measures necessary to prevent and control an epidemic.

Detection of Genital Human Papilloma Virus Infections by the Polymerase Chain Reaction

1991 ◽  
pp. 326-338
Author(s):  
J. M. M. Walboomers ◽  
A. J. C. Van Den Brule ◽  
P. J. F. Snijders ◽  
M. Du Maine ◽  
P. Kenemans ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Virus Infections ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain
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