scholarly journals Ultrastructure and cytochemistry of the mature spermatozoon of Khawia armeniaca (Cholodkovsky, 1915) (Caryophyllidea: Lytocestidae), a parasite of Capoeta capoeta sevangi (De Filippi, 1865) (Teleostei, Cyprinidae)

2020 ◽  
Vol 57 (4) ◽  
pp. 353-360
Author(s):  
M. Matoušková ◽  
M. Bruňanská ◽  
J. Nebesářová ◽  
L. G. Poddubnaya
Keyword(s):  
Anterior Part ◽  
Male Gamete ◽  
Longitudinal Axis ◽  
Mature Spermatozoon ◽  
Ultrastructural Organization ◽  
Cortical Microtubules ◽  
Transmission Electron ◽  
Cytochemical Technique ◽  
First Time ◽  
Region Ii

SummaryThe mature spermatozoon of Khawia armeniaca, a monozoic caryophyllidean parasite of templar fish Capoeta capoeta sevangi (De Filippi, 1865) from the Lake Sevan, Armenia, has been studied using transmission electron microscopy and cytochemical technique of Thiéry (1967) for the first time. The mature spermatozoon of K. armeniaca consists of a single axoneme with the 9+‘1’ trepaxonematan structure, cortical microtubules and nucleus which are situated parallel to the longitudinal axis of the spermatozoon, and a moderately electrondense cytoplasm with glycogen particles. The cortical microtubules are arranged in one continuous semicircle beneath the plasma membrane in Region II and anterior part of Region III of the mature spermatozoon. The two opposite rows of cortical microtubules are observed in the remaining nuclear and at the beginning of the postnuclear part (Regions III, IV) of the male gamete The number of cortical microtubules is remarkably variable in the spermatozoa of various Khawia species. K. armeniaca exhibits the highest number of cortical microtubules in comparison with K. sinensis and K. rossittensis. Glycogen was detected in the cytoplasm of prenuclear (II), nuclear (III) and postnuclear (IV) regions with different ultrastructural organization of the mature spermatozoon of K. armeniaca. Variations of sperm ultrastructural characters within caryophyllideans and other cestodes are discussed.

scholarly journals Spermatozoon ultrastructure in two monorchiid digeneans

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2488 ◽  
Author(s):  
Yann Quilichini ◽  
Abdoulaye J.S. Bakhoum ◽  
Jean-Lou Justine ◽  
Rodney A. Bray ◽  
Cheikh T. Bâ ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Male Gamete ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
General Morphology ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
First Time ◽  
External Ornamentation ◽  
Phylogenetical Relationship

Spermatological characteristics of species from two monorchiid genera,OpisthomonorchisandParamonorcheides, have been investigated, for the first time, by means of transmission electron microscopy. The ultrastructural study reveals that the mature spermatozoon ofOpisthomonorchis dinemaandParamonorcheides selarisshare several characters such as the presence of two axonemes of different lengths showing the 9+“1” pattern of the Trepaxonemata, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies, granules of glycogen and similar morphologies of the anterior and posterior extremities. The slight differences between the male gamete ofO. dinemaandP. selarisare the length of the first axoneme and the position of the second mitochondrion. This study also elucidates the general morphology of the spermatozoon in all monorchiid species described so far, which corresponds to a unique spermatozoon type. Other interesting finds concern the spermatological similarities between monorchiid spermatozoa and the mature spermatozoon reported in the apocreadiidNeoapocreadium chabaudi. These similarities allow us to suggest a close phylogenetical relationship between the Monorchiidae and the Apocreadiidae, although more studies are needed, especially in the unexplored taxa.

scholarly journals Ultrastructure of the mature spermatozoon of Eubothrium rugosum (Batsch, 1786) with a re-assessment of the spermatozoon ultrastructure of Eubothrium crassum (Bloch, 1779) (Cestoda: Bothriocephalidea)

2010 ◽  
Vol 47 (4) ◽  
pp. 257-263 ◽  
Author(s):  
M. Bruňanská ◽  
H.-P. Fagerholm ◽  
J. Nebesářová ◽  
B. Kostič
Keyword(s):  
Posterior Extremity ◽  
Posterior Part ◽  
Mature Spermatozoon ◽  
Structural Polymorphism ◽  
Dense Granules ◽  
Male Gametes ◽  
Transmission Electron ◽  
Anterior Ring ◽  
First Time ◽  
Region Ii

AbstractThe ultrastructure of the mature spermatozoon of the bothriocephalidean tapeworm Eubothrium rugosum, a parasite of the burbot, Lota lota (L.), was studied by transmission electron microscopy for the first time. In addition, spermatozoon ultrastructure of Eubothrium crassum has been re-assessed. New is the finding, that the mature spermatozoa of both species of the genus Eubothrium exhibit essentially the same general morphology. They are filiform cells tapering at both extremities, and they possess the two axonemes with 9+“1” pattern of Trepaxonemata, attachment zones, a nucleus, cortical microtubules (CMs), electron-dense granules, and a single crested body. Structural polymorphism of the CBs has been found within the two Eubothrium species for the first time. The anterior ring of electron-dense tubular structures surrounding a single axoneme marks the border between the two defined regions, region I and region II of the spermatozoon. This unique feature has only been observed in the Bothriocephalidea. The anuclear axoneme region II of Eubothrium spermatozoa fluently verges into a nuclear region III. The posterior part of the spermatozoon contains one-axoneme, few CMs and a posterior extremity of the nucleus that subsequently disappears. The posterior extremity of the male gametes of the genus Eubothrium exhibits elements of a disorganized axoneme which characterize also spermatozoa of the family Triaenophoridae. Discussed are interspecific similarities and differences between the spermatozoa of the two Eubothrium species as well as between these and other Eucestoda.

Spermiogenesis and spermatozoon of the tapeworm Parabothriocephalus gracilis (Bothriocephalidea): Ultrastructural and cytochemical studies

2010 ◽  
Vol 55 (1) ◽  
Author(s):  
Lenka Šípková ◽  
Céline Levron ◽  
Mark Freeman ◽  
Tomáš Scholz
Keyword(s):  
Electron Microscopy ◽  
Anterior Extremity ◽  
Mature Spermatozoon ◽  
Dense Material ◽  
Apical Region ◽  
Cortical Microtubules ◽  
Electron Dense Material ◽  
Dense Granules ◽  
Cytoplasmic Extension ◽  
Transmission Electron

AbstractSpermiogenesis and spermatozoon ultrastructure of the tapeworm Parabothriocephalus gracilis were described using transmission electron microscopy (TEM). Spermiogenesis is characterized by the formation of a zone of differentiation with two centrioles associated with striated rootlets, and an intercentriolar body between them. The two flagella undergo a rotation of 90° until they become parallel to the median cytoplasmic extension with which they fuse. Electron-dense material is present in the apical region of the zone of differentiation in the early stages of spermiogenesis. This electron-dense material is characteristic for the orders Bothriocephalidea and Diphyllobothriidea. The mature spermatozoon contains two axonemes of the 9 + ‘1’ trepaxonematan pattern, nucleus, parallel cortical microtubules and electron-dense granules of glycogen. The anterior extremity of the spermatozoon exhibits a single helical electron-dense crested body 130 nm thick. One of the most interesting features is the presence of a ring of cortical microtubules surrounding the axoneme. This character has been reported only for species of the order Bothriocephalidea and may be unique in this cestode group.

scholarly journals The Centriolar Adjunct–Appearance and Disassembly in Spermiogenesis and the Potential Impact on Fertility

Cells ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 180 ◽  
Author(s):  
Anastasiia S. Garanina ◽  
Irina B. Alieva ◽  
Elizaveta E. Bragina ◽  
Emmanuelle Blanchard ◽  
Brigitte Arbeille ◽  
...  
Keyword(s):  
Average Length ◽  
Chromatin Condensation ◽  
Mature Spermatozoon ◽  
Section Plane ◽  
Parallel Orientation ◽  
Transmission Electron ◽  
Healthy Donors ◽  
Potential Impact ◽  
First Time ◽  
Porcine Spermatozoa

During spermiogenesis, the proximal centriole forms a special microtubular structure: the centriolar adjunct. This structure appears at the spermatid stage, which is characterized by a condensed chromatin nucleus. We showed that the centriolar adjunct disappears completely in mature porcine spermatozoa. In humans, the centriolar adjunct remnants are present in a fraction of mature spermatids. For the first time, the structure of the centriolar adjunct in the cell, and its consequent impact on fertility, were examined. Ultrastructural analysis using transmission electron microscopy was performed on near 2000 spermatozoa per person, in two patients with idiopathic male sterility (IMS) and five healthy fertile donors. We measured the average length of the “proximal centriole + centriolar adjunct” complex in sections, where it had parallel orientation in the section plane, and found that it was significantly longer in the spermatozoa of IMS patients than in the spermatozoa of healthy donors. This difference was independent of chromatin condensation deficiency, which was also observed in the spermatozoa of IMS patients. We suggest that zygote arrest may be related to an incompletely disassembled centriolar adjunct in a mature spermatozoon. Therefore, centriolar adjunct length can be potentially used as a complementary criterion for the immaturity of spermatozoa in the diagnostics of IMS patients.

scholarly journals Spermatological characters of Acanthobothrium crassicolle Wedl, 1855 (Tetraphyllidea, Onchobothriidae), a parasite of the common stingray Dasyatis pastinaca

2011 ◽  
Vol 56 (2) ◽  
Author(s):  
Adji Marigo ◽  
Catarina Eira ◽  
Cheikh Bâ ◽  
Jordi Miquel
Keyword(s):  
General Pattern ◽  
Male Gamete ◽  
Ultrastructural Organization ◽  
Dense Layer ◽  
Cytoplasmic Process ◽  
Dense Granules ◽  
Transmission Electron ◽  
Apical Cone ◽  
Flagellar Rotation ◽  
Means Of Transmission

AbstractWe describe the spermiogenesis process and the ultrastructural characters of the spermatozoon of Acanthobothrium crassicolle by means of transmission electron microscopy, including cytochemical analysis for glycogen. Spermiogenesis in A. crassicolle begins with the formation of the differentiation zone that contains two centrioles associated with striated rootlets and an intercentriolar body. The latter is formed by one electron-dense layer. The centrioles develop into two free flagella that first grow orthogonally to a median cytoplasmic process and then undergo flagellar rotation becoming parallel to that median cytoplasmic process. After flagellar rotation only one of the flagella completes its growth and both short and long flagella undergo proximodistal fusion with the median cytoplasmic process. In the final stages of spermiogenesis, the nucleus becomes filiform and migrates into the spermatid body. Later, the ring of arched membranes constricts and the spermatozoon is liberated from the residual cytoplasm. The ultrastructural organization of the spermatozoon of A. crassicolle follows the general pattern of spermatozoa of the other Tetraphyllidea-Onchobothriidae species, but exhibits some differences. It is filiform, tapered at both extremities and lacks mitochondrion. It contains two axonemes of unequal length showing the 9 + “1“ pattern of Trepaxonemata, a nucleus, parallel cortical microtubules and electron-dense granules of glycogen. The anterior extremity of the male gamete contains a single crested body surrounding a thin and long apical cone. This type of apical cone has never been described in a tetraphyllidean spermatozoon. Another particularity is the presence of a single electron-dense microtubule at the vertex of the crested body.

scholarly journals Spermiogenesis and ultrastructure of the spermatozoon of Wardula capitellata (Digenea, Mesometridae), an intestinal parasite of the sparid teleost Sarpa salpa in Senegal

2012 ◽  
Vol 57 (1) ◽  
Author(s):  
Abdoulaye Bakhoum ◽  
Papa Ndiaye ◽  
Aminata Sène ◽  
Cheikh Bâ ◽  
Jordi Miquel
Keyword(s):  
Plasma Membrane ◽  
Intestinal Parasite ◽  
Mature Spermatozoon ◽  
Cytoplasmic Process ◽  
Cortical Microtubules ◽  
Differentiation Zone ◽  
Sarpa Salpa ◽  
First Time ◽  
External Ornamentation ◽  
Free Flagellum

AbstractThe spermiogenesis process in Wardula capitellata begins with the formation of a differentiation zone containing two centrioles associated with striated rootlets and an intercentriolar body. Each centriole develops into a free flagellum orthogonal to a median cytoplasmic process. Later these flagella rotate and become parallel to the median cytoplasmic process, which already exhibits two electron-dense areas and spinelike bodies before its proximodistal fusion with the flagella. The final stage of the spermiogenesis is characterized by the constriction of the ring of arched membranes, giving rise to the young spermatozoon, which detaches from the residual cytoplasm. The mature spermatozoon of W. capitellata presents most of the classical characters reported in digenean spermatozoa such as two axonemes of different lengths of the 9 + “1” trepaxonematan pattern, nucleus, mitochondrion, two bundles of parallel cortical microtubules and granules of glycogen. However, some peculiarities such as two lateral expansions accompanied by external ornamentation of the plasma membrane and spinelike bodies characterize the mature sperm. Moreover, a new spermatological character is described for the first time, the so-called cytoplasmic ornamented buttons.

scholarly journals Ultrastructure of mature spermatozoa of three Bucephalidae (Prosorhynchus longisaccatus, Rhipidocotyle khalili and Bucephalus margaritae) and phylogenetic implications

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 65 ◽  
Author(s):  
Papa Ibnou Ndiaye ◽  
Bernard Marchand ◽  
Cheikh Tidiane Bâ ◽  
Jean-Lou Justine ◽  
Rodney A. Bray ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Anterior Extremity ◽  
Posterior Extremity ◽  
Anterior Part ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Lateral Expansion ◽  
Phylogenetic Implications ◽  
External Ornamentation ◽  
Mature Spermatozoa

We describe here the mature spermatozoa of three species of bucephalids, namely Bucephalus margaritae, Rhipidocotyle khalili and Prosorhynchus longisaccatus. This study provides the first ultrastructural data on the genera Bucephalus and Rhipidocotyle and enabled us to confirm the model of the mature spermatozoon in the Bucephalinae. The spermatozoon exhibits two axonemes with the 9 + “1” pattern of the Trepaxonemata, one of which is very short, lateral expansion, external ornamentation of the plasma membrane located in the anterior extremity of the spermatozoon and associated with cortical microtubules, spine-like bodies, a mitochondrion, and a nucleus. The maximum number of cortical microtubules is located in the anterior part of the spermatozoon. However, more studies are needed to elucidate if spine-like bodies are present in all the Bucephalinae or not. In the Prosorhynchinae, the mature spermatozoon exhibits a similar ultrastructural pattern. Some differences are observed, particularly the axoneme lengths and the arrangement of the spine-like bodies. The posterior extremity of the spermatozoon in the Bucephalinae exhibits only the nucleus, but prosorhynchines have microtubules.

scholarly journals A comparative study of the ultrastructural characteristics of the mature spermatozoa of two fellodistomids Tergestia clonacantha and T. laticollis and contribution to the phylogenetic knowledge of the Gymnophalloidea

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 67
Author(s):  
Papa Ibnou Ndiaye ◽  
Bernard Marchand ◽  
Cheikh Tidiane Bâ ◽  
Jean-Lou Justine ◽  
Rodney Alan Bray ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
New Caledonia ◽  
General Pattern ◽  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Transmission Electron ◽  
Ultrastructural Model ◽  
Ultrastructural Characteristics ◽  
External Ornamentation ◽  
Mature Spermatozoa

The ultrastructure of the mature spermatozoa of Tergestia clonacantha and T. laticollis collected from the digestive tracts of fishes from New Caledonia is described using transmission electron microscopy and compared to that of related species. The spermatozoa of the two species exhibit the general pattern described in most digeneans, namely two axonemes with the 9 + “1” pattern of the Trepaxonemata, nucleus, mitochondrion, cortical microtubules, an external ornamentation of the plasma membrane, spine-like bodies and granules of glycogen. The spermatozoa of T. clonacantha and T. laticollis show the same ultrastructural model with some specificities in each case, particularly in the disposition of the structures in the posterior extremities of the spermatozoon. This study confirms that ultrastructural characters of the mature spermatozoon are useful tools for the phylogenetic analysis of the Digenea.

Ultrastructure of spermiogenesis and mature spermatozoon of Skrjabinoporus merops (Cyclophyllidea, Metadilepididae)

2006 ◽  
Vol 51 (3) ◽  
Author(s):  
Aneta Yoneva ◽  
Katia Georgieva ◽  
Yana Mizinska ◽  
Boyko Georgiev ◽  
Stoyanka Stoitsova
Keyword(s):  
Proximal Part ◽  
Mature Spermatozoon ◽  
Differentiation Zone ◽  
Region I ◽  
The Family ◽  
Cytoplasmic Protrusion ◽  
Flagellar Rotation ◽  
First Time ◽  
Region Ii ◽  
Free Flagellum

AbstractThe ultrastructure of the mature spermatozoon and the spermiogenesis of a cestode belonging to the family Metadilepididae is described for the first time. The mature spermatozoon of Skrjabinoporus merops is characterized by twisted peripheral microtubules, the presence of a single crested body, periaxonemal sheath and electron-dense rods, and the absence of intracytoplasmic walls and inclusions (glycogen or proteinaceous granules); no peripheral microtubules where nucleus contacts the external plasma membrane. Four morphologically distinct regions of the mature spermatozoon are differentiated. The proximal part (Region I) contains a single crested body, periaxonemal sheath is absent in some (proximal) sections and is present in others situated closer to the nucleus. The central Region II is nucleated, and is followed by Region III that contains a periaxonemal sheath. The distal pole, Region IV, is characterized by disintegration of the axoneme. Spermiogenesis follows the type III pattern (Bâ and Marchand 1995) although in S. merops a slight flagellar rotation is observed. The differentiation zone is characterized by the absence of striated roots and intercentriolar body; two centrioles are present, one of which gives rise to a free flagellum. The latter rotates and undergoes proximodistal fusion with the cytoplasmic protrusion of the differentiation zone. Spermiological characters of S. merops are similar to those of the families Taeniidae and Catenotaeniidae. The mature spermatozoon differs from those of the Dilepididae (where the metadilepidid species have previously been classified) by the lack of glycogen.

Ultrastructure of spermiogenesis and mature spermatozoon of Angularella beema (Clerc, 1906) (Cestoda, Cyclophyllidea, Dilepididae)

2006 ◽  
Vol 51 (4) ◽  
Author(s):  
Aneta Yoneva ◽  
Jordi Miquel ◽  
Zdzisław Świderski ◽  
Katia Georgieva ◽  
Yana Mizinska ◽  
...  
Keyword(s):  
Mature Spermatozoon ◽  
Cortical Microtubules ◽  
Cestode Species ◽  
Family Presence ◽  
Differentiation Zone ◽  
Type Iv ◽  
The Family ◽  
Cytoplasmic Protrusion ◽  
Ultrastructural Characteristics ◽  
First Time

AbstractThe ultrastructure of the spermiogenesis of a dilepidid cestode species is described for the first time. The spermiogenesis of Angularella beema is characterised by absence of both flagellar rotation and proximodistal fusion. The differentiation zone is surrounded by cortical microtubules and is delimited by a ring of arching membranes. It contains two centrioles, one of which develops the axoneme that grows directly into the elongating cytoplasmic protrusion. This pattern of spermiogenesis was described as the Type IV spermiogenesis of cestodes. Among cestodes, similar pattern of spermiogenesis is known in the family Hymenolepididae and in some representatives of the family Anoplocephalidae. The mature spermatozoon of A. beema consists of five regions differing in their ultrastructural characteristics. It is characterised by the presence of cortical microtubules (spirally arranged at angle of 30–40° to the spermatozoon axis) and a single crested body. There is a periaxonemal sheath in certain parts of the spermatozoon as well as glycogen-like granules between the periaxonemal sheath and the cortical microtubules. The comparisons of the mature spermatozoon of A. beema with those of other two dilepidid species (Dilepis undula and Molluscotaenia crassiscolex) demonstrate some variation within the family: presence of periaxonemal sheath in A. beema and D. undula and its absence in M. crassiscolex; presence of electron-dense rods in D. undula and their absence in A. beema.

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