scholarly journals Influence of Microbiologically Enriched Mineral Fertilizers on Selected Groups of Microorganisms in the Rhizosphere of Strawberry Plants

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Lidia Sas-Paszt ◽  
Urszula Smolińska ◽  
Beata Kowalska ◽  
Magdalena Szczech ◽  
Anna Lisek ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Bacillus Amyloliquefaciens ◽  
Soil Microorganisms ◽  
Paenibacillus Polymyxa ◽  
Mineral Fertilizers ◽  
Bacterial Strains ◽  
Beneficial Effects ◽  
Purpureocillium Lilacinum ◽  
Bacteria And Fungi ◽  
Positive Effect

Abstract In recent years, the use of bio-fertilizers enriched with specially selected microorganisms has been used more and more often. The beneficial effects of bio-fertilizers enriched with consortia of microorganisms on strawberry plants have been reported previously. The purpose of the research was to determine the effect of bio-fertilizers containing selected fungal and bacterial strains on the microorganisms living in the rhizosphere of strawberry plants. In the experiments described in this paper, synthetic mineral fertilizers were enriched with selected microorganisms. The fertilizer urea was enriched with the fungi Aspergillus niger and Purpureocillium lilacinum, while the fertilizers Polifoska 6 and Super Fos Dar 40 with strains of the bacteria Bacillus sp., Bacillus amyloliquefaciens, and Paenibacillus polymyxa. Bacteria and fungi belonging to these species can exert a positive effect on the growth of many plants. The results obtained in this study showed that the application of fertilizers enriched with microorganisms had different effects on the analyzed populations of soil microorganisms in the rhizosphere of strawberry plants. There were evidences of both, an adverse effect of the applied fertilizer and/or microorganisms, but more often, the beneficial effect was found on the abundance of the microorganisms in the rhizosphere of the strawberry. The most effective for the population of Pseudomonas bacteria was application of urea and fungi and Polifoska and bacteria. The highest number of phosphorus utilizing bacteria B was scored in the treatments containing NPK, NPK + fungi and urea 60% + fungi. The application of NPK + fungi and urea 100% + fungi as well as Super Fos Dar with bacteria was most beneficial for population of actinomycetes.

scholarly journals The influence of diesel oil contamination on soil microorganisms and oat growth

2011 ◽  
Vol 48 (No. 2) ◽  
pp. 51-57
Author(s):  
J. Wyszkowska ◽  
J. Kucharski ◽  
E. Wałdowska
Keyword(s):  
Growth And Development ◽  
Soil Microorganisms ◽  
Diesel Oil ◽  
Positive Influence ◽  
Cellulolytic Bacteria ◽  
Negative Effect ◽  
Bacteria And Fungi ◽  
Positive Effect ◽  
Properties Of Soil ◽  
Microbiological Properties

The effect of diesel oil applied at 0, 2.4, 4.8 and 7.2 ml/kg of soil on yield of oat and number of oligotrophic, eutrophic, nitrogen immobilising, ammonifying and cellulolytic bacteria and Azotobacter sp., actinomyces and fungi was studied in a pot experiment. Inoculation with Streptomyces intermedius spores was used for soil detoxication. The experiment was performed in Eutric Cambisol soil derived from light clay sand. Diesel oil was found to have a negative effect on the growth and development of oat. Inoculation did not attenuate the response of oat to soil contamination with diesel oil, but it had a positive effect on oligotrophic and eutrophic bacteria as well as Azotobacter sp., nitrogen immobilising bacteria and fungi. Regardless of sown and unsown soil and inoculation with S. intermedius spores, diesel oil stimulated the number of oligotrophic, eutrophic, nitrogen immobilising bacteria and actinomyces. Sowing of oat positively affected microbiological properties of soil, because it had a positive influence on the relation of oligotrophic bacteria and actinomyces to fungi. This positive effect, however, was weakened by diesel oil.

scholarly journals Effect of Azotobacter chroococcum on sugar beet and microbial activity of rhizosphere

2010 ◽  
pp. 37-46
Author(s):  
Janja Kuzevski ◽  
Nada Milosevic ◽  
Sasa Krstanovic ◽  
Zora Jelicic
Keyword(s):  
Sugar Beet ◽  
Soil Microorganisms ◽  
Vegetation Period ◽  
Mineral Fertilizers ◽  
Atmospheric Nitrogen ◽  
Root Yield ◽  
Almost All ◽  
Biological Nitrogen ◽  
Number Of Bacteria ◽  
Soil Dehydrogenase Activity

In sugar beet production, one of the most important factors that affect the yield, apart from genetic properties, is the use of mineral fertilizers. Considerate amounts of mineral fertilizers are used in sugar beet production. However, if agroecological conditions are not optimum, mineral fertilizers cannot be completely absorbed, which may lead to soil contamination. Therefore, research has been focusing on ways of using atmospheric nitrogen by means of nitrogen-fixing bacteria. Numerous researches have proved that one part of mineral fertilizers can be replaced by biological nitrogen. The aim of this research was to determine the effect of genotype, azotobacter and the amount of mineral fertilizers on the root yield of sugar beet and on the microbiological activity of the sugar beet rhizospheric soil. Three hybrids of sugar beet were used during the two years of the research. The seed of the hybrids was inoculated with three strains of azotobacter. Various amounts of NPK were used (0;30;60;90 kg/ha). At the end of the vegetation period, the following were determined: root yield, total number of bacteria, number of azotobacter, oligotrophic bacteria, ammonifiers, fungi, and actinomycetes in soil. Dehydrogenase activity was measured. The results were processed statistically (analysis of variance for factorial trials) and the effect of the factors was determined upon the expected mean square values. The yield was mainly affected by the amount of mineral fertilizers. However, the effect of mineral fertilizers was different with different inoculation treatments. The effect of the examined factors was dependant upon genotype, amount of mineral fertilizers, inoculation and the year of trials. The interaction between genotype, mineral fertilizers, inoculation and the year of trials was the factor that had the greatest effect on the number of almost all the examined soil microorganisms.

Evidence of nitrogen fixation in lodgepole pine inoculated with diazotrophic Paenibacillus polymyxa

Botany ◽  
2012 ◽  
Vol 90 (9) ◽  
pp. 891-896 ◽  
Author(s):  
Amandeep Bal ◽  
Christopher P. Chanway
Keyword(s):  
Seedling Growth ◽  
Pinus Contorta ◽  
Lodgepole Pine ◽  
Growth Period ◽  
Paenibacillus Polymyxa ◽  
N Fixation ◽  
Bacterial Strains ◽  
Bacterial Inoculation ◽  
Foliar N ◽  
Negative Effect

Diazotrophic bacteria previously isolated from internal tissues of naturally regenerating lodgepole pine ( Pinus contorta var. latifolia (Dougl.) Engelm.) seedlings were tested for their ability to colonize and fix nitrogen (N) in pine germinants in two experiments. Surface sterilized pine seed was sown in glass tubes containing an autoclaved sand – montmorillonite clay mixture that contained a N-limited nutrient solution labeled with 15N as 0.35 mmol·L–1 Ca(15NO3)2 (5% 15N label). Pine seed was inoculated with one of three of the following bacterial strains: Paenibacillus polymyxa P2b-2R, P. polymyxa P18b-2R, or Dyadobacter fermentans P19a-2R, and seedlings grew for either 27 or 35 weeks. At the end of each plant growth period, P. polymyxa strain P2b-2R was detected in the pine rhizosphere but not inside plant tissues. Pine foliar N concentrations were not affected by bacterial inoculation but significant foliar 15N dilution was observed in seedlings treated with strain P2b-2R (30% and 66%, P < 0.05, in the first and second experiments, respectively). This strain also reduced seedling biomass in both experiments but effects were significant only in the second experiment (36%, P < 0.05). Notwithstanding the negative effect of bacterial inoculation on seedling growth, pine seedlings inoculated with strain P2b-2R derived 30% and 66%, respectively, of their foliar N from bacterial N fixation in two seedling growth experiments. These results demonstrate the possibility that some endophytic diazotrophs facilitate pine seedling growth in N-poor soils.

scholarly journals EFFECT OF BACILLUS GINSENGIHUMI M2.11 AND PANTOEA BRENNERI AS3 ON PLANT BIOMASS GROWTH AND SEED GERMINATION ENERGY

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 49-55
Author(s):  
D.L. Itkina ◽  
◽  
L.V. Sokolnikova ◽  
A.D. Suleimanova ◽  
M.R. Sharipova ◽  
...  
Keyword(s):  
Agricultural Development ◽  
Plant Biomass ◽  
Plant Protection ◽  
Average Length ◽  
Culture Fluid ◽  
Plant Protection Products ◽  
Practical Significance ◽  
Mineral Fertilizers ◽  
Bacterial Strains ◽  
Active Metabolites

In the modern conditions of agricultural development, the use of microbiological preparations as an alternative to mineral fertilizers and chemical plant protection products is gaining wide interest and practical significance. Soil microorganisms have the ability to interact with the root system of plants, optimize mineral nutrition, synthesize growth hormones and antimicrobial compounds that inhibit the development of phytopathogenic fungi and bacteria and have a stimulating effect on the growth and development of cultivated crops, increasing their resistance to phytopathogens and stress. The search for environmentally friendly technologies, the use of enzymes and active metabolites of bacterial origin, or the use of bacterial strains that promote plant growth is a promising direction. The effect of culture fluid of Bacillus ginsengihumi and Pantoea brenneri on the average length of the plant stem was studied. When processing wheat seeds of P. brenner AS3, the length of the first leaf increased by 50%, and B. ginsengihumi M2.11 by 25%.

scholarly journals Effect of the Sowing Method and Mineral Fertilizers on the Indicators of the Crop Yield Structure of Seed Alfalfa in the Ganja-Gazakh Zone of Azerbaijan

2021 ◽  
Vol 7 (7) ◽  
pp. 73-78
Author(s):  
P. Nabiyeva
Keyword(s):  
Crop Yield ◽  
Active Substance ◽  
Mineral Fertilizers ◽  
Mineral Fertilization ◽  
Yield Structure ◽  
Seed Crops ◽  
Positive Effect ◽  
Alfalfa Seeds

The article presents the results of research on the influence of the method of sowing and mineral fertilizers on the indicators of the structure of the yield of seed alfalfa in the Ganja-Gazakh zone of Azerbaijan. It has been established that the method of sowing and mineral fertilization has a positive effect on the indicators of the structure of the yield of seed alfalfa. For each method of sowing and mineral fertilization, alfalfa seeds had a positive effect on the indicators of the yield structure. To obtain irrigated gray-brown soils of high and stable yields of alfalfa seeds, it is necessary to form the density of the herbage of seed crops of 33.8–53.0 productive stems per 1 m of sowing methods 45 cm and the dose of mineral fertilizers N60P90K60 kg/ha active substance in the conditions of the Ganja-Gazakh zone Azerbaijan.

scholarly journals Pathogenetic Substantiation of the Therapeutic Impact on Microbiota in Irritable Bowel Syndrome

2019 ◽  
Vol 29 (4) ◽  
pp. 7-14
Author(s):  
Yu. O. Shulpekova ◽  
G. H. Babaeva ◽  
V. Yu. Rusyaev
Keyword(s):  
Irritable Bowel Syndrome ◽  
Intestinal Microbiota ◽  
Intestinal Bacteria ◽  
Host Cells ◽  
Intestinal Microbiome ◽  
Bacterial Strains ◽  
Blastocystis Hominis ◽  
Therapeutic Impact ◽  
Irritable Bowel ◽  
Positive Effect

Aim. This review aims to describe the nature of changes in the intestinal microbiota in irritable bowel syndrome (IBS) and provide a pathogenetic justification of the feasibility of a therapeutic impact on microbiota. General findings. An important aspect of the interaction of intestinal bacteria with the “host” cells is their contact with pattern recognition receptors of enterocytes, dendritic cell receptors, as well as a transcellular transport of antigens in the region of Peyer’s patches. The area of interaction of intestinal bacteria and the human body is not limited to the intestines. Intestinal bacteria demonstrate a significant humoral effect due to signalling molecules, some of which exhibit neurotransmitter properties. The study of the bacterial cross-feeding for various species, i.e. mutual use of nutrient substrates produced by bacteria of various species, is of a great interest. The development of a lowactivity inflammation in IBS can partly be explained by the increased interaction of flagellin with the corresponding receptor, as well as the influx of excess bacteria from the small intestine. The majority of studies on IBS have demonstrated the predominance of intestinal bacteria with pro-inflammatory potential (Enterobacteriaceae) and the lack of bacteria with a pronounced anti-inflammatory, antimicrobial and enzymatic action (Lactobacillus and Bifidobacterium), as well as increased mucus degradation. Similar changes are observed in inflammatory bowel diseases. Reduced microbial diversity increases susceptibility to intestinal infections and parasitoses, including those caused by protozoa conditionally pathogenic for adults, such as Blastocystis hominis hominis, Dientamoeba fragilis, Giardia lamblia. With the help of nutrition correction, the use of probiotics and functional foods containing certain probiotic strains, plant fibres (primarily psyllium) and, in some cases, nonabsorbable antibiotics, a positive effect can be achieved in a significant number of IBS patients. Recent works have shown that clinical improvement is accompanied by a change in the composition of the intestinal microbiota.Conclusion. For the pathogenetic treatment of irritable bowel syndrome, the use of non-drug treatment is justified, such as diet optimization and prescription of plant fibres and probiotic bacterial strains. The positive effect of such an approach is largely determined by modification of the intestinal microbiota composition. This opens up prospects for a further, more targeted impact on the intestinal microbiome.

scholarly journals The chemicals (formaldehyde and toluene) influence on soil microorganisms of leached chernozem

2016 ◽  
Vol 5 (3) ◽  
pp. 21-25
Author(s):  
Natalya Anatolyevna Ilyina ◽  
Tatyana Valentinovna Fufaeva ◽  
Natalya Anatolyevna Kazakova ◽  
Nataliya Mikhailovna Kasatkina ◽  
Evgeniya Alexandrovna Vilkova
Keyword(s):  
Soil Microorganisms ◽  
Heterotrophic Bacteria ◽  
Soil Formation ◽  
Chemical Production ◽  
Leached Chernozem ◽  
Physico Chemical ◽  
Production And Consumption ◽  
The Status ◽  
Number Of Microorganisms ◽  
Bacteria And Fungi

The paper assesses the status of the soil cover associated with the pollution of its waste chemical production and consumption. The authors present the data of formaldehyde and toluene influence on the abundance of actinomycetes, heterotrophic bacteria and fungi, as these groups of microorganisms provide self-purification capacity of the soil and participate in soil formation processes. In this paper microbiological and physico-chemical methods of research are used. The study of species composition changes of some soil microorganisms groups of leached chernozem under the influence of formaldehyde and toluene showed that this factor causes changes in the complex of soil microorganisms. This factor is reflected in the decreased species richness and diversity and increase of pollution-tolerant microorganisms. These studies investigate a number of microorganisms that provide self-purification capacity of the soil and participate in soil formation. The results show the nature of the influence of different doses of formaldehyde and toluene on the structure and functioning of the complex of soil microorganisms, as well as reveal the mechanism of action of chemicals (formaldehyde and toluene) on soil microbiota associated with its resistance and the manifestation of toxicity of the soil.

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