Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland

2011 ◽  
Vol 14 (4) ◽  
Author(s):  
A. Szczerba-Turek ◽  
J. Siemionek ◽  
A. Bancerz-Kisiel ◽  
A. Raś ◽  
W. Szweda
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Papillomavirus

scholarly journals Phylogenetic analysis of a partial L1 gene from bovine papillomavirus type 1 isolated from naturally occurring papilloma cases in the northwestern region of Turkey

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
M. Ozkan Timurkan ◽  
M. Eray Alcigir
Keyword(s):  
Phylogenetic Analysis ◽  
Histopathological Examination ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Sequencing Analysis ◽  
Molecular Characterisation ◽  
Northwestern Turkey ◽  
Northwestern Region

This study was aimed at the molecular characterisation of bovine papillomavirus type 1 (BPV-1) isolated from papilloma cases in the northwestern region of Turkey. BPV-1 is a widely occurring oncogenic virus in cattle and is associated with benign epithelial neoplasia which causes significant economic losses in dairy and beef cattle because of treatment costs. In this study, 29 suspected papilloma specimens were collected from cattle in northwestern Turkey. These samples underwent molecular characterisation via the polymerase chain reaction (PCR) and sequencing analysis as well as macroscopic and histopathological examination. The histopathological examinations confirmed papilloma as the main lesion type in the specimens. Of the 29 papilloma-like tissue samples that were collected, 11 (i.e. 37.93%) were detected as positive and determined as containing BPV-1 (11 of 11, 100%). Using a partial sequence for the L1 gene acquired from GenBank, phylogenetic analysis confirmed the presence of BPV-1 and revealed that the infection might have originated in cross bred domestic and imported cattle. This study provides potentially useful information on the origin and spread of this disease. Its results can potentially aid in the development of appropriate control measures and therapeutic or vaccination strategies against the BPV-1 strain of bovine papillomatosis.

scholarly journals Analysis of the long control region of bovine papillomavirus type 1 associated with sarcoids in equine hosts indicates multiple cross-species transmission events and phylogeographical structure

2014 ◽  
Vol 95 (12) ◽  
pp. 2748-2756 ◽  
Author(s):  
Hannah Trewby ◽  
Gizachew Ayele ◽  
Giuseppe Borzacchiello ◽  
Sabine Brandt ◽  
M. Saveria Campo ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Control Region ◽  
Long Control Region ◽  
Sequence Variant ◽  
South American ◽  
Bovine Papillomavirus ◽  
Dna Viruses ◽  
Species Association ◽  
Fitness Advantage ◽  
History Of

Papillomaviruses are a family of slowly evolving DNA viruses and their evolution is commonly linked to that of their host species. However, whilst bovine papillomavirus-1 (BPV-1) primarily causes warts in its natural host, the cow, it can also cause locally aggressive and invasive skin tumours in equids, known as sarcoids, and thus provides a rare contemporary example of cross-species transmission of a papillomavirus. Here, we describe the first phylogenetic analysis of BPV-1 in equine sarcoids to our knowledge, allowing us to explore the evolutionary history of BPV-1 and investigate its cross-species association with equids. A phylogenetic analysis of the BPV-1 transcriptional promoter region (the long control region or LCR) was conducted on 15 bovine and 116 equine samples from four continents. Incorporating previous estimates for evolutionary rates in papillomavirus implied that the genetic diversity in the LCR variants was ancient and predated domestication of both equids and cattle. The phylogeny demonstrated geographical segregation into an ancestral group (African, South American and Australian samples), and a more recently derived, largely European clade. Whilst our data are consistent with BPV-1 originating in cattle, we found evidence of multiple, probably relatively recent, cross-species transmission events into horses. We also demonstrated the high prevalence of one particular sequence variant (variant 20), and suggest this may indicate that this variant shows a fitness advantage in equids. Although strong host specificity remains the norm in papillomaviruses, our results demonstrate that exceptions to this rule exist and can become epidemiologically relevant.

scholarly journals Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
Jianjun Hu ◽  
Wanqi Zhang ◽  
Surinder Singh Chauhan ◽  
Changqing Shi ◽  
Yumeng Song ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dairy Cow ◽  
Complete Genome ◽  
Bovine Papillomavirus ◽  
Southern Xinjiang

scholarly journals Abortion storm of Yili Horses is Associated with Equus Caballus Papillomavirus 2 Variant Infection

2021 ◽  
Author(s):  
Panpan Tong ◽  
Ruli Duan ◽  
Xiaozhen Song ◽  
Nuerlan Palidan ◽  
Haifeng Deng ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Epidemiological Study ◽  
Causative Agent ◽  
Equus Caballus ◽  
Equine Arteritis Virus ◽  
Bovine Papillomavirus ◽  
Sequence Analyses ◽  
Equid Herpesvirus ◽  
Thoroughbred Horses ◽  
Nasal Swabs

Abstract Background: Nine different species of Equus caballus papillomavirus (EcPV) and three bovine papillomavirus (BPV) have been reported to infect horses, however, there are so far no describing such infections in China. In January 2021, an abortion storm occurred in Yili horses, as a result of which 50 out of 93 aborted fetus samples were found to be negative for equid herpesvirus (EHV) and equine arteritis virus (EAV).Results: In our pioneer study with Chinese horses, we first found EcPV-2 in the nasal swabs (4/230, 1.7%) of Yili horses, and semen (3/18, 16.7%) of the Thoroughbred horses. This indicated that EcPV can be indeed hosted by horses in China, and that EcPV-2 might be transmitted though breeding. Further detection of EcPVs in the lung tissues of aborted fetus in Yili horses, which were originally negative for equid herpesviruses, established that EcPV-2 was positive in 19 of 50 samples, thereby indicating that EcPV-2 might be a new pathogen causing of abortions. Thereafter, the sequence analyses for L1 genes sequences of 26 China’s EcPV-2 were performed which indicated that EcPV-2, that primarily infected the horses in China, shared 98.3%-99.9% nt identity with the already published sequences for EcPV-2. These observations indicated that EcPV-2 identified in the current study were highly similar variants of the previously identified strains of EcPV-2. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-2, found in the Chinese horses, was closely related to and clustered together with an already known EcPV-2a lineage. Conclusion: Our study provides the first evidence related to EcPV-2 infection in the Chinese horses, which can serve as a causative agent for Yili horse abortions, and thus can possibly lay the foundation for a systematic and detailed epidemiological study of this infection in the Chinese horses.

Quantitative PCR High-Resolution Melting (qPCR-HRM) curve analysis, a new approach to rapid detection and differentiation of bovine papillomavirus detected in equine sarcoids

2014 ◽  
Vol 17 (2) ◽  
pp. 239-245 ◽  
Author(s):  
A. Szczerba-Turek ◽  
A. Bancerz-Kisiel ◽  
K. Lipczyńska ◽  
J. Siemionek ◽  
A. Raś ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
High Resolution ◽  
Quantitative Pcr ◽  
Protein Identification ◽  
High Resolution Melting ◽  
Skin Lesions ◽  
Curve Analysis ◽  
Bovine Papillomavirus ◽  
Equine Sarcoid ◽  
Novel Variant

Abstract The aim of the study was to evaluate a novel diagnostic scheme which combines quantitative PCR and High-Resolution Melting (qPCR-HRM) curve analysis for rapid differentiation based on E5 partial CDS of bovine papillomavirus type 1 or 2 (BPV-1 or BPV-2), and to perform a phylogenetic analysis of the complete CDS of the E5 gene of BPV detected in equine sarcoids. Samples of 38 skin lesions obtained from 27 horses were collected for molecular examinations. All lesions were clinically diagnosed as sarcoids, but results of histopathological examinations did not always corroborate the clinical diagnosis. Although all the samples were positive for the presence of BPV DNA, after qPCR-HRM analysis 6 (16%) specimens were recognized as BPV-1 “wild”, 24 (63%) as BPV-1 “European” and 8 (21%) as a “variant” of BPV E5 ORF partial CDS. Phylogenetic analysis based on nucleotide sequences of E2 ORF partial CDS and E5 ORF complete CDS was conducted on 7 specimens, whose sequences were published in GenBank and recognized as: 2PL (Accession Number - Acc. No. KC684939) - “variant” BPV-1, 7aPL (Acc. No. KC684940) - “European” BPV-1, 10PL (Acc. No. KC693480) - “variant” BPV-1, 16PL (Acc. No. KC693484) - “variant” BPV-2, 17PL (Acc. No. KC693481) - “variant” BPV-1, 20aPL (Acc. No. KC693482) - “European” BPV-1 and 20cPL (Acc. No. KC693483) - “wild” BPV-1. Amino acid (aa) sequences of E5 ORF complete CDS were also analyzed. The E5 variant of aa sequences found in isolate 10PL (protein identification - ID: AGM 20700) is a novel variant of E5 ORF complete CDS of BPV-1 detected in equine sarcoid in Poland

scholarly journals Genomic and phylogenetic analysis of two novel bovine papillomaviruses, BPV-9 and BPV-10

2008 ◽  
Vol 89 (1) ◽  
pp. 158-163 ◽  
Author(s):  
Shinichi Hatama ◽  
Kiyoko Nobumoto ◽  
Toru Kanno
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Complete Genome Sequence ◽  
Major Capsid Protein ◽  
Bovine Papillomavirus ◽  
Genome Nucleotide Sequence ◽  
Consensus Motifs ◽  
Unassigned Genus ◽  
Sequence Similarities ◽  
Sequence Identities

Eight bovine papillomavirus (BPV) types, BPV-1–8, have been classified, based on genome nucleotide sequence similarities, in the genera Deltapapillomavirus (BPV-1 and -2), Epsilonpapillomavirus (BPV-5 and -8), Xipapillomavirus (BPV-3, -4 and -6) and an unassigned genus (BPV-7). We report here the complete genome sequence of two new BPV types isolated from separate epithelial squamous papilloma lesions on cattle teats. The genomes are 7303 and 7399 bp in length, respectively, and both have genetic organization and consensus motifs typical of papillomaviruses. A neighbour-joining phylogenetic tree revealed that both viruses cluster with BPV-3, -4 and -6. Nucleotide sequence identities of the BPV L1 major capsid protein of these two new BPVs with BPV-3, their closest relative, are 74.2 and 71.2 %, respectively. These results suggest that both viruses are new BPV types in the genus Xipapillomavirus, and they are designated BPV-9 and BPV-10.

scholarly journals Complete genome and phylogenetic analysis of bovine papillomavirus type 15 in Southern Xinjiang dairy cow

2020 ◽  
Author(s):  
Jianjun Hu ◽  
Wanqi Zhang ◽  
Surinder Singh Chauhan ◽  
Changqing Shi ◽  
Yumeng Song ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Complete Genome ◽  
Amplicon Sequencing ◽  
Bovine Papillomavirus ◽  
Skin Sample ◽  
Reading Frame ◽  
Neoplastic Lesion ◽  
Lxcxe Motif ◽  
L1 And L2 ◽  
Southern Xinjiang

AbstractIn this study, the complete genome sequence of bovine papillomavirus (BPV) type 15 (BPV Aks-02), a novel putative BPV type from a skin sample of a cow in southern Xinjiang, China was determined by collecting cutaneous neoplastic lesion, followed by DNA extraction and amplicon sequencing. The complete genome consisted of 7189 base pairs (G+C content of 42.50%) that encoded five early (E8, E7, E1, E2, E4) and two late (L1 and L2) genes. The E7 protein contained a consensus CX2CX29CX2C zinc-binding domain and an LxCxE motif. The nucleotide sequence of the L1 open reading frame (ORF) was related mostly (99%) to the L1 ORF of putative type BAPV-3 reference strain from GenBank. Phylogenetic analysis and sequence similarities based on the L1 ORF suggest that BPV type (BPV Aks-02) clustered with members of genus Xipapillomavirus as BPV15, and closely related to Xipapillomavirus 1.

scholarly journals Molecular and Phylogenetic Analysis of Bovine Papillomavirus Type 1: First Report in Iraqi Cattle

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mohammed A. Hamad ◽  
Ahmed M. Al-Shammari ◽  
Shoni M. Odisho ◽  
Nahi Y. Yaseen
Keyword(s):  
Phylogenetic Analysis ◽  
Histopathological Examination ◽  
Complete Sequence ◽  
Control Measures ◽  
Economic Losses ◽  
Bovine Papillomavirus ◽  
Sequencing Analysis ◽  
First Report ◽  
Malignant Lesions

This study aimed to provide the first molecular characterization of bovine papillomavirus type 1 (BPV-1) in Iraq. BPV is a widely spread oncogenic virus in Iraqi cattle and is associated with the formation of both benign and malignant lesions, resulting in notable economic losses in dairy and beef cattle. In the current study, 140 cutaneous papilloma specimens were collected from cattle in central Iraq. These samples were submitted to histopathological examination, PCR, and sequencing analysis. The histopathology revealed that the main lesion type among the specimens was fibropapilloma. BPV-1 DNA was detected in 121 of the samples (86.42%) in Iraqi cattle as the main causative agent for the disease. A partial sequence for the E2, L2 genes, and complete sequence for the E5 gene were deposited in GenBank. Phylogenetic analysis confirmed the presence of BPV-1 and showed that the origin of infection may be imported European cattle. Obtaining a complete E5 gene sequence enabled us to perform structural predictions. This study presents the first report of BPV-1 infection in the Iraqi cattle and contributes to extending the knowledge of the origin of the spread of this disease. The results of this study will aid in the development of appropriate control measures and therapeutic strategies.

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