scholarly journals Variasi Konsentrasi Glukosa pada Media Tumbuh dan Lama Fermentasi Dalam Memproduksi Etanol oleh Isolat BM1-CP14

2021 ◽  
Vol 9 (2) ◽  
pp. 208
Author(s):  
Nurul Lita Ajizah ◽  
I M. Mahaputra Wijaya ◽  
Nyoman Semadi Antara
Keyword(s):  
Glucose Concentration ◽  
Research Process ◽  
Total Dissolved Solids ◽  
Exponential Phase ◽  
Phase Curve ◽  
Growth Time ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Death Phase ◽  
Effect Of Glucose

This study was aimed determine the effect of glucose concentration on ethanol producing BM1-CP14 isolate media and to determine to optimum fermentation time to producing ethanol and to determine the growth phase curve of BM1-CP14 isolates. This study uses 4 glucose concentration (8, 12, 16 and 20%) and 3 fermentation time (14, 17 and 20 days). The experimental-explorative research process was carried out in several stages, namely rejuvenation of BM1-CP14 cultures, culture grow, cell isolates adjustment, glucose level on fermentation media measurement, fermentation, and distillation. The glucose concentration which are at the range of 10?18% have an optimum effect in producing ethanol The highest total ethanol was obtained from the glucose concentration of 16% with a fermentation time of 10 days having a difference in total dissolved solids of 8.30 (?% brix) resulting in the highest total ethanol of 38.75 mL. The glucose concentration of 8% resulted in a less than optimal total ethanol, which was 18.00 mL with a difference in total solids of 4.20 (?% brix), while the glucose concentration of 20% has a difference in total dissolved solids of 6.20 (?% brix) and produced a total ethanol of 26.50 mL. The Fermentation time of 14, 17, and 20 days was effected in producing ethanol, namely if the fermentation was longer, the total ethanol produces would be lower. The fermentation time of 14 days resulted in a total ethanol of 37.50 mL with a difference in total dissolved solids of 5.05 (?% brix). In longer fermentation of 17 and 20 days, the total ethanol produced was decreased, produced smaller total ethanol. The growth curve of BM1-CP14 isolates showed the growth isolates of BM1-CP14 with a growth time of 54 hours, starts from the exponential phase for 12 hours, the stationary phase for 18 hours and ends in the death phase. Keywords: Bacteria, BM1-CP14 isolate, ethanol, fermentation time, glucose concentration

scholarly journals The Production of Bioethanol from Rimau Gerga Lebong (Rgl) Orange Waste as an Alternative Energy

2018 ◽  
Vol 248 ◽  
pp. 04004
Author(s):  
Vike Darliyasi ◽  
Kurnia Herlina Dewi ◽  
Budiyanto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alternative Energy ◽  
Block Design ◽  
Trichoderma Viride ◽  
Total Dissolved Solids ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Ethanol Content ◽  
Significant Difference ◽  
Orange Waste

Bioethanol from Rimau Gerga Lebong (RGL) orange waste is one of the solution to overcome fuel oil problem. The aim of this research is to get the type of microorganisms and fermentation time that produce the highest ethanol from RGL orange waste. The research method used was Randomized Block Design (RBD) of two factors, namely type of microorganisms (Trichoderma viride, Saccharomyces cerevisiae, and Trichoderma viride + Saccharomyces cerevisiae) and fermentation time (3 days, 5 days, and 7 days. Within the three type of microorganisms with variations of fermentation time showed that the pH was able to carry out the fermentation process smoothly. The highest total dissolved solids were in the type of Trichoderma viride 3 days and 5 days, and the type of mix of microorganisms on the 3rd day. The highest ethanol content is in the type of Sachharomyces cerevisiae for 7 days. ANOVA result showed that the interaction between two treatments on the total dissolved solids experienced significant differences, so it continue with the DMRT test at a significant level of 0.5%. However, it is different from the results of ANOVA on ethanol content which showed that there were significant differences between ethanol content and types of microorganisms, but there was no significant difference on fermentation time

scholarly journals Pengaruh Penambahan Ragi Tape dan Waktu Fermentasi Hasil Samping Cairan Pulpa terhadap Karakteristik Mutu Cuka Kakao (Theobroma cacao L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 177
Author(s):  
Ida Bagus Gede Awidyanata ◽  
G.P Ganda Putra ◽  
Luh Putu Wrasiati
Keyword(s):  
Acetic Acid ◽  
Block Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Alcohol Content ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Natural Fermentation ◽  
Acid Ph ◽  
Cocoa Bean Fermentation

The aim of this study was to determine the effect of adding ragi tape and the time of natural fermentation of the pulp byproducts of the fermented cocoa beans on the characteristics of the quality of cocoa vinegar and determining the addition of tape yeast and the natural fermentation time of the best pulp fluid for cocoa vinegar production. The experiments in this study used a randomized block design with two factors. The first factor is the addition of yeast tape consisting of 5 levels, namely without the addition of yeast tape (control), the addition of yeast tape 0.05% (w / v), 0.10% (w / v), 0.15% (w / v), 0.20% (w / v), the second factor is the fermentation time consisting of 6 levels, namely 5, 10, 15, 20, 25, 30 days. Treatment The addition of tape yeast has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, has no significant effect on alcohol content in the fermented vinegar from the pulp fluid which is the by-product of cocoa bean fermentation. The length of fermentation treatment has a very significant effect on acetic acid, pH, total dissolved solids, total sugar, alcohol. The interaction between the two treatments had no significant effect on acetic acid, pH, total dissolved solids, alcohol content, and significant effect on total sugar in fermented cocoa vinegar products. The best treatment was obtained from the addition of 0.15% (w / v) yeast tape and 30 days fermentation time which produced cacao vinegar with acetic acid content of 2.40%, pH 3.37, total dissolved solids 4.25 obrix, total sugar 0.03%, and 0.00% alcohol. Keywords  : vinegar fermentasion, ragi tape, time of fermentation

scholarly journals Production of bioethanol from wild cassava crude starch (Manihot glaziovii Muell. Arg) using different microbial types and fermentation times

2021 ◽  
Vol 913 (1) ◽  
pp. 012032
Author(s):  
S V Mellicha ◽  
I B W Gunam ◽  
N S Antara ◽  
I W Arnata
Keyword(s):  
Ph Value ◽  
Block Design ◽  
Sugar Content ◽  
Dry Weight ◽  
Total Dissolved Solids ◽  
Reducing Sugar ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Cassava Plant ◽  
Manihot Glaziovii

Abstract Wild cassava (Manihot glaziovii Muell. Arg) is one of the varieties of cassava that contains toxic compounds cyanogenic glucosides, which cause unmarketable food products more suitable to be processed into bioethanol. This wild cassava plant produces tubers four times in weight compared to that of ordinary cassava, and the flesh contains about 40–70% starch by dry weight. This study aimed to determine the effect of the microbial types and fermentation times on bioethanol production from M. glaziovii Muell. Arg crude starch and to determine the type of microbes and fermentation time that can produce the highest bioethanol product from M. glaziovii Muell. Arg crude starch. This study consists of two factors using a factorial randomized block design (RBD). The first factor was the type of microbe which consists of 2 different microbes, namely R5I3 isolates and Saccharomyces cerevisiae ATCC 9763. The second factor was the fermentation time which consists of 4 levels, namely 3, 4, 5 and 6 days. The observed parameters were total dissolved solids value, pH value, reducing sugar content, and ethanol content. The data obtained were analyzed using analysis of variance (ANOVA) and continued with Tukey’s HSD post hoc tests. The results showed that fermentation using R5I3 isolates with a fermentation time of 5 days was the best treatment to obtain a maximum ethanol of 21.64±3.03 g/L, with a final pH value of 4.70±0.14, total dissolved solids 4.10±0.14°Brix and reducing sugar content of 0.91±0.02 g/L. Therefore, the new isolate R513 was highly potential for producing bioethanol from wild cassava crude starch.

scholarly journals Effect of Saccharomyces cerevisiae ATCC 9763 concentration and fermentation time on bioethanol content from corn stover crude cellulose substrate

2021 ◽  
Vol 913 (1) ◽  
pp. 012026
Author(s):  
A M M Napitupulu ◽  
L Suhendra ◽  
I B W Gunam
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Corn Stover ◽  
Ph Value ◽  
Block Design ◽  
Sugar Content ◽  
Total Dissolved Solids ◽  
Reducing Sugar ◽  
Dissolved Solids ◽  
Fermentation Time ◽  
Ethanol Content

Abstract Corn stover is a waste from the corn plant that dried in the fields after the corn cobs were harvested. From many corn wastes produced, there is very little utilization to corn stover. Delignified corn stover contains 65.46% cellulose, 14.58% hemicellulose, and 8.66% lignin. Lignocellulosic biomass is very difficult to biotransform, therefore it must be delignified to break the bonds between cellulose, hemicellulose, and lignin. Then the cellulose is converted into sugars by saccharification using crude cellulose enzymes so it can be converted into bioethanol through a fermentation process using simultaneous saccharification and fermentation (SSF) method. This study aims to determine the concentration of Saccharomyces cerevisiae ATCC 9763 and the optimum fermentation time in order to obtain high content of bioethanol from corn stover. Bioethanol production at different concentrations of S. cerevisiae and fermentation time uses a factorial randomized block design (RBD) consisting of two factors. The first factor was the concentration of S. cerevisiae which consisted of 3 levels, namely 3%, 5%, and 7% (v/v). The second factor was the fermentation time which consists of 4 levels, namely 24 hours, 48 hours, 72 hours, and 96 hours. Observed variables included pH value, total dissolved solids, reducing sugar content, and ethanol content. The data obtained were analyzed for its diversity using analysis of variants (ANOVA) and continued with the HSD Tukey. The results showed that the concentration of S. cerevisiae 7% (v/v) and a fermentation time of 96 hours was the best treatment to obtain a maximum ethanol content of 7.53 ± 0.330 g/L, with a final pH value of 4.25 ± 0.07, total dissolved solids 2.9 ± 0.14 °Bx and reducing sugar content of 0.334 ± 0.03 g/L. Increasing the concentration of S. cerevisiae and fermentation time can increase the ethanol content.

PHYSICO-CHEMICAL ASPECTS OF YAMUNA RIVER AT GOKUL BARRAGE, MATHURA (UP) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
AJAY KUMAR RAJAWAT ◽  
PRAVEEN KUMAR
Keyword(s):  
Water Samples ◽  
Suspended Solids ◽  
Total Dissolved Solids ◽  
Tolerance Limit ◽  
Chemical Condition ◽  
Yamuna River ◽  
Dissolved Solids ◽  
Time Period ◽  
Physico Chemical ◽  
Almost All

An attempt has been made to study the Physico-chemical condition of water of Yamuna River at Gokul Barrage, Mathura, (UP). The time period of study was July 2015 to June 2016. Three water samples were selected from different sites in each month for study. The parameters studied were Temperature, Turbidity, pH, DO, BOD, COD, Total Dissolved Solids and Suspended Solids. Almost all the parameters were found above the tolerance limit.

Performance Study of Some Reverse Osmosis Systems for Removal of Uranium and Total Dissolved Solids in Underground Waters of Punjab State, India

2014 ◽  
Vol 4 (2) ◽  
pp. 467-476
Author(s):  
Nisha Sharma ◽  
Jaspal Singh ◽  
Barjinder Kaur
Keyword(s):  
Drinking Water ◽  
Reverse Osmosis ◽  
State Government ◽  
Total Dissolved Solids ◽  
Internal Exposure ◽  
Drinking Water Source ◽  
Performance Study ◽  
Dissolved Solids ◽  
Underground Waters ◽  
High Concentrations

Radionuclides (uranium, thorium, radium, radon gas etc.) are found naturally in air, water, soil and rock. Everyday, we ingest and inhale these radionuclides through the air we breathe and through food and water we take. Out of the internal exposure via ingestion of radionuclides, water contributes the major portion. The natural radioactivity of water is due to the activity transfer from bed rock and soils. In our surveys carried out in the past few years, we have observed high concentrations of uranium and total dissolved solids (TDS) in drinking waters of some southern parts of Punjab State exceeding the safe limits recommended by national and international agencies. The main drinking water source is the underground water procured from different depths. Due to the highly saline taste, disorders in their digestive systems and other ailments, people are installing reverse osmosis (RO) systems in their houses. Some RO systems have been installed on commercial basis. The state government is also in the process of installing community RO systems at the village level. As high values of uranium are also undesired and may pose health hazards due to radioactivity and toxicity of uranium, we have conducted a survey in the field to study the performance of various RO systems for removal of uranium and TDS. Water samples from about forty RO systems from Faridkot, Mansa, Bathinda and Amritsar districts of Punjab State were collected and analyzed. Our results show that some RO systems are able to remove more than 99% of uranium in the underground waters used for drinking purposes. TDS values are also reduced considerably to the desired levels. So RO systems can be used to avoid the risk of unduly health problems posed by high concentrations of uranium and TDS in drinking water.

PENGARUH KONSENTRASI RAGI Saccharomyces cerevisiae DAN LAMA FERMENTASI TERHADAP KUALITAS CUKA TOMI-TOMI (Flacourtia inermis)

2015 ◽  
Vol 4 (2) ◽  
pp. 50-55
Author(s):  
Sandra J Nendissa ◽  
Rachel Breemer ◽  
Nikholaus Melamas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Design ◽  
Total Dissolved Solids ◽  
Total Sugar ◽  
Dissolved Solids ◽  
Randomized Experimental Design ◽  
Two Factors ◽  
Four Levels

This objectives of this research were both to study and determine the best level of concentration of yeast Saccharomyces cereviseae and period of fermentation on the quality of tomi-tomi vinegar (Flacourtia inermis). A completely randomized experimental design with two factors of treatment was applied in this research. The first factor was concentration of yeast S. cereviseae having four levels of tretament, i.e.: without the addition of yeast 0.5, 1 and 1.5 g yeast. The second factor was period fermentation with 1, 2, 3, 4, and 5 weeks. The result indicated that the concentration of yeast S. cereviseae 1.5 g and period fermentation 5 week produced a good tomi-tomi vinegar with total acids 51.22%, total dissolved solids 8.35, total sugar 8.07% and pH 5.40.

Combination of Carbonated Aloe Vera Drink with Lemon Juice

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Jefrianta Demu Geri ◽  
Dewi Fortuna Ayu ◽  
Noviar Harun
Keyword(s):  
Vitamin C ◽  
Aloe Vera ◽  
Sweet Taste ◽  
Total Dissolved Solids ◽  
Lemon Juice ◽  
Dissolved Solids ◽  
Combination Treatments ◽  
Randomized Design ◽  
Yellow Color ◽  
Range Test

The purpose of this study was to determine the optimal ratio of carbonated aloe vera drink and lemon juice. This research used completely randomized design with four treatments and four replications. The treatments performed were L1 (90% carbonated aloe vera and 10% lemon juice), L2 (80% carbonated aloe vera and 20% lemon juice), L3 (70% carbonated aloe vera and 30% lemon juice), and L4 (60% carbonated aloe vera and 40% lemon juice). Data were statistically analyzed by using analysis of variance (ANOVA) and followed by duncan new multiple range test (DNMRT) at level 5%. The combination treatments of carbonated aloe vera drinks and lemon juice had a significant effect on acidity (pH), vitamin C, total dissolved solids, and descriptive and hedonic sensory assessment. The best treatment from the research was L3 (70% carbonated aloe vera and 30% lemon juice) with pH of 4.26, vitamin C of 19.97 mg, and total dissolved solids of 14.34°brix. The overall sensory test result preferred by panelists with descriptions of yellow color, lemon flavor, and had a slightly sweet taste.

Study and Evaluation of Stored Drinking Water Characteristics Using Three Types of Roof Water Tanks in Kirkuk Governorate, Iraq

2019 ◽  
Vol 7 (1) ◽  
pp. 11-16
Author(s):  
Abdulkhaleq K Mahmood ◽  
Ali A Kamal ◽  
Ako R Hama
Keyword(s):  
Drinking Water ◽  
Storage Time ◽  
Total Dissolved Solids ◽  
Water Tank ◽  
Dissolved Solids ◽  
Water Characteristics ◽  
Three Samples ◽  
Water Metal ◽  
Water Tanks ◽  
Better Than

The scarcity of safe drinking water is one of the problems faced by the majority of cities in the world. Kirkuk city is one of these cities, which suffer from a shortage of drinking water. People have adopted the use of different rooftop tanks to overcome this problem. This research focuses on studying the effect of storage time on the five main characteristics of drinking water, which include, acid index (pH), electrical conductivity (EC), total suspended solids (TSS), total dissolved solids (TDS), and turbidity (Tr). Three types of tanks were used predominantly (galvanized metal, plastic, and aluminum tanks). By analyzing the results, the characteristics of three samples of municipal source water obtained. Three samples were taken from each tank at different periods (4, 8, and 12 days). The results showed that the storage time affected the characteristics of drinking water. These characteristics differed from one tank to another. Metal tanks showed an increase in total dissolved solids, due to the evaporation process, even as plastic and aluminum tanks showed an increase in pH. The properties of all storage water tanks changed with times, but overall, the results were within the Iraqi limitation for drinking water. It was not easy to only depend on the results of this study to believe that any one type of water tank was better than the other, as the values of most of the variables studied had varied from one type to other. However, many studies have indicated a number of health risks, and most significantly with regard to plastic tanks, which are said to contain dangerous organic compounds that can be transferred to water. Metal tanks can cause zinc leakage, caused by a number of environmental factors at high levels. Aluminum tanks also can have an effect on the water in tanks.

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