scholarly journals Kedilerde feline coronavirus (FCoV) enfeksiyonunun kalsiyum metabolizması üzerindeki etkisinin araştırılması

2021 ◽  
Author(s):  
Hasan Barış CENGİZ ◽  
Halil İbrahim GÖKÇE
Keyword(s):  
Feline Coronavirus

scholarly journals High-throughput viral microneutralization method for feline coronavirus using image cytometry

2020 ◽  
Vol 286 ◽  
pp. 113979
Author(s):  
Morgan Pearson ◽  
Alora LaVoy ◽  
Leo Li-Ying Chan ◽  
Gregg A. Dean
Keyword(s):  
High Throughput ◽  
Image Cytometry ◽  
Feline Coronavirus

scholarly journals Subarachnoid diverticulum associated with feline infectious peritonitis in a Siberian cat

2020 ◽  
Vol 6 (2) ◽  
pp. 205511692094147
Author(s):  
Christopher Hoey ◽  
George Nye ◽  
Angela Fadda ◽  
Janet Bradshaw ◽  
Emi N Barker
Keyword(s):  
Cervical Spinal Cord ◽  
Serum Biochemistry ◽  
Acute Onset ◽  
Neurological Deficits ◽  
Rt Pcr ◽  
Feline Infectious Peritonitis ◽  
Case Summary ◽  
Choroid Plexitis ◽  
The Brain ◽  
Feline Coronavirus

Case summary A 7-month-old Siberian cat was presented for investigation of acute onset multifocal neurological deficits. Neurological examination documented dull mental status and an ambulatory left hemiparesis. Serum biochemistry documented marked hyperglobulinaemia. MRI of the brain identified marked leptomeningeal contrast enhancement extending along the brainstem caudally to involve the cranial cervical spinal cord. MRI of the cervical spine further identified a subarachnoid diverticulum that extended from the level of the obex to the C2–C3 vertebrae. Cerebrospinal fluid quantitative RT-PCR was positive for the presence of feline coronavirus. Histopathology revealed pyogranulomatous meningitis and choroid plexitis, uveitis and nephritis. Relevance and novel information This article describes the first reported case of a subarachnoid diverticulum associated with feline infectious peritonitis.

scholarly journals Effect of cat litters on feline coronavirus infection of cell culture and cats

2019 ◽  
Vol 22 (4) ◽  
pp. 350-357 ◽  
Author(s):  
Diane Addie ◽  
Lene Houe ◽  
Kirsty Maitland ◽  
Giuseppe Passantino ◽  
Nicola Decaro
Keyword(s):  
Cell Culture ◽  
Virus Infection ◽  
Real Life ◽  
In Vitro Study ◽  
Oral Route ◽  
Virus Shedding ◽  
Virus Load ◽  
Fuller’S Earth ◽  
Feline Coronavirus

Objectives Feline infectious peritonitis (FIP) is caused by infection with feline coronavirus (FCoV). FCoV is incredibly contagious and transmission is via the faecal–oral route. FCoV infection, and therefore FIP, is most common in breeder and rescue catteries, where many cats are kept indoors, using litter trays. Whether it is possible to break the cycle of FCoV infection and reinfection using cat litters has never been investigated. The aim of the study was to examine the effect of cat litters on FCoV infectivity and virus load in multi-cat households, and transmission frequency. Methods Fifteen cat litters were mixed and incubated with FCoV, centrifuged and the supernatants tested in vitro for the ability to prevent virus infection of cell culture. To test applicability of in vitro results to real life, virus load was measured in two households in a double crossover study of four Fuller’s earth-based cat litters by testing rectal swabs using FCoV reverse transcriptase quantitative PCR. Results Four litters abrogated FCoV infection of cell culture, nine reduced it to a greater or lesser extent and two had no effect. One brand had different virus inhibitory properties depending on where it was manufactured. Fuller’s earth-based litters performed best, presumably by adsorbing virus. In the field study, there appeared to be less virus shedding on one Fuller’s earth-based cat litter. Conclusions and relevance The in vitro study successfully identified cat litters that inactivate FCoV; such litters exist so do not need to be developed. Fuller’s earth-based litters best prevented infection of cell culture, but did not completely abrogate FCoV transmission in two multi-cat households. A dust-free clumping Fuller’s earth litter appeared to fare best, but virus shedding also varied on the control litters, complicating interpretation. Sawdust-based cat litters are not useful in FCoV-endemic households because they track badly and have a poor effect on virus infection.

scholarly journals In Vitro Effects of Doxycycline on Replication of Feline Coronavirus

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 312
Author(s):  
Magdalena Dunowska ◽  
Sayani Ghosh
Keyword(s):  
Inhibitory Concentration ◽  
Infectious Virus ◽  
Treatment Options ◽  
Inhibitory Effect ◽  
Virus Titration ◽  
Reverse Transcription Quantitative Pcr ◽  
In Vitro Effects ◽  
Dose Dependent ◽  
Feline Coronavirus

Feline infectious peritonitis (FIP) is a sporadic fatal disease of cats caused by a virulent variant of feline coronavirus (FCoV), referred to as FIP virus (FIPV). Treatment options are limited, and most of the affected cats die or are euthanized. Anecdotally, doxycycline has been used to treat FIP-affected cats, but there are currently no data to support or discourage such treatment. The aim of this study was to establish whether doxycycline inhibits replication of FIPV in vitro. The virus was cultured in Crandell-Rees feline kidney cells with various concentrations of doxycycline (0 to 50 µg/mL). The level of FIPV in cultures was determined by virus titration and FCoV-specific reverse-transcription quantitative PCR. Cell viability was also monitored. There was no difference in the level of infectious virus or viral RNA between doxycycline-treated and untreated cultures at 3, 12- and 18-hours post-infection. However, at 24 h, the growth of FIPV was inhibited by approximately two logs in cultures with >10 µg/mL doxycycline. This inhibition was dose-dependent, with inhibitory concentration 50% (IC50) 4.1 µg/mL and IC90 5.4 µg/mL. Our data suggest that doxycycline has some inhibitory effect on FIPV replication in vitro, which supports future clinical trials of its use for the treatment of FIP-affected cats.

scholarly journals Development of Antiviral CVC (Chief Value Cotton) Fabric

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2601
Author(s):  
Wen-Yi Wang ◽  
Sui-Lung Yim ◽  
Chun-Ho Wong ◽  
Chi-Wai Kan
Keyword(s):  
High Risk ◽  
Antiviral Activity ◽  
Cotton Fabric ◽  
Protective Clothing ◽  
Feline Coronavirus

The outbreak of COVID-19 has already generated a huge societal, economic and political losses worldwide. The present study aims to investigate the antiviral activity of Poly(hexamethylene biguanide) hydrochloride (PHMB) treated fabric against COVID-19 by using the surrogate Feline coronavirus. The antiviral analysis indicated that up to 94% of coronavirus was killed after contacting the CVC fabric treated with PHMB for 2 h, which suggests that PHMB treated fabric could be used for developing protective clothing and beddings with antiviral activity against coronavirus and can play a role in fighting the transmission of COVID-19 in the high-risk places.

scholarly journals The Population Diversity of Candidate Genes for Resistance/Susceptibility to Coronavirus Infection in Domestic Cats: An Inter-Breed Comparison

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 778
Author(s):  
Jana Bubenikova ◽  
Leona Vychodilova ◽  
Karla Stejskalova ◽  
Jan Futas ◽  
Jan Oppelt ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Single Gene ◽  
Differential Susceptibility ◽  
Snp Markers ◽  
Population Diversity ◽  
Population Substructure ◽  
Domestic Cats ◽  
Coronavirus Infection ◽  
Breed Comparison ◽  
Feline Coronavirus

Feline coronavirus (FCoV) is a complex pathogen causing feline infectious peritonitis (FIP). Host genetics represents a factor contributing to the pathogenesis of the disease. Differential susceptibility of various breeds to FIP was reported with controversial results. The objective of this study was to compare the genetic diversity of different breeds on a panel of candidate genes potentially affecting FCoV infection. One hundred thirteen cats of six breeds were genotyped on a panel of sixteen candidate genes. SNP allelic/haplotype frequencies were calculated; pairwise FST and molecular variance analyses were performed. Principal coordinate (PCoA) and STRUCTURE analyses were used to infer population structure. Interbreed differences in allele frequencies were observed. PCoA analysis performed for all genes of the panel indicated no population substructure. In contrast to the full marker set, PCoA of SNP markers associated with FCoV shedding (NCR1 and SLX4IP) showed three clusters containing only alleles associated with susceptibility to FCoV shedding, homozygotes and heterozygotes for the susceptibility alleles, and all three genotypes, respectively. Each cluster contained cats of multiple breeds. Three clusters of haplotypes were identified by PCoA, two clusters by STRUCTURE. Haplotypes of a single gene (SNX5) differed significantly between the PCoA clusters.

scholarly journals Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b

2016 ◽  
Vol 184 ◽  
pp. 11-19 ◽  
Author(s):  
Tanja Lemmermeyer ◽  
Benjamin Lamp ◽  
Rainer Schneider ◽  
John Ziebuhr ◽  
Gergely Tekes ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Accessory Protein ◽  
Feline Coronavirus

scholarly journals Ultrastructure of Felis catus whole fetus (Fcwf-4) cell culture following infection with feline coronavirus

2011 ◽  
Vol 60 (4) ◽  
pp. 275-282 ◽  
Author(s):  
A. Alazawy ◽  
S.-S. Arshad ◽  
M.-H. Bejo ◽  
A.-R. Omar ◽  
T.-A. Tengku Ibrahim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Felis Catus ◽  
Feline Coronavirus
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