scholarly journals Efecto del extracto de plantas medicinales sobre el crecimiento de Escherichia coli y Staphylococcus aureus

2021 ◽  
Vol 3 (3) ◽  
pp. 31
Author(s):  
Flor Teresa García Huamán ◽  
Marleny Angeles Trauco
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Taraxacum Officinale ◽  
Plantago Major ◽  
Matricaria Chamomilla ◽  
Dodonaea Viscosa ◽  
Bidens Pilosa ◽  
E Coli

<p>El objetivo de este estudio fue determinar el efecto del extracto de plantas medicinales sobre el crecimiento de Escherichia coli y Staphylococcus aureus crecimiento de <em>Escherichia coli </em>y <em>Staphylococcus aureus. </em>El diseño de estudio fue experimental, diseño factorial, de corte transversal. Se aislaron las bacterias y se obtuvieron extractos, utilizando 1 L. de etanol (QP) por cada 100g. de hoja triturada. Se almacenó a temperatura ambiente por 20 días, se filtró y evaporó a 40°C. Posteriormente se colocó 10ul a discos de papel de 6mm., luego se realizó la siembra y colocación de los discos, después de 48 horas se midió los halos de inhibición. Se encontró que los extractos no afectan el crecimiento de <em>E. Coli </em>pero si inhiben el crecimiento de <em>Staphylococcus aureus. </em>Se concluye que el efecto de los extractos de las plantas medicinales como: <em>Aloysia citriodora </em>“cedrón”, <em>Ambrosia arborescens </em>“marco”, <em>Bidens pilosa </em>“cadillo”, <em>Dodonaea viscosa </em>“chamana”, <em>Matricaria chamomilla </em>“manzanilla, <em>Minthostachys mollis </em>“poleo”, <em>Psidium guajaba </em>“guayaba”, <em>Plantago major </em>“llantén”, <em>Taraxacum officinale </em>“ diente de león” es negativa para <em>Escherichia coli</em>, pues no se evidenciaron halos de inhibición a diferencia de <em>Staphylcoccus aureus </em>donde se observó un efecto positivo, evidenciándose halos de inhibición, siendo el mayor (23.3 mm) con <em>Aloysia citridora </em>y el menor (9 mm) con <em>Matricaria chamonilla.</em></p>

scholarly journals Estudio fitoquímico y actividad antimicrobiana de plantas medicinales empleadas para el control de infecciones urinarias

2021 ◽  
Vol 16 (1) ◽  
pp. 43-56
Author(s):  
Lida Vanessa Hernández Moreno ◽  
Ludy Cristina Pabón Baquero ◽  
Patricia Hernández-Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Zingiber Officinale ◽  
Taraxacum Officinale ◽  
Portulaca Oleracea ◽  
Uncaria Tomentosa ◽  
E Coli ◽  
Anthoxanthum Odoratum ◽  
Parietaria Officinalis

Las enfermedades infecciosas han sido un desafío para la humanidad. A pesar de los avances en la ciencia, aún muchas infecciones no tienen tratamientos efectivos o los microorganismos han generado resistencia a los antibióticos, de manera que las plantas medicinales son una alternativa de tratamiento y reducción de la resistencia. El objetivo de este estudio fue realizar un análisis fitoquímico preliminar y evaluar la actividad antimicrobiana de doce extractos etanólicos deplantas empleadas en la medicina tradicional colombiana como control de infecciones urinarias (IU). Las especies Anthoxanthum odoratum (grama), Urera caracasana (ortigón), Equisetum bogotenses (cola de caballo), Parietaria officinalis (parietaria), Achyrocline bogotensis (vira vira), Kohleria hirsuta (caracola), Taraxacum officinale (diente de león), Sedum praealtum (siempre viva), Portulaca oleracea (verdolaga), Petroselinum sativum (perejil), Zingiber officinale (jengibre) y Uncaria tomentosa (uña de gato) fueron adquiridas en la plaza de mercado Soacha (Cundinamarca) y sometidas a maceración con etanol a temperatura ambiente. La actividad antimicrobiana se evaluó por difusión en agar, microdilución en placa y bioautografía frente a Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923) y Pseudomonas aeruginosa (ATCC 9027). Se determino que S. aureus y P. aeruginosa presentaron inhibición frente a cinco extractos en los tres métodos realizados, mientras que E. coli no fue inhibida por ningún extracto. Se estableció mediante bioautografía con reveladores específicos que los esteroides y/o triterpenoides, fenoles y flavonoides son los posibles metabolitos responsables de la actividad. Este tipo de investigaciones propicia estudios orientados al aislamiento de principios activos con utilidad en la elaboración de medicamentos para tratar IU.

scholarly journals Estudo da ação antimicrobiana conjunta de extratos aquosos de Tansagem (Plantago major l., Plantaginaceae) e Romã (Punica granatum l., Punicaceae) e interferência dos mesmos na ação da amoxicilina in vitro

2014 ◽  
Vol 16 (2 suppl 1) ◽  
pp. 323-328 ◽  
Author(s):  
D.G. Teles ◽  
M.M. Costa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Punica Granatum ◽  
Plantago Major ◽  
E Coli ◽  
Para Determinar ◽  
Punica Granatum L

O uso de plantas medicinais no tratamento de doenças (fitoterapia) é uma prática de diversos povos, sendo realizada desde a Antiguidade. Diversos estudos são realizados para determinar a eficácia dessas plantas, sendo desconhecida a existência de interações e interferências na combinação das mesmas, quer seja benéfica, quer cause algum dano para o organismo. A fim de descobrir a existência dessas interações ou a interferência na ação do antibiótico amoxicilina testou-se Punica granatum e Plantago major contra as bactérias Staphylococcus aureus e Escherichia coli. A matéria prima foi obtida nas regiões de Montes Claros e Curvelo e seus extratos aquosos foi obtido através da decocção. A Concentração Inibitória Mínima (CIM) dos extratos foi determinada pela técnica de microdiluição em caldo. Para o teste de interferência realizou-se o teste de disco-difusão. No teste de microdiluição em caldo, Punica granatum apresentou CIM de 6,25% contra o S. aureus e de 25% contra E. coli. O extrato de Plantago major não apresentou atividade contra as bactérias. O teste de disco-difusão confirmou a existência de interações, sendo apenas quatro resultados significativos (Amoxicilina + Plantago major Pura (PgP), Amoxicilina + Plantago major Diluição 1 (PgD1) e Amoxicilia + Plantago major Diluição 2 (PgD2) contra E. coli e Amoxicilina + PgP contra S. aureus). Conclui-se que existe interação dos extratos entre si e dos extratos com a amoxicilina, o que também pode acontecer com outros antibióticos e gerar riscos para a população. Portanto é indispensável à realização de testes que complementem essa pesquisa oferecendo mais informação à população para segurança na utilização de plantas medicinais. informações à população assegurando sua segurança na utilização de plantas medicinais.

scholarly journals Avaliação da atividade antibacteriana de extratos etanólico e de ciclohexano a partir das flores de camomila (Matricaria chamomilla L.)

2014 ◽  
Vol 16 (3) ◽  
pp. 521-526 ◽  
Author(s):  
A.F. Carvalho ◽  
D.M. Silva ◽  
T.R.C Silva ◽  
E. Scarcelli ◽  
M.R. Manhani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Salmonella Enterica ◽  
Matricaria Chamomilla ◽  
E Coli

Devido à crescente seleção de microrganismos resistentes aos antimicrobianos atuais, tem-se valorizado a busca por alternativas naturais. O presente estudo teve por objetivo avaliar a atividade antibacteriana de extratos etanólico e de ciclohexano de flores de camomila, espécie vegetal de uso antigo pela medicina tradicional, frente às bactérias ATCC Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli e Salmonella enterica subsp. enterica sorovar Typhimurium pelas técnicas de difusão em ágar e diluição em caldo. Foi observada inibição do crescimento de P. aeruginosa frente ao extrato etanólico bruto (1g/mL) na técnica de diluição em caldo, e confirmada pela técnica de difusão em ágar (halo de inibição de 10 mm de diâmetro). Para as demais bactérias testadas, os extratos e suas diluições não apresentaram efeito bacteriostático em nenhuma das técnicas. Pode-se concluir que o extrato etanólico bruto da camomila apresentou atividade antibacteriana frente à P. aeruginosa, porém não foi eficaz frente à S. aureus, E. coli e Salmonella enterica subsp. enterica sorovar Typhimurium. Portanto, são necessários novos estudos com diferentes linhagens de microrganismos, com o intuito de corroborar e assegurar os resultados apresentados, para definir o potencial antimicrobiano do extrato da camomila.

Antimicrobial Activity of Bidens pilosa Leaves Extracts Against Staphylococcus aureus and Escherichia coli

2021 ◽  
Vol 38 ◽  
pp. 20-24
Author(s):  
S.A. Mohamed ◽  
C. Mathew
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Bidens Pilosa ◽  
Leaf Extracts ◽  
E Coli ◽  
Tract Infections

Resistance against synthetic antimicrobial agents is one of the major global public health challenges that compel scientists to search for alternatives including those of plants origin. Staphylococcus aureus and Escherichia coli are bacteria responsible for a variety of infections and diseases that causes significant morbidity and mortality in humans and animals. E. coli is widely distributed in nature and commonly found in lower gastro intestinal tracts of most warm-blooded animals associated with urinary tract infections and enterocolitis in humans and colibacillosis in poultry. This study was carried out to investigate antimicrobial activity of methanolic leaf extracts of Bidens pilosa against S. aureus and E. coli. Agar well diffusion method was used to assess antimicrobial activity of the leaf extracts at 20%, 50% and 70% concentrations respectively based on measured zone of inhibition. The leaf extracts of Bidens pilosa produced significant zone of inhibition indicating its antimicrobial activity against E. coli and S. aureus. The antimicrobial activity was demonstrated in all concentrations however, the highest zone of inhibition (18.5mm and 32mm) for E. coli and S. aureus respectively was at 70% concentration. The results shows that Bidens pilosa leaf extracts have antimicrobial activity against the tested bacteria and have the potential for further development including identification of active components that can be tested for treatment of E. coli and S. aureus associated conditions.

EFECTO INHIBITORIO DE EXTRACTOS DE Vitex mollis Kunth CONTRA BACTERIAS Y ESPECIES DE Fusarium DE IMPORTANCIA HUMANA Y AGRÍCOLA

2018 ◽  
Vol 41 (4) ◽  
pp. 353-363
Author(s):  
Alberto J. Valencia-Botin ◽  
Melesio Gutiérrez-Lomelí ◽  
Juan A. Morales-Del-Río ◽  
Pedro J. Guerrero-Medina ◽  
Miguel A. Robles-García ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Enterica ◽  
Micrococcus Luteus ◽  
E Coli

Actualmente existe la necesidad de hacer frente al problema de la resistencia a los antibióticos y al uso indiscriminado de fungicidas químicos en la agricultura. El objetivo de este trabajo fue evaluar el efecto inhibitorio de extractos acuosos, metanólicos, acetónicos y hexánicos de hoja y tallo de Vitex mollis Kunth (Lamiaceae) contra diferentes bacterias (Escherichia coli, Micrococcus luteus, Salmonella enterica y Staphylococcus aureus) y especies del hongo Fusarium (F. verticillioides, F. oxysporum, F. tapsinum y F. oxysporum f.sp. lycopersici) de importancia en la salud y en la agricultura, así como determinar su composición química general. Se determinaron las concentraciones inhibitorias mínimas (CIM) de todos los extractos por la técnica de microdilución, excepto del hexánico, que no presentó inhibición en las bacterias estudiadas. S. enterica fue la bacteria que mostró mayor sensibilidad al extracto metanólico de tallo (CIM = 28 μg mL-1), le siguieron M. luteus (CIM = 32 μg mL-1), S. aureus (CIM = 75 μg mL-1) y E. coli (CIM = 80 μg mL- 1). Los extractos metanólicos y acuosos de tallo presentaron mayor porcentaje de inhibición contra los diferentes tipos de Fusarium evaluados por el método de dilución en agar. Los extractos de V. mollis inhibieron a F. verticillioides entre 62 y 91 % con 120 μg mL-1 de extracto. El orden de las especies de hongos inhibidas por los extractos fue: F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. La composición química de las especies se determinó mediante pruebas para fenoles, taninos, flavonoides, triterpenos, alcaloides, cumarinas y saponinas. Ninguno de los extractos presentó alcaloides y saponinas. Los fenoles (37.1 mg EAG/g muestra seca) y flavonoides (26.8 mg EQ/g muestra seca) fueron los compuestos mayoritarios en los extractos metanólicos y acuosos. En conclusión, se requieren cantidades muy pequeñas de extracto para la inhibición de bacterias y de Fusarium; por lo tanto, V. mollis puede ser considerada una fuente de metabolitos para este fin y en la agricultura como control alternativo dentro de un manejo integrado de enfermedades.

Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

2020 ◽  
Vol 24 (19) ◽  
pp. 2272-2282
Author(s):  
Vu Ngoc Toan ◽  
Nguyen Minh Tri ◽  
Nguyen Dinh Thanh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Selenium Dioxide ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal Activities ◽  
Alkyl Ethers ◽  
Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

Comparison of the Effects of Silver in Nanostructured and Ultrahigh Diluted Form on Growth and Volatile Compounds Produced by Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 316-329
Author(s):  
Fateme Mirzajani ◽  
Amin Hamidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Life Cycles ◽  
Gas Chromatography Mass Spectrometry ◽  
Untreated Sample ◽  
E Coli ◽  
Volatile Metabolites ◽  
Octyl Acetate ◽  
Volatile Organic ◽  
And Staphylococcus Aureus

Introduction: In this project, the growth and volatile metabolites profiles of Escherichia coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical, nanoparticle and ultra-highly diluted compounds. Materials & Methods: The treatments were done for 12000 life cycles using silver nanoparticles (AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later. Also, with the continuous usage of these materials, no more bacterial growth was observed. Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate, Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol. The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde, 2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were common and produced by both organisms. Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).

TiO2 antifouling coating based on epoxy-modified tung oil waterborne resin

2021 ◽  
pp. 096739112110111
Author(s):  
Hailiang Hu ◽  
Minmin Chen ◽  
Mengye Cao
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Survival Rate ◽  
Light Irradiation ◽  
Fouling Resistance ◽  
Tung Oil ◽  
E Coli ◽  
Antifouling Coating ◽  
Waterborne Epoxy

The eco-friendly functionalized TiO2/polymer antifouling (AF) coating was successfully synthesized by dispersing TiO2 nanoparticles in waterborne epoxy-modified tung oil resin. The AF effectiveness of coating was evaluated toward Staphylococcus aureus ( S. aureus, ATCC6538), Escherichia coli ( E. coli, ATCC8739) and diatom ( Cyclotella sp., FACHB-1635). The nanoTiO2/polymer AF coating showed good antimicrobial activity both under the light and dark conditions by comparison with the pristine TiO2 nanoparticles and bulk polymer. Under light irradiation for 50 min, the AF coating showed only 8.4% and 8% survival rate for S. aureus and E. coli. In addition, The AF coatings exhibited favorable inhibition efficacy toward the growth and adhesion of Cyclotella sp., and the efficacy was enhanced with the increase of TiO2 content. It can be concluded that TiO2 nanoparticles endow the AF coatings with promoted fouling resistance properties.

scholarly journals Fabrication of a Novel AgBr/Ag2MoO4@InVO4 Composite with Excellent Visible Light Photocatalytic Property for Antibacterial Use

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1541
Author(s):  
Jie Zhang ◽  
Jia Wang ◽  
Qingjun Zhu ◽  
Binbin Zhang ◽  
Huihui Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Degradation Rate ◽  
Photocatalytic Property ◽  
Photocatalytic Reaction ◽  
Composite Photocatalyst ◽  
E Coli ◽  
Radical Trapping ◽  
Visible Light Photocatalytic

A novel AgBr/Ag2MoO4@InVO4 composite photocatalyst with different heterojunction structures was successfully constructed by compounding InVO4 with Ag2MoO4 and AgBr. According to the degradation, antibacterial and free radical trapping data, the photocatalytic antibacterial and antifouling activities of AgBr/Ag2MoO4@InVO4 composite were evaluated, and the corresponding photocatalytic reaction mechanism was proposed. Adding AgBr/Ag2MoO4@InVO4 composite, the degradation rate of ciprofloxacin (CIP) achieved 95.5% within 120 min. At the same time, the antibacterial rates of Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) achieved 99.99%. The AgBr/Ag2MoO4@InVO4 composite photocatalyst showed promising usage in photocatalytic antibacterial and purification areas.

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