scholarly journals ИЗОГЕНЕЗ ЕКСПЛАНТІВ SILENE HYPANICA KLOKOV ТА ЇХ АДАПТАЦІЯ ДО УМОВ EX VITRO

Author(s):  
L. L. Dzhus ◽  
L. A. Koldar

Among the conservation measures related to the preservation of plant diversity, an important place belongs to the creation and preservation of rare and endangered species in the crop conditions. Thus, the species Silene hypanica Klokov is under threat of extinction. This relict and endemic species is listed in the Red book of Ukraine, the European Red list, and 6th Resolution of the Berne Convention. Prospects for the conservation of this species are related to the study of its viability and require the development of effective methods of reproduction, in particular in vitro crop. The article presents the results of studies of one of the breeding stages in vitro – rhizogenesis of S. hypanica esplants and its dependence on the concentrations of β-indolyl butyric acid in nutrient media (0,1; 0,5; 1,0; 1,5; 2,0 mg / l), followed by adaptation of regenerating plants to ex vitro conditions. The study showed that the active process of rhizogenesis occurred due to the addition of β-IMC to the nutrient medium at a concentration of 0.5 mg/l and contributed to the production of 86.4 % of rooted regenerating plants. Transplanting rooted plants into peat disks and then growing them in containers contributed to the successful adaptation of rooted plants.

Author(s):  
V.K. Karimova ◽  
◽  
B.N. Baktybai ◽  
G.K. Magzumova ◽  
ZH. T. Sartbaev ◽  
...  

Today, many living organisms are negatively affected by climate change and anthropogenic activities, which leads to a decrease in their numbers. One of these rare and endangered plant species is the Ili barberry (Berberis iliensis) and the Karkaraly barberry (Berberis karkaralensis). This work is devoted to the optimization study of the cultivation conditions for a rare and endangered species of Ili barberry and Karkaralinsky barberry in vitro. To obtain sterile and viable explants, the sterilizing agent was a solution of 0.5% «Domestos» (7 min). For the regeneration of the main shoot of the barberry, the optimal nutrient medium is Murashige and Skoogwith the addition of 6-benzylaminopurine- 0.5 mg/l, gibberellic acid- 1.0 mg/l, indole-3-butyric acid -0.01 mg/l, where regeneration was 80% for the Ili barberry, barberry karkaralinsky - 70%. For the multiplication of Berberis iliensis microshoots, the Quoirin & Lepoivre culture medium with the addition of 0.75 mg/l - benzylaminopurine is optimal; the number of microshoots formed was 3.6 per explant. Root formation is one of the most difficult stages in micropropagation. For the rooting of microshoots of Karkaralinsky barberry, a nutrient medium of ½ Murashige and Skoog was used with the addition of indolylbutyric acid -1.5 mg/l.


Biologija ◽  
2019 ◽  
Vol 65 (1) ◽  
Author(s):  
Oksana Chornobrov ◽  
Svitlana Bilous ◽  
Oleksandr Chornobrov ◽  
Maria Manko

Conservation and reproduction of rare genotypes of Salix L. species, in particular the blunt-leaved willow (Salix retusa L.) and Jacquin’s Willow (Salix alpina Scop.) that are listed in the Red Data Book of Ukraine in the status of rare and endangered species, is one of the urgent tasks of the present. The aim of research was to develop methods of introduction of S. retusa and S. alpina into in vitro culture for their mass reproduction and conservation. The plant material was cultivated on a culture medium prescribed by MS, WPM, and DKW with the addition of growth regulators according to the conventional method. Effective sterilization (over 80%) of explants of S. retusa and S. alpina was achieved by applying a stepwise method, which consisted of consistently maintaining them in solutions 0.1% HgCl2 and 1.0% AgNO3 for 5–6 min. Significant results in the regeneration of explants by activating the growth of available meristems in vitro were observed on MS with the addition of 0.25–0.5 mg/l 6-(Furfurylaminopurine, kinetin) and 2 g/l activated carbon. Our further research will serve as a base for developing microclonal propagation of S. retusa and S. alpina for their conservation and reproduction in vitro.


2008 ◽  
Vol 60 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Snezana Simic

Data about distribution of Bangia atropurpurea (Roth) C. Agardh indicate that this species was found on relatively small number of localities in freshwaters in Europe and world. In Red List of Algae, in some countries, this species is defined as a extinct (Ex) (Poland) or as endangered (En) (Slovakia). In this study, morphological and ecological characteristics of rare and endangered species B. atropurpurea that was found on three localities in Serbia: in Trgoviski Timok River (East Serbia), Gvozdacka Reka River (West Serbia) and Raska River (South-western Serbia). The Raska River is new find of the species B. atropurpurea.


2015 ◽  
Vol 70 (2) ◽  
pp. 85-96
Author(s):  
MAŁGORZATA REKIEL ◽  
JANINA SKRZYCZYŃSKA ◽  
MARIA ŁUGOWSKA

The aim of the study was to make an inventory and describe the localities of interesting species inhabiting the segetal communities of the Turobin commune, which are regarded as endangered in Poland and Europe. The paper presents results of a field study conducted in 2011-2013 in crop fields, located on brown soils and rendzinas. Phytosociological relevés were made with the Braun-Blanquet method at the sites of phytocoenoses comprising interesting and endangered species. The habitat was assessed with the use of soil-agricultural maps, and soil pH was measured at the site where the phytosociological relevés were taken. In the Turobin commune, 32 interesting species were found; some of them are considered rare and endangered in Poland. In this group, the occurrence of Asperugo procumbens, regarded as an endangered species and included in the red list of vascular plants, is noteworthy.


2014 ◽  
Vol 21 (5) ◽  
pp. 601-609
Author(s):  
Wang Deyun ◽  
Peng Jie ◽  
Chen Yajing ◽  
Lü Guosheng ◽  
Zhang Xiaoping ◽  
...  

2020 ◽  
Vol 957 (3) ◽  
pp. 47-53
Author(s):  
E.A. Kravets

The author offers mapping and geoecological analysis of the Russian Federation regions presence in the state program “Environmental Protection”. The unequal distribution of the program’s targets and activities in different regions is revealed. A considerable number of relevant environmental problems for several mentioned regions have not been reflected in the program. It is important to increase the area of specially protected natural areas for a significant number of subjects of the Russian Federation. The status “part of the territory occupied by specially protected natural territories of Federal value in the total area of the subject of the Russian Federation” is recommended to be assigned all regions of Russia. Identification and elimination of objects of accumulated environmental damage that threat to the Volga river is relevant, at least for all the regions in which the Volga flows. Not all regions with a high level of air pollution and/or large masses of air pollutants have the indicator “reduction of total emissions for the reporting year”. It is necessary to increase the Program of measures for the protection of rare and endangered species of plants and to expand the list of regions in which it is planned to protect rare and endangered species of animals.


2021 ◽  
Vol 13 (6) ◽  
pp. 3007
Author(s):  
Xiaojiong Zhao ◽  
Jian Wang ◽  
Junde Su ◽  
Wei Sun ◽  
Haoxian Meng

Quantitative assessment and evaluation of ecological parameters and biodiversity conservation are prime concerns for long-term conservation of rare and endangered species and their associated habitats in any ecological region. In this study, Gansu Province, a biodiversity hotspot, was chosen as the research area. We predicted the distribution patterns of suitable habitats for rare and endangered species. The replacement cost method was adopted to calculate the conservation value of rare and endangered species. The suitable habitat distribution area of rare and endangered wild animals reached 351,607.76 km2 (without overlapping area), while that of plants reached 72,988.12 km2 (without overlapping area). The conservation value of rare and endangered wildlife is US $1670.00 million. The high-value areas are mostly concentrated in the south and north of Gansu Province. The conservation value of rare and endangered wild plants is US $56,920.00 million. The high-value areas are mostly concentrated south of Gansu Province. The conservation value is US $58,590.00 million a year, and its distribution trend is gradually decreasing from northeast to southwest, with the highest in the forest area south of Gansu Province, followed by the Qilian Mountain area in the north. These results are of great significance for future improvement of the evaluation index system of ecosystem services and the development of ecosystem services and management strategies.


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