scholarly journals Bacterial Community of Neutral Mine Drainage of Elizabeth’s Shaft (Slovinky, Slovakia)

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Jana KISKOVÁ ◽  
Zuzana PERHÁČOVÁ ◽  
Ladislav VLČKO ◽  
Jana SEDLÁKOVÁ-KADUKOVÁ ◽  
Simona KVASNOVÁ ◽  
...  
Keyword(s):  
Bacterial Community ◽  
454 Pyrosequencing ◽  
Bacterial Population ◽  
Mine Drainage ◽  
Ph Value ◽  
Drainage Water ◽  
Rrna Gene ◽  
High Concentration ◽  
Neutral Mine Drainage ◽  
High Level

Neutral mine drainage is the less frequent subject of the interest than acid mine drainage but it can have adverse environmental eff ects caused mainly by precipitation of dissolved Fe. Th e aim of the study was to characterize the composition of bacterial population in environment with high concentration of iron and sulfur compounds represented by neutral mine drainage water of Elizabeth’s shaft , Slovinky (Slovakia). Th e pH value of drainage water decreased from 7.1 to 6.5 during the years 2008–2014. Direct microscopic observations, cultivation methods, and 454 pyrosequencing of the 16S rRNA gene amplicons were used to examine the bacterial population. Microscopic observations identifi ed iron–oxidizing Proteobacteria of the genera Gallionella and Leptothrix which occurrence was not changed during the years 2008–2014. Using 454 pyrosequencing, there were identifi ed members of 204 bacterial genera that belonged to 25 phyla. Proteobacteria (69.55%), followed by Chlorofl exi (10.31%) and Actinobacteria (4.24%) dominated the bacterial community. Genera Azotobacter (24.52%) and Pseudomonas (14.15%), followed by iron–oxidizing Proteobacteria Dechloromonas (11%) and Methyloversatilis (8.53%) were most abundant within bacterial community. Typical sulfur bacteria were detected with lower frequency, e.g., Desulfobacteraceae (0.25%), Desulfovibrionaceae (0.16%), or Desulfobulbaceae (0.11%). Our data indicate that the composition of bacterial community of the Elizabeth’s shaft drainage water refl ects observed neutral pH, high level of iron and sulfur ions in this aquatic habitat.

scholarly journals Correction to: The Bacterial Population of Neutral Mine Drainage Water of Elizabeth’s Shaft (Slovinky, Slovakia)

2020 ◽  
Vol 77 (8) ◽  
pp. 1957-1957
Author(s):  
Jana Kisková ◽  
Zuzana Perháčová ◽  
Ladislav Vlčko ◽  
Jana Sedláková ◽  
Simona Kvasnová ◽  
...  
Keyword(s):  
Open Access ◽  
Bacterial Population ◽  
Mine Drainage ◽  
Drainage Water ◽  
Internet Portal ◽  
Creative Commons ◽  
Link Type ◽  
Neutral Mine Drainage

The article “The Bacterial Population of Neutral Mine Drainage Water of Elizabeth's Shaft (Slovinky, Slovakia)”, written by Jana Kisková, Zuzana Perháèová, Ladislav Vlèko, Jana Sedláková, Simona Kvasnová and Peter Pristaš, was originally published electronically on the publisher’s internet portal on 12 March 2018 without open access. With the author(s)’ decision to opt for Open Choice the copyright of the article changed on 9 April 2020 to © The Author(s) 2018 and the article is forthwith distributed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The original article has been corrected.

scholarly journals The Bacterial Population of Neutral Mine Drainage Water of Elizabeth’s Shaft (Slovinky, Slovakia)

2018 ◽  
Vol 75 (8) ◽  
pp. 988-996 ◽  
Author(s):  
Jana Kisková ◽  
Zuzana Perháčová ◽  
Ladislav Vlčko ◽  
Jana Sedláková ◽  
Simona Kvasnová ◽  
...  
Keyword(s):  
Bacterial Population ◽  
Mine Drainage ◽  
Drainage Water ◽  
Neutral Mine Drainage

scholarly journals Dynamics of Fermentation Parameters and Bacterial Community in High-Moisture Alfalfa Silage with or without Lactic Acid Bacteria

2021 ◽  
Vol 9 (6) ◽  
pp. 1225
Author(s):  
Shanshan Zhao ◽  
Fengyuan Yang ◽  
Yuan Wang ◽  
Xiaomiao Fan ◽  
Changsong Feng ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacterial Community ◽  
Ph Value ◽  
Ammoniacal Nitrogen ◽  
High Concentration ◽  
High Moisture ◽  
Acid Accumulation ◽  
Alfalfa Silage ◽  
Fermentation Parameters

The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH3-N); Enterobacter and Hafnia-Obesumbacterium were the dominant genera. At 60 d, the pH value and the concentration of NH3-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and Garciella had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH3-N except at 60 d, and significantly promoted the abundance of Lactobacillus and decreased the abundance of Garciella and Anaerosporobacter, but did not significantly inhibit the growth of Enterobacter and Hafnia-Obesumbacterium. In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.

scholarly journals Changes in the Composition of the Soil Bacterial Community in Heavy Metal-Contaminated Farmland

2021 ◽  
Vol 18 (16) ◽  
pp. 8661
Author(s):  
Shu-chun Tseng ◽  
Chih-ming Liang ◽  
Taipau Chia ◽  
Shan-shin Ton
Keyword(s):  
Heavy Metal ◽  
Bacterial Community ◽  
Structural Changes ◽  
Ph Value ◽  
Deep Layer ◽  
Soil Bacterial Community ◽  
Rrna Gene ◽  
Zinc Tolerance ◽  
Copper And Zinc ◽  
Soil Bacterial

The structural changes of microorganisms in soil are the focus of soil indicators research. The purpose of this study was to investigate the changes in the composition of the soil bacterial community in heavy metal-contaminated soil. A total of six soil samples (two sampling times) were collected from contaminated farmland at three different depths (surface, middle, and deep layer). The pH value was measured. The concentrations of heavy metals (Cr, Ni, Cu, Zn, Cd, and Pb) and the soil bacterial community were analyzed using ICP-OES and 16S rRNA gene sequencing. The results of the two samplings showed that the pH value in the deep layer decreased from 6.88 to 6.23, and the concentrations of Cu, Zn, Cd, and Pb, with a smaller ion radius, increased by 16–28%, and Shannon, Chao1 increased by ~13%. The bacteria community composition at the three depths changed, but Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla. In the copper and zinc tolerance test, the isolated bacterium that was able to tolerate copper and zinc was Bacillus sp. We found that, the longer the heavy metal pollution was of concern, the higher the tolerance. These results can be used as references for the microbial remediation of heavy metal-contaminated farmland.

Bacterial community composition of anthropogenic biochar and Amazonian anthrosols assessed by 16S rRNA gene 454 pyrosequencing

2013 ◽  
Vol 104 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Rodrigo Gouvêa Taketani ◽  
Amanda Barbosa Lima ◽  
Ederson da Conceição Jesus ◽  
Wenceslau Geraldes Teixeira ◽  
James M. Tiedje ◽  
...  
Keyword(s):  
Bacterial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Community Composition ◽  
454 Pyrosequencing ◽  
Bacterial Community Composition ◽  
Rrna Gene

Roles of Oligotrophic Acidophiles (Alicyclobacillus) in Chalcopyrite Bioleaching System: Shake Flask Evaluation

2015 ◽  
Vol 1130 ◽  
pp. 410-413
Author(s):  
Xing Yu Liu ◽  
Ming Jiang Zhang ◽  
Wen Yan Liu ◽  
Bo Wei Chen ◽  
Chun Yu Meng ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Bacterial Community ◽  
Mine Drainage ◽  
Community Change ◽  
Rrna Gene ◽  
Acid Mine ◽  
Copper Leaching ◽  
Culture Independent ◽  
Different Temperatures ◽  
Two Temperatures

An oligotrophic culture of acidophiles (Alicyclobacillus) isolated from Dexin acid mine drainage was evaluated for its synergistic effect in chalcopyrite bioleaching. Bioleaching of chalcopyrite with and without theAlicyclobacillusculture was investigated at different temperatures (33°C, 45 °C and 65 °C) and a culture-independent approach based on 16S rRNA gene clone library was used to analyze changes in the microbial community change during the bioleaching process. For 33oC leaching tests, only the bacterial community was analyzed, but for the other two temperatures, both the bacterial community and archaea communities were analyzed. Results showed that at high leaching temperature (65°C),Alicyclobacillusculture could increase copper leaching recovery from 57.83% to 60.7%. While at relative low temperature (45°C and 33°C), addingAlicyclobacillusculture inhibited copper bioleaching, copper leaching recovery decreased from 36.10% to 31.52% and from 34.02% to 21.97% respectively at 45°C and 33°C. Clone libraries analysis showed thatAlicyclobacillushelps the growth of genusSulfobacillusat 45 °C while inhibiting the growth of genusLeptospillumat both 33°C and 45 °C. Furthermore, when addingAlicyclobacillusgrowth ofFerroplasmawas limited andAcidoplasmawas facilitated at 45°C. At 60°C, addingAlicyclobacillusculture facilitated the growth of genusMetallosphaerawhile limiting the growth ofLeptospillumandFerroplasma. The results showed potential application ofAlicyclobacillusin high temperature chalcopyrite bioleaching and bioremediation of acid mine drainage.

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