scholarly journals Length polymorphisms of simple sequence repeat (SSR) markers in assisted selection for lipoxygenase-free in soybean

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
VALERIA CARPENTIERI-PIPOLO ◽  
Thales Pereira Barreto ◽  
Daiana Alves Silva ◽  
Ricardo Vilela Abdelnoor ◽  
Silvana Rockenbach Marin ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Null Allele ◽  
Seed Quality ◽  
Soybean Seed ◽  
Hybrid Population ◽  
Human Consumption ◽  
F1 Hybrid ◽  
Hybrid Plants ◽  
Length Polymorphisms ◽  
Glycine Max L

Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified.  The SSR markers Sat090 and Sat417 was the most effective diagnostic marker among the all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in The F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.

scholarly journals Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Kulpash Bulatova ◽  
Shynar Mazkirat ◽  
Svetlana Didorenko ◽  
Dilyara Babissekova ◽  
Mukhtar Kudaibergenov ◽  
...  
Keyword(s):  
Trypsin Inhibitor ◽  
Seed Quality ◽  
Soybean Seed ◽  
Seed Proteins ◽  
Germplasm Collection ◽  
F1 Hybrid ◽  
Soybean Germplasm ◽  
Hybrid Plants ◽  
Very High ◽  
Two Hybrid

A soybean germplasm collection was studied for the identification of accessions with low trypsin inhibitor content in seeds. Twenty-nine accessions, parental plants, and two hybrid populations were selected and analyzed using genetic markers for alleles of the Ti3 locus, encoding Kunitz trypsin inhibitor (KTI). Most of the accessions had high or very high KTI (49.22–73.53 Trypsin units inhibited (TUI/mg seeds), while the two local Kazakh cultivars, Lastochka and Ivushka, were found to have a moderately high content of KTI, at 54.16–54.87 TUI/mg. In contrast, two soybean cultivars from Italy, Hilario and Ascasubi, showed the lowest levels of trypsin units inhibited, at 25.47–27.87 TUI/mg. Electrophoresis of seed proteins in a non-denaturing system showed a simple discrimination pattern and very clear presence/absence of bands corresponding to KTI. The SSR marker Satt228 was the most effective diagnostic marker among the three examined, and it confirmed the presence of the homozygous null-allele ti3/ti3 in cultivars Ascasubi and Hilario, which were used for hybridization with the local cv. Lastochka. Heterozygote F1 hybrid plants and homozygous ti3/ti3 lines in F2 segregating populations were successfully identified using Satt228. Finally, through marker-assisted selection with Satt228, prospective homozygous ti3/ti3 lines were produced for further application in the breeding program aimed at improving soybean seed quality in Kazakhstan.

scholarly journals Development of highly polymorphic EST-SSR markers and segregation in F1 hybrid population of Vitis vinifera L.

2013 ◽  
Vol 12 (3) ◽  
pp. 3871-3878 ◽  
Author(s):  
E. Kayesh ◽  
Y.Y. Zhang ◽  
G.S. Liu ◽  
N. Bilkish ◽  
X. Sun ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Hybrid Population ◽  
Vitis Vinifera L ◽  
F1 Hybrid

scholarly journals Yield and chemical composition of soybean seed under different irrigation regimes in the Vojvodina region  

2017 ◽  
Vol 63 (No. 1) ◽  
pp. 34-39 ◽  
Author(s):  
Kresović Branka ◽  
Gajic Bosko Andrija ◽  
Tapanarova Angelina ◽  
Dugalić Goran
Keyword(s):  
Chemical Composition ◽  
Seed Quality ◽  
Soybean Seed ◽  
Sprinkler Irrigation ◽  
Temperate Climate ◽  
Soybean Yield ◽  
Climate Conditions ◽  
Irrigation Regimes ◽  
Glycine Max L ◽  
Irrigation Treatments

The goal of the present research is to determine an effective sprinkler irrigation strategy for soybean [Glycine max (L.) Merr.] in temperate climate conditions, in order to maximize yields and seed quality. A three-year field experiment with four different irrigation treatments was conducted on Calcic Chernozem in the Vojvodina region of Serbia. The irrigation regimes included: no irrigation; full irrigation (I<sub>100</sub>); and two deficit irrigation treatments – 65% of I<sub>100</sub> (I<sub>65</sub>) and 40% of I<sub>100</sub>. The irrigation treatments generally had a statistically significant effect on the increase of soybean yield and protein content. Irrigation did not have a significant effect on the oil content. In general, irrigation increased K, P, Mg, Mn, Cu, Zn and B concentrations and decreased Ca and Fe concentrations in soybean seed. The results show that irrigation with the largest amount of water (treatment I<sub>100</sub>) provided no potential benefit in terms of soybean yield and chemical composition. Treatment I<sub>65</sub>, which exhibited the most favourable watering conditions, is the best choice to maximize yield and ensure a good chemical composition of soybean under these agroecological conditions.  

Effects of Ca2+- and Mg2+-enriched germination media on germination and sugar leakage of Ca-deficient soybean seed

1995 ◽  
Vol 75 (2) ◽  
pp. 343-346 ◽  
Author(s):  
J. R. Keiser ◽  
R. E. Mullen ◽  
P. N. Hinz
Keyword(s):  
High Performance ◽  
Seed Quality ◽  
Sugar Content ◽  
Membrane Integrity ◽  
Soybean Seed ◽  
Bathing Solution ◽  
Distilled Water ◽  
Glycine Max L ◽  
Calcium Magnesium

Inadequate seed Ca concentrations are associated with reduced seed quality of soybean [Glycine max (L.) Merr.]. Standard germination and sugar leakage tests were conducted to determine the effect of seed Ca content and exogenous Ca2+ or Mg2+ on seed quality and seed leakage. Soybean seeds containing different Ca levels were germinated in rolled towels that had been soaked in distilled water, 0.01 M Ca(NO3)2, or 0.01 M Mg(NO3)2. Sugar leakage was measured by soaking seed containing 1.10, 1.72 or 2.36 mg g−1 Ca in distilled water, 0.01 M Ca (NO3)2, 0.01 M Mg(NO3)2 or polyethylene glycol (PEG). An aliquot of the bathing solution was analyzed for sugar content after 6, 12 and 24 h of soaking, by using high-performance liquid chromatography. As seed Ca content decreased from 2.36 to 1.10 mg g−1, germination in distilled water decreased from 87 to 56%. Addition of either Ca(NO3)2 or Mg(NO3)2 to the medium resulted in increased germination of low-Ca seed, but Ca(NO3)2 was more effective in improving germination of low-Ca seed than Mg(NO3)2. Total sugar leakage was significantly greater in low-Ca seed than in high-Ca seed. Bathing solutions of Ca(NO3)2 or Mg(NO3)2 significantly decreased sugar leakage, compared with distilled water or PEG. These results suggest that the role of Ca in seed germination may involve membrane stabilization. They also indicate that the addition of Ca(NO3)2 or Mg(NO3)2 to the germination medium may improve germination of Ca-deficient seed. Key words: Soybean, germination, calcium, magnesium, sugar leakage, membrane integrity

scholarly journals Characterization of F1 interspecific hybrids between wild Helianthus annuus L. populations and cultivated sunflower

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Sreten Terzic ◽  
Jovanka Atlagic ◽  
Dejana Pankovic
Keyword(s):  
Helianthus Annuus ◽  
Ssr Markers ◽  
Interspecific Cross ◽  
F1 Hybrids ◽  
Interspecific Crosses ◽  
Phenotypic Traits ◽  
F1 Hybrid ◽  
Helianthus Annuus L ◽  
Hybrid Plants ◽  
Pollen Vitality

Phenotype, chromosomes pairing and pollen vitality were compared between parental populations and F1 hybrids of interspecific cross between Helianthus annuus L. and cultivated sunflower. The investigation of the simple sequence repeats (SSR) polymorphism was also used to test the hybrid nature of F1 populations. The phenotypic traits of F1 hybrid plants were either closer to the wild species or intermediate. Irregular chromosome pairing was found in only 0 to 10% of meiocytes in the meiosis of F1 hybrid plants. Interspecific crosses were confirmed with SSR markers in all hybrid combinations. Alleles that were not present in parental DNA were frequently observed in F1 hybrids. That is additional evidence that those hybrid combinations were not produced by self-fertilization. The results suggest that SSR markers can be efficiently used for the F1 hybrid characterization in crosses between closely related species, in which, the changes of phenotype, meiosis and pollen vitality are not always significant.

scholarly journals Use of chlrophyll fluorescence sorting to improve soybean seed quality

2009 ◽  
Vol 31 (4) ◽  
pp. 145-151 ◽  
Author(s):  
Silvio Moure Cicero ◽  
Rob Van Der Schoor ◽  
Henk Jalink
Keyword(s):  
Chlorophyll Fluorescence ◽  
Glycine Max ◽  
Seed Quality ◽  
Soybean Seed ◽  
Climatic Conditions ◽  
Fluorescence Technique ◽  
Sorting Technique ◽  
Glycine Max L ◽  
Sorting Process

The occurrence of green seeded soybeans [Glycine max (L.) Merrill] is a problem closely related to unfavorable climatic conditions, mainly drought, that occurs during the final stages of seed maturation. This problem causes serious losses to soybean seed quality in Brazil. In these seeds, chlorophyll is not properly degraded during maturation, drastically reducing seed quality. Using the chlorophyll fluorescence technique, it is possible to remove green seeds from the seed lot, improving seed quality in several species in which the occurrence of green seeds is also a problem. The objective of this research was to study the use of the chlorophyll fluorescence technique in sorting green seeds from soybean seed samples and its effects on quality. Five seed samples of soybean, cultivar TMG 113 RR, with 0%, 5%, 10%, 15%, and 20% of green seeds were used in this study. Seeds from each sample were sorted into two fractions based on the chlorophyll fluorescence signals and then compared to the control (non-sorted seeds). The sorting process showed great differences between the low and high chlorophyll fluorescence fractions. It was concluded that: green seeds of soybeans present high chlorophyll fluorescence and that this characteristic affects the quality of the seeds; it is possible to improve the quality of soybean seed by removing green seeds using the chlorophyll fluorescence sorting technique.

Marker-assisted stacking of null Kunitz trypsin inhibitor and off-flavour generating lipoxygenase-2 in soybean

2021 ◽  
pp. 1-9
Author(s):  
V. Kumar ◽  
A. Rani ◽  
A. K. Anshu ◽  
T. Tayalkar
Keyword(s):  
Ssr Markers ◽  
Trypsin Inhibitor ◽  
Null Allele ◽  
Protein Digestibility ◽  
Null Alleles ◽  
Human Consumption ◽  
Recurrent Parent ◽  
Kunitz Trypsin Inhibitor ◽  
Soybean Genotypes ◽  
Parental Combination

Abstract Soybean varieties genetically free from Kunitz trypsin inhibitor (KTI) and lipoxygenase-2 (Lox2) are desirable to increase human consumption, as the former is an antinutritional factor that affects protein digestibility while the latter is a principal contributor to off-flavour. In the present investigation, soybean genotypes free from both these undesirable components were developed by introgression of null allele of Lox2 from NRC109 (lox2lox2) into two KTI-free soybean genotypes derived from genotypes JS97-52 and NRC7. Foreground selection of plants in F1, F2, BC1F1, BC1F2, BC2F1 and BC2F2 generations developed from two cross combinations i.e. NRC7-derived KTI-free genotype (N7KTIF)×NRC109 (parental combination 1) and JS97-52 derived KTI free genotype (JKTIF)×NRC109 (parental combination 2) was performed using null allele specific markers and tightly linked simple sequence repeat (SSR) markers for both KTI and Lox2 genes for the identification of homozygous recessive (titilox2lox2) plants. Background selection was performed using 239 and 241 polymorphic SSR markers across the genome. This resulted in the development of 9 and 13 soybean lines stacked for null alleles of both KTI and Lox2 (titilox2lox2) exhibiting recurrent parent genome content more than 97 and 96%, respectively. Days-to-flowering, days-to-maturity, 100-seed weight and yield per plant of the stacked lines developed from both the parental combinations were at par with the respective recurrent parents.

Seed quality response of soybean to weed control timing using glyphosate

2004 ◽  
Vol 84 (4) ◽  
pp. 1213-1219
Author(s):  
S. Y. C. Essah ◽  
U. R. Bishnoi
Keyword(s):  
Seed Germination ◽  
Weed Control ◽  
Seed Quality ◽  
Soybean Seed ◽  
Dry Weight ◽  
Cold Test ◽  
Southeast United States ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Quality Response

Field and laboratory studies were conducted during 1997 and 1998 in the southeast United States to determine the influence of timing of glyphosate application on seed germination (standard germination and germination index) and vigor (cold test emergence, accelerated aged emergence, and seed conductivity) of three glyphosate-resistant soybean [Glycine max (L.) Merr.] cultivars that belong to different maturity groups (MG). The soybean cultivars used were H4994 (MG IV — early maturing), H5164 (MG V — medium maturing) and H6686 (MG VI — late maturing). Weeds were controlled by glyphosate applied at 1.1 kg a.i. ha-1 at 2, 3, 4, 5, 6, or 7 wk after soybean emergence (WAE). Using glyphosate to control weeds at 5 WAE or later in the early- and medium-maturing cultivars (H4994 and H5164) results in soybean plants able to produce seeds with more vigor and germinability than when weeds are removed earlier. For late-maturing cultivar H6686, weed control at 4 WAE produces seeds with higher germination and vigor than when weeds are removed later. Key words: Glyphosate, weed control timing, soybean, seed germination and vigor, weed dry weight

scholarly journals The Use of Electrostatic Field to Improve Soybean Seed Germination in Organic Production

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1473
Author(s):  
Zlatica Mamlic ◽  
Ivana Maksimovic ◽  
Petar Canak ◽  
Goran Mamlic ◽  
Vojin Djukic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Electrostatic Field ◽  
Seed Quality ◽  
18Th Century ◽  
Soybean Seed ◽  
Organic Production ◽  
Initial Growth ◽  
Physical Treatment ◽  
Soybean Seedlings ◽  
Dark Green

Soybean production in the system of organic agriculture is not very demanding, and this has been well documented both through experimental results and commercial production. However, one of the biggest problems in organic production is the lack of adequate pre-sowing treatments. Therefore, the aim of this study was to examine the effect of the electrostatic field. This is a physical treatment that was first used for seed treatment in the 18th century but has mostly been neglected since then. Seeds of five soybean genotypes with differently colored seed coats (yellow, green, dark green, brown, and black) were included in this study. The seeds were exposed to different values of direct current (DC) with the following voltages: 0 V (control), 3 V, 6 V, and 9 V, to which the seeds were exposed for 0 min (control), 1 min, and 3 min. After exposing the seeds to the electric field, the physiological properties of seeds and seedlings at the first stage of growth were evaluated. The results show that the effect of the electrostatic field on seed quality depends on the genotype, voltage, and exposure time. The application of DC can be a suitable method for improving seed germination and the initial growth of soybean seedlings. In addition, the results indicate that it is necessary to adjust the DC treatment (voltage and duration of exposure of seeds) to particular genotypes since inadequate treatments may reduce the quality of seeds.

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