scholarly journals Development of highly polymorphic EST-SSR markers and segregation in F1 hybrid population of Vitis vinifera L.

2013 ◽  
Vol 12 (3) ◽  
pp. 3871-3878 ◽  
Author(s):  
E. Kayesh ◽  
Y.Y. Zhang ◽  
G.S. Liu ◽  
N. Bilkish ◽  
X. Sun ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Hybrid Population ◽  
Vitis Vinifera L ◽  
F1 Hybrid

scholarly journals Characterization of EST-derived and non-EST simple sequence repeats in an F1 hybrid population of Vitis vinifera L.

2014 ◽  
Vol 13 (1) ◽  
pp. 2220-2230 ◽  
Author(s):  
E. Kayesh ◽  
N. Bilkish ◽  
G.S. Liu ◽  
W. Chen ◽  
X.P. Leng ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Simple Sequence Repeats ◽  
Hybrid Population ◽  
Vitis Vinifera L ◽  
F1 Hybrid ◽  
Simple Sequence

scholarly journals Length polymorphisms of simple sequence repeat (SSR) markers in assisted selection for lipoxygenase-free in soybean

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
VALERIA CARPENTIERI-PIPOLO ◽  
Thales Pereira Barreto ◽  
Daiana Alves Silva ◽  
Ricardo Vilela Abdelnoor ◽  
Silvana Rockenbach Marin ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Null Allele ◽  
Seed Quality ◽  
Soybean Seed ◽  
Hybrid Population ◽  
Human Consumption ◽  
F1 Hybrid ◽  
Hybrid Plants ◽  
Length Polymorphisms ◽  
Glycine Max L

Beany flavor of soybean (Glycine max (L.) Merr.) is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases (LOX1, LOX2 and LOX3) present in mature seeds. The unpleasant flavor restricts human consumption of soybean products. This problem could be solved through genetic elimination of alleles that code these enzymes. Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus, encoding Lox1, Lox2 and Lox3 free. The SSR marker Satt212 confirmed the presence of the homozygous null-allele Lx3 in the cultivar BRS 213, which were used for hybridization with BR 36. Heterozygote F1 hybrid plants and homozygous Lx3 lines in F2 segregating populations were successfully identified.  The SSR markers Sat090 and Sat417 was the most effective diagnostic marker among the all SSR markers tested. Satt090 and Satt417 confirmed the presence of the homozygous Lx2 null-allele in the parental cultivar BRS 213 by flanking Lx2 loci at 3,00 and 2,77 cM, respectively. The presence of Lx2 null allele in The F2 segregating populations between BRS 213 and BRS 155 were successfully identified with a selection efficiency of 98% and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.

scholarly journals First characterisation of minor and neglected Vitis vinifera L. cultivars from Mount Etna

2018 ◽  
Vol 45 (No. 1) ◽  
pp. 37-46
Author(s):  
Filippo Ferlito ◽  
Elisabetta Nicolosi ◽  
Stefano La Malfa ◽  
Antonio Cicala ◽  
Alessandra Gentile
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Agronomic Traits ◽  
Germplasm Conservation ◽  
Morphological Diversity ◽  
Molecular Data ◽  
Mount Etna ◽  
Conservation Strategy ◽  
Vitis Vinifera L ◽  
Morphological And Molecular Data

Eight minor and neglected cultivars of Vitis vinifera L. were characterised according to their ampelographic and agronomic traits and discriminated by molecular analysis with SSR markers. These results are the first complete ampelographic description of these minor and neglected cultivars from the Mount Etna region. The results of this study reveal high morphological diversity of ancient grapevines growing in this region. SSR markers enabled us to discriminate the cultivars and revealed the genetic divergence between them and several autochthonous cultivars. Our efforts could contribute to a better knowledge of grape biodiversity based on morphological and molecular data and could be useful for the development of a reliable germplasm conservation strategy. 

Somatic chimerism, genetic inheritance, and mapping of the fleshless berry (flb) mutation in grapevine (Vitis vinifera L.)

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 721-728 ◽  
Author(s):  
L Fernandez ◽  
A Doligez ◽  
G Lopez ◽  
M R Thomas ◽  
A Bouquet ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Bulk Segregant Analysis ◽  
Cell Layer ◽  
Vitis Vinifera L ◽  
Sequence Repeat ◽  
Wild Type ◽  
Target Region ◽  
Simple Sequence

The fleshless berry (flb) mutation of grapevine (Vitis vinifera L. 'Ugni Blanc') impairs the differentiation and division of inner mesocarp cells responsible for flesh in grapevine berries. In order to study the inheritance of the mutation and to map the flb locus, 5 segregating populations were created. Progeny plants were classified as mutant or wild type by scoring for the presence of an ovary phenotype associated with the Flb– phenotype at anthesis. Phenotypic segregation revealed the involvement of a single dominant allele that was heterozygous in the original mutant. Through bulk segregant analysis, microsatellite (simple sequence repeat (SSR)) markers linked to the mutation were identified, and the flb locus was assigned to linkage group 18. The locus position was then refined by analyzing individual progeny and the segregation of SSR markers in the target region with the closest marker 5.6 cM distant from the flb locus. All progeny with the Flb– ovary phenotype differed from the original fleshless berry mutant in that no berries formed after anthesis. Our data suggest that the original mutant plant was a chimera with the mutated allele present in only 1 cell layer (L2 layer) of the ovary and berry.Key words: Vitis vinifera, mutation, genetic mapping, chimerism.

scholarly journals Urgent need for preservation of grapevine (Vitis vinifera L. subsp. vinifera) germplasm from small circum-Sicilian islands as revealed by SSR markers and traditional use investigations

2016 ◽  
Vol 64 (6) ◽  
pp. 1395-1415 ◽  
Author(s):  
Alessandro Silvestre Gristina ◽  
Roberto De Michele ◽  
Giuseppe Garfì ◽  
Tommaso La Mantia ◽  
Ignazio Fontana ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Ssr Markers ◽  
Vitis Vinifera L ◽  
Traditional Use

Resveratrol oligomers from Vitis Vinifera L. stems

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
H Amira-Guebailia ◽  
T Richard ◽  
S Rouaiguia ◽  
P Waffo Tueguo ◽  
JC Delaunay ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L

Biotransformation of 4-hydroxybenzoic acid in the rhizosphere of grapevine (Vitis vinifera L.)

2017 ◽  
Vol 40 (1) ◽  
pp. 95-102 ◽  
Author(s):  
B. Wang ◽  
T. Zhou1 ◽  
K. Li ◽  
X.W. Guo ◽  
Y.S. Guo ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Hydroxybenzoic Acid

Isolation and characterization of cell walls from the mesocarp of mature grape berries (Vitis vinifera)

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher
Keyword(s):  
Vitis Vinifera ◽  
Cell Walls ◽  
Vascular Tissue ◽  
Compositional Analysis ◽  
Water Soluble ◽  
Vitis Vinifera L ◽  
Nylon Mesh ◽  
Cytoplasmic Material ◽  
Isolation And Characterization ◽  
Tissue Homogenates

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.

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