scholarly journals Oral exfoliative cytology – A technical appraisal in oral diseases

2021 ◽  
Vol 4 (1) ◽  
pp. 115-118
Author(s):  
Sreeshyla HS ◽  
Hegde Usha ◽  
Srinivas J ◽  
Priyanka Nitin ◽  
Premalatha BR
Keyword(s):  
Disease Diagnosis ◽  
Exfoliative Cytology ◽  
Diagnostic Efficiency ◽  
Oral Diseases ◽  
Buccal Cells ◽  
Resource Setting ◽  
Oral Exfoliative Cytology ◽  
Technical Appraisal ◽  
Nuclear Features ◽  
Exfoliated Buccal Cells

Oral exfoliative cytology is one of the easy, simple, economical and non-cumbersome diagnostic adjunct. It involves examining the exfoliated buccal cells for alterations in cellular and nuclear features. It offers the advantage of being useful in less resource setting areas. Though associated with many false results, the newer technological advancements have proven its diagnostic efficiency. Oral exfoliative cytology can readily be used as adjunct to disease diagnosis, however, it cannot replace biopsy.

scholarly journals Rapid detection of Candida albicans in oral exfoliative cytology samples by loop-mediated isothermal amplification

2017 ◽  
Vol 59 (4) ◽  
pp. 541-547 ◽  
Author(s):  
Hiroyasu Noguchi ◽  
Takashi Iwase ◽  
Daisuke Omagari ◽  
Masatake Asano ◽  
Ryota Nakamura ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Exfoliative Cytology ◽  
Loop Mediated Isothermal Amplification ◽  
Oral Exfoliative Cytology

Optical Sectioning of Oral Exfoliated Cells

1972 ◽  
Vol 51 (2) ◽  
pp. 560-568 ◽  
Author(s):  
Robert Hoffman ◽  
Leo Gross
Keyword(s):  
Cell Membrane ◽  
Interference Microscopy ◽  
Exfoliative Cytology ◽  
Optical Sectioning ◽  
Exfoliated Cells ◽  
Oral Exfoliative Cytology

Differential interference microscopy is suggested as an additional technique for oral exfoliative cytology. Optical sectioning provides clear views of the nucleus and other organelles that are not obstructed by underlying or overlying layers. With this technique, interlocking folds in the cell membrane can be observed clearly.

scholarly journals Evaluation of dentists’ knowledge of the use of oral exfoliative cytology in clinical practice

2014 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Weber Adorno Silva ◽  
Ana Paula de Lima ◽  
Luana Marotta Reis De Vasconcellos ◽  
Ana Lia Anbinder
Keyword(s):  
Clinical Practice ◽  
Exfoliative Cytology ◽  
Oral Exfoliative Cytology

scholarly journals Role of micronucleus in oral exfoliative cytology

2015 ◽  
Vol 7 (6) ◽  
pp. 409 ◽  
Author(s):  
BK Akshatha ◽  
R Shashikala ◽  
AP Indira ◽  
GS Manjunath ◽  
KArathi rao
Keyword(s):  
Exfoliative Cytology ◽  
Oral Exfoliative Cytology

scholarly journals Efficacy of Oral Exfoliative Cytology in Diabetes Mellitus Patients: A Light Microscopic and Confocal Microscopic Study

2015 ◽  
Vol 16 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Deepika Gopal ◽  
N Malathi ◽  
B Thirupathi Reddy
Keyword(s):  
Diabetes Mellitus ◽  
Epithelial Cells ◽  
Microscopic Study ◽  
Exfoliative Cytology ◽  
Type 2 Dm ◽  
Buccal Epithelial Cells ◽  
Oral Exfoliative Cytology ◽  
Confocal Microscopic Study ◽  
Qualitative Changes

ABSTRACT Aim Diabetes mellitus (DM) has become a global problem. By monitoring the health status of these individuals, diabetic complications can be prevented. We aimed to analyze alterations in the morphology and cytomorphometry of buccal epithelial cells of type 2 DM patients using oral exfoliative cytology technique and determine its importance in public health screening, diagnosis and monitoring of diabetes mellitus. Materials and methods The study was carried out in 100 type 2 DM patients and 30 healthy individuals. Smears were taken from the right buccal mucosa and stained by the Papanicolaou technique. Staining with Acridine orange was carried out to view qualitative changes with confocal laser scanning microscope (LSM-510 Meta). The cytomorphometry was evaluated using IMAGE PRO PLUS 5.5 software with Evolution LC camera. All findings were statistically analyzed. Results The results showed that with increase in fasting plasma glucose levels, there is significant increase in nuclear area, decrease in cytoplasmic area, and increase in nuclear cytoplasmic ratio (p < 0.05) when compared to the control group. Various qualitative changes were noted, such as cell degeneration, micronuclei, binucleation, intracytoplasmic inclusion, candida and keratinization. Conclusion In the present study, we found significant alterations in the cytomorphometry and cytomorphology of buccal epithelial cells of type 2 DM patients. This study supports and extends the view that these cellular changes can alert the clinician to the possibility of diabetes and aid in monitoring of diabetes throughout the lifetime of the patient. How to cite this article Gopal D, Malathi N, Reddy BT. Efficacy of Oral Exfoliative Cytology in Diabetes Mellitus Patients: A Light Microscopic and Confocal Microscopic Study. J Contemp Dent Pract 2015;16(3):215-221.

scholarly journals Micronucleus Frequency in Exfoliated Buccal Cells of Children Living in an Industrialized Area of Apulia (Italy)

2020 ◽  
Vol 17 (4) ◽  
pp. 1208 ◽  
Author(s):  
Alessandra Panico ◽  
Tiziana Grassi ◽  
Francesco Bagordo ◽  
Adele Idolo ◽  
Francesca Serio ◽  
...  
Keyword(s):  
Biological Effect ◽  
Food Habits ◽  
Human Population ◽  
Anthropogenic Impacts ◽  
Personal Data ◽  
Control Area ◽  
Buccal Cells ◽  
Micronucleus Frequency ◽  
Ultrafine Particulate Matter ◽  
Exfoliated Buccal Cells

Micronuclei (MN) are biomarkers of early biological effect often used for detecting DNA damage in human population exposed to genotoxic agents. The aim of this study was to evaluate the frequency of MN in exfoliated buccal cells of children living in an industrialized (impacted) area compared with that found in children living in a control area without significant anthropogenic impacts. A total of 462 6–8-year-old children (206 in the impacted area, 256 in the control area) attending primary school were enrolled. A questionnaire was administered to the parents of the recruited children to obtain information about personal data, lifestyles, and food habits of their children. Atmospheric particulate fractions were collected near the involved schools to assess the level of environmental exposure of the children. The presence of MN was highlighted in 68.4% of children living in the impacted area with a mean MN frequency of 0.66‰ ± 0.61‰. MN positivity and frequency were significantly lower in the control area (37.1% and 0.27‰ ± 0.43‰, respectively). The frequency of MN was positively associated with quasi-ultrafine particulate matter (PM0.5), traffic near the home, and consuming barbecued food; while adherence to the Mediterranean diet and practicing sport were negatively associated.

Oral exfoliative cytology for the non-invasive diagnosis in X-linked Emery–Dreifuss muscular dystrophy patients and carriers

1998 ◽  
Vol 8 (2) ◽  
pp. 67-71 ◽  
Author(s):  
P Sabatelli ◽  
S Squarzoni ◽  
S Petrini ◽  
C Capanni ◽  
A Ognibene ◽  
...  
Keyword(s):  
Muscular Dystrophy ◽  
Exfoliative Cytology ◽  
Non Invasive ◽  
Oral Exfoliative Cytology
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