The effectiveness of immunoglobulin G application in patients with drug­-resistant tuberculosis/HIV with CD4+ lymphocytes from 200 to 50 cells/µl according to biochemical data

2021 ◽  
pp. 43-50
Author(s):  
N.А. Matsegora ◽  
A.V. Kaprosh
Keyword(s):  
Immunoglobulin G ◽  
Biochemical Parameters ◽  
Immunoglobulin Therapy ◽  
Treatment Method ◽  
Control Group ◽  
Second Line ◽  
Resistant Tuberculosis ◽  
Complex Therapy ◽  
Group 2 ◽  
Cd4 Lymphocytes

Objective — to study the effectiveness of immunoglobulin therapy in patients with co-infection of drug-resistant tuberculosis (DR-TB)/HIV at the level of CD4+ lymphocytes from 200 to 50 cells/μl, based on a study of the dynamics of biochemical parameters. Materials and methods. The study involved 52 patients aged 20 to 55 years, with a mean age of (37.2 ± 7.8) years. All patients were HIV-positive with laboratory-confirmed DR-TB with mycobacterial resistance to first- and second-line drugs. Patients with DR-TB/HIV were distributed as follows: 1 group (control) — 26 patients with DR-TB/HIV, receiving standard treatment of second-line AMBP and ARVT; group 2 (main) — 26 patients with DR-TB/HIV, who also received standard treat­ment of second-line AMBP and ARVT, with the addition of complex therapy with intravenous immunoglobulin G (IgG). Results and discussion. Against the background of treatment, the dynamics of changes in biochemical parameters was as follows: patients in the control group, after the first 2 weeks of AMBP, there was an increase in intoxication load on the hepatobiliary and urinary systems, which led, on the one hand, to dysfunction liver with hyperbilirubinemia, increased transaminase activity, thymol turbidity, and on the other — to the development of renal failure with hypercreatininemia, hyperuricemia, azotemia and oliguria. As a result, timely appointment of ARVT (2 weeks after AMBP) in 19 (73.1 %) patients was impossible and was carried out much later than desired (after 2—3 months).Treatment of patients with comorbid pathology of DR-TB/HIV, which included complex intravenous IgG on the background of complex therapy, was accompanied by positive clinical and laboratory dynamics, which created conditions for ARVT involvement after the second week of complex therapy according to the developed treatment method. Conclusions. The use of immunologically targeted treatment with intravenous IgG made it possible to successfully prescribe ARVT to patients of the main group in 2 weeks from the start of AMBT, which is very useful to prevent adverse reactions, increase treatment efficacy and reduce mortality in patients with comorbid DR-TB/HIV in a state of deep immunosuppression.

scholarly journals Osteopathic Correction in Complex Therapy of Patients with Mild Cognitive Impairment

2017 ◽  
pp. 40-47
Author(s):  
O. F. Anufriev ◽  
V. O. Belash ◽  
V. M. Utochkin
Keyword(s):  
Cognitive Impairment ◽  
Mild Cognitive Impairment ◽  
Focus Group ◽  
Depression Scale ◽  
Control Group ◽  
Somatic Dysfunction ◽  
Complex Therapy ◽  
Before And After ◽  
Group 2 ◽  
Positive Effect

Goal of research - the study aims to evaluate the effectiveness and scientifi c basis for the use of osteopathic correction in the treatment of cognitive impairment.Materials and methods. Two groups of patients participated in the study: Group 1 (focus group) included 22 patients with a syndrome of mild cognitive impairment who received medication and osteopathic correction; Group 2 (control group) consisted of 22 patients who received medication only. The groups were comparable in age, sex, and severity of cognitive impairment. All patients observed before and after treatment underwent complex osteopathic and neuropsychological examinations.Results. The study found that the inclusion of osteopathic correction in the treatment of patients with mild cognitive impairment has a positive effect on the dynamics of a number of neuropsychological tests. In particular, there is a statistically signifi cant increase in the indicators based on the results of the Montreal Cognitive Evaluation Scale; there is a decrease in the overall anxiety level based on the results of the Hospital Alarm and Depression Scale. Moreover, the focus group shows a statistically signifi cant decrease in the severity of somatic dysfunction in the head and neck area, both structural and visceral components, which was not observed in the control group.Conclusion. The results from the study suggest recommending the inclusion of osteopathic correction in the complex therapy for patients with mild cognitive impairment.

scholarly journals The effectiveness of complex therapy using the injectable form of chondroitin sulfate and sodium hyaluronate with osteoarthritis of the knee joint

2019 ◽  
Vol 91 (5) ◽  
pp. 96-102 ◽  
Author(s):  
E A Belyaeva ◽  
O S Avdeeva
Keyword(s):  
Knee Joint ◽  
Chondroitin Sulfate ◽  
Sodium Hyaluronate ◽  
Control Group ◽  
Osteoarthritis Of The Knee ◽  
Complex Therapy ◽  
Group 3 ◽  
Inflammatory Drugs ◽  
Group 2 ◽  
Group 1

Aim. The study on the effectiveness of complex therapy for osteoarthritis (OA) of the knee joint was conducted in real clinical practice. Materials and methods. The survey involved 125 patients aged fr om 50 to 70 years (25 men and 100 women) with a diagnosis of knee joint OA (the III roentgenologic Kellgren-Lawrence stage).The average age of the patients was 62±3.21, the average duration of the disease - 9.4±2.8 years. Patients were randomly assigned to three groups of 35 people, the control group had 20 patients. Group 1 patients received non - steroidal anti - inflammatory drugs (NSAIDs) + Injectran(Chondroitin sulfate) 200 mg intramuscularly (I.M.) every other day No. 25.In group 2, patients received NSAIDs + Fermatron 1% 2 ml with an interval of 7 days intra - articularly (I.A.) No. 3. In group 3 - NSAIDs + Injectran 200 mg (I.M.) every other day No. 25 + Fermatron 1% 2 ml with an interval of 7 days (I.A.) No. 3. In the control group (20 people), patients received only NSAIDs. Evaluation of the symptoms was carried out using the WOMAC index before the start of thetherapy, after 8 and 12 weeks of treatment. The intensity of pain while walking was estimated on a visual analogue scale. Results. In the groups that received Injectran (I; group 1) or Fermatron (F; group 2), the dynamics of pain while walking reduction was comparable and had slightly more than 30% in both groups, the figures are reliable in comparison withinitial data (p

scholarly journals Evaluation of Oxidative Stress and some Biochemical Parameters in Normal Pregnant Women

2020 ◽  
Vol 8 (1) ◽  
pp. 17-23
Author(s):  
Musher I. Kakey ◽  
Kamaran K. Abdoulrahman ◽  
Gulbahar R. Uthman
Keyword(s):  
Pregnant Women ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
First Trimester ◽  
Control Group ◽  
Sod Activity ◽  
Nonsignificant Difference ◽  
Group 2

Pregnancy is associated with many metabolic changes in normal pregnant woman, this leads to change in physiological, biochemical, and hematological parameters drastically. The test subjects were selected among those attending to Maternity Teaching Hospital in Erbil Governorate between March 2017 and August 2017. Four groups of individuals were included in this study, 230 pregnant women divided into three groups (Group 1 first trimester, Group 2 second trimester, and Group 3 third trimester) and Group 4 contained 90 nonpregnant women as control. Full automatic chemical analyzer (Cobas C311, Germany) was used to determine the biochemical parameters. The EL ×800 Absorbance Microplate Reader from BioTek (USA) instruments is used to measure the activity of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in serum. The results of this study showed a significant increase in serum albumin, glucose, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatinine, urea, cholesterol, low-density lipoprotein, and MDA in pregnant women as compared to the control group, also show significant decrease in high-density lipoprotein (HDL) level, SOD activity, and CAT activity in pregnant women as compared to nonpregnant women, and nonsignificant difference in uric acid, and HDL.

scholarly journals Effect of Vasoactive Intestinal Peptide (VIP) on Cytokine Levels and Haemostatic and Biochemical Parameters in A Rat Endotoxaemic Model

2012 ◽  
Vol 56 (1) ◽  
pp. 63-67
Author(s):  
Ramazan Col ◽  
Zafer Durgun
Keyword(s):  
Vasoactive Intestinal Peptide ◽  
Biochemical Parameters ◽  
Control Group ◽  
E Coli ◽  
Group 4 ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

Abstract The presented study was planned to determine whether vasoactive intestinal peptide (VIP) could prevent cytokine haemostatic, haematological, and biochemical disturbances in LPS-treated rats. Adult male Wistar rats (weight range: 200-250 g) were used. The study included four groups: group 1 served as a control group (C); animals in group 2 were given intravenously 1.6 mg/100 g of LPS (E. coli, serotype 0.111:B4); in group 3, rats were injected intraperitoneally with 25 ng/kg of VIP; in group 4, the same doses of VIP and LPS were injected simultaneously. Blood samples were collected 6 h after treatments. In endotoxaemic rats, platelet count, fibrinogen, and antithrombin levels were decreased, the activated partial thromboplastin time and prothrombin time were prolonged, and leucopoenia, as well as significant changes in differential leukocyte percentage were demonstrated. In addition, LPS caused statistically significant increases in plasma TNF- , IL-6, and IL-10 levels, and AST, ALT, creatinine, cholesterol, triglyceride concentrations. However, it caused a statistically significant decrease in total protein and albumin levels when compared to control group. The results showed that during endotoxaemia, VIP had moderately therapeutic effect as an antiinflammatory agent, suppressing TNF-α and IL-6, and stimulating IL-10; however, it was not effective against the adverse effect of LPS on investigated haematological and biochemical parameters.

scholarly journals Effect of potassium nitrate plus vitamin C in feed of rabbits on the some biochemical parameters

2017 ◽  
Vol 40 (2) ◽  
pp. 135-139
Author(s):  
Saad Thabit Jassim Alrawi
Keyword(s):  
Vitamin C ◽  
Biochemical Parameters ◽  
Potassium Nitrate ◽  
Control Group ◽  
Daily Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
C 50 ◽  
Group 1

     This study is conducted at investigating the effect of potassium nitrate and vitamin C in feed of the rabbits on the some biochemical parameters. Twenty eight adult New-Zealand rabbits were divided randomly into four groups (7 rabbits each), they were fed potassium nitrate and vitamin C for 16 weeks as follow: Group 1 (G1) fed potassium nitrate (168 mg/ kg B.W./ daily), group 2 (G2) fed potassium nitrate (168 mg/ kg B.W./ daily) and vitamin C (50 mg/ kg B.W./ daily), group 3 (G3) fed potassium nitrate (168 mg/ kg B.W./ daily)  and vitamin C (100 mg/ kg B.W./ daily) and group 4 (G4) fed basal ration as control group. Blood were collected from heart at zero, eight, twelve and sixteen weeks. The results showed a significant increase (P<0.05) in cholesterol, triglyceride and blood nitrogen urea in the group that had fed potassium nitrate G1 compared with G4, whereas the groups that were fed vitamin C with potassium nitrate showed a mild decrease compared with group potassium nitrate that had been fed potassium nitrate alone (G1). In conclusion, the feeding rabbits with potassium nitrate caused an increase in cholesterol, triglyceride and blood urea concentration in the serum, whereas the vitamin C ameliorates this effect.

scholarly journals Effects of the Treatment Method of Reproductive Performance in Cows with Retention of Fetal Membranes

2017 ◽  
Vol 38 (333) ◽  
pp. 14-23
Author(s):  
Santa Skuja ◽  
Vita Antāne
Keyword(s):  
Treatment Method ◽  
Subclinical Mastitis ◽  
Feed Consumption ◽  
Fetal Membranes ◽  
Control Group ◽  
Group 4 ◽  
Production Body ◽  
Group 2 ◽  
Ceftiofur Hydrochloride ◽  
And Control

AbstractThe aim of the research was to find out an appropriate method of treatment of retention of fetal membranes (RFM) in cows for conditions of Latvia. Three protocols for the treatment of retained fetal membranes in dairy cows were evaluated in a field trial. Cows that retained the fetal membranes for more than 24 hours were assigned to three treatment groups. Group 1 – control group; group 2 – cows with RFM, which were removed manually, and cows were intrauterine treated with antibiotics; group 3 – cows with RFM, which were not removed, and cows were intrauterine treated with antibiotics, and group 4 – cows with RFM, which were not removed, and cows were not treated. In animals with a decreased feed consumption or milk production, body temperature above 39.5 °C, systemic therapy was applied with ceftiofur hydrochloride or procaine benzilpenicillin. In order to establish subclinical endometritis, all cows were histologically examined on the 42ndday postpartum (PP). Subclinical mastitis was diagnosed both in cows with RFM and control group cows. Results indicated that better reproductive results were in cows of group 2 and group 4. Actually, results indicated that treatment of RFM without intrauterine manipulation and parenteral treatment could be as effective as conventional treatment including RFM detachment and local antibiotic treatment.

scholarly journals Effects of Energy Drink in Combination with Two Different Brands of Alcohol on Some Biochemical Parameters of Male Albino Rats

2020 ◽  
Vol 5 (9) ◽  
pp. 707-710
Author(s):  
Okuda, A. Frank ◽  
Ehiwario, N. Julius ◽  
Oshilim, O. Anthony
Keyword(s):  
Biochemical Parameters ◽  
Energy Drink ◽  
Control Group ◽  
Albino Rats ◽  
Treatment Groups ◽  
Group 4 ◽  
Significant Difference ◽  
Group 2 ◽  
Biochemical Analyses ◽  
Group 1

This study was carried out to ascertain the effect of energy drink and mixture of energy drink with different brands of alcohol on some biochemical parameters. Sixteen (16) male albino rats weighing 120- 160g were divided into four (4) groups. The groups were divided thus: Group 1: Control- feed and water ad libitum, Group 2: 3.75ml/kg Bullet Energy drink only, Group 3: 3.75ml/kg Bullet energy drink + 1ml/kg of 41% alcohol dry gin and Group 4: 3.75ml/kg Bullet energy drink + 1ml/kg of 5.1% alcoholic beer. The experiment lasted for 28 days after which the animals were fasted for 12 hours before they were sacrificed and their blood collected for biochemical analyses. There was a significant increase in body weight of animals in group 2-4 and also the glucose concentration was elevated. The result presented in table 2 showed that there is a statistically significant difference (p<0.05) in the level of ALT and ALP in treatment groups when compared to the control group (1). Also, elevated level of cholesterol and triglycerides were also observed. From this data, we may conclude that there are high risk of constantly taking energy drink alone or in combination with alcohol.

scholarly journals Prevalence and main indicators of the combination of resistant tuberculosis and diabetes mellitus

2021 ◽  
pp. 29-29
Author(s):  
L.D. Todoriko ◽  
I.O. Semianiv
Keyword(s):  
Diabetes Mellitus ◽  
Main Group ◽  
Control Group ◽  
Treatment Rate ◽  
Aged Patients ◽  
Retrospective Assessment ◽  
Resistant Tuberculosis ◽  
Patients With Diabetes ◽  
Complicated Course ◽  
Group 2

Background. Diabetes is the background for the development of tuberculosis (TB) and such comorbidity not only significantly complicates the specific process, but also is one of the main risk factors for TB recurrence. Objective. The aim of the study is a comprehensive retrospective assessment of the combination of TB and diabetes. Materials and methods. Our study is based on an analysis of statistical data obtained from a retrospective study of 762 case histories. Results. Depending on the type of TB case in our patients, we found that in both groups of the study the recurrence of TB prevailed – 49 cases (55.7 %) against 39 cases (44.3 %) of people in the main group; 363 cases (53.9 %) against 311 (46.1 %) in the control group (p<0.05). The rate of successful treatment in group 2 is probably higher than in group 1 (64.7 % vs. 61.4 %). However, a more significant probable difference is characterized by the treatment rate, which in the main group is 27.3 % vs 40.3 % in the control group (almost 2 times). The rate of ineffective treatment, which in patients with comorbidity was 27.3 % (almost every third patient) against 17.6 % in group 2 is also important for scientists and practitioners. Conclusions. The pulmonary TB developed significantly more often in middle-aged patients with type 2 diabetes mellitus with moderate and severe states, the subcompensated form, with a complicated course. In patients with diabetes more often was registered a common tuberculous process in the lungs and in all 100 % of patients with syntropy bacterial excretion was registered.

Effect of recombinant interleukin-10 on some haematological and biochemical parameters in a rat endotoxaemic model

2011 ◽  
Vol 59 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Ramazan Çöl ◽  
Zafer Durgun
Keyword(s):  
Biochemical Parameters ◽  
Interleukin 1 ◽  
Interleukin 10 ◽  
Saline Group ◽  
Control Group ◽  
Experimental Sepsis ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Necrosis Factor

Recombinant interleukin-10 (rIL10) has been found to suppress the synthesis of tumour necrosis factor (TNF), interleukin-1 (IL-1), interleukin-6 (IL-6) and tissue factor and to improve survival from experimental sepsis. The aim of this study was to evaluate the protective effect of rIL-10 on lipopolysaccharide-(LPS-) induced haematological and biochemical disturbances in rats. In the present study, 40 rats were used and divided equally into four groups. Group 1 (control group, C) was treated with 0.9% saline. Group 2: LPS was injected intravenously (1.6 mg/100 g), Group 3 received rIL10 treatment (125 μg/kg) 2 min before 0.9% saline injection, Group 4 received rIL10 treatment 2 min before endotoxin treatment. When compared with the controls, platelet count, leukocyte count (with a marked neutrophilia and lymphopenia) and fibrinogen were decreased, while activated partial thromboplastin time (APTT) and prothrombin time (PT) were prolonged in the endotoxaemic rats. In addition, LPS caused statistically significant increases in plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities as well as creatinine, cholesterol and triglyceride concentrations, while it caused a statistically significant decrease in glucose, total protein and albumin levels as compared to the control group. On the other hand, rIL10 significantly suppressed disturbances in the haematological and biochemical parameters associated with endotoxaemia. As a result, rIL10 may be efficacious in preventing haematological disorders, tissue damage and changes in lipid, protein and carbohydrate metabolism in endotoxaemia.

scholarly journals Effects of Dietary FOS and CPP on growth performance and serum biochemical parameters for weaned piglets

2019 ◽  
Vol 131 ◽  
pp. 01078
Author(s):  
Feng Zhao ◽  
Zhongsheng Xia
Keyword(s):  
Growth Performance ◽  
Biochemical Parameters ◽  
Control Group ◽  
Female Group ◽  
Apparent Digestibility ◽  
Serum Biochemical Parameters ◽  
Weaned Piglets ◽  
Significant Difference ◽  
Serum Biochemical ◽  
Group 2

The present experiment was conducted to study the effects of different levels of oligosaccharide(FOS) and complex CPP preparation(CPP) in diets on growth performance and serum biochemical parameters for weaned piglets. 240 crossbred DLY( Duroc× Landrace× Yorkshire) 21-day-old weaning piglets, about 7.2 kg in initial body weight were selected and randomly divided into 6 groups, with 2 repeats for each group, 20 each repeat(half male and half female.). Group 1, as the control group, was fed base diet(BD), group 2 was fed BD adding 0.10% aureomycin, group 3, 4, 5, 6, respectively, was fed BD adding 0.05% CPP, 0.05% FOS, 0.20% FOS and 0.10% FOS + 0.05% of CPP. Feeding trial was conducted for 35 days. Results: (1) The combination of 0.10%FOS and 0.05%CPP(group 6) were added, the average daily gain of weaned pigs increased by 13.71 % (P < 0.05) and the feed conversion ratio was reduced by 7.41 % (P < 0.05) compared with control. The diarrhea rate of piglets decreased by 94.63 % (P < 0.01) compared with the control (p < 0.05), and decreased by 62.86 % (P <0.05) compared with group 2.(2)There was no significant difference in the apparent digestibility of nutrient, such as ether extract and crude protein, by adding FOS and CPP individually or jointly in diet of weaned piglets, but apparent digestibility of crude fiber increased , to acertain extent, and there was no significant difference with diet adding 0.10 % of antibiotics (group 2)(P >0.05).(3) There was no significant difference in serum biochemical indexes, such as serum total protein, albumin, urea nitrogen, cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase and sugar by adding FOS and CPP individually or jointly in diet of weaned piglets (P > 0.05);there was no significant effect in serum immunoglobulin IgG and IgM (P> 0.05), but the serum IgE content was increased in different degrees. Conclusion: Adding FOS and CPP in diet for weaned piglets can improve the growth performance, reduce diarrhea rate of piglets, and promote digestion and absorption of feed nutrients, without significant difference between them and diet adding antibiotics. Among them, the effect of adding 0.10%FOS+0.05%CPP was the best.

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