scholarly journals Development of new antigens for serologic diagnosis of animal trypanosomosis and their use for epizootic monitoring

2021 ◽  
Vol 15 (1) ◽  
pp. 71-78
Author(s):  
Ch. Georgiou
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Test System ◽  
Fixation Test ◽  
Federal State ◽  
State Budget ◽  
Serological Tests ◽  
Hemagglutination Test ◽  
Indirect Hemagglutination ◽  
Indirect Hemagglutination Test

The purpose of the research is developing a method for obtaining erythrocyte antigens containing and not containing Trypanosoma equiperdum and T. evansi DNA, which can later be used in serological reactions to differentiate these types of Trypanosoma.Materials and methods. The studies were conducted in the Protozoology Laboratory and the Vyshnevolotsk Branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV RAS”, as well as livestock farms of the Russian Federation and other countries using clinical, microscopic, hematological, parasitological, biomolecular and serological methods.Results and discussion. Studies carried out for the first time have shown that it is possible to use erythrocyte antigens containing the T. equiperdum and T. evansi DNA obtained after 3-fold administration to mice and rabbits of a mixture of trypanosomal antigen with addition of 1.0 ml of an adjuvant (aluminum hydroxide), and bleeding of animals at 25 to 30 days. The formed precipitate was used as an antigen for serological tests. Experiments have shown that blood for preparation of positive serum can be taken when antibodies are in titers of 1:20 in the Prolonged Complement Fixation Test, and at least 1:400 in the Indirect Hemagglutination Test and ELISA, and for negative serum when horse blood serum reacts negatively with antigens of T. equiperdum and T. evansi in the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA. The test systems of the Prolonged Complement Fixation Test, Indirect Hemagglutination Test and ELISA prepared by us with antigens containing and not containing T. equiperdum and T. evansi DNA resulted in creating a universal test system (Indirect Hemagglutination Test) for differentiating T. equiperdum from T. evansi.

scholarly journals Model Proficiency Testing Scheme for Serological Diagnosis of Brucellosis: Interlaboratory Study

2004 ◽  
Vol 87 (4) ◽  
pp. 965-971 ◽  
Author(s):  
Donatella Nannini ◽  
Manuela Tittarelli ◽  
Lucilla Ricci ◽  
Annamaria Conte ◽  
Bernardo Di Emidio ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reference Laboratory ◽  
Correct Result ◽  
The European Union ◽  
Serological Tests ◽  
Testing Scheme ◽  
Z Scores ◽  
Qualitative Test

Abstract A model interlaboratory testing scheme was developed by the Italian National Reference Laboratory for Brucellosis. This scheme was planned for both qualitative (Rose Bengal Plate Test; RBPT) and quantitative (Complement Fixation Test; CFT) serological tests and involved a total of 42 laboratories. In the preparation of this scheme, reference was made to general protocols and guidelines and to methods reported in the literature, which were applicable to analytical chemistry laboratories. Six field sera from naturally infected animals, one positive serum at a titer below the European Union (EU) positivity threshold, and 5 sera positive at titers between 20 and 851 International Units of Complement Fixation Test (IUCFT)/mL plus one negative serum were used to produce a panel of test sera. To evaluate laboratory performances in the quantitative test for each tested sample examined, z-scores based on robust summary statistics (the median and normalized interquartile range) were used. To evaluate overall laboratory performance, 2 types of combined z-scores were used: Rescaled Sum of Scores and Sum of Squared Scores. In the case of the qualitative test (RBPT), results were analyzed by a Bayesian approach. A Beta distribution, based on the result of each laboratory, was calculated and used to estimate the probability of each laboratory giving a correct result and its uncertainty.

scholarly journals An epidemic ofMycoplasma pneumoniaein Manitoba: A Serological Report

1993 ◽  
Vol 4 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Laila Sekla ◽  
Walter Stackiw ◽  
Gudrun Eibisch ◽  
Donna Kolton
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Respiratory Infections ◽  
Antibody Test ◽  
Fixation Test ◽  
Age Group ◽  
Serological Tests ◽  
Igm Antibodies ◽  
Indirect Immunofluorescent ◽  
Immunofluorescent Antibody Test

Objectives: To report an epidemic ofMycoplasma pneumoniaein Manitoba and to discuss the limitations of the serodiagnostic tests used.Design: A retrospective analysis of the results of a province-wide serological testing for respiratory infections caused byM pneumoniae,using a complement fixation test and an indirect immunofluorescent antibody test for the detection of immunoglobulin (Ig) M antibodies.Material: From April 1, 1987, to March 31, 1991, 12,804 sera were tested and a serological diagnosis of recentM pneumoniaeinfections were established in 509 (3.97%). From April 1 to September 30, 1991, an additional 2088 persons were tested; the 158 (7.5%) recent cases ofM pneumoniaewere subjected to analysis.Results: Compared with the previous three years, an increase in the number of recent cases ofM pneumoniaewas first noticed in July 1990 which persisted until September 1991. Of 856 single sera tested, 59 (6.8%) were recentM pneumoniaeinfections and 56 (96.1%) of these were positive for IgM antibodies. Of the 616 persons who submitted paired sera, 99 (16%) were recent infections, but only 46 (46.4%) had IgM antibodies. Primary infections (ie, positive for IgM antibodies) were detected in 102 (64.5%) and reinfections (ie, positive complement fixation test only) in the remaining 56 persons with recentM pneumoniaeinfections. Primary infections were detected more frequently in the ‘under 16’ than in the ‘over 16’ year age group (75% versus 55.8% of the recent cases ofM pneumoniaein each age group). Reinfections were more common in the older age group. Of the 158 recent cases ofM pneumoniae,30.3% had a pneumonia; of these, 21 (55.2%) were under the age of 16 years.Discussion:M pneumoniaeis an important cause of morbidity. Serological tests are used for the diagnosis despite their limitations. The detection of IgM antibodies in acute serum establishes a diagnosis of primaryM pneumoniae;however, their absence does not excludeM pneumoniae. Asecond (convalescent) blood test is required to diagnose all primary infections. To diagnose all reinfections, paired sera should be tested by complement fixation.Summary: Manitoba experienced an epidemic ofM pneumoniaein 1990–91. Properly selected serological tests can provide a specific and rapid diagnosis.

scholarly journals Molluscum contagiosum: serology and electron microscopy findings in twenty one patients

1987 ◽  
Vol 29 (2) ◽  
pp. 86-89 ◽  
Author(s):  
M. E. F. Fonseca ◽  
R. D. Machado ◽  
M. I. M. Liberto ◽  
G. Marcolino
Keyword(s):  
Electron Microscopy ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Virus Disease ◽  
Molluscum Contagiosum ◽  
Fixation Test ◽  
Molluscum Contagiosum Virus ◽  
Serological Tests ◽  
Specific Antibodies ◽  
Electron Microscopical

Twenty one cases of molluscum contagiosum virus disease were collected for electron microscopical and serological tests. Molluscum virus was detected in the crust, inside the vacuoles formed in the keratinocytes cells. The patients developed specific antibodies to the virus detected by complement fixation test.

INDIRECT FLUORESCENT ANTIBODY METHOD IN SERODIAGNOSIS OF TOXOPLASMOSIS

1962 ◽  
Vol 8 (4) ◽  
pp. 545-554 ◽  
Author(s):  
A. E. Kelen ◽  
L. Ayllon-Leindl ◽  
N. A. Labzoffsky
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Fixation Test ◽  
The Other ◽  
Staining Procedure ◽  
Indirect Fluorescent Antibody ◽  
Serological Tests

The principle of the indirect fluorescent antibody-staining procedure was adapted for use in the serodiagnosis of toxoplasmosis and the technique compared with other serological tests most commonly employed at present.The results obtained with normal and immune rabbit sera as well as human patients' sera indicate that the indirect fluorescent antibody test for toxoplasmosis is specific in a degree comparable to the complement fixation test, and is sensitive enough for routine laboratory use, its sensitivity being somewhat higher than that of the complement fixation test. The Sabin–Feldman dye test gave positive results with human sera from cases clinically unrelated to toxoplasmosis much more frequently and usually in higher titers than did the complement fixation and the fluorescent antibody tests. On the other hand, the dye test showed a few negative results on sera for which the other tests proved to be positive. The indirect fluorescent antibody test for toxoplasmosis is safer and simpler to perform than the dye test, as living organisms are not used in the test, and prepared smears of killed toxoplasma suspension on cover slips can be kept antigenically active in the frozen state for at least 6 months.The indirect fluorescent antibody test for toxoplasmosis is recommended for use in the serodiagnosis of toxoplasmosis either as a single test or as a supplementary test for checking complement fixation negative sera or the results obtained by the dye test.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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