scholarly journals ON THE DOG BABESIASIS AND MEASURES OF ITS PREVENTION AND TREATMENT

2020 ◽  
pp. 234-239
Author(s):  
Movsesyan ◽  
Petrosyan ◽  
Vardanyan ◽  
Nykogosyan ◽  
Manukyan
Keyword(s):  
Clinical Symptoms ◽  
Rhipicephalus Sanguineus ◽  
Ixodid Ticks ◽  
Smear Microscopy ◽  
Babesia Canis ◽  
Intensity Of Infection ◽  
Hunting Dogs ◽  
Blood Smears ◽  
Forest Mountain ◽  
Peripheral Blood Smears

A haemoparasitic disease, babesiasis was studied in dogs, brought to the Laura Dog Club, located in Yerevan city. Work in this direction was conducted from midApril till the end of November 2019. 25 working and hunting dogs, and 5 stray dogs were examined. Peripheral blood smears were prepared from animals with severe clinical symptoms of haemoparasitic disease to differentiate babesiasis. After fixing and staining, smear microscopy was performed under an immersion microscope system. Studies of blood smears showed that Babesia canis was found in red blood cells of examined dogs. The carriers of Babesia canis are Ixodid tics of the species Rhipicephalus sanguineus, Rh. bursa, Dermacentor marginatus, the latter species was found in a dog brought from the territory of the forest-mountain zone of Armenia. The species of Ixodid ticks, and the degree of tick infestation, were determined. It turned out that the dogs were mainly infected by the species Rh. sanguineus. It was found that the intensity of infection by Ixodid ticks in dogs in summer was 100%, and the intensity of infection was 10–20 ticks in each animal. Almost the same picture of ticking was observed in autumn.

scholarly journals Antioxidant status, and blood zinc and copper concentrations in dogs with uncomplicated babesiosis due to Babesia canis infections

2021 ◽  
Vol 65 (2) ◽  
pp. 169-174
Author(s):  
Oliwier Teodorowski ◽  
Stanisław Winiarczyk ◽  
Duygu Tarhan ◽  
Banu Dokuzeylül ◽  
Alev Meltem Ercan ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Antioxidant Defence ◽  
Blood Concentrations ◽  
Naturally Occurring ◽  
Blood Smears ◽  
Zinc Concentrations ◽  
Healthy Dogs ◽  
Peripheral Blood Smears ◽  
Blood Zinc ◽  
Apparently Healthy

Abstract Introduction The aim of the study was to demonstrate a link between uncomplicated Babesia canis infection in dogs and blood concentrations of zinc and copper and erythrocytic antioxidant defence – activities of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Material and Methods The study was based on 15 naturally occurring cases of canine babesiosis with anorexia, pyrexia, depression, pale mucous membrane, splenomegaly and dark red urine. Microscopic examination of Giemsa-stained peripheral blood smears and the results of PCR confirmed B. canis infection. Seven apparently healthy dogs brought in for either a check-up or vaccination were used for comparison. Results The levels of the erythrocytic antioxidant enzymes - SOD and CAT - were significantly higher in the infected dogs than in cytologically negative dogs. The levels of blood micronutrients were significantly lower in the infected dogs (0.478 μg of zinc per mL vs 1.241 μg/mL and 0.722 μg of copper per mL vs 1.392 μg/mL). Conclusion Oxidative stress can be posited as one of the mechanisms leading to anaemia in dogs with babesiosis, and therefore antioxidant biomarker and copper and zinc concentrations could be used as indicators of disease severity and prognostic markers.

scholarly journals Епізоотологічний моніторинг бабезійної інвазії серед собак у Полтаві

2017 ◽  
Vol 19 (73) ◽  
pp. 149-153
Author(s):  
Yu.О. Моkryi ◽  
І.М. Кsyonz
Keyword(s):  
Veterinary Medicine ◽  
Blood Smear ◽  
Preventive Measures ◽  
Clinical Symptoms ◽  
Babesia Canis ◽  
Scientific Interest ◽  
Blood Smears ◽  
Study Results ◽  
Causative Agents ◽  
Dog Blood

Babesiosis of dogs is a blood infestation disease caused by protozoa of Babesia genus. There are several causative agents of the disease, but in most cases, the etiological factor of dogs’ disease is Babesia canis. The disease is transmitted by ticks of Dermacentor and Ixodes genera. Pathogen is transmitted from the tick to the dog in the process of the tick’s eating the dog’s blood. The aim of the research was to study babesiosis epizootology of dogs in Poltava. As a result of epizootology monitoring of babesiosis for the 9-years period (2007 to 2016), seasonality was established, gender, age and pedigree dependency of incidence in dogs was defined. Statistic data of Poltava veterinary medicine institutions served as the study material. Diagnosis of babesiosis was determined based on the disease history, peculiar clinical symptoms and results of microscopic detection of Babesia in blood smears prepared from peripheral blood (ear vein) and Romanowsky-Hiemsa stained. Dog blood smear study was performed under the immersion system of 90 × 10 power light microscope. For the above mentioned period, 7866 cases of babesiosis in dogs have been recorded. Analysis of the data obtained shows that the said infestation has been recorded throughout the year, with winter cases being sporadic and their significant reduction being observed in hot summer months. In general, the distinct seasonal dynamics was defined with the two peak periods: April and September-October. Among the diseased dogs, males overwhelm females by 20%. The most susceptible to the disease are dogs aged from 2 to 3 years (11.1–16%), less – animals of 5–8 years of age (6.2–7.5%) and the least number of cases is recorded in dogs from 8 to 18 years of age (0.1–4.4%), i.e. a steady tendency is traced of susceptibility to babesiosis reducing with age. Concerning the breed ratio, the most susceptible are mongrel dogs (20.9%), German Shepherds (13.9%) and Rottweilers (7.5%). The prospect of further research is creation of the preventive measures strategy to minimize the incidence of babesiosis in dogs, on the basis of the obtained epizootic monitoring data. Finally, the 9-years study results present a scientific interest for parasitologists in human and veterinary medicine.

Investigation of Tickborne Pathogens within Naturally Infected Brown Dog Tick (Ixodidae: Rhipicephalus Sanguineus) in Egypt by Light and Electron Microscopy

2020 ◽  
Keyword(s):  
Electron Microscopy ◽  
Salivary Gland ◽  
Light And Electron Microscopy ◽  
Rhipicephalus Sanguineus ◽  
Mammalian Host ◽  
Babesia Canis ◽  
Brown Dog Tick ◽  
Transmission Electron ◽  
Dog Tick ◽  
Theileria Spp

Tick borne pathogens present a significant health challenge to animals and human because a single tick may transmit multiple pathogens to a mammalian host during feeding. The present study detected tick-borne pathogens from pet dogs. A total of 666 ticks were collected from 144 pet and sheltered dogs in Egypt from April to September 2018. For hemolymph, midgut and salivary gland smears 546 ticks were used as well as 360 egg smears from 120 female tick were examined by light microscope. The infected ticks were prepared for transmission electron microscopy (TEM). Ticks were identified; Rhipicephalus sanguineus. Light microscopy showed infection rates of 44.69%, 68.50% & 15.75%, in hemolymph, midgut and salivary gland, respectively. H. canis recorded the highest rates in hemolymph and midgut (35.89% & 49.82%, respectively), but Theileria spp. was the lowest (0.73% & 2.93%, respectively). In salivary gland smears, Babesia canis. was detected in 13.55% and Theileria spp. in 1.83%. Mixed infection in same tick was recorded in 4.76% &0.37% in midgut and salivary gland smears, respectively. Babesia canis stages were recovered from 15% of egg smears. R. sanguineus was natural infected by Babesia, Theileria, Hepatozoon and Anaplasma phagocytophilum as well as mixed infections of protozoa accompanied by a complicated sign of diseases and failure in accurate diagnosis.

Brightfield and fluorescence in-channel staining of thin blood smears generated in a pumpless microfluidic

2021 ◽  
Author(s):  
Kokou S. Dogbevi ◽  
Paul Gordon ◽  
Kimberly L. Branan ◽  
Bryan Khai D. Ngo ◽  
Kevin B. Kiefer ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Thin Blood Smears

Effective staining of peripheral blood smears which enhances the contrast of intracellular components and biomarkers is essential for the accurate characterization, diagnosis, and monitoring of various diseases such as malaria.

scholarly journals Histoplasma capsulatum in peripheral blood smears

2002 ◽  
Vol 116 (3) ◽  
pp. 503-503 ◽  
Author(s):  
Glen A. Kennedy ◽  
Jennifer L. Curnow ◽  
Julie Gooch ◽  
Bronwyn Williams ◽  
Peter Wood ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Histoplasma Capsulatum ◽  
Blood Smears ◽  
Peripheral Blood Smears

Performance of the CellaVision DC-1 digital cell imaging analyser for differential counting and morphological classification of blood cell

2021 ◽  
pp. jclinpath-2021-207863
Author(s):  
Lisa N van der Vorm ◽  
Henriët A Hendriks ◽  
Simone M Smits
Keyword(s):  
Blood Cell ◽  
Peripheral Blood ◽  
Cell Imaging ◽  
Small Satellite ◽  
Morphological Classification ◽  
Clinical Sensitivity ◽  
Blood Smears ◽  
Low Volume ◽  
Peripheral Blood Smears

AimsRecently, a new automated digital cell imaging analyser (Sysmex CellaVision DC-1), intended for use in low-volume and small satellite laboratories, has become available. The purpose of this study was to compare the performance of the DC-1 with the Sysmex DI-60 system and the gold standard, manual microscopy.MethodsWhite blood cell (WBC) differential counts in 100 normal and 100 abnormal peripheral blood smears were compared between the DC-1, the DI-60 and manual microscopy to establish accuracy, within-run imprecision, clinical sensitivity and specificity. Moreover, the agreement between precharacterisation and postcharacterisation of red blood cell (RBC) morphological abnormalities was determined for the DC-1.ResultsWBC preclassification and postclassification results of the DC-1 showed good correlation compared with DI-60 results and manual microscopy. In addition, the within-run SD of the DC-1 was below 1 for all five major WBC classes, indicating good reproducibility. Clinical sensitivity and specificity were, respectively, 96.7%/95.9% compared with the DI-60% and 96.6%/95.3% compared with manual microscopy. The overall agreement on RBC morphology between the precharacterisation and postcharacterisation results ranged from 49% (poikilocytosis) to 100% (hypochromasia, microcytosis and macrocytosis).ConclusionsThe DC-1 has proven to be an accurate digital cell imaging system for differential counting and morphological classification of WBCs and RBCs in peripheral blood smears. It is a compact and easily operated instrument that can offer low-volume and small satellite laboratories the possibilities of readily available blood cell analysis that can be stored and retrieved for consultation with remote locations.

scholarly journals Cytotoxic and Genotoxic Evaluation of the Aqueous and Hydroalcoholic Leaf and Bark Extracts of Crataegus oxyacantha in Murine Model

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2217
Author(s):  
Fany Renata Aguilera-Rodríguez ◽  
Ana Lourdes Zamora-Perez ◽  
Clara Luz Galván-Moreno ◽  
Rosalinda Gutiérrez-Hernández ◽  
Claudia Araceli Reyes Estrada ◽  
...  
Keyword(s):  
Murine Model ◽  
Leaf Extract ◽  
Micronucleus Test ◽  
Genotoxic Effects ◽  
Cytotoxic Effects ◽  
Blood Smears ◽  
Polychromatic Erythrocytes ◽  
Aqueous Leaf Extract ◽  
Peripheral Blood Smears ◽  
Sampling Times

Crataegus oxyacantha has been mainly used in traditional medicine for the treatment of cardiovascular diseases. However, its safety profile has not been fully established, since only the genotoxic effects of C. oxyacantha fruit have been described. Therefore, the objective of this work was evaluating the cytotoxicity and genotoxicity of the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha by means of the micronucleus test in a murine model. Doses of 2000, 1000, and 500 mg/kg of both extracts were administered orally for 5 days in mice of the Balb-C strain. Peripheral blood smears were performed at 0, 24, 48, 72, and 96 h after each administration. The number of polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MNPCEs), and micronucleated erythrocytes (MNEs) was determined at the different sampling times. Our results showed that the leaf and bark of C. oxyacantha increase the number of MNEs at the 2000 mg/kg dose, and only the aqueous leaf extract decreases the number of PCEs at the same dose. Therefore, the aqueous and hydroalcoholic leaf and bark extracts of C. oxyacantha showed genotoxic effects, and only the aqueous leaf extract exhibited cytotoxic effects.

scholarly journals Diagnostic and prognostic significance of Sezary cells in peripheral blood smears from patients with cutaneous T cell lymphoma

Blood ◽  
1985 ◽  
Vol 66 (2) ◽  
pp. 358-366
Author(s):  
EC Vonderheid ◽  
EL Sobel ◽  
PC Nowell ◽  
JB Finan ◽  
MK Helfrich ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Large Diameter ◽  
Prognostic Significance ◽  
T Cell Lymphoma ◽  
Cell Diameter ◽  
Cutaneous T Cell Lymphoma ◽  
Blood Smears ◽  
Duration Of Disease ◽  
Peripheral Blood Smears

Blood smears stained with Wright-Giemsa were obtained from 124 patients with pathologically confirmed cutaneous T cell lymphoma (CTCL), 70 patients with various other cutaneous disorders, and ten healthy adult volunteers. These were examined in a blinded fashion for atypical lymphocytes with cerebriform nuclei (CLs), which were characterized further according to cell diameter. CLs, comprising up to 15% of lymphocytes in smears, were observed in 20% of the patients with benign dermatitis. CLs, comprising up to 89% of lymphocytes in smears, were found in 22%, 30%, 50%, and 96% of patients with patch, plaque, tumor, and erythrodermic CTCL, respectively. Large-diameter CLs (15 to 20 micron) were observed only in smears from patients with CTCL. Total CL counts above 15 per 100 lymphocytes and/or the presence of large CLs occurred in 33 of 49 (67%) patients with erythrodermic disease and in only two patients with other skin manifestations. Blood smears obtained at the time of cytogenetic studies indicated that a total CL count above 15% was the smear criterion that correlated best with the demonstration of a chromosomally abnormal malignant clone in the blood. The presence of large CLs per se, although also predictive of a malignant clone, was less useful. Multivariate survival analysis showed that the duration of disease before the blood smear and the proportion of large CLs within the total CL population were the covariates that correlated most significantly with survival. We speculate that the reduced survival of patients with increased proportions of large CLs in smears reflects the presence of polyploid malignant lymphocytes in the blood.

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