scholarly journals Effect of harmones on callus induction in Maize (Zea mays L.)

2018 ◽  
Vol 10 (1) ◽  
pp. 202-209
Author(s):  
N. Malini ◽  
C. R. Anandakumar ◽  
R. Gnanam ◽  
S. Hari Ramakrishnan
Keyword(s):  
Zea Mays ◽  
Callus Induction ◽  
Zea Mays L ◽  
Callus Formation ◽  
Immature Embryo ◽  
Medium Composition ◽  
Root Tips ◽  
Maize Varieties ◽  
Immature Embryo Culture ◽  
Critical Process

Callus induction from explants is a critical process in regeneration, micropropagation and transformation of maize (Zea mays L.) plants. Formation of callus from plant tissues on culture is affected by several factors. This study revealed to establish the effect of genotype, source of explants and auxin concentration on callus induction from five genotypes UMI 757 (G1), UMI 615 (G2), UMI 112 (G3), UMI 285 (G4) and CO 1 (G5) and one hybrid CO H (M) 5 (G6). Callus induction of the six maize varieties was investigated using immature embryos (E1), leaf bits (E2), root tips (E3), hypocotyls (E4) and seeds (E5) as explants with different concentrations of hormones. In this study, immature embryo was taken from 10 to 12 days after pollination (DAP) to get maximum response. The highest percentage of callus induction was observed (99.10) in immature embryo culture and seed culture gave the highest percentage of rhizogenic callus formation when compare to immature embryo. Among the genotypes tested, CO H (M) 5 recorded the highest callus induction percentage on (2D2K2) medium composition.

scholarly journals Identification of Associations between SSR Markers and Quantitative Traits of Maize (Zea mays L.)

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 182
Author(s):  
Jan Bocianowski ◽  
Kamila Nowosad ◽  
Barbara Wróbel ◽  
Piotr Szulc
Keyword(s):  
Zea Mays ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Quantitative Traits ◽  
Zea Mays L ◽  
Fresh Weight ◽  
Multiple Traits ◽  
Good Tool ◽  
Maize Varieties ◽  
Hybrid Maize

Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. Marker-assisted selection is an important tool for plant breeders to increase the efficiency of a breeding process, especially for multigenic traits, highly influenced by the environment. In this paper, the relationships between SSR markers and 26 quantitative traits of hybrid maize varieties (Zea mays L.) were analyzed. Association analyses were performed based on 30 SSR primers in a set of thirteen hybrid maize varieties. A total of 112 SSR markers were detected in these genotypes. The number of alleles per locus ranged from 1 to 17, with the average number of alleles per locus equal to 3.7. The number of molecular markers associated with observed traits ranged from 1 (for the number of kernels in row, ears weight and fresh weight of one plant) to 14 (for damage of maize caused by P. nubilalis) in 2016 as well as from 1 (for soil plant analysis development—SPAD, the number of grains in ear and fresh weight of one plant) to 12 (for carotenoids content) in 2017. The sum of statistically significant associations between SSR markers and at least one trait was equal to one hundred sixty in 2016 as well as one hundred twenty-five in 2017. Marker trait associations (MTAs) were found on the basis of regression analysis. The proportion of the total phenotypic variances of individual traits explained by the marker ranged from 24.4% to 77.7% in the first year of study and from 24.3% to 77.9% in 2017. Twenty-two SSR markers performed a significant effect on at least one tested trait in both years of experiment. The three markers (phi021/4, phi036/3, and phi061/2) can be a good tool in marker-assisted selection because they allow simultaneous selection for multiple traits in both years of study, such as the number of kernels in row and the number of grains in ear (phi021/4), the number of plant after germination, the number of plants before harvest, and the number of ears (phi036/3), as well as moisture of grain and length of ears (phi061/2).

scholarly journals Aflatoxigenic contamination of freshly harvested white maize (Zea mays L.) from some selected Ugandan districts

2019 ◽  
Author(s):  
Timothy Omara
Keyword(s):  
Zea Mays ◽  
Moisture Content ◽  
Ochratoxin A ◽  
Zea Mays L ◽  
Lake Victoria ◽  
Total Aflatoxin ◽  
Strategic Adaptation ◽  
Maize Varieties ◽  
Agroecological Zones

The moisture content and total aflatoxin (AF) content of 27 samples of freshly harvested white maize (Zea mays L.) from Mubende (n = 3), Ibanda (n = 3), Jinja (n = 3), Mayuge (n = 3) , Buikwe (n = 3), Hoima (n = 3), Mpigi (n = 3), Masindi (n = 3) and Bugiri (n = 3) districts of Uganda representing the agroecological zones: Lake Victoria crescent, Western Highlands, South East and Lake Albert Crescent were determined in the second season harvest of January 2019 to March 2019. Moisture content ranged from 12.9 to 18.8% (mean moisture content varied from 13.9±0.35-17.2±1.55%) with the highest moisture recorded in maize from Ibanda. The highest mean AF contamination of 11.0±3.01 μg/kg was recorded in maize from Hoima while the lowest AF content of 3.8±1.30 μg/kg was recorded in maize from Mpigi. Despite the fact that all the samples had detectable aflatoxins, none of the maize samples had aflatoxin greater than WHO regulatory limit of 20 μg/kg. White maize in Uganda are precontaminated by aflatoxins prior to harvest. Whereas the spectre of aflatoxigenic contamination of foods remains a ticklish challenge to address, strategic adaptation and deployment of appropriate interventions can help secure a safe harvest. Farmers should plant maize varieties with established maturity periods to ensure timely harvesting. Further research should assess the presence of other mycotoxins as zearalenone, sterigmatocystin, ochratoxin A, citrinin, vomitoxin and diacetoxyscirpenol that may co-occur with aflatoxins in freshly harvested maize.

scholarly journals In vitro Callus Induction and Regeneration of Popular Indica Rice Genotypes

2020 ◽  
Vol 13 (4) ◽  
Author(s):  
Aananthi. N
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Immature Embryo ◽  
Coconut Milk ◽  
Rice Genotypes ◽  
Mist Chamber ◽  
Regenerated Plant ◽  
Better Than

Five rice cultivars viz., ASD 16, White Ponni, Pusa Basmati 1, Pusa Sugandh 4 and Pusa Sugandh 5 belonging to subspecies indica were compared for its ability in callus formation and regeneration. In this experiment, the different parameters viz., the effect of hormones (2,4-D and kinetin), organic supplement (coconut milk O1-CM 100 mll-1, O2-CM 75 mll-1, O3-CM 50 mll-1), explants (seed and immature embryo), media (MS and N6), carbon source (sucrose and maltose) using five genotypes on callus response was studied. The effect of hardening methods was also assessed. Results showed that for enhanced callus induction was with MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltose irrespective of explants used. Addition of 100 ml l-1 coconut milk was found have improvement in callus response. The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration. MS media provided superiority over N6. Among the genotypes Pusa Basmati 1 rendered outstanding performance in callus behavior. The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets. Hardening in mist chamber was recognized as the best method to give the highest per cent of regenerated plant lets.

Callus induction from maize (Zea mays L.) mature embryos

2008 ◽  
Vol 136 ◽  
pp. S169
Author(s):  
Xiudong Gao ◽  
Hua Jin ◽  
Young-Boum Shin ◽  
Soon-Kwan Hong
Keyword(s):  
Zea Mays ◽  
Callus Induction ◽  
Zea Mays L ◽  
Mature Embryos

scholarly journals Effect Of Grant Palm Oil Ash To The Growth And Production Of Two Corn Varieties (Zea Mays L) In Peatland

2020 ◽  
Author(s):  
Marlina
Keyword(s):  
Zea Mays ◽  
Palm Oil ◽  
Zea Mays L ◽  
Soil Analysis ◽  
Block Design ◽  
Peat Soil ◽  
Randomized Block Design ◽  
Maize Varieties ◽  
Two Factors

The study, entitled the effect of the administration of Palm Oil Long Palm (AJKS) to the growth and production of two corn varieties inpeatlands, was carried out from October 2017 to March 2018 in trench 3 in Tembilahan Hulu Village, Tembilahan Hulu District, Indragiri Hilir Regency,soil analysis and AJKS conducted at the Bogor Institute of Agriculture Laboratory. The purpose of this study was to determine the effect of AJKSadministration on the growth and production of two maize varieties (Zea mays L) on peatlands, and to determine whether there was an interactionbetween the dosage of AJKS and corn varieties on peat soil ameliorating AJKS. The design used was factorial Randomized Block Design (RBD) using 2factors with three replications. The first factor is the dose of AJKS administration consisting of 4 levels, namely: A0: 0 kg / ha, A1: 500 kg / ha, A2: 1000kg / ha, A3: 1500 kg / ha. The second factor is corn varieties with 2 levels, namely: V1: Pioneer Varieties and V2: Varieties N35. Of the two factors aboveobtained 8 treatment combinations. Based on the results of research that has been done, that there is no real interaction and influence between thetreatment of giving AJKS with varieties to all observed parameters. Giving AJKS 1500 kg / ha gives the highest yields on all parameters observed exceptfor the number of cobs, Pioneer variety is the variety that responds most to AJKS administration.

scholarly journals Emergence of improved varieties of maize (Zea mays L.) as affected by different soil types and planting depths in Chitwan, Nepal

2013 ◽  
Vol 1 ◽  
pp. 23-27
Author(s):  
MN Paudel
Keyword(s):  
Zea Mays ◽  
Seed Quality ◽  
Zea Mays L ◽  
Sandy Loam ◽  
Seed Vigor ◽  
Soil Types ◽  
Red Clay ◽  
Maize Varieties ◽  
Emergence Percentage ◽  
Population Maintenance

Improved open pollinated maize (Zea mays L.) varieties exhibit different emergence reaction as a result of either inferior seed quality or affected by agronomic practices of maize planting. To address problem of low emergence percentage of maize varieties, an experiment was laid out in factorial design under two types of soil (red clay and sandy loam), three level of depth of sowing (7, 14 and 21 cm) for Rampur Composite, Manakamana-1 and Arun-2 maize varieties in Rampur, Chitwan for representing main season (May-August). The result of the experiment revealed that emergence count was highly significant for soil types and depth of sowing among the maize varieties tested. Manakamana-1 expressed lowest percentage (20 and 29%) followed by Arun-2 (22 and 33%) and Rampur Composite (24 and 39%) of emergence under red clay and sandy loam soils, respectively. Likewise, emergence count at 10 days after sowing was recorded highly significant for depth of showing indicating that irrespective of varieties higher the planting depth lower the emergence count.. There was a positive relation between depth of planting and length of mesocotyle resulting loss of seed vigor which furnished low emergence of the maize varieties. It is suggested that depth of sowing affect emergence percentage which consequently have effects on plant population maintenance. Hence, it was concluded that sowing below 7cm deep is not feasible for the tested varieties under red clay and sandy loam soils. DOI: http://dx.doi.org/10.3126/ajn.v1i0.7539 Agronomy Journal of Nepal (Agron JN) Vol. 1: 2010 pp.23-27

scholarly journals Regeneration of Bitter Gourd (Momordica charantia L.) from Leaf Segments and Root Tips

2013 ◽  
Vol 18 (2) ◽  
pp. 1-9
Author(s):  
MAZ Al Munsur ◽  
MS Haque ◽  
KM Nasiruddin ◽  
MJ Hasan
Keyword(s):  
Callus Induction ◽  
Genetic Improvement ◽  
Callus Formation ◽  
Plantlet Regeneration ◽  
Bitter Gourd ◽  
Regeneration Ability ◽  
Root Tips ◽  
Induction Frequency ◽  
Biochemical Studies ◽  
Leaf Segments

Experiments were carried out to study the callus induction and subsequent plantlet regeneration ability of leaf segments and root tips of bitter gourd. A combination of 1.0 mg L-1 2,4-D and 1.0 mg L-1 BAP produced the highest percentage of callus in leaf segments (78.75%) and 1.0 mg L-1 2,4-D combined with 0.5 mg L-1 BAP produced the highest callus frequency in root tips (72.5%). Using the combination of BAP at 2.0 mg L-1 and NAA at 0.3 mg L-1 improved the callus induction frequency (80.00%) in root tips. Root tips showed better response than leaf segments in callus induction. Leaf segments and root tips had equal percentage (65.0) of shoot regeneration upon culture of the calli on medium with 2.0 or 2.5 mg L-1 BAP with 0.2 mg L-1 IAA. The highest length (3.95 cm) of shoot from leaf segments was recorded with 2.5 mg L-1 BAP, 0.5 mg L-1 IBA and 0.2 mg L-1 GA3. The highest number of roots (6.75) and root length (2.45 cm) were found in 3.0 mg L-1 BAP with 0.1 mg L-1 NAA in root tips. The callus formation and regeneration ability of the explants have the potential for use in future biochemical studies and genetic improvement of bitter gourd.DOI: http://dx.doi.org/10.3329/pa.v18i2.17457 Progress. Agric. 18(2): 1 - 9, 2007

Sign in / Sign up

Export Citation Format

Share Document