In Vitro Characterization of Methotrexate-Loaded Poly(Lactic Acid) Microspheres of Different Molecular Weights

Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

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In Vitro Characterization of Lactic Acid Bacteria Isolated from Bovine Milk as Potential Probiotic Strains to Prevent Bovine Mastitis

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-017-9383-6 ◽

2018 ◽

Vol 11 (1) ◽

pp. 74-84 ◽

Cited By ~ 8

Author(s):

Matías S. Pellegrino ◽

Ignacio D. Frola ◽

Berardo Natanael ◽

Dino Gobelli ◽

María E.F. Nader-Macias ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Bovine Milk ◽

Bovine Mastitis ◽

In Vitro Characterization ◽

Probiotic Strains

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Preparation and Characterization of Poly(lactic acid) with Different Molecular Weights by Thermal Hydrolysis

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.440.19 ◽

2013 ◽

Vol 440 ◽

pp. 19-24 ◽

Cited By ~ 1

Author(s):

Jun Yan Yao ◽

Loogn Tak Lim ◽

Yu Jie Li

Keyword(s):

Molecular Weight ◽

Lactic Acid ◽

Hydroxyl Group ◽

Poly Lactic Acid ◽

Thermal Hydrolysis ◽

Molecular Weights ◽

Hydrolytic Reaction ◽

Structures And Properties ◽

Ft Ir

A feasible and effective method of thermal hydrolysis to prepare poly (lactic acid) (PLA) oligomers with different controlled molecular weight from PLA is presented in this paper. The thermal hydrolytic reaction was carried out by immerging PLA resin pellets in boiling distilled water for a certain period of time. Ester groups in PLA chains are hydrolytically degraded in the presence of water and thermal, so PLA oligomers with different molecular weight were prepared. The structures and properties of PLA oligomers were characterized by FT-IR, GPC, DSC, etc. The results showed that thermal hydrolytic reaction could effectively reduce the molecular weight of PLA, which declines with the increase of the thermal hydrolytic reaction time. Meanwhile, the content of terminal hydroxyl group, glass transition temperature, melting point of PLA oligomers prepared from thermal hydrolytic reactions exhibit gradual changes with the extension of the thermal hydrolytic time.

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