scholarly journals Use of umbilical cord blood culture in the diagnosis of early onset neonatal sepsis among high risk mothers

2021 ◽  
Vol 12 (12) ◽  
pp. 78-84
Author(s):  
Maitreyi Ojha ◽  
Ashish Pradhan ◽  
Sudip Dutta ◽  
Anamika Jaiswal
Keyword(s):  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Early Onset ◽  
Venous Blood ◽  
Peripheral Vein ◽  
Peripheral Venous Blood

Background: Early onset neonatal sepsis (EONS) is one of the important causes of morbidity and mortality in neonates. Its early diagnosis and prompt treatment is essential and any delay in the diagnosis can have serious consequences including neonatal death. Blood culture is the gold standard test for diagnosis of neonatal sepsis. Umbilical cord blood culture (UCBC) is a painless procedure and technically less challenging. We conducted this study to evaluate use of UCBC for the diagnosis of EONS and compared it with the results of peripheral venous blood culture (PVBC) reports. Aims and Objectives: The aim of the study was to evaluate UCBC for the diagnosis of EONS and compared it with the results of PVBC reports. Materials and Methods: This was a hospital-based prospective cohort study consisting of 100 neonates who were at risk of EONS. The study was conducted in the Department of Pediatrics Sikkim Manipal Institute of Medical Sciences Gangtok between January 2018 and December 2019. Neonates found to be at risk of development of EONS were included in this study on the basis of a predefined inclusion and exclusion criteria. Immediately after birth blood samples were collected from both umbilical cord and peripheral vein and were sent to bacteriology lab. Sensitivity, specificity, positive predictive value, and negative predictive value of both the samples were analyzed. Results: Out of 100 neonates in 32 (32%) EONS could be confirmed with positive sepsis screening results and/or demonstration of organisms on blood culture. Among the 32 neonates with EONS, 17 were found to be premature. The mean gestational age of newborns with EONS was found to be 35.2 weeks. The umbilical blood culture was found to have sensitivity and specificity of 100% and 74.4%, respectively, whereas peripheral vein blood culture was found to have sensitivity and specificity of 77.7% and 72.5%, respectively. The most common organism grown in our study was Escherichia coli. Conclusion: UCBC is painless and technically less challenging method of blood sampling. It has been found to have a higher sensitivity as well specificity for the diagnosis of EONS as compared to peripheral venous blood sample.

scholarly journals Umbilical Cord Blood Culture in General in the Diagnosis of Sageis Newborns

2020 ◽  
pp. 126-131
Author(s):  
Naredra P. Porval ◽  
Kanvikar Reshmi ◽  
D. B. Potdar ◽  
S. B. Karanjkar
Keyword(s):  
Risk Factors ◽  
Birth Weight ◽  
High Risk ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Venous Blood ◽  
Peripheral Venous Blood

Worldwide neonatal sepsis is among the most frequent causes of neonatal death. Various studies have tried to establish the relationship between prevalence of neonatal septicemia risk factors and bacteriological profiling, low birth weight, prematurity, etc. Current study was aimed to compare early onset of neonatal sepsis (EONS) among primigravida and multigravida mothers using umbilical cord blood (UCB) and peripheral venous blood (PVB) samples. It was also aimed to establish the utilization of umbilical cord blood culture (UCBC) in comparison to peripheral venous blood culture (PVBC) in identifying EONS. In present study the blood samples were collected from high risk neonates for the clinical blood culture and screening. Among the 75 neonates in the study, 24 (32.0%) were observed to have sepsis screen positive. Study of high risk neonates umbilical cord blood culture (UCBC) positivity was 17.3% while Peripheral Venous blood culture positivity was 5.3%. Moreover,  in this study all risk factors like Prematurity, Low birth weights, Premature rupture of membrane, and birth asphyxia were significantly (p<0.05) associated with UCBC growth/positivity. Low birth weight (86%) was mostly reported in the high risk neonates with other associated sepsis factors. Similarly maternal fever and prolonged rupture of membrane was highly significantly (p<0.01) associated with UCBC positivity. Gram negative bacterias  were  more  commonly found,  such as Pseudomonas (5.3%), followed by E. coli (4%), and Klebsiella (2.7%) and gram positive Streptococcus sp. (2.7%), etc. From our analysis it can be said that the UCBC has strong diagnostic outcomes as compared to the PVBC for etiological evaluation of bacterial sepsis in neonates at high risk.

scholarly journals Comparative assessment of umbilical cord blood with peripheral venous blood using hematological scoring system as an early predictive screening method for the detection of early-onset neonatal sepsis in the tertiary care center of Central India

2022 ◽  
Vol 13 (1) ◽  
pp. 118-122
Author(s):  
Sunil Arya ◽  
Gagandeep Shukla ◽  
Prachi Goyal ◽  
Urvashi Channa
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Scoring System ◽  
Venous Blood ◽  
Screening Method ◽  
Peripheral Venous Blood ◽  
Sepsis Screen

Background: Sepsis is one of the major causes of neonatal morbidity and mortality. Early recognition and diagnosis of early-onset neonatal sepsis (EONS) is difficult because of the variable and non-specific clinical presentation of this condition. Hence, there is a need for early predictive screening method for EONS. Aims and Objectives: To compare the umbilical cord blood Haematological Scoring System (HSS) with peripheral venous blood as an early predictive screening method for detection of EONS. Materials and Methods: 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in this prospective analytical study. Blood samples were collected from the umbilical cord and peripheral vein analyzed for hematological parameters, sepsis screen, and peripheral smear for HSS of Rodwell et al., send for blood culture. Blood cultures were performed as gold standard for diagnosing neonatal sepsis and sepsis screen was done to corroborate the diagnosis of neonatal sepsis. Results: Of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. HSS in umbilical cord blood (UCB) had Sensitivity-74.28%, Specificity-92.30%, PPV-83.87%, NPV-86.95% and HSS in PVB had Sensitivity-62.85%, Specificity- 87.69%, PPV-75.86%, NPV-81.69%. Conclusion: HSS score in UCB can be used as a simple, quick, cost-effective, and readily available screening test with decent sensitivity and high specificity, for the detection of EONS.

EVALUATION OF CORD BLOOD CULTURE AS AN EARLY PREDICTOR FOR THE DETECTION OF EARLY ONSET NEONATAL SEPSIS IN TERTIARY CENTRE OF CENTRAL INDIA.

2021 ◽  
pp. 84-86
Author(s):  
Gagandeep Shukla ◽  
Urvashi Channa
Keyword(s):  
Blood Culture ◽  
Cord Blood ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Sequential Sampling ◽  
Venous Blood ◽  
Central India ◽  
Neonatal Morbidity ◽  
Peripheral Venous Blood ◽  
Tertiary Centre

BACKGROUND : Neonatal sepsis is one of the major causes of neonatal morbidity and mortality. Peripheral venous blood culture and sensitivity is gold standard for the diagnosis of neonatal sepsis. Low sensitivity of blood culture in newborn is due to small volume of blood sample collected from neonates & antibiotics given before sampling. AIM : To evaluate the utility & compare the Umblical cord blood culture(UCBC) with Peripheral venous blood culture(PVBC) for detection of Early Onset Neonatal Sepsis(EONS) METHOD : 100 inborn neonates with two or more risk factors for EONS, chosen by sequential sampling method were included in this prospective analytical study. Blood samples were collected from umbilical cord and peripheral vein for culture. Sepsis screen was done to corroborate the diagnosis of neonatal sepsis. RESULT : Out of 100 neonates, 21 belongs to sepsis; 14 to probable sepsis; 65 to no sepsis. UCBC had Sensitivity-65.71% , Specicity-93.84%, PPV-85.18% , NPV-83.56% & PVBC had Sensitivity-60% , Specicity-95.38% , PPV-87.5% , NPV-81.57%. CONCLUSION : UCBC is simple and convenient method for the diagnosis of EONS compared to PVBC. Organisms grown are comparable to PVBC sample.

Umbilical Cord Blood Culture in Diagnosis of Early Onset Neonatal Sepsis

2020 ◽  
Vol 87 (10) ◽  
pp. 793-797 ◽  
Author(s):  
Ramraj Meena ◽  
Kailash Kumar Meena ◽  
Vivek Athwani ◽  
Sunil Gothwal ◽  
Ghan Shyam Bairwa ◽  
...  
Keyword(s):  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Early Onset

scholarly journals Umbilical Cord Blood for the Screening of Early Onset Neonatal Sepsis among Those at Risk of Infection

2021 ◽  
Vol 9 (3) ◽  
pp. 452-461
Author(s):  
  Dr. Mohammad Shakhawat Alam ◽  
Prof. M.A. Mannan ◽  
Dr. Sanjoy Kumer Dey ◽  
Dr. A Z M Raihanur Rahman ◽  
Prof. Mohammod Shahidullah
Keyword(s):  
At Risk ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Risk Of Infection

scholarly journals Study of umbilical cord blood culture in diagnosis of early-onset sepsis among newborns with high-risk factors

2013 ◽  
Vol 2 (4) ◽  
pp. 169 ◽  
Author(s):  
MitulBabubhai Kalathia ◽  
IlaMitulkumar Kalathia ◽  
PrakashAshokbhai Shingala ◽  
ParinNiranjanbhai Parmar ◽  
YogeshNarenedrabhai Parikh
Keyword(s):  
Risk Factors ◽  
High Risk ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Early Onset ◽  
High Risk Factors ◽  
Early Onset Sepsis

scholarly journals Eliminating Contamination in Umbilical Cord Blood Culture Sampling for Early-Onset Neonatal Sepsis

2021 ◽  
Vol 9 ◽  
Author(s):  
Vilmaris Quinones Cardona ◽  
Vanessa Lowery ◽  
David Cooperberg ◽  
Endla K. Anday ◽  
Alison J. Carey
Keyword(s):  
Quality Improvement ◽  
Blood Culture ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Peripheral Blood ◽  
Early Onset ◽  
Clinical Sepsis ◽  
Early Onset Sepsis ◽  
Antibiotic Duration

Introduction: Despite the advantages of umbilical cord blood culture (UCBC) use for diagnosis of early onset sepsis (EOS), contamination rates have deterred neonatologists from its widespread use. We aimed to implement UCBC collection in a level III neonatal intensive care unit (NICU) and apply quality improvement (QI) methods to reduce contamination in the diagnosis of early onset sepsis.Methods: Single-center implementation study utilizing quality improvement methodology to achieve 0% contamination rate in UCBC samples using the Plan-Do-Study-Act (PDSA) model for improvement. UCBC was obtained in conjunction with peripheral blood cultures (PBC) in neonates admitted to the NICU due to maternal chorioamnionitis. Maternal and neonatal characteristics between clinical sepsis and asymptomatic groups were compared. Process, outcome, and balancing measures were monitored.Results: Eighty-two UCBC samples were collected in addition to peripheral blood culture from neonates admitted due to maternal chorioamnionitis. Ten (12%) neonates had a diagnosis of clinical sepsis. All PBCs were negative and 5 UCBCs were positive in the study period. After 2 PDSA cycles, there was special cause variation with improvement in the percent of contaminated samples from 7.3 to 0%. There was no change in antibiotic duration among asymptomatic neonates.Conclusions: Implementation of UCBC for the diagnosis of EOS in term infants is feasible and contamination can be minimized with the implementation of a core team of trained providers and a proper sterile technique without increasing antibiotic duration.

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