scholarly journals Strong mosquitocidal Bacillus thuringiensis from Mt. Everest

Our Nature ◽  
1970 ◽  
Vol 5 (1) ◽  
pp. 67-69
Author(s):  
Upendra Thapa Shrestha ◽  
Gyan Sunder Shahukhal ◽  
Kiran Babu Tiwari ◽  
Subarna Pokhrel ◽  
Anjana Singh ◽  
...  

Bacillus thuringiensis strains were isolated from soil samples collected from Khumbu Base Camp of the Everest region and characterized by standard microbiological techniques viz. colonial and morphological characteristics, and biochemical tests. Insect bioassay of each isolate was performed by standard method using mosquito larva. Among ten randomly selected isolates, one isolate showed the highest insecticidal activity against Dipteron insects. Keywords: Insect-bioassay, Isolates, Khumbu region, Mosquitocidal, Mosquito larvadoi:10.3126/on.v5i1.800Our Nature (2007)5:67-69

2006 ◽  
Vol 72 (5) ◽  
pp. 924-930 ◽  
Author(s):  
Guangjun Wang ◽  
Jie Zhang ◽  
Fuping Song ◽  
Jun Wu ◽  
Shuliang Feng ◽  
...  

2007 ◽  
Vol 10 (2) ◽  
pp. 137-143
Author(s):  
Joong Nam Kang ◽  
Jong Yul Roh ◽  
Sang Chul Shin ◽  
Sang-Hyun Koh ◽  
Yeong Jin Chung ◽  
...  

2011 ◽  
Vol 77 (11) ◽  
pp. 3663-3668 ◽  
Author(s):  
Valeria Guidi ◽  
Nicola Patocchi ◽  
Peter Lüthy ◽  
Mauro Tonolla

ABSTRACTRecurrent treatments withBacillus thuringiensissubsp.israelensisare required to control the floodwater mosquitoAedes vexansthat breeds in large numbers in the wetlands of the Bolle di Magadino Reserve in Canton Ticino, Switzerland. Interventions have been carried out since 1988. In the present study, the spatial distribution of restingB. thuringiensissubsp.israelensisspores in the soil was measured. TheB. thuringiensissubsp.israelensisconcentration was determined in soil samples collected along six transects covering different elevations within the periodically flooded zones. A total of 258 samples were processed and analyzed by quantitative PCR that targeted an identical fragment of 159 bp for theB. thuringiensissubsp.israelensis cry4Aaandcry4Bagenes.B. thuringiensissubsp.israelensisspores were found to persist in soils of the wetland reserve at concentrations of up to 6.8 log per gram of soil. Continuous accumulation due to regular treatments could be excluded, as the decrease in spores amounted to 95.8% (95% confidence interval, 93.9 to 97.7%). The distribution of spores was correlated to the number ofB. thuringiensissubsp.israelensistreatments, the elevation of the sampling point, and the duration of the flooding periods. The number ofB. thuringiensissubsp.israelensistreatments was the major factor influencing the distribution of spores in the different topographic zones (P< 0.0001). These findings indicated thatB. thuringiensissubsp.israelensisspores are rather immobile after their introduction into the environment.


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