scholarly journals Study of the influence of interfering ions of heavy metals and various sample preparation on the determination of mercury impurity in protamine sulfate by stripping voltammetry

2021 ◽  
pp. 12-19
Author(s):  
N.O. Kim ◽  
◽  
E.A. Ivanovskaya ◽  
Keyword(s):  
Sample Preparation ◽  
High Selectivity ◽  
Sodium Tungstate ◽  
Stripping Voltammetry ◽  
Mercury Content ◽  
Protamine Sulfate ◽  
Bidistilled Water ◽  
Special Sample Preparation ◽  
Interfering Ions

Aim. To determine the content of mercury in protamine sulfate samples with different sample preparation. To study the effect of interfering ions on the content of mercury impurity in protamine sulfate by stripping voltammetry (SV). Materials and methods. We used a solution for injection of protamine sulfate, batches 515091, 514062, 514111; for sample preparation — various options for dilution and precipitation. Dilution was carried out with bidistilled water, and the precipitation of proteins — with sodium tungstate in 2 options. Mercury impurities were determined by the SV method. Results. The mercury content in protamine sulfate samples was 0.000417 ± 0.00140 and 0.000420 ± 0.00152 mg/l when diluted with water 1 : 2 and 1 : 1 respectively and 0.000462 ± 0.00131 and 0.000459 ± 0.00121 when precipitated with tungstate in options 1 and 2 respectively. With the addition of an interfering ion, for example, Cu2+, the content of mercury in the medicinal product was 0.000606 ± 0.00015, 0.000452 ± 0.00013 and 0.0004212 ± 0.00011 mg/l for protamine sulfate batches 514062, 515091 and 514111 respectively, which does not exceed the values determined by the product specification. The addition of Pb2+, Fe2+, Zn2+, Cu2+, and Cd2+ ions also had no effect on the determination of the mercury content in protamine sulfate samples. Conclusion. To determine the mercury impurity in the protamine sulfate, special sample preparation is not required. Ions of Cu2+, Pb2+, Fe2+, Cd2+, Zn2+ do not affect the result of determining the mercury content in protamine sulfate samples by the SV method, which indicates a high selectivity of the method used.

scholarly journals Square Wave Anodic Stripping Voltammetry Determination of Arsenic (III) onto Carbon Electrodes by Means of Co-deposition with Silver

2018 ◽  
Vol 62 (2) ◽  
Author(s):  
Sandra Perla Méndez Cortés ◽  
Carlos Andrés Galán Vidal ◽  
José Antonio Rodríguez Ávila ◽  
Giaan Arturo Álvarez Romero ◽  
María Elena Páez Hernández
Keyword(s):  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Carbon Electrodes ◽  
Electrochemical Assay ◽  
Analytical Strategy ◽  
Glassy Carbon Electrodes ◽  
Square Wave ◽  
Anodic Stripping ◽  
Interfering Ions

In the present study an electrochemical assay for the detection of arsenic (III) by means of square wave anodic stripping voltammetry with co-deposition of silver at screen printed and glassy carbon electrodes is presented. This methodology shows a good behavior in presence of dissolved oxygen with appropriate detection limits to monitoring the water quality according to the guidelines values. The evaluation of most important interfering ions (Cl<sup>-</sup>, Hg<sup>2+</sup>, Pb2<sup>+ </sup>and Cu<sup>2+</sup>) is also described. The proposed analytical strategy demonstrated to be suitable for decentralized arsenic analysis and can be easily integrated to portable instrumentation commercially available.

scholarly journals Determination of Mercury Impurities in Protamine Sulfate Medicament by Stripping Voltammetry

2019 ◽  
Vol 8 (2) ◽  
pp. 103-107
Author(s):  
N. O. Kim ◽  
E. A. Ivanovskaja
Keyword(s):  
Stripping Voltammetry ◽  
Sulfate Solution ◽  
Mercury Content ◽  
Protamine Sulfate ◽  
Polymer Composition ◽  
Mercury Determination ◽  
Graphite Electrodes ◽  
Accumulation Time ◽  
Pharmaceutical Substance ◽  
Mercury Analysis

Introduction. The article presents data of the quantitative mercury determination in an aqueous solution of the pharmaceutical substance protamine sulfate by stripping voltammetry on graphite electrodes modified with a gold-polymer composition. The existing method to determine the mercury impurity in protamine sulfate according to the European Pharmacopoeia – multistage titration with dithizone – is laborious and does not allow to achieve repeatability.Aim. Analyzing the content of bioaccumulative mercury impurities in a protamine sulfate solution by stripping voltammetry. Materials and methods. Protamine Sulfate Substance (Manufacturer Alps Pharmaceutical Industry Co.Ltd). Experimental data were obtained on a TA-4 semi-automatic analyzer (OOO NPP Tomianalit, Tomsk) with VALabTx software.Results and discussion. We adapted the method for mercury determination in water and presented the results of its testing on protamine sulfate solution. The results of testing the electrode operation using the «entered-found» method are given, the results of a single mercury analysis were included in the interval 0, 0038–0,0063 mg/l, which confirms their readiness for work. This interval is registered in the methodology and means the limits of permissible deviations. The limits of detection of mercury on the device are 10-6 –10-10 mol/l. We prepared a solution with concentrations of 10 mg/m from the protamine sulfate substance and measured it on prepared electrodes. The mercury content in the test sample was 0.00034 mg/l. According to the certificate of protamine sulfate, the mercury content should be ≤10 ppm. We studied the effect in changing of accumulation time. The optimal accumulation time was 80 seconds, because under standard conditions, not all mercury concentrates on the electrode surface, and above 80 seconds there is no significant increase in mercury content.Conclusion. The proposed method for mercury determination in water by stripping voltammetry on graphite electrodes modified with a gold-polymer composition can be adapted to determine bioaccumulative mercury in the pharmaceutical substance protamine sulfate. The findings suggest that this method can be used in quality control of medicines, as an alternative express method for mercury impurities determination to the existing multistage titration method with dithizone.

scholarly journals Cold Vapor Atomic Absorption and Microwave Digestion for the Determination of Mercury in Honey, Pollen, Propolis and Bees of Greek Origin

2016 ◽  
Vol 18 (4) ◽  
pp. 690-696
Keyword(s):  
Sample Preparation ◽  
Atomic Absorption ◽  
Honey Bees ◽  
Microwave Digestion ◽  
Mercury Content ◽  
Mercury Contamination ◽  
Cold Vapor ◽  
Cold Vapor Atomic Absorption ◽  
Special Concern

<div> <p>In the present study a fast and reliable cold vapor atomic absorbance method was developed and validated for the determination of mercury in four beehive matrices (honey, pollen, propolis and bees) from Greece. For the sample preparation microwave digestion with H<sub>2</sub>O<sub>2</sub> and HNO<sub>3 </sub>in closed vessels under pressure was applied. The analysis of the apiary products showed that the mercury content is &lt; 0.05 μg g<sup>-1</sup> for honey, &lt; 0.06 μg g<sup>-1</sup> for pollen, &lt; 0.3 μg g<sup>-1</sup> for propolis and &lt; 0.8 μg g<sup>-1</sup> for honey bees d.w. The findings of the present study, in comparison with the literature data, do not trigger a special concern regarding mercury contamination of honey, pollen, propolis and bees from any of the sampled areas in Greece.&nbsp;</p> </div> <p>&nbsp;</p>

Determination of Hg2+ Based on the Selective Enhancement of Peroxidase Mimetic Activity of Hollow Porous Gold Nanoparticles

NANO ◽  
2017 ◽  
Vol 12 (04) ◽  
pp. 1750050 ◽  
Author(s):  
Mingzhen Guo ◽  
Jiang He ◽  
Shuang Ma ◽  
Xiaohan Sun ◽  
Mingda Zheng
Keyword(s):  
Gold Nanoparticles ◽  
Total Mercury ◽  
High Selectivity ◽  
Yellow River ◽  
Tap Water ◽  
Mercury Content ◽  
High Catalytic Activity ◽  
Porous Gold ◽  
Selective Enhancement

In this paper, a novel colorimetric biosensor for Hg[Formula: see text] is presented based on Hg[Formula: see text] stimulated peroxidase-like activity of hollow porous gold nanoparticles (HPGNPs). Under mild conditions, HPGNPs show high catalytic activity toward the oxidation of 3, 3[Formula: see text], 5, 5[Formula: see text]-tetramethylbenzidine (TMB) using H2O2 as an oxidant. The peroxidase-like activity of HPGNPs is “switched-on” selectively in the presence of Hg[Formula: see text], which permitted a novel and facile colorimetric sensor for Hg[Formula: see text]. This method exhibits many merits like high selectivity and sensitivity. As low as 18.5[Formula: see text]nM Hg[Formula: see text] could be determined at a linear range from [Formula: see text][Formula: see text]M to [Formula: see text][Formula: see text]M. This method is successfully applied for the determination of total mercury content in tap water and Yellow River which indicates that the method has a great potential for the routine determination of Hg[Formula: see text] in environmental samples.

scholarly journals Toxico-ecological Analysis of Mercury-contained Waste Management in Ukraine

2020 ◽  
Vol 89 (2) ◽  
pp. 48-57
Author(s):  
E.G. Pykhteyeva ◽  
D.V. Bol'shoy ◽  
L.M. Shafran
Keyword(s):  
Sample Preparation ◽  
State Level ◽  
Mercury Content ◽  
Ecological Analysis ◽  
Industrial Facilities ◽  
Working Zone ◽  
Relevant Today ◽  
Key Words Mercury ◽  
Proper Disposal

Relevance. The multifaceted intersectoral problem of human security in contact with mercury has gained global significance in the twentieth century, it is relevant today. Considerable attention is paid to the environmental and hygienic aspects of mercury hazards in the field related to the production and disposal of waste containing Hg. The Aim of the Research. Analyze the data of own research on mercury content in the environment in connection with the management of mercury-containing waste and justify a set of preventive environmental and hygienic, technological and organizational measures to improve safety, public health and improve the environment in the region and the country in general. Materials and Methods. Sampling, sample preparation and determination of mercury content in environmental facilities and bioassays were performed according to MI “Methodical instructions for the determination of mercury in biological material, air, drinking water, food products and the environment.” The concentration of mercury was measured by the method of AAS “cold steam” on the upgraded device “Yulia-2M”. The mercury content in soil and air at the spontaneous dump of mercury lamps in Odessa region, on the border of the sanitary protection zone and in the air of the working zone of the enterprise for utilization of mercury lamps was studied. For sample preparation, chemically pure nitric acid (56%) was used, to reduce mercury during the analysis of chemically pure tin (II) chloride. Results and Conclusions. Mercury lamps have been shown to be hazardous to the environment and human health without proper disposal. Mercury lamps should be collected and disposed of as hazardous waste not only in industrial facilities but also in the general public. The formation of spontaneous unauthorized landfills of mercury lamps is unacceptable. The problem of environmental monitoring needs to be addressed at the state level with the mandatory involvement of specialists in the field of hygiene, toxicology and analytical chemistry of heavy metals in cases of violations to assess the level of danger and make informed decisions on approaches to elimination of mercury sources. Key Words: mercury, mercury lamps, ecology.

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