scholarly journals Correction of imbalance of essentialaminoacids of blood plasma in Patients with stableangina

2021 ◽  
pp. 36-41
Author(s):  
T. V. Bohdan ◽  
D. A. Pliskevich ◽  
V. V. Bohdan ◽  
Y. O. Моshkovska ◽  
O. V. Savchenko
Keyword(s):  
Amino Acid ◽  
Blood Plasma ◽  
Stable Angina ◽  
Therapy Group ◽  
Essential Amino Acids ◽  
Automatic Amino Acid Analyzer ◽  
Basic Therapy ◽  
Amino Acid Analyzer ◽  
Spectrum Of Patients ◽  
Amino Acid Spectrum

Introduction. Ischemic heart disease is the leading nosology calunit in the structure of cardiovascular diseases interms of disability and mortality among the population of Ukraine. The purpose. To improve the treatment of patients with stable angina by studying the effect of L-arginine on the balance of essential amino acids in blood plasma. Material and methods. It was examined 85 patients with stable angina. They were divided into two groups: group Ipatients received antianginal basic therapy, group II patients received basic antianginal therapy and L-arginine. The amino acid spectrum of patients' blood plasma was studied by ion-exchange liquid column chromatography, using an automatic amino acid analyzer T-339 Microtechna (Czech Republic, Prague). Results and discussion. In patients with stable angina who received basic therapy and L-arginine, in contrast to patients who received only basic therapy, plasma levels of arginine became normalized, which probably contributes to the synthesis of NO. The level of valine, leucine and isoleucine, which provide the synthesis of acyl-CoA and succinyl-CoA, became also normalized.Conclusion. Administration of L-arginine to patients with stable angina together with antianginal therapy helps to correct plasma amino acid imbalances, which is likely to effectively affect the course of the disease and prognosis.

scholarly journals The effect of L-arginine on the balance of essential amino acids in plasma of the patients with stable angina

2020 ◽  
pp. 25-30
Author(s):  
T. V. Bogdan ◽  
V. O. Onishchenko ◽  
V. V. Bogdan ◽  
O. V. Savchenko
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Stable Angina ◽  
Clinical Medicine ◽  
Therapy Group ◽  
Essential Amino Acids ◽  
Automatic Amino Acid Analyzer ◽  
Basic Therapy ◽  
Spectrum Of Patients

Background. Despite the significant achievements of clinical medicine in the prevention, diagnosis and treatment of coronary heart disease, the levels of morbidity, disability and mortality among the population of Ukraine from this pathology remain consistently high. The purpose. To improve the treatment of patients with stable angina by studying the effect of L-arginine on the balance of essential amino acids in blood plasma. Material and methods. It was examined 67 patients with stable angina. They were divided into two groups: group Ipatients received antianginal basic therapy, group II patients received basic antianginal therapy and L-arginine. The amino acid spectrum of patients' blood plasma was studied by ion-exchange liquid column chromatography, using an automatic amino acid analyzer T-339 Microtechna (Czech Republic, Prague). Results and discussion. In patients with stable angina who received basic therapy and L-arginine, in contrast to patients who received only basic therapy, plasma levels of arginine became normalized, which probably contributes to the synthesis of NO. The level of valine, leucine and isoleucine, which provide the synthesis of acyl-CoA and succinyl-CoA, became also normalized. Conclusion. Administration of L-arginine to patients with stable angina together with antianginal therapy helps to correct plasma amino acid imbalances, which is likely to effectively affect the course of the disease and prognosis.

scholarly journals Influence of bisoprolol and nebivolol on the spectrum of substituted amino acids in blood plasma of patients with stable angina

2021 ◽  
pp. 104-108
Author(s):  
T.V. Bogdan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Stable Angina ◽  
Essential Amino Acids ◽  
Taurine Level ◽  
Amino Acid Imbalance ◽  
Sulfur Containing ◽  
Cystine Level ◽  
Amino Acid Spectrum ◽  
Methionine Level

For many years, ß-blockers have been used to treat patients with coronary heart disease. In patients with stable angina, the effect of ß-blockers on the amino acid spectrum of blood serum has been studied. Its violation is considered as one of the pathogenetic links in the development of atherosclerosis. The study showed a more efficient action of nebivolol compared to bisoprolol on the amino acid imbalance. In patients with stable angina against the background of antianginal therapy, which included bisoprolol and nebivolol, there was a significant decrease in the total amount of amino acids, the amount of essential amino acids, and the amount of essential amino acids compared to the treatment. Against the background of the nebivolol treatment, there is a positive dynamics of sulfur-containing amino acids (methionine level was maintained, taurine level was significantly increased, cystine level was normalized), isoleucine level was normalized, which indirectly indicates cardioprotective and angiprotective effect of nebivolol.

scholarly journals Evaluation of Amino Acid and Vitamin B2 and E Composition of Genetically Modified Antidwarf Mosaic Maize by Automatic Amino acid Analyzer and HPLC

2013 ◽  
Vol 25 (6) ◽  
pp. 3525-3526 ◽  
Author(s):  
Pingmei Yan ◽  
Qing Wang ◽  
Xiaoyan Yan ◽  
Xiao-Hui Chang ◽  
Jian-Zhong Du ◽  
...  
Keyword(s):  
Amino Acid ◽  
Genetically Modified ◽  
Vitamin B2 ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

Quantitative decarboxylation of [ 14 C]amino acids using automatic amino acid analyzer

1976 ◽  
Vol 72 (1-2) ◽  
pp. 305-309
Author(s):  
David E. Peterson ◽  
Nyles W. Charon ◽  
Russell C. Johnson
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Automatic Amino Acid Analyzer ◽  
Amino Acid Analyzer

Ionenaustausch-chromatographische Untersuchungen über die freien Aminosäuren und deren Derivate in Drosophila melanogaster

1965 ◽  
Vol 20 (4) ◽  
pp. 307-312 ◽  
Author(s):  
P. S. Chen ◽  
F. Hanimann
Keyword(s):  
Amino Acids ◽  
Drosophila Melanogaster ◽  
Amino Acid ◽  
Partition Chromatography ◽  
Automatic Amino Acid Analyzer ◽  
Amino Butyric Acid ◽  
Amino Acid Analyzer ◽  
Tyrosine Phosphate ◽  
Distinct Separation ◽  
Metabolic Significance

By using the automatic amino acid analyzer (model 120 B, Beckman) the free ninhydrin-positive components in the methanol extracts of Drosophila melanogaster were fractionated. Compared to the conventional paper partition chromatography the great advantage of this technique is the distinct separation of such amino acids like leucine, isoleucine, phenylalanine, valine, methionine, γ-amino-butyric acid as well as the basic components ornithine, lysine, histidine and arginine. Furthermore, the occurrence of phosphoserine, tyrosine phosphate, ethanolamine, phosphoethanolamine and glycerophosphoethanolamine was identified. The metabolic significance of these substances is discussed.

Comparative Analysis of Some Essential Amino Acids and Available Lysine in Acacia colei and A. tumida Seeds Using Chemical Methods and an Amino Acid Analyzer

2013 ◽  
Vol 96 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Olumuyiwa S Falade ◽  
Steve R A Adewusi
Keyword(s):  
Amino Acids ◽  
Comparative Analysis ◽  
Amino Acid ◽  
Essential Amino Acids ◽  
Acid Base ◽  
Chemical Methods ◽  
Amino Acid Analyzer ◽  
Available Lysine

Abstract Methionine, cysteine, tryptophan, and available lysine were determined in Acacia colei and A. tumida seeds and some cereals using chemical methods, and the results were compared to those obtained using an amino acid analyzer. Ba(OH)2 hydrolysis gave the best result of the three methods of hydrolysis (acid, base, and enzyme) tried. Oxidized methionine, cysteine, and tryptophan were not detected, but S-carboxyethylcysteine was estimated as cysteine by the chemical methods, thus overestimating cysteine's content in Acacia seeds. Tryptophan and methionine were higher in cereals than in Acacia seeds, while the level of cysteine and available lysine was higher in Acacia seeds than in cereals. These results agreed with values obtained using the amino acid analyzer and could therefore be used in low budget laboratories.

Effects of atropine on plasma amino acid profiles and on the synthesis of individual milk proteins in dairy cows fed with pasture

2003 ◽  
Vol 70 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Martin J Auldist ◽  
Catherine M Menzies ◽  
Colin G Prosser
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Latin Square ◽  
Milk Proteins ◽  
Protein Composition ◽  
Essential Amino Acids ◽  
Plasma Amino Acid ◽  
Milk Components ◽  
In Blood Plasma

Effects of atropine on blood plasma amino acid profile and on the yields and concentration of milk components were investigated in 12 Friesian cows in early lactation. Cows were housed indoors and fed with cut pasture ad libitum. Each cow received four treatments over 12 d during a replicated 4×4 Latin square experiment. Treatments were: control (saline); low dose (L; 30 μg atropine/kg body weight (BW)); medium dose (M; 40 μg atropine/kg BW); and 2×L dose, 2 h apart (2×L). On each of four treatment days, cows were milked at about 7.00, after which treatments were administered by subcutaneous injection. Cows were milked again at 2 h, 6 h and 10 h after injection. Milk samples were collected at each milking. Immediately after the 2 h milking, blood samples were drawn from each cow and the second injection was given for the 2×L treatment. Atropine reduced hourly milk yield, and concentrations and hourly yields of total protein, casein, whey protein, α-casein, β-casein, κ-casein, β-lactoglobulin and α-lactalbumin, but by differing amounts. Milk concentrations of bovine serum albumin and immunoglobulin G were increased by atropine, and overall yields of these proteins were mostly unchanged. Atropine lowered concentrations of most, but not all, amino acids in blood plasma, with essential amino acids reduced more than non-essential amino acids. Concentrations of α-amino N in whole blood, and glucose and insulin in blood plasma, fell after atropine injection. There was no difference between the L and M doses of atropine, but the 2×L dose had greater effects on milk composition than the single doses. For yields of milk and milk components, the effect of the 2×L dose was also more persistent. The results highlight the differential synthesis of individual milk proteins, and suggest that atropine might be useful for evaluating the mechanisms regulating milk protein composition.

scholarly journals Degradation and outflow of amino acids from the rumen of sheep

1989 ◽  
Vol 61 (2) ◽  
pp. 397-408 ◽  
Author(s):  
D. J. Cottle ◽  
W. Velle
Keyword(s):  
Amino Acids ◽  
Polyethylene Glycol ◽  
Amino Acid ◽  
Blood Plasma ◽  
Relative Rate ◽  
Essential Amino Acids ◽  
Feed Supplement ◽  
Dose Dependent ◽  
In Blood Plasma

1. In hay-fed, cannulated sheep the apparent degradation in and outflow from the rumen were determined for graded doses of mixtures of the amino acids lysine, threonine and methionine, administered intraruminally and using polyethylene glycol (PEG) as a liquid marker. The doses ranged between 2.5 and 15 g for each amino acid in the mixtures.2. Relative rate of apparent degradation in the first 4 h was highest for lysine, and lowest for methionine. The apparent degradation in 24 h was highest for lysine and lowest for threonine. Conversely the fraction flowing out of the rumen in intact form in 24 h was highest for threonine and lowest for lysine. Rates of apparent degradation as well as outflow were dose-dependent.3. The validity of the estimated outflow of amino acids from the rumen was corroborated by measurements of concentrations of the amino acids in duodenal contents and in blood plasma which were also dose-dependent.4. It was concluded that part of the requirement for the essential amino acids threonine and methionine may be met, even when these amino acids are delivered in unprotected form, given as a feed supplement.

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