Demonstrative protest crime: the limits of criminological analysis

Classical and network protest always assumes mass character. In this regard, the study of protest movements should assume: a) The analysis of many protest actions; b) the analysis of each individual protest action as a manifestation of the phenomenon of collective behavior. At the same time, the first level is important in the context of ensuring the representativeness of the second type of research. «Collective action» and «action of a team member» are fundamentally important. The regulatory system always deals with the assessment of the behavior of a particular person. In this regard, criminology and criminal law should not focus on the protest actions themselves, but on the behavior of individuals within the framework of these actions. In the theoretical analysis of the behavior of these individual participants of mass actions, it is necessary to distinguish: a) actions performed during a mass action, and actions that ensure the protest action itself, which can be performed both before and after it; b) actions of organizers, inspirers, leaders of protest actions, and actions of ordinary participants of the protest action; c) actions related to the organization and participation in mass actions coordinated by the official authorities, and in those not coordinated by the official authorities; d) lawful actions and illegal actions, and among the latter - criminally illegal and administratively illegal; e) intellectual, informational actions, and physical actions. To describe these actions, the article suggests using the term «demonstrative protest crimes». They are: a) associated with a well-defined sphere of public relations that arise in the process of interaction between a person, society and the authorities; b) they are imbued with the unity of motivational factors and the characteristics of the personality of the participants; c) they have a common determinative complex and a common mechanism for their commission; d) they have a distinct separation by the place and time of their commission; e) they assume a specific type and mechanism for the implementation of preventive measures. These signs allow us to consider demonstrative protest crimes as a separate, independent type of crime.

Integrative Proteomic Characterization of Trace FFPE Samples in Early-Stage Gastrointestinal Cancer

Background The surveillance and therapy of early-stage cancer would be better for patients’ prognosis. However, the extreme trace amount of tissue samples in different stages have limited in portraying the characterization of early-stage cancer. Therefore, we focused on and presented comprehensive proteomic and phosphoproproteomic profiling of the trace FFPE samples from early-stage gastrointestinal cancer, and then explored the potential biomarkers of early-stage gastrointestinal cancer. Methods In this study, a quantitative proteomic method with chromatography with mass spectrometry (LC-MS/MS) was used to analyse the proteomic difference between the trace early-stage esophageal squamous cell carcinoma (EESCC) and early-stage duodenum adenocarcinoma cancer (EDAC). Results We identified ~6,000 proteins and >10,000 phosphosites in single trace FFPE samples. The distinct separation of EESCC and EDAC illustrated the functions of cell cycle (RB1 T373, EGFR T693) in EESCC, and the positive impacts of apoptosis, metabolic processes (MTOR and MTOR S1261) in EDAC. Furthermore, we deconvoluted the immune infiltration of early-stage gastrointestinal cancer, in which higher immune cell signatures were detected in EDAC, and showed the specific cytokines in EESCC and EDAC. We performed kinases-substates relationship analysis and elucidated the specific proteomic kinase characterization of EESCC and EDAC, and proposed the medicative effects and corresponding drugs for EESCC and EDAC at the clinic.Conclusion We disclosed the specific immune characterization of the early-stage gastrointestinal cancer, and presented potential makers of EESCC (EGFR, PDGFRB, CDK4, WEE1) and EDAC (MTOR, MAP2K1, MAPK3). This study represents a major stepping stone towards investigating the carcinogenesis mechanism of gastrointestinal cancer, and providing a rich resource for medicative strategy in the clinic.

Out of place: Rewriting a landscape signature

<p>South Wairarapa, in New Zealand’s lower North Island presents an interesting collision; the landscapes forms and features evidence distinct separation between traditional landscape values and recent European economic developments – a separation evident in most New Zealand regions. Combined with its low urban socio-economic outlook and the utilisation of unsustainable farming practices, the region is in decline both ecologically and socially. Near irreversible damage has been done to the landscape. Engineered floodplain manipulation, land clearing, and intensive individualistic farming has resulted in continuing land degradation, flooding, droughts, severe waterway contamination, pollution, and habitat destruction. Utilising a design led approach in a three-scale investigation with focus assumed on the Ruamahanga River catchment, this thesis investigation explores how designed intervention with regards to the Wairarapa waterway systems can promote a re-emergence of place and placement in Wairarapa to reflect an integrated relationship with the processes of one’s landscape. It discusses how this interaction could be landscape transformative, re-introduce the prosperities of historic Wairarapa, promote sustainable farming practices, and recover declining ecologies. The investigation also discusses the wider New Zealand issue of rural waterway acknowledgement, and its relationship to place, culture, and identity discourse. This study has been ethically approved. Approval number 22990.</p>

Out of place: Rewriting a landscape signature

<p>South Wairarapa, in New Zealand’s lower North Island presents an interesting collision; the landscapes forms and features evidence distinct separation between traditional landscape values and recent European economic developments – a separation evident in most New Zealand regions. Combined with its low urban socio-economic outlook and the utilisation of unsustainable farming practices, the region is in decline both ecologically and socially. Near irreversible damage has been done to the landscape. Engineered floodplain manipulation, land clearing, and intensive individualistic farming has resulted in continuing land degradation, flooding, droughts, severe waterway contamination, pollution, and habitat destruction. Utilising a design led approach in a three-scale investigation with focus assumed on the Ruamahanga River catchment, this thesis investigation explores how designed intervention with regards to the Wairarapa waterway systems can promote a re-emergence of place and placement in Wairarapa to reflect an integrated relationship with the processes of one’s landscape. It discusses how this interaction could be landscape transformative, re-introduce the prosperities of historic Wairarapa, promote sustainable farming practices, and recover declining ecologies. The investigation also discusses the wider New Zealand issue of rural waterway acknowledgement, and its relationship to place, culture, and identity discourse. This study has been ethically approved. Approval number 22990.</p>

Phenolic composition of Croatian olive leaves and their infusions obtained by hot and cold preparation

Leaves and infusions of six Croatian olive cultivars grown in an organic orchard under the same agronomic conditions were characterised by high-performance liquid chromatography-ultraviolet/visible spectrophotometry (HPLC-UV/VIS). The total identified phenols in leaves ranged from 3 818 mg 100 g^–1 [cultivar Istarska crnica (IC)] to 10 572 mg 100 g^–1 of dry mass [cultivar Oblica (OB)]. The canonical discriminant analysis (CDA) provided a distinct separation of cultivars based on leaves' phenolic profiles. Hot- and cold-water infusions (200 mL) were prepared from 1 g of dry leaves. The average transfer rate of the total phenols in the cold-water infusions was 40% (25 °C/30 min), while in the hot-water infusions was 63% (75 °C/3 min) and 76% (100 °C/3 min). Although the cold-water infusions had the lowest transfer rate, they contained important levels of hydroxytyrosol derivatives ranging from 16.6 mg 200 mL^–1 to 36.5 mg 200 mL^–1 depending on the cultivar. Therefore, both hot and cold preparations are effective in obtaining antioxidant-rich natural beverages.

Small in size, big on taste: Metabolomics analysis of flavor compounds from Philippine garlic

Philippine garlic (Allium sativum L.) is arguably known to pack flavor and aroma in smaller bulbs compared to imported varieties saturating the local market. In this study, ethanolic extracts of Philippine garlic cultivars were profiled using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). γ-Glu dipeptides, oligosaccharides and lipids were determined in Philippine garlic cultivars through bioinformatics analysis in GNPS Molecular Networking Platform and fragmentation analysis. Multivariate statistical analysis using XCMS Online showed the abundance of γ-Glu allyl cysteine in Batanes-sourced garlic while γ-Glu propenyl cysteine, γ-Glu methyl cysteine, and alliin are enriched in the Ilocos cultivar. Principal component analysis showed that the γ-Glu dipeptides found in local garlic influenced their distinct separation across PC1 from imported varieties. This presence of high levels of γ-Glu dipeptides and probiotic oligosaccharides may potentially contribute to the superior flavor and nutritional benefits of Philippine garlic.

Karakteristik Arsitektural Bangunan Indis Pada Perumahan Pegawai PJKA Pengok Blok A & B di Yogyakarta

Indis house is a designation for the living house that occupied by both Dutch and the Indo-Dutch that built in the Dutch Colonial age in style of combining between Europe architectural forms especially Dutch and local architectural (Java). This combining created architectural characteristics of Indis house that are different from traditional house. In planning and designing process, Indis house is influenced by many factors such as the condition of surroundings, tropical climate which are very different from the climate in Netherland, social status, and also combining forms between Europe architecture and local architecture. The housing of Pegawai Perusahaan Jawatan Kereta Api Pengok at block A and B in Yogyakarta as one of the Indis housing of Dutch Colonial inheritance has uniqueness that reflected on its setting whith background of many factors influenced it. This literatur uses rationalistic-qualitative metheod in approach to theoretical framework that is built base on assessment parameters of architectural characterictics of living house according to Habraken (1978) including spatial system, physical system, and stylistic system. The Housing of PJKA Pengok at Block A and B in Yogyakarta was influenced by arhitectural style from two periods of Indis architecture development in Indonesia, the first one is the period in 1800-1902 (the Empire Style), and the second one is the period in 1902-1920. It most influnced by sosial status of users that reflected on its physical building setting. This sosial status prevailed to inner that reflected on distinct separation between hoodgebouw and bijgebouw, and also the social status prevailed to outside was inter-occupants of the houses location based on level of positions in Centrale Werkplaats (Balai Yasa). Another factor that also influenced the architectural characteristics is local climate that makes this housing looks in characterictics of tropical house. Climate aspect is an essential consideration in Indis house as an effort to create freshness inside the house. Next, combining factors between Ducth architectural forms and the traditional (Java) also influenced the architectural setting. Europe forms seem at building elements like door, window, luifel, gevel, chimney, and floor while the local ones seem at pattern of figure ground, the existence of open space in form of front veranda, the compositition of open space and building, and also vegetation.

Riparian populations of minnesota reed canarygrass (Phalaris arundinacea) are most likely native, based on SNPs (DArTseqLD)

The native vs. exotic status of reed canarygrass (RCG), a major invasive species of Minnesota wetlands, is unknown. The aim of this study was to investigate this native vs. exotic status to enhance its management. Genetic comparison of wild RCG populations from six Minnesota and six Czech Republic rivers was performed. A total of 2521 polymorphic SNP markers (single nucleotide polymorphisms) were used to evaluate 478 RCG samples across all collections. In the PCoA, all (n = 256) tested extant wild, riparian RCG genotypes from six Minnesota Rivers and six Czech Republic Rivers were genetically distinct, although some SNPs were common in both populations since they are the same species. DAPC analysis also resulted in the formation of two primary clusters separating the Minnesota Rivers and Czech Republic Rivers riparian samples, with little overlap; STRUCTURE analysis also supported this clustering with k = 4 groups as it separated the Czech Republic Rivers populations into three groups, along with Minnesota Rivers. The uniformity of PCoA, DAPC, STRUCTURE, and Evanno results indicates the distinct separation of Minnesota Rivers and Czech Republic Rivers populations. Portions of the genome (specific SNPs) are preserved or in common across continents, as indicated by STRUCTURE similarities. Nonetheless, overall significant SNP differences between the continents indicate that the Minnesota riparian populations are distinct enough from the European (Czech) collections to be delineated as native N. American RCG. PCoA of all the Minnesota RCG collections clustered Minnesota Rivers, Herbarium, Extant Herbarium, Research Field and Native Field collections together. STRUCTURE analysis (k = 2; Evanno) divided these Minnesota collections from the Commercial Field and Cultivars collections. There are two genetically distinct groups of RCG in Minnesota and since the Minnesota Rivers, the Research Field, the Native Field and pre-1930 herbaria collections clustered together, they are most likely native N. American types. Analysis of molecular variance (AMOVA) indicated that the genetic variation was more significant within, rather than among, the RCG populations. Native, historic herbaria types cluster together with all wild RCG river populations in Minnesota, all of which were distinct from those in Central Europe, suggesting native RCG type persistence in N. America. Also, cultivated forage types of RCG are distinct from wild RCG Minnesota river populations. The SNP genetic data shows that riparian Minnesota RCG populations are native. These data will facilitate future management strategies to control RCG as a native, but invasive, species.

Small in size, big on taste: metabolomics analysis of flavor compounds from Philippine garlic

Philippine garlic (Allium sativum L.) is arguably known to pack flavor and aroma in smaller bulbs compared to imported varieties saturating the local market. In this study, ethanolic extracts of Philippine garlic cultivars were profiled using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF). γ-Glu dipeptides, oligosaccharides and lipids were determined in Philippine garlic cultivars through bioinformatics analysis in GNPS Molecular Networking Platform and fragmentation analysis. Multivariate statistical analysis using XCMS Online showed the abundance of γ-Glu allyl cysteine in Batanes-sourced garlic while γ-Glu propenyl cysteine, γ-Glu methyl cysteine, and alliin are enriched in the Ilocos cultivar. Principal component analysis showed that the γ-Glu dipeptides found in local garlic influenced their distinct separation across PC1 from imported varieties. This presence of high levels of γ-Glu dipeptides and probiotic oligosaccharides may potentially contribute to the superior flavor and nutritional benefits of Philippine garlic.

Developmental antecedents of cleansing effects: Evidence against domain-generality

Lee and Schwarz propose grounded procedures of separation as a domain-general mechanism underlying cleansing effects. One strong test of domain generality is to investigate the ontogenetic origins of a process. Here, we argue that the developmental evidence provides weak support for a domain-general grounded procedures account. Instead, it is likely that distinct separation procedures develop uniquely for different content domains.