scholarly journals Microscopic Description of Mus Musculus Kidney Preparation Deparafinized with Olive Oil in Eosin (HE) Hematoxylin (HE) Staining

2021 ◽  
Vol 3 (1) ◽  
pp. 61-66
Author(s):  
Ela Nur Pratiwi ◽  
Desy Armalina
Keyword(s):  
Olive Oil ◽  
Mus Musculus ◽  
Visual Fields ◽  
Kidney Tissue ◽  
Carcinogenic Effect ◽  
Microscopic Appearance ◽  
Skin Erythema ◽  
Acute Neurotoxicity ◽  
Descriptive Approach ◽  
Hematoxylin Eosin

Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.

scholarly journals Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif (Penelitian Pendahuluan)

2021 ◽  
Vol 3 (2) ◽  
pp. 67-71
Author(s):  
Eko Naning Sofyanita ◽  
Arya Arya Iswara
Keyword(s):  
Olive Oil ◽  
Visual Fields ◽  
Kidney Tissue ◽  
Carcinogenic Effect ◽  
Microscopic Appearance ◽  
Skin Erythema ◽  
Acute Neurotoxicity ◽  
Descriptive Approach ◽  
Hematoxylin Eosin ◽  
Good Preparation

Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.

scholarly journals Profile Microscopic Profile of Mice (Mus Musculus) Kidney Tissue Fixed with 10% Honey Concentration for 24 Hours

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Tasya Vita Brilian
Keyword(s):  
Mus Musculus ◽  
Visual Fields ◽  
Kidney Tissue ◽  
Chemical Substance ◽  
Original Form ◽  
Microscopic Image ◽  
Study Approach ◽  
Long Time ◽  
Qualitative Descriptive ◽  
Honey Solution

Fixation is used to maintain tissue structure in its original form “life-like state” and can protect proteins and tissue components from degeneration. The solution commonly used is 10% NBF. Formaldehyde is chemical substance that is toxic and not environmentally friendly, several studies have shown alternative substitutes fixation, one of which is the honey solution. The study of Mohammed et al (2020) fixated tissue with honey 10% and 20% shown good coloring properties and similar clarity to fixated with formalin 10%. Honey has acidic and dehydrating properties allow most microorganisms to be killed so that tissues will last for a long time. The research objective is to findout the description of microscopic of mice (Mus musculus) kidney tissue which were fixation using 10% honey solution for 24 hours. The research is included in qualitative descriptive research. The research design used was a non-eksperimental with a purposive sampling study approach. The sample used was 32 preparation with total of microscopic overview is 160. Microscopic image of mice (Mus musculus) kidney tissue fixed using 10% honey solution for 24 hours in 80 visual fields were 12.5% of the preparations is not good and 87.5% is good preparations. The microscopic image of mice (Mus musculus) kidney tissue fixed using 10% NBF is better than of the microscopic image of mice (Mus musculus) kidney tissue fixed with 10% honey for 24 hours.

scholarly journals Lipofuscin Accumulation in Cortisol-Producing Adenomas With and Without PRKACA Mutations

2017 ◽  
Vol 49 (10) ◽  
pp. 786-792 ◽  
Author(s):  
Anna Angelousi ◽  
Eva Szarek ◽  
Vincent Shram ◽  
Electron Kebebew ◽  
Martha Quezado ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Cushing Syndrome ◽  
Visual Fields ◽  
Laser Scanning Microscopy ◽  
Detection Methods ◽  
Confocal Laser ◽  
Lipofuscin Accumulation ◽  
Lipofuscin Pigment ◽  
Lipofuscin Granules ◽  
Hematoxylin Eosin

AbstractThe adrenal cortex accumulates lipofuscin granules with age. Lipofuscin accumulation is also seen in adrenocortical tumors associated with Cushing syndrome (CS), particularly those with PRKAR1A mutations, such as in primary pigmented nodular adrenocortical disease (PPNAD). We investigated the presence of lipofuscin in cortisol-producing adenomas (CPAs) responsible for CS with and without the PRKACA (pLeu206Arg) somatic mutation. Ten paraffin-embedded sections of CPAs from cases with overt CS with (n=4) and without (n=6) a PRKACA mutation were microscopically examined through three detection methods, the hematoxylin-Eosin (H & E) staining, the Fontana Masson (FM) staining using light microscopy, and lipofuscin autofluorescence, using confocal laser scanning microscopy (CLSM). Sections were examined quantitatively according to the intensity of the pigmentation, as well as qualitatively based on the total number of granular pigments at all visual fields per tissue slide. Tissues from CPAs were compared to peritumoral adjacent tissues (n=5), to Conn adenomas (n=4), and PPNAD (n=3). CPAs had significantly higher number of lipofuscin-pigment granules compared to peritumoral adrenal tissue and Conn adenomas (46.9±9.5 vs. 3.8±4.8, p=0.0001). The presence of the PRKACA mutation did not increase the chances of pigmentation in the form of lipofuscin granules within CPAs associated with CS. Thus, all CPAs leading to CS accumulate lipofuscin, which presents like pigmentation sometimes seen macroscopically but always detected microscopically. PPNAD caused by PRKAR1A mutations is the best known adrenal lesion leading to CS associated with intense lipofuscin pigmentation and this was confirmed here; CPAs harboring PRKACA mutations did not have statistically significantly more pigmentation than CPAs without mutation, but a larger study might have shown a difference.

scholarly journals Ekstrak Etanol Moringa Oleifera Lam Terhadap Folikulogenesis Pada Mencit Model Endometriosis

2017 ◽  
Vol 19 (3) ◽  
pp. 246
Author(s):  
Rila Rindi Antina
Keyword(s):  
Mus Musculus ◽  
Moringa Oleifera ◽  
Hematoxylin Eosin ◽  
Moringa Oleifera Lam

Endometriosis didefinisikan sebagai adanya jaringan endometrium di luar rahim.Peningkatan konsentrasi sitokin inflamasi dan faktor pertumbuhan di dalam cairanperitonium berkontribusi pada pembentukan lesi peritonium. Faktor pertumbuhanberperan selama folliculogenesis. Moringa oleifera kaya akan flavonoid. Kuercetin dankaempferol adalah flavonoid, senyawa dengan gugus hidroksil fenolik dengan aksiantioksidan yang memiliki kegunaan potensial terapeutik. Penelitian ini untukmembuktikan adanya pengaruh pemberian ekstrak etanol daun kelor (Moringa oleiferaLam) terhadap folikulogenesis pada mencit (Mus musculus) model endometriosis.Metode penelitian menggunakan mencit (Mus musculus) 20-25g yang dibagi menjadi duakelompok, yaitu kelompok kontrol endometriosis dan kelompok perlakuan diberi ekstraketanol daun kelor 0,35 mg/gBB selama 14 hari. Jaringan ovarium dilakukan pemeriksaanhistopatologi dengan pengecatan hematoxylin-eosin. Hasil penelitian menunjukkanterdapat perbedaan yang signifikan (ρ<0,05) jumlah folikel primer, jumlah folikelsekunder, jumlah folikel tersier, jumlah folikel de graaf pada kelompok kontrol dankelompok perlakuan. Kesimpulan Penelitian menunjukkan ekstrak etanol daun kelormeningkatkan folikulogenesis pada mencit model endometriosisKata kunci—Ekstrak etanol daun kelor, folikulogenesis, endometrosis 

scholarly journals Comparison of olive leaf, olive oil, palm oil, and omega-3 oil in acute kidney injury induced by sepsis in rats

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7219 ◽  
Author(s):  
Maria Fátima de Paula Ramos ◽  
Olvania Basso Oliveira ◽  
Alceni do Carmo Morais Monteiro de Barros ◽  
Clara Versolato Razvickas ◽  
Edson de Andrade Pessoa ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Olive Oil ◽  
Palm Oil ◽  
Interleukin 1 ◽  
Kidney Tissue ◽  
Kidney Injury ◽  
Therapeutic Interventions ◽  
Natural Food ◽  
Omega 3 ◽  
Olive Leaf

BackgroundHypotension, increased production of reactive oxygen species, and inflammation are all observed in experimental models of sepsis induced by lipopolysaccharide (LPS).PurposeThe aim of this study was to evaluate the effects of an ethanolic extract of Brazilian olive leaf (Ex), Brazilian olive oil (Olv), Ex + Olv (ExOlv), and palm oil (Pal) in comparison to the effects of omega-3 fish oil (Omg) in a rat model of sepsis-induced acute kidney injury.MaterialsWistar rats were divided into seven groups (seven per group), which were either untreated (control) or treated with LPS, LPS + Ex, LPS + ExOlv, LPS + Olv, LPS + Omg, or LPS + Pal.ResultsLower values of creatinine clearance and blood pressure were observed in the LPS-treated group, and these values were not affected by Ex, Olv, ExOlv, Pal, or Omg treatment. Mortality rates were significantly lower in rats exposed to LPS when they were also treated with Ex, ExOlv, Olv, Pal, or Omg. These treatments also decreased oxidative stress and inflammation (Tumor necrosis factor alpha, interleukin-1 beta) and increased interleukin-10 levels and cell proliferation, which were associated with decreased apoptosis in kidney tissue.ConclusionEx and Pal treatments were beneficial in septic rats, since they increased survival rate and did not aggravate inflammation. However, the most effective treatments for septic rats were Olv in comparison to Omg. These natural food substances could enable the development of effective therapeutic interventions to sepsis.

scholarly journals Amelorative effect of olive oil against hepatorenal toxicity of cefotaxime in albino rats

2020 ◽  
Vol 8 (1) ◽  
pp. 96
Author(s):  
Ashraf A. A. Elkomy ◽  
Mossad G. E Elsayed ◽  
Faten I. El sayed ◽  
Ahmed A. Abd el atey
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Olive Oil ◽  
Kidney Tissue ◽  
Antioxidant Effect ◽  
Oxidative Status ◽  
Control Group ◽  
Albino Rats ◽  
Total Protein Level ◽  
Liver And Kidney

Due to great hazard effects of antibiotic the following study aimed to investigate the adverse effect of cefotaxime in biochemical, oxidative status and histological examination of Liver and kidney tissue as well as the protective effect of olive oil. Twenty four male Wister albino rats were randomly divided into main four groups including: - G (1): Served as control group and it includes six rats, they were administrated 0.5ml of saline orally for 14 consecutive days. G (2): it includes six rats, they were administered 5ml/kg olive oil orally for 14 consecutive days. G (3): it includes six rats, they were administrated 90mg/kg body weight/twice daily of cefotaxime intramuscular for 14 consecutive days. G (4): it includes six rats, they were administered 5ml/kg olive oil orally concurrently with 90mg/kg body weight/twice daily of cefotaxime. Results revealed that cefotaxime induced significant increases in liver and kidney function parameters including AST, ALT, ALP. creatinine, and urea as well as decrease in albumin and total protein level. Moreover, marked an increase in malondialdehyde (MDA) and decreases in glutathione (GSH) and catalase (CAT) levels. that indicate oxidative stress levels expression in the hepatic and renal tissues following cefotaxime administration. On the beneficial side oral administration of olive oil at the dose 5ml/kg for 14 days significantly mitigate theses toxic effects. So it is concluded that olive oil has great hepatorenal antioxidant effect. 

Ligusticum chuanxiong Effects on Oxidative Stress and Nrf2/HO-1 Signaling Using Nano-Magnetic Beads in Bleomycin-Induced Renal Fibrosis Rats

2020 ◽  
Vol 12 (10) ◽  
pp. 1185-1191
Author(s):  
Haixia Liu ◽  
Wenwen Huang ◽  
Xinli Han ◽  
Qihang Ma
Keyword(s):  
Renal Fibrosis ◽  
Magnetic Beads ◽  
Rat Kidney ◽  
Smooth Muscle Actin ◽  
Kidney Tissue ◽  
Renal Tissue ◽  
High Dose ◽  
Early Administration ◽  
Ligusticum Chuanxiong ◽  
Hematoxylin Eosin

Ligusticum chuanxiong can relieve the degree of renal fibrosis. However, the specific mechanism of Ligusticum chuanxiong to improve renal fibrosis is not yet clear. A unilateral ureteral obstruction was used to construct a rat renal fibrosis model. The rats were treated with 20 mg/kg and 40 mg/kg of Ligusticum chuanxiong. Four weeks after treatment, blood was collected from the rats, and the rats were sacrificed. Blood urea nitrogen (BUN), serum creatinine (Scr), kidney tissue malondialdehyde (MDA), and superoxide dismutase (SOD) levels were detected. Hematoxylin–eosin staining was used to observe the pathological rat kidney changes. The renal tissue smooth muscle actin (α-SMA) was detected by immunohistochemistry. Nrf2 and HO-1 levels were determined by PCR using nano-magnetic beads. The results showed BUN, Scr, and MDA levels reduced, while SOD levels were elevated in Ligusticum chuanxiong-treated rats, compared to model rats (P < 0.05). These effects were more dramatic in Ligusticum chuanxiong high dose (HD) rats compared to Ligusticum chuanxiong low dose (LD) rats. Additionally, Nrf2 and HO-1 levels were elevated in Ligusticum chuanxiong-treated rats (P < 0.05). These effects were also more dramatic in HD rats compared to LD rats. These findings indicated that Ligusticum chuanxiong early administration can reduce renal fibrosis in rats by stimulating the Nrf2/HO-1 pathway.

Histoprotective effect of vitamin D against carbon tetrachloride nephrotoxicity in rats

2015 ◽  
Vol 35 (7) ◽  
pp. 713-723 ◽  
Author(s):  
SMEO Elmubarak ◽  
N Özsoy
Keyword(s):  
Vitamin D ◽  
Carbon Tetrachloride ◽  
Periodic Acid ◽  
Kidney Tissue ◽  
Albino Rats ◽  
Periodic Acid Schiff ◽  
Glucose Levels ◽  
Tissue Sections ◽  
Wistar Albino Rats ◽  
Hematoxylin Eosin

This study investigated the protective effect of vitamin D against carbon tetrachloride (CCl4)-induced nephrotoxicity in rats. Adult male Wistar albino rats were divided into four groups ((A) control; (B) 10-week exposure to CCl4; (C) 10-week exposure to CCl4 + vitamin D treatment; and (D) 10-week exposure to CCl4 + 12 weeks of vitamin D treatment). The CCl4 dose (1.5 ml kg−1) was injected subcutaneously twice a week, while the 0.5 mg kg−1 dose of vitamin D was administered intraperitoneally every day, as appropriate for each group. Whole animal and kidney weights as well as serum urea, creatinine, and glucose levels were measured. Kidney tissue sections were stained with hematoxylin–eosin, Masson’s trichrome, and periodic acid–Schiff. Tubular and glomerular degeneration were detected in the kidney tissues of CCl4-treated rats, together with dilatation and vacuolization within the tubules and hemorrhage in the intertubular region. In the kidney glomeruli; congestion, atrophy, and adhesion to parietal layer were observed. Tissue disorganization and aggregation of Bowman’s capsules were noted. Mononuclear cell infiltration was observed between the glomeruli and the tubules. In contrast, the kidney sections and functional parameters of vitamin D-treated rats were similar to the controls, suggesting that vitamin D treatment is able to reduce renal damage.

scholarly journals Pengaruh Meniran Dosis Bertingkat Terhadap Ekspresi IGF-1 Dan Ketebalan Endometrium Pada Mencit Betina Model Endometriosis

2018 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Eka Deviany Widyawaty
Keyword(s):  
Mus Musculus ◽  
Phyllanthus Niruri ◽  
Hematoxylin Eosin

Abstrak Endometriosis menyebabkan 10-15% dari nyeri panggul dan infertilitas pada wanita usia reproduksi. Walaupun endometriosis dapat mempengaruhi infertilitas, tetapi mekanisme dari hal tersebut sampai sekarang masih belum diketahui secara pasti. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol herba meniran (Phyllanthus niruri L.) dosis bertingkat terhadap ekspresi Igf-1 dan ketebalan endometrium pada mencit (Mus musculus) betina model endometriosis. Sampel penelitian ini adalah mencit (Mus Musculus) betina model endometriosis. Pada hari ke-15, Kelompok Kontrol (K1) diberi Na CMC 0,5 % dan Kelompok perlakuan (K2), (K3), (K4) diberi ekstrak herba etanol herba meniran dosis 14 mg/20 gBB, 28 mg/20gBB, dan 56 mg/20gBB. Pada hari ke-29 dilakukan pembedahan dan pengambilan sampel endometrium dengan pemeriksaan imunohistokia pada ekspresi IGF-1 dan pewarnaan Hematoxylin-eosin pada ketebalan endometrium. Uji statistik menunjukkan bahwa ada pengaruh pemberian ekstrak etanol herba meniran (Phyllantinus niruri L.) dosis bertingkat terhadap ekspresi IGF-1 dengan nilai p = 0,041 < 0,05 dan ada pengaruh pemberian ekstrak etanol herba meniran (Phyllantinus niruri L.) dosis bertingkat terhadap ketebalan endometrium dengan nilai p = 0,021 < 0,05. Pemberian ekstrak etanol herba meniran (Phyllanthus niruri L.) dosis bertingkat pada mencit (Mus musculus) betina  model endometriosis dapat meningkatkan ekspresi Igf-1 dan ketebalan endometriosis.  Kata Kunci        :    Ekstrak etanol herba meniran, ekspresi IGF-1, ketebalan endometrium

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